LOCUS       sequence-388928-       19318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains a seamless deletion cassette for the replacement of ORF2230
            in D. radiodurans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19318)
  AUTHORS   Brumwell SL, Van Belois KD, Nucifora DP, Karas BJ
  TITLE     SLICER: A Seamless Gene Deletion Method for Deinococcus radiodurans.
  JOURNAL   Biodes Res. 2023 Mar 15;5:0009. doi: 10.34133/bdr.0009. eCollection 
            2023.
  PUBMED    37849465
REFERENCE   2  (bases 1 to 19318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2023-03-15"; volume: "5:0009. doi"; pages: " 
            10.34133/bdr.0009. eCollection 2023"
COMMENT     Sequence Label: pSD5
FEATURES             Location/Qualifiers
     source          1..19318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..628
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      105..636
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      704..923
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1014..1769
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1772..2022
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2184..2687
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2688..2874
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2875..3537
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIGEATSPNGTNDVPSTKGVLM"
     CDS             3727..4902
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4902..5873
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5946..6419
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6679..7077
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7095..7128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7196..7215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7295..7570)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7408..7430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7422..7439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7423..7439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7446..7465
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7446..7464
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7467..7523
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7566..7582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7566..7582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7579..7601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7590..7606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7614..7620,7621..7638,7639..7644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7900..8559)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8474..8493
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8560..8662)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(9694..10344)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             10450..11649
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(12992..13786)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13103..13122)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13713..13732
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        14142..14176
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             14198..14209
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      17347..17418
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      17434..17461
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            18683..18792
                     /note="incP origin of transfer"
     CDS             18825..19196
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ttaattaatc gagctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa
       61 cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccggccgc cggcgttgtg
      121 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
      181 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
      241 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
      301 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
      361 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
      421 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
      481 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
      541 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
      601 ggggtgcccc cccttctcga accctcccgg tcgagtgagc gaggaagcac cagggaacag
      661 cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg ttatccactt
      721 atccacgggg atatttttat aattattttt tttatagttt ttagatcttc ttttttagag
      781 cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa attgcccttt
      841 cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc cttaaccctg
      901 tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct tattgactct
      961 tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct gtcatggcgg
     1021 aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc cagtcaaacg
     1081 acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg tatctgttcg
     1141 ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc tgcgagatcc
     1201 atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt aaggatatac
     1261 ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct gaagaggatg
     1321 ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg cacagtccat
