LOCUS       sequence-388921-       22664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The targeting construct contains a CAGG promoter, FRT site, 
            loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter,
            WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22664)
  AUTHORS   Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, 
            Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, 
            Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin
            L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger 
            S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T
  TITLE     A prime editor mouse to model a broad spectrum of somatic mutations 
            in vivo.
  JOURNAL   Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y.
  PUBMED    37169967
REFERENCE   2  (bases 1 to 22664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y."
COMMENT     Sequence Label: Rosa26-LSL-PE2 Targeting Vector
FEATURES             Location/Qualifiers
     source          1..22664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1764..2780
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2704..2726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2788..2807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2856..2889
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2913..2946
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3113..3247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3367..3501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3621..3755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3784..3817
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3912..3932
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3933..6446,6447..6449,6450..8033)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3933..8033
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10206..10226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10236..10292
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10293..11003
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      10548..10557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11042..11630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11095..11115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11513..11524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11633..11649)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    11657..11864
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    11872..11905
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        11927..12426
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    12446..12479
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      12480..12489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12486..13289
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12549..12568)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13159..13178
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13299..13760
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    13826..18166
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        18216..18715
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             18742..19314
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          19391..19456
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    19530..19754
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(19816..19836)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19816..19834)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19855..19871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19855..19871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(19868..19890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19879..19895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19903..19909,19910..19927,19928..19933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19948..19969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(20086..20103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(20257..20845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20337..20356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(21016..21807,21808..21876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21639..21658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21877..21981)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      22008..22463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(22095..22114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     22305..22326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     22589..22611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     22603..22620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     22604..22620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     22630..22649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22630..22648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg
      421 gggggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccrggsg
     1621 gggsggggsg gggsgrgggg sggggsgggg sgrggsggrg aggkgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     1801 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1861 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1921 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     1981 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2041 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2101 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2161 acaaaggctg cgtgcggggt gtgtgcgtgg gggggkgagc agggggtgtg ggcgcgtcgg
