LOCUS       sequence-388943-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of fluorescently-tagged marker for autophagic 
            vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Dou D, Smith EM, Evans CS, Boecker CA, Holzbaur ELF
  TITLE     Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and 
            ARF6 GTPase disrupts the axonal transport of autophagosomes.
  JOURNAL   Cell Rep. 2023 May 1;42(5):112448. doi: 
            10.1016/j.celrep.2023.112448.
  PUBMED    37133994
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-05-1"; volume: "42(5):112448. doi"; pages: " 
            10.1016/j.celrep.2023.112448"
COMMENT     Sequence Label: CMV mScarlet-LC3B
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..534
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
ORIGIN
        1 atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc
       61 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctag accgtccgag
      121 aagaccttca aacagcgccg gagcttcgaa caaagagtgg aagatgtccg gctcatccgg
      181 gagcagcacc ccaccaagat cccagtgatt atagagcgat acaagggtga gaagcagctg
      241 cccgtcctgg acaagaccaa gttccttgta cctgatcacg tgaatatgag cgaactcatc
      301 aagataatta gaaggcgcct gcagctcaat gctaaccaag ccttcttcct cctggtgaat
      361 gggcacagca tggtgagtgt gtccacaccc atctctgaag tgtacgagag cgagagagat
      421 gaagacggct tcctgtacat ggtctatgcc tcccaggaga cgttcgggac agcactggct
      481 gttacataca tgtcagctct gaaggcaaca gcaacaggaa gagagccatg cttgtgacag
      541 acatacagcc acttccaact aaagcaagcc tctgctccct gctacctgca tggagcccac
      601 tgtgacactc agaccatccc cggtcactca ctcactcgtg tctgagaatc tcagtgagag
      661 ctgcctctgt cacggacgga agccaacaca gccacctctc gacctggctc cccacagcac
      721 ccaccctccc tgcatgcagc tgtccctgct aacccccaat gttatgttac actgtgtaag
      781 tccccactgc tgccgtgtgt gggttgtgta cgtcgtcatg tccctggttt ataactatgg
      841 tgcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
      901 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      961 ccccctgaac ctgaaacata aaatgaatgc aatgttgtgt aaccgaat
//