     1381 ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt atcgggttac
     1441 agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat ccgtatgcca
     1501 tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc atcgtctctc
     1561 tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag cgtatgcctg
     1621 acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga actccaatgc
     1681 gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta ttttccttcc
     1741 gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag atttgagggt
     1801 ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg ctgtttcctt
     1861 cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag ccaaatttga
     1921 gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc tgatatcggg
     1981 ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct gaattggcta
     2041 tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc ttgcgctgag
     2101 cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg ctcgctatgc
     2161 tcggttacac ggctgcggcg agcatcacgt gctataaaaa taattataat ttaaattttt
     2221 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     2281 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttacc
     2341 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     2401 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     2461 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     2521 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     2581 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     2641 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccta gtacactcta
     2701 tattttttta tgcctcggta atgattttca tttttttttt ccacctagcg gatgactctt
     2761 tttttttctt agcgattggc attatcacat aatgaattat acattatata aagtaatgtg
     2821 atttcttcga agaatatact aaaaaatgag caggcaagat aaacgaaggc aaagatgaca
     2881 gagcagaaag ccctagtaaa gcgtattaca aatgaaacca agattcagat tgcgatctct
     2941 ttaaagggtg gtcccctagc gatagagcac tcgatcttcc cagaaaaaga ggcagaagca
     3001 gtagcagaac aggccacaca atcgcaagtg attaacgtcc acacaggtat agggtttctg
     3061 gaccatatga tacatgctct ggccaagcat tccggctggt cgctaatcgt tgagtgcatt
     3121 ggtgacttac acatagacga ccatcacacc actgaagact gcgggattgc tctcggtcaa
     3181 gcttttaaag aggccctagg ggccgtgcgt ggagtaaaaa ggtttggatc aggatttgcg
     3241 cctttggatg aggcactttc cagagcggtg gtagatcttt cgaacaggcc gtacgcagtt
     3301 gtcgaacttg gtttgcaaag ggagaaagta ggagatctct cttgcgagat gatcccgcat
     3361 tttcttgaaa gctttgcaga ggctagcaga attaccctcc acgttgattg tctgcgaggc
     3421 aagaatgatc atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc cataggagaa
     3481 gccacctcgc ccaatggtac caacgatgtt ccctccacca aaggtgttct tatgtagttt
     3541 tacacaggag tctggacttg acgctagtga taataagtga ctgaggtatg tgctcttctt
     3601 atctcctttt gtagtgttgc tcttatttta aacaactttg cggttttttg atgactttgc
     3661 gattttgttg ttgctttgca gtaaattgca agatttaata aaaaaacgca aagcaatgat
     3721 taaaggatgt tcagaatgaa actcatggaa acacttaacc agtgcataaa cgctggtcat
     3781 gaaatgacga aggctatcgc cattgcacag tttaatgatg acagcccgga agcgaggaaa
     3841 ataacccggc gctggagaat aggtgaagca gcggatttag ttggggtttc ttctcaggct
     3901 atcagagatg ccgagaaagc agggcgacta ccgcacccgg atatggaaat tcgaggacgg
     3961 gttgagcaac gtgttggtta tacaattgaa caaattaatc atatgcgtga tgtgtttggt
     4021 acgcgattgc gacgtgctga agacgtattt ccaccggtga tcggggttgc tgcccataaa
     4081 ggtggcgttt acaaaacctc agtttctgtt catcttgctc aggatctggc tctgaagggg
     4141 ctacgtgttt tgctcgtgga aggtaacgac ccccagggaa cagcctcaat gtatcacgga
     4201 tgggtaccag atcttcatat tcatgcagaa gacactctcc tgcctttcta tcttggggaa
     4261 aaggacgatg tcacttatgc aataaagccc acttgctggc cggggcttga cattattcct
     4321 tcctgtctgg ctctgcaccg tattgaaact gagttaatgg gcaaatttga tgaaggtaaa
     4381 ctgcccaccg atccacacct gatgctccga ctggccattg aaactgttgc tcatgactat
     4441 gatgtcatag ttattgacag cgcgcctaac ctgggtatcg gcacgattaa tgtcgtatgt
     4501 gctgctgatg tgctgattgt tcccacgcct gctgagttgt ttgactacac ctccgcactg
     4561 cagtttttcg atatgcttcg tgatctgctc aagaacgttg atcttaaagg gttcgagcct
     4621 gatgtacgta ttttgcttac caaatacagc aatagcaatg gctctcagtc cccgtggatg
     4681 gaggagcaaa ttcgggatgc ctggggaagc atggttctaa aaaatgttgt acgtgaaacg
     4741 gatgaagttg gtaaaggtca gatccggatg agaactgttt ttgaacaggc cattgatcaa
     4801 cgctcttcaa ctggtgcctg gagaaatgct ctttctattt gggaacctgt ctgcaatgaa
     4861 attttcgatc gtctgattaa accacgctgg gagattagat aatgaagcgt gcgcctgtta
     4921 ttccaaaaca tacgctcaat actcaaccgg ttgaagatac ttcgttatcg acaccagctg
     4981 ccccgatggt ggattcgtta attgcgcgcg taggagtaat ggctcgcggt aatgccatta
     5041 ctttgcctgt atgtggtcgg gatgtgaagt ttactcttga agtgctccgg ggtgatagtg
     5101 ttgagaagac ctctcgggta tggtcaggta atgaacgtga ccaggagctg cttactgagg
     5161 acgcactgga tgatctcatc ccttcttttc tactgactgg tcaacagaca ccggcgttcg
     5221 gtcgaagagt atctggtgtc atagaaattg ccgatgggag tcgccgtcgt aaagctgctg
     5281 cacttaccga aagtgattat cgtgttctgg ttggcgagct ggatgatgag cagatggctg
     5341 cattatccag attgggtaac gattatcgcc caacaagtgc ttatgaacgt ggtcagcgtt
     5401 atgcaagccg attgcagaat gaatttgctg gaaatatttc tgcgctggct gatgcggaaa
     5461 atatttcacg taagattatt acccgctgta tcaacaccgc caaattgcct aaatcagttg
     5521 ttgctctttt ttctcacccc ggtgaactat ctgcccggtc aggtgatgca cttcaaaaag