     2221 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2281 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     2341 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     2401 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2461 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     2521 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     2581 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     2641 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     2701 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2761 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2821 ttggcaaaga attgatttga taccgcgggc cctaagaagt tcctattctc tagaaagtat
     2881 aggaacttcg tcgacattta aatcatttaa atataacttc gtataatgta tgctatacga
     2941 agttattcgc gatgaataaa tgaaagcttg cagatctgcg actctagagg atctgcgact
     3001 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3061 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3121 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3181 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3241 ctggatctgc gactctagag gatcataatc agccatacca catttgtaga ggttttactt
     3301 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3361 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3481 gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg
     3541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3721 ccaaactcat caatgtatct tatcatgtct ggatccccat caagctgatc cggaaccctt
     3781 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgac ctgcagccca
     3841 agctagatcg aattcggccg gccttcgaag ccaccatgaa acggacagcc gacggaagcg
     3901 agttcgagtc accaaagaag aagcggaaag tcgacaagaa gtacagcatc ggcctggaca
     3961 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     4021 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     4081 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     4141 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     4201 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     4261 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     4321 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     4381 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     4441 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     4501 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     4561 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     4621 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     4681 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     4741 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     4801 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     4861 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     4921 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     4981 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     5041 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     5101 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     5161 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     5221 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     5281 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     5341 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     5401 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     5461 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     5521 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     5581 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     5641 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     5701 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     5761 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     5821 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     5881 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     5941 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     6001 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     6061 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     6121 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     6181 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     6241 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     6301 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     6361 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     6421 accggctgtc cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact
     6481 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     6541 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     6601 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     6661 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     6721 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     6781 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     6841 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     6901 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     6961 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     7021 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     7081 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     7141 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     7201 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     7261 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     7321 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     7381 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     7441 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     7501 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     7561 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     7621 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     7681 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     7741 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     7801 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     7861 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     7921 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     7981 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt gactctggag
