LOCUS       sequence-388904-        7241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ATG9A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   De Pace R, Skirzewski M, Damme M, Mattera R, Mercurio J, Foster AM, 
            Cuitino L, Jarnik M, Hoffmann V, Morris HD, Han TU, Mancini GMS, 
            Buonanno A, Bonifacino JS
  TITLE     Altered distribution of ATG9A and accumulation of axonal aggregates 
            in neurons from a mouse model of AP-4 deficiency syndrome.
  JOURNAL   PLoS Genet. 2018 Apr 26;14(4):e1007363. doi: 
            10.1371/journal.pgen.1007363. eCollection 2018 Apr.
  PUBMED    29698489
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2018-04-26"; volume: "14(4):e1007363. doi"; pages: "
            10.1371/journal.pgen.1007363. eCollection 2018 Apr"
COMMENT     Sequence Label: ATG9A-mCherry
FEATURES             Location/Qualifiers
     source          1..7241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3893..3902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3899..4609
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4044..4062)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4312..4331
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4732..4853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4769..4788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4823..4842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4860..5315)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4997..5018)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5209..5228
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5342..5446
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5448..5805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5448..5639
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5471..5491)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5656..5791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5840..6634
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5840..6634
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5894..5913)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6504..6523
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6822..6841)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6866..6913
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
     1321 gctcaagctt cgaattctga tggcgcagtt cgacactgaa taccagcgcc tagaggcctc
     1381 ctatagtgat tcacccccag gggaggagga cctgttggtg cacgtcgccg aggggagcaa
     1441 gtcaccttgg caccatattg aaaaccttga cctcttcttc tctcgagttt ataatctgca
     1501 ccagaagaat ggcttcacat gtatgctcat cggggagatc tttgagctca tgcagttcct
     1561 ctttgtggtt gccttcacta ccttcctggt cagctgcgtg gactatgaca tcctatttgc
     1621 caacaagatg gtgaaccaca gtcttcaccc tactgaaccc gtcaaggtca ctctgccaga
     1681 cgcctttttg cctgctcaag tctgtagtgc caggattcag gaaaatggct cccttatcac
     1741 catcctggtc attgctggtg tcttctggat ccaccggctt atcaagttca tctataacat
     1801 ttgctgctac tgggagatcc actccttcta cctgcacgct ctgcgcatcc ctatgtctgc
     1861 ccttccgtat tgcacgtggc aagaagtgca ggcccggatc gtgcagacgc agaaggagca
     1921 ccagatctgc atccacaaac gtgagctgac agaactggac atctaccacc gcatcctccg
     1981 tttccagaac tacatggtgg cactggttaa caaatccctc ctgcctctgc gcttccgcct
     2041 gcctggcctc ggggaagctg tcttcttcac ccgtggtctc aagtacaact ttgagctgat
     2101 cctcttctgg ggacctggct ctctgtttct caatgaatgg agcctcaagg ccgagtacaa
     2161 acgtgggggg caacggctag agctggccca gcgcctcagc aaccgcatcc tgtggattgg
     2221 catcgctaac ttcctgctgt gccccctcat cctcatatgg caaatcctct atgccttctt
     2281 cagctatgct gaggtgctga agcgggagcc gggggccctg ggagcacgct gctggtcact
     2341 ctatggccgc tgctacctcc gccacttcaa cgagctggag cacgagctgc agtcccgcct
     2401 caaccgtggc tacaagcccg cctccaagta catgaattgc ttcttgtcac ctcttttgac
     2461 actgctggcc aagaatggag ccttcttcgc tggctccatc ctggctgtgc ttattgccct
     2521 caccatttat gacgaagatg tgttggctgt ggaacatgtg ctgaccaccg tcacactcct
     2581 gggggtcacc gtgaccgtgt gcaggtcctt tatcccggac cagcacatgg tgttctgccc
     2641 tgagcagctg ctccgcgtga tcctcgctca catccactac atgcctgacc actggcaggg
     2701 taatgcccac cgctcgcaga cccgggacga gtttgcccag ctcttccagt acaaggcagt
     2761 gttcattttg gaagagttgc tgagccccat tgtcacaccc ctcatcctca tcttctgcct
     2821 gcgcccacgg gccctggaga ttatagactt cttccgaaac ttcaccgtgg aggtcgttgg
     2881 tgtgggagat acctgctcct ttgctcagat ggatgttcgc cagcatggtc atccccagtg
     2941 gctatctgct gggcagacag aggcctcagt gtaccagcaa gctgaggatg gaaagacaga
     3001 gttgtcactc atgcactttg ccatcaccaa ccctggctgg cagccaccac gtgagagcac
     3061 agccttccta ggcttcctca aggagcaggt tcagcgggat ggagcagctg ctagcctcgc
     3121 ccaagggggt ctgctccctg aaaatgccct ctttacgtct atccagtcct tacaatctga
     3181 gtctgagccc ctgagcctta tcgcaaatgt ggtagctggc tcatcctgcc ggggccctcc
     3241 actgcccaga gacctgcagg gctccaggca cagggctgaa gtcgcctctg ccctgcgctc
     3301 cttctccccg ctgcaacccg ggcaggcgcc cacaggccgg gctcacagca ccatgacagg
     3361 ctctggggtg gatgccagga cagccagctc cgggagcagc gtgtgggaag gacagctgca
     3421 gagcctggtg ctgtcagaat atgcatccac agagatgagc ctgcatgccc tctatatgca
     3481 ccagctccac aagcagcagg cccaggctga acctgagcgg catgtatggc accgccggga
     3541 gagtgatgag agtggagaaa gcgcccctga tgaaggggga gagggcgccc gggcccccca
     3601 gtctatccct cgctctgcta gctatccctg tgcagcaccc cggcctggag ctcctgagac
     3661 caccgccctg catgggggct tccagaggcg ctacggtggc atcacagatc ctggcacagt
     3721 gcccagggtt ccctctcatt tctctcggct gcctcttgga gggtgggcag aagatgggca
     3781 gtcggcatca aggcaccctg agcccgtgcc cgaagagggc tcggaggatg agctaccccc
     3841 tcaggtgcac aaggtaacgt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
     3901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4321 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga
     4621 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     4681 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     4861 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     4921 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     4981 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     5041 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5101 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5161 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5221 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5281 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     5341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     5461 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5521 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5581 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5641 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5701 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5761 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     5821 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5881 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5941 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6001 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6061 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6121 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6181 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6241 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6301 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6361 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6421 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6481 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6541 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6601 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6661 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6721 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6781 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     6841 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     6901 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6961 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     7021 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     7081 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