     5581 cctttacaga taaagaggaa ttacttaagc agcaggcatc taaccttcat gagcagaaaa
     5641 aagctggggt gatatttgaa gctgaagaag ttatcactct tttaacttct gtgcttaaaa
     5701 cgtcatctgc atcaagaact agtttaagct cacgacatca gtttgctcct ggagcgacag
     5761 tattgtataa gggcgataaa atggtgctta acctggacag gtctcgtgtt ccaactgagt
     5821 gtatagagaa aattgaggcc attcttaagg aacttgaaaa gccagcaccc tgatgcgacc
     5881 tcgttttagt ctacgtttat ctgtctttac ttaatgtcct ttgttacagg ccagaaagca
     5941 taactggcct gaatattctc tctgggccca ctgttccact tgtatcgtcg gtctgataat
     6001 cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt
     6061 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     6121 tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     6181 gaccatggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     6241 tatcgtcggt ctgattatta gtctggaacc acggtcccac tcgtatcgtc ggtctgatta
     6301 ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacga
     6361 tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg
     6421 atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattgaca
     6481 tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat
     6541 tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg
     6601 gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc
     6661 gacgagctcg cgagctcgga catgaggttg ccccgtattc agtgtcgctg atttgtattg
     6721 tctgaagttg tttttacgtt aagttgatgc agatcaatta atacgatacc tgcgtcataa
     6781 ttgattattt gacgtggttt gatggcctcc acgcacgttg tgatatgtag atgataatca
     6841 ttatcacttt acgggtcctt tccggtgatc cgacaggtta cggggcggcg acctcgcggg
     6901 ttttcgctat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt cgtcataact
     6961 taatgttttt atttaaaata ccctctgaaa agaaaggaaa cgacaggtgc tgaaagcgag
     7021 ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac aatggaagtc
     7081 cgagctcatc gctaataact tcgtatagca tacattatac gaagttatat tcgatgcggc
     7141 cgcaaggggt tcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     7201 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccaca cagatgcgta
     7261 aggagaaaat accgcatcag gcgccattcg ccattcagct gcgcaactgt tgggaagggc
     7321 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7381 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7441 aattgtaata cgactcacta tagggcgaat tcgagctcgg tacccgggga tcctctagag
     7501 tcgacctgca ggcatgcaag cttgagtatt ctatagtctc acctaaatag cttggcgtaa
     7561 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7621 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7681 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7741 tgaatcggcc aacgcgaacc ccttgcggcc gcccgggccg tcgaccaatt ctcatgtttg
     7801 acagcttatc atcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     7861 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     7921 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     7981 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     8041 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     8101 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     8161 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     8221 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     8281 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     8341 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     8401 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     8461 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     8521 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     8581 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     8641 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     8701 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     8761 ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca gagaaagcgc
     8821 ggatctggga agtgacggac agaacggtca ggacctggat tggggaggcg gttgccgccg
     8881 ctgctgctga cggtgtgacg ttctctgttc cggtcacacc acatacgttc cgccattcct
     8941 atgcgatgca catgctgtat gccggtatac cgctgaaagt tctgcaaagc ctgatgggac
     9001 ataagtccat cagttcaacg gaagtctaca cgaaggtttt tgcgctggat gtggctgccc
     9061 ggcaccgggt gcagtttgcg atgccggagt ctgatgcggt tgcgatgctg aaacaattat
     9121 cctgagaata aatgccttgg cctttatatg gaaatgtgga actgagtgga tatgctgttt
     9181 ttgtctgtta aacagagaag ctggctgtta tccactgaga agcgaacgaa acagtcggga
     9241 aaatctccca ttatcgtaga gatccgcatt attaatctca ggagcctgtg tagcgtttat
     9301 aggaagtagt gttctgtcat gatgcctgca agcggtaacg aaaacgattt gaatatgcct
     9361 tcaggaacaa tagaaatctt cgtgcggtgt tacgttgaag tggagcggat tatgtcagca
     9421 atggacagaa caacctaatg aacacagaac catgatgtgg tctgtccttt tacagccagt
     9481 agtgctcgcc gcagtcgagc gacagggcga agcccgagcg ccagaaggcc gccagagagg
     9541 ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct
     9601 gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt
     9661 agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc
     9721 aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg
     9781 ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg
     9841 cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg
     9901 ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc
     9961 cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg
    10021 cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc
    10081 ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg
    10141 caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg
    10201 cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt
    10261 gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac
    10321 tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc
    10381 accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg
    10441 aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac
    10501 gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac
    10561 tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt
    10621 gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctt
    10681 gtctcgctgg ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg
    10741 gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc
    10801 gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc
    10861 gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc
    10921 tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc
    10981 tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc
    11041 aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg
    11101 gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc
    11161 gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt
    11221 ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg
    11281 cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg
    11341 acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg
    11401 ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa
    11461 ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg
    11521 atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc
    11581 tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc
    11641 gatcgctgat cgtggaaacg ataggcctat gccatgcggg tcaaggcgac ttccggcaag
    11701 ctatacgcgc cctaggagtg cggttggaac gttggcccag ccagatactc ccgatcacga
    11761 gcaggacgcc gatgatttga agcgcactca gcgtctgatc gcgcccaggc gtacatgctc
    11821 gcctgcgggg tctggacttc ccagcccagg tcacgcagcg ccgacagtag tgcgtcgcgg
    11881 cgttcctcga aaatgcgcgc tcctacccgg cccacctcgt cgggcagccc cagcgcgtgc
    11941 gccgccgccc gctgaatgcc cagatatggg tgaaaatcaa tcgccccctt gacccgcgcc
    12001 agcgccgcca ccgcgcccgc gtcccccgcc gcgaagccca cccggaagcc gccgaggtgg
    12061 tgcgtcttgg acagcgagtg cagttccacc acgccttcca tgcccgcttc cagtgccgag
    12121 ggggcgcggt agtcgccgaa ggtcagttcg gcatacggat ggtcatgcac cagcagcgtg
    12181 ccccgttcgc ggcaccacgc ggcggcttcg cggaaaaact cggcgctggc aatggccgag
    12241 gtcgggttgt tcgggtaatt gaggagcagc acgcgcggac gcacgttcgc tggcacccgg
    12301 ctgaggtccg gcagaaagcc gcgctccggc agcagcggca gcgtcaccac gttcagcccc
    12361 gccaccgccg ccgcgcccca gtacggcgga tagcaggggt cggggagcag tagcgtgtcg
    12421 ccggggtcgg tcacggcgag cagcaggtgc gccagccctt cttgcgctcc gatcagcggc
    12481 agcacctcgg tgtcggcatt gagccgcacg ccaaagcgcc gggcgaggta cgcggcggcg
    12541 gcgtcgcgca gaggctgggt gtcggaaaat agcgggtagc ggtaggtcgc cgggtcgtgg
    12601 gtggcggccc gcaattcgtc cagcacgctg agcggcgggt gctggtcgct ggagccgatg
    12661 ctgaggtcgg tgacgttcag gccccggccc cgcgcccgca tcttggcggc gtccatcagg
    12721 gaaaagacac tgccgggaac cgaggaagca cgctgagaag cccacatggc ccgagtgtaa
    12781 gccgtactct gaagaggttc tagggataac agggtaatgg ggtgggcgaa gaactccagc
    12841 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
    12901 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
    12961 tggtcggtca tttcgaaccc cagagtccca ctcagaagaa ctcgtcaaga aggcgataga
    13021 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
    13081 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
    13141 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
    13201 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatccgcg
    13261 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
    13321 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
    13381 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
    13441 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
    13501 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
    13561 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttggagt tcattcaggg
    13621 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
    13681 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
    13741 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
    13801 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
    13861 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
    13921 ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc
    13981 aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
    14041 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt
    14101 agcagccctt gcgccctgag tgcttgcggc agcgtgaagc tttgacagct agctcagtcc
    14161 taggtataat gctagctcac agaattccat actagagaaa gaggagaaat actagatggt
    14221 gaaaagaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    14281 tcattaatgc agctggcacg acaggtttcc cgacttaatc gccttgcagc acatccccct
    14341 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    14401 agcctgaatg gcgaatggcg ctttgcctgg tttccagcac cagaagcggt gccggaaagc
    14461 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    14521 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
    14581 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    14641 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