     8041 gatctagcgg aggatcctct ggcagcgaga caccaggaac aagcgagtca gcaacaccag
     8101 agagcagtgg cggcagcagc ggcggcagca gcaccctaaa tatagaagat gagtatcggc
     8161 tacatgagac ctcaaaagag ccagatgttt ctctagggtc cacatggctg tctgattttc
     8221 ctcaggcctg ggcggaaacc gggggcatgg gactggcagt tcgccaagct cctctgatca
     8281 tacctctgaa agcaacctct acccccgtgt ccataaaaca ataccccatg tcacaagaag
     8341 ccagactggg gatcaagccc cacatacaga gactgttgga ccagggaata ctggtaccct
     8401 gccagtcccc ctggaacacg cccctgctac ccgttaagaa accagggact aatgattata
     8461 ggcctgtcca ggatctgaga gaagtcaaca agcgggtgga agacatccac cccaccgtgc
     8521 ccaaccctta caacctcttg agcgggctcc caccgtccca ccagtggtac actgtgcttg
     8581 atttaaagga tgcctttttc tgcctgagac tccaccccac cagtcagcct ctcttcgcct
     8641 ttgagtggag agatccagag atgggaatct caggacaatt gacctggacc agactcccac
     8701 agggtttcaa aaacagtccc accctgttta atgaggcact gcacagagac ctagcagact
     8761 tccggatcca gcacccagac ttgatcctgc tacagtacgt ggatgactta ctgctggccg
     8821 ccacttctga gctagactgc caacaaggta ctcgggccct gttacaaacc ctagggaacc
     8881 tcgggtatcg ggcctcggcc aagaaagccc aaatttgcca gaaacaggtc aagtatctgg
     8941 ggtatcttct aaaagagggt cagagatggc tgactgaggc cagaaaagag actgtgatgg
     9001 ggcagcctac tccgaagacc cctcgacaac taagggagtt cctagggaag gcaggcttct
     9061 gtcgcctctt catccctggg tttgcagaaa tggcagcccc cctgtaccct ctcaccaaac
     9121 cggggactct gtttaattgg ggcccagacc aacaaaaggc ctatcaagaa atcaagcaag
     9181 ctcttctaac tgccccagcc ctggggttgc cagatttgac taagcccttt gaactctttg
     9241 tcgacgagaa gcagggctac gccaaaggtg tcctaacgca aaaactggga ccttggcgtc
     9301 ggccggtggc ctacctgtcc aaaaagctag acccagtagc agctgggtgg cccccttgcc
     9361 tacggatggt agcagccatt gccgtactga caaaggatgc aggcaagcta accatgggac
     9421 agccactagt cattctggcc ccccatgcag tagaggcact agtcaaacaa ccccccgacc
     9481 gctggctttc caacgcccgg atgactcact atcaggcctt gcttttggac acggaccggg
     9541 tccagttcgg accggtggta gccctgaacc cggctacgct gctcccactg cctgaggaag
     9601 ggctgcaaca caactgcctt gatatcctgg ccgaagccca cggaacccga cccgacctaa
     9661 cggaccagcc gctcccagac gccgaccaca cctggtacac ggatggaagc agtctcttac
     9721 aagagggaca gcgtaaggcg ggagctgcgg tgaccaccga gaccgaggta atctgggcta
     9781 aagccctgcc agccgggaca tccgctcagc gggctgaact gatagcactc acccaggccc
     9841 taaagatggc agaaggtaag aagctaaatg tttatactga tagccgttat gcttttgcta
     9901 ctgcccatat ccatggagaa atatacagaa ggcgtgggtg gctcacatca gaaggcaaag
     9961 agatcaaaaa taaagacgag atcttggccc tactaaaagc cctctttctg cccaaaagac
    10021 ttagcataat ccattgtcca ggacatcaaa agggacacag cgccgaggct agaggcaacc
    10081 ggatggctga ccaagcggcc cgaaaggcag ccatcacaga gactccagac acctctaccc
    10141 tcctcataga aaattcatca ccctctggcg gctcaaaaag aaccgccgac ggcagcgaat
    10201 tcgagcccaa gaagaagagg aaagtcggca gcggcgccac caacttcagc ctgctgaagc
    10261 aggccggcga cgtggaggag aaccccggcc ccatggtgag caagggcgag gaggataaca
    10321 tggcctctct cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact
    10381 ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga
    10441 agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg
    10501 gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag
    10561 atggctccgg ataccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg
    10621 ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga
    10681 ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca
    10741 ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca
    10801 ccactggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc
    10861 caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca
    10921 agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg
    10981 gcatggacga gctgtacaag tgaggccggc cctgcaggaa ttcgatatca agcttatcga
    11041 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    11101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    11161 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    11221 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    11281 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    11341 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    11401 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
    11461 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    11521 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    11581 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
    11641 tcgacctcga cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    11701 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    11761 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    11821 agcaaggggg aggattggga agacaatggc aggcatgctg gggaactagt ggtgccaggg
    11881 cgtgcccttg ggctccccgg gcgcggcggc cgcatcgaat tctaccgggt aggggaggcg
    11941 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
    12001 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
    12061 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
    12121 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
    12181 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
    12241 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
    12301 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
    12361 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
    12421 ctttcgacct gcaatcgccg ctagcgaagt tcctattctt caaatagtat aggaacttcg
