LOCUS       sequence-38893-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EN2K
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(632..649)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cacctaccta ccccagcctt tactcttcct accaccaggg atgcctggtg aacncgactg
       61 ggaaccttcc aatgtggagc aaccagacct ggaacaattc aacctggagc aaccagaccc
      121 agaacatcca gtcctggagc aaccactcct ggaacactca gacctggtgc acccaatcct
      181 ggaacaatca ggcctggaac agtcccttct ataactgtgg agaggaatct ctgcagtcct
      241 gcatgcagtt ccagccaaat tctcctgcca gtgacttgga ggctgccttg gaagctgctg
      301 gggaaggcct taatgtaata cagcagacca ctaggtattt tagtactcca caaaccatgg
      361 atttattcct aaactactcc atgaacatgc aacctgaaga cgtgtgaaga tgaatcgaat
      421 tcccgcggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
      481 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
      541 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      601 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      661 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      721 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaa
//
