LOCUS       sequence-388942-        7036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Myc-tagged Rabex-5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Mattera R, Tsai YC, Weissman AM, Bonifacino JS
  TITLE     The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin 
            (Ub) and functions as a Ub ligase through an atypical Ub-interacting
            motif and a zinc finger domain.
  JOURNAL   J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 
            10.1074/jbc.M509939200. Epub 2006 Jan 5.
  PUBMED    16407276
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M509939200"; journalName: "J Biol Chem"; date: 
            "2006-03-10- 10"; volume: "281"; issue: "10"; pages: "6874-83"
COMMENT     Sequence Label: pCI-neo-Myc-Rabex-5
FEATURES             Location/Qualifiers
     source          1..7036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..418
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        61..252
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(84..104)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      269..404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             469..1263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(523..542)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1133..1152
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1327..1375
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1416..1435)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1535..1557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1595..1613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1681..1785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1786..1854,1855..2646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2004..2023)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2817..3405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3306..3325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3616..3995
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3996..4207
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4158..4178
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4204..4228
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4368..4500
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4483..4502
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4545..4563
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4619..4648
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6218..6339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6229..6248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6283..6302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6525..6980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6612..6631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6822..6843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
       61 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      421 gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa
      481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     1021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     1321 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     1381 atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1441 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1501 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1561 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     1621 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1681 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1741 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatcttc
     3481 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt
     3541 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     3601 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
     3661 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3721 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3781 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3841 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     3901 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     3961 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     4021 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4081 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactgcg
     4141 atcgcccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4201 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
     4261 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
     4321 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
     4381 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
     4441 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
     4501 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     4561 aggctagcct cgagaattca cgcgtggtac ctctagagtc gaccccatgg cccatatgga
     4621 acaaaaactc atctcagaag aggatctggg tggcggccgc tctagcccta tgagccttaa
     4681 gtctgaacgc cgaggaattc atgtggatca gtcggagctc ctatgcaaga aaggatgtgg
     4741 ttactacggc aaccctgctt ggcagggttt ctgctccaag tgctggagag aggagtacca
     4801 caaagccagg cagaagcaga ttcaggagga ctgggaactg gcagagcgac tgcagcggga
     4861 ggaagaagag gcctttgcca gcagtcagag cagccaaggt gcccaatccc tcacattttc
     4921 caagtttgaa gagaagaaaa ccaatgagaa gacccgcaag gttaccacag tgaagaaatt
     4981 cttcagtgct tcatccaggg ttggagcaaa gaaggcagaa attcaggaag caaaagctcc
     5041 cagtccttcc ataaaccggc aaaccagcat tgaaacggat agagtgtcta aagagttcat
     5101 agaatttctc aagaccttcc acaagacagg ccaagaaatc tataagcaga ccaagctgtt
     5161 tttggaagca atgcattaca aaagggactt aagtattgag gagcagtcag agtgtactca
     5221 ggatttttac cagaatgtgg ctgaaagaat gcagactcgt ggaaaagtgc ctccagaaag
     5281 agttgagaag ataatggatc agattgaaaa gtacatcatg actcgtctct ataaatatgt
     5341 gttctgtcca gaaactactg atgatgagaa gaaagatctt gctattcaga agaggatcag
     5401 agccctgcac tgggttacac ctcagatgct ttgtgtccct gttaatgaag aaattccaga
     5461 agtgtccgat atggtggtga aagcaattac agatatcatt gagatggatt cgaaacgtgt
     5521 gccccgggat aagttggcct gcatcaccaa gtgcagcaag cacatcttca atgccatcaa
     5581 gatcaccaag aatgagccgg cttcagccga cgacttctta cccacactca tctacattgt
     5641 cttgaagggc aaccccccac gccttcagtc caacatccag tacatcaccc gcttctgcaa
     5701 cccaagccga ctgatgacgg gtgaggatgg ctactatttc accaatctgt gctgtgccgt
     5761 ggctttcatt gagaaattag atgctcagtc tttgaattta agtcaggaag attttgatcg
     5821 atacatgtct ggccagactt ctcccaggaa gcaggaatct gagaattggt ctcctgatgc
     5881 gtgcttaggt gtgaagcaga tgtataagaa tttggacctc ctgtctcagt tgaatgaacg
     5941 acaggaaagg attatgaatg aagccaagaa acttgagaaa gacctcatag attggacgga
     6001 tggaatcgca aaagaggttc aagacattgt tgagaaatac ccacttgaaa ttagaccccc
     6061 aaatcagtct gtagcagcta ttgactctga aaatgttgaa aatgataaac ttcctccacc
     6121 gttgcaacct caagtttatg caggataggc tcagactacc cgggggaatc actagccgct
     6181 tccctttagt gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga
     6241 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6301 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6361 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     6421 aaatgtggta aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca
     6481 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     6541 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6601 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6661 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6721 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6781 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6841 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6901 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6961 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7021 tcacaccgca tacgcg
//