    12481 ccaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    12541 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    12601 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    12661 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    12721 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    12781 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    12841 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    12901 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    12961 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    13021 cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    13081 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    13141 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    13201 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    13261 tctatcgcct tcttgacgag ttcttctgag gggatccgct gtaagtctgc agaaattgat
    13321 gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta
    13381 agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg
    13441 ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact attgctttat
    13501 gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc
    13561 tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct
    13621 cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc
    13681 tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc
    13741 aagttctaat tccatcagaa agcttgcaga agatctcccc aactggggta acctttgagt
    13801 tctctcagtt gggggggcgc gccggctaga agatgggcgg gagtcttctg ggcaggctta
    13861 aaggctaacc tggtgtgtgg gcgttgtcct gcaggggaat tgaacaggtg taaaattgga
    13921 gggacaagac ttcccacaga ttttcggttt tgtcgggaag ttttttaata ggggcaaata
    13981 aggaaaatgg gaggataggt agtcatctgg ggttttatgc agcaaaacta caggttatta
    14041 ttgcttgtga tccgcctcgg agtattttcc atcgaggtag attaaagaca tgctcacccg
    14101 agttttatac tctcctgctt gagatcctta ctacagtatg aaattacagt gtcgcgagtt
    14161 agactatgta agcagaattt taatcatttt taaagagccc agtacttcat atccatttct
    14221 cccgctcctt ctgcagcctt atcaaaaggt attttagaac actcatttta gccccatttt
    14281 catttattat actggcttat ccaaccccta gacagagcat tggcattttc cctttcctga
    14341 tcttagaagt ctgatgactc atgaaaccag acagattagt tacatacacc acaaatcgag
    14401 gctgtagctg gggcctcaac actgcagttc ttttataact ccttagtaca ctttttgttg
    14461 atcctttgcc ttgatcctta attttcagtg tctatcacct ctcccgtcag gtggtgttcc
    14521 acatttgggc ctattctcag tccagggagt tttacaacaa tagatgtatt gagaatccaa
    14581 cctaaagctt aactttccac tcccatgaat gcctctctcc tttttctcca tttataaact
    14641 gagctattaa ccattaatgg tttccaggtg gatgtctcct cccccaatat tacctgatgt
    14701 atcttacata ttgccaggct gatattttaa gacattaaaa ggtatatttc attattgagc
    14761 cacatggtat tgattactgc ttactaaaat tttgtcattg tacacatctg taaaaggtgg
    14821 ttccttttgg aatgcaaagt tcaggtgttt gttgtctttc ctgacctaag gtcttgtgag
    14881 cttgtatttt ttctatttaa gcagtgcttt ctcttggact ggcttgactc atggcattct
    14941 acacgttatt gctggtctaa atgtgatttt gccaagcttc ttcaggacct ataattttgc
    15001 ttgacttgta gccaaacaca agtaaaatga ttaagcaaca aatgtatttg tgaagcttgg
    15061 tttttaggtt gttgtgttgt gtgtgcttgt gctctataat aatactatcc aggggctgga
    15121 gaggtggctc ggagttcaag agcacagact gctcttccag aagtcctgag ttcaattccc
    15181 agcaaccaca tggtggctca caaccatctg taatgggatc tgatgccctc ttctggtgtg
    15241 tctgaagacc acaagtgtat tcacattaaa taaataaatc ctccttcttc ttcttttttt
    15301 tttttttaaa gagaatactg tctccagtag aatttactga agtaatgaaa tactttgtgt
    15361 ttgttccaat atggtagcca ataatcaaat tactctttaa gcactggaaa tgttaccaag
    15421 gaactaattt ttatttgaag tgtaactgtg gacagaggag ccataactgc agacttgtgg
    15481 gatacagaag accaatgcag actttaatgt cttttctctt acactaagca ataaagaaat
    15541 aaaaattgaa cttctagtat cctatttgtt taaactgcta gctttactta acttttgtgc
    15601 ttcatctata caaagctgaa agctaagtct gcagccatta ctaaacatga aagcaagtaa
    15661 tgataatttt ggatttcaaa aatgtagggc cagagtttag ccagccagtg gtggtgcttg
    15721 cctttatgcc tttaatccca gcactctgga ggcagagaca ggcagatctc tgagtttgag
    15781 cccagcctgg tctacacatc aagttctatc taggatagcc aggaatacac acagaaaccc
    15841 tgttggggag gggggctctg agatttcata aaattataat tgaagcattc cctaatgagc
    15901 cactatggat gtggctaaat ccgtctacct ttctgatgag atttgggtat tattttttct
    15961 gtctctgctg ttggttgggt cttttgacac tgtgggcttt ctttaaagcc tccttcctgc
    16021 catgtggtct cttgtttgct actaacttcc catggcttaa atggcatggc tttttgcctt
    16081 ctaagggcag ctgctgagat ttgcagcctg atttccaggg tggggttggg aaatctttca
    16141 aacactaaaa ttgtccttta attttttttt taaaaaatgg gttatataat aaacctcata
    16201 aaatagttat gaggagtgag gtggactaat attaaatgag tccctcccct ataaaagagc
    16261 tattaaggct ttttgtctta tacttaactt tttttttaaa tgtggtatct ttagaaccaa
    16321 gggtcttaga gttttagtat acagaaactg ttgcatcgct taatcagatt ttctagtttc
    16381 aaatccagag aatccaaatt cttcacagcc aaagtcaaat taagaatttc tgacttttaa
    16441 tgttaatttg cttactgtga atataaaaat gatagctttt cctgaggcag ggtctcacta
    16501 tgtatctctg cctgatctgc aacaagatat gtagactaaa gttctgcctg cttttgtctc
    16561 ctgaatacta aggttaaaat gtagtaatac ttttggaact tgcaggtcag attcttttat
    16621 aggggacaca ctaagggagc ttgggtgata gttggtaaaa tgtgtttcaa gtgatgaaaa
    16681 cttgaattat tatcaccgca acctactttt taaaaaaaaa agccaggcct gttagagcat
    16741 gcttaaggga tccctaggac ttgctgagca cacaagagta gttacttggc aggctcctgg
    16801 tgagagcata tttcaaaaaa caaggcagac aaccaagaaa ctacagttaa ggttacctgt
    16861 ctttaaacca tctgcatata cacagggata ttaaaatatt ccaaataata tttcattcaa
    16921 gttttccccc atcaaattgg gacatggatt tctccggtga ataggcagag ttggaaacta
    16981 aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag tgatagttaa agcccatgag
    17041 atacagaaca aagctgctat ttcgaggtct cttggtttat actcagaagc acttctttgg
    17101 gtttccctgc actatcctga tcatgtgcta ggcctacctt aggctgattg ttgttcaaat
    17161 aaacttaagt ttcctgtcag gtgatgtcat atgatttcat atatcaaggc aaaacatgtt
    17221 atatatgtta aacatttgta cttaatgtga aagttaggtc tttgtgggtt tgatttttaa
    17281 ttttcaaaac ctgagctaaa taagtcattt ttacatgtct tacatttggt ggaattgtat
    17341 aattgtggtt tgcaggcaag actctctgac ctagtaaccc tacctataga gcactttgct
    17401 gggtcacaag tctaggagtc aagcatttca ccttgaagtt gagacgtttt gttagtgtat
    17461 actagtttat atgttggagg acatgtttat ccagaagata ttcaggacta tttttgactg