    14701 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
    14761 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
    14821 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
    14881 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
    14941 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
    15001 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
    15061 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
    15121 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    15181 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    15241 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
    15301 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    15361 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
    15421 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
    15481 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    15541 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    15601 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
    15661 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
    15721 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    15781 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    15841 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
    15901 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
    15961 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
    16021 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
    16081 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    16141 gggcaaacca tcgaagtgac cagcgaatac ctgttccgcc atagcgataa cgagctcctg
    16201 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
    16261 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
    16321 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
    16381 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
    16441 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
    16501 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
    16561 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    16621 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
    16681 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
    16741 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
    16801 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
    16861 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
    16921 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    16981 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccata cgtcttcccg
    17041 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    17101 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
    17161 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    17221 attggtggcg acgactcctg gagcccgtca gtatcggcgg aatttcaact gagcgccggt
    17281 cgctaccatt accagttggt ctggtgtcaa aaataactag tgtagatcgc tactagagcc
    17341 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
    17401 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
    17461 gtttatagaa aagacactgc cgggaaccga ggaagcacgc tgagaagccc acatggcccg
    17521 agtgtaagcc gtactctgaa gaggttcaca ccgaaagcgg gcacccaggg aagctcccgg
    17581 gtgcccgccc tgctgactgg accgtgctca gccccccacg tgcacctgtg ggcggcgtcc
    17641 ggggtcgcgc tcggccaccc gcagaatttc gtgggtgagc ggcggaatgt cgccctcccc
    17701 ggcgagcagg aagcgcaggg cgttggcggc ggggcctttt tcgctccact cgaagtagat
    17761 gtggggcggc gcgccggtca ggtcacggac gtagagcagc acggcggcga tggtgttggg
    17821 cacgctgttg ccggtggcgc gcaggatgcg gtgggggccg acctgcacgc cctccaggta
    17881 caccgtcgat ttgaaatccg aggcgtcgag caccgccact tccagaaaca gggccacctc
    17941 gcccgcgctg aggtggttgt cgagccgcac ttccagcgcc ttttcctggt actcggcggc
    18001 gtcgcccgcg ttcaggtggt tggcgataaa acgcaccggc agcccgtggg cggccacgtc
    18061 ccgcaccatg tccagccccg cttcatcgaa gtggacattc tgcacccgca actcggtgct
    18121 gcgcccgatg cgcgaccaga tgctcacgac cagaatcccg gcgataaagc acagggcaat
    18181 ccacaggccc tcgggccgct ccttaacggt cacgaaggcg gtgtagacga aaatcaggct
    18241 gaccagcccg aacgccagcg ccgcctgggg gtgtttgcgc cgcagctcgg tcagaaagac
    18301 ggcgatggct gccgaggtca tcagtgccag cacgccggtc gcgtaggccc cggcctgggc
    18361 gtccacgttc gcctgaaaca ccagcgtgac cagcgcggcg atgccggtaa acaccagcac
    18421 cagcgggcgg gtggcgcgcg cccagtccgg cgccatgccg tagcgcggca ggtaacgcgg
    18481 cacgatgttg agcagccccg ccatggccga ggcgcccgca aaccacagga tgaaaatggt
    18541 gctcacgatc ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
    18601 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
    18661 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
    18721 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
    18781 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
    18841 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag
    18901 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg
    18961 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca
    19021 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc
    19081 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc
    19141 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt
    19201 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc
    19261 atcaagaaga ggcacttcga gctgtaagta catcaccgac gagcaaggca agacgatc
//