    17521 ggctaaggaa ttgattctga ttagcactgt tagtgagcat tgagtggcct ttaggcttga
    17581 attggagtca cttgtatatc tcaaataatg ctggcctttt ttaaaaagcc cttgttcttt
    17641 atcaccctgt tttctacata atttttgttc aaagaaatac ttgtttggat ctccttttga
    17701 caacaatagc atgttttcaa gccatatttt ttttcctttt tttttttttt tttggttttt
    17761 cgagacaggg tttctctgta tagccctggc tgtcctggaa ctcactttgt agaccaggct
    17821 ggcctcgaac tcagaaatcc gcctgcctct gcctcctgag tgccgggatt aaaggcgtgc
    17881 accaccacgc ctggctaagt tggatatttt gttatataac tataaccaat actaactcca
    17941 ctgggtggat ttttaattca gtcagtagtc ttaagtggtc tttattggcc cttcattaaa
    18001 atctactgtt cactctaaca gaggctgttg gtactagtgg cacttaagca acttcctacg
    18061 gatatactag cagattaagg gtcagggata gaaactagtc tagcgttttg tatacctacc
    18121 agctttatac taccttgttc tgatagaaat atttcaggac atctagctta tcgataccgt
    18181 cgacggtatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc ttttcccaag
    18241 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
    18301 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
    18361 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
    18421 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
    18481 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
    18541 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
    18601 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
    18661 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
    18721 caggtcctcg ccatggatcc tgatgatgtt gttgattctt ctaaatcttt tgtgatggaa
    18781 aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca aaaaggtata
    18841 caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg gttttatagt
    18901 accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc gctctctgga
    18961 aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct cgcactaaaa
    19021 gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga accgttgatg
    19081 gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc gcgtgtagtg
    19141 ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa ctgggaacag
    19201 gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa acgtggccaa
    19261 gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag gcgatctctt
    19321 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    19381 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
    19441 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
    19501 agatcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    19561 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    19621 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    19681 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    19741 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgaggg ggggcccggt
    19801 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
    19861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    19921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    19981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    20041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    20101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    20161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    20221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    20281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    20341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    20401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    20461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    20521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    20581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    20641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    20701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    20761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    20821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    20881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    20941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    21001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    21061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    21121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    21181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    21241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    21301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    21361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    21421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    21481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    21541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    21601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    21661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    21721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    21781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    21841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    21901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    21961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
    22021 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    22081 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    22141 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    22201 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    22261 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    22321 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    22381 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    22441 caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg
    22501 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    22561 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    22621 gagcgcgcgt aatacgactc actatagggc gaattggagc tcca
//
