LOCUS       sequence-38897-e       14687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Tang Y, Wang F, Zhao J, Xie K, Hong Y, Liu Y
  TITLE     Virus-based microRNA expression for gene functional analysis in 
            plants.
  JOURNAL   Plant Physiol. 2010 Jun;153(2):632-41. Epub 2010 Apr 13.
  PUBMED    20388670
REFERENCE   2  (bases 1 to 14687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2010-06"; volume: "153(2)"; pages: "632-41. Epub 
            2010 Apr 13"
COMMENT     Sequence Label: pCVA-amiR-PDS
FEATURES             Location/Qualifiers
     source          1..14687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(450..467)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(450..466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(459..481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        843..1188
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1205..1219,1410..3227)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRS
                     VAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          1220..1409
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             3234..3251
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3286..3538
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3560..3584
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4884..5513
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5947..7014
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7080..7274
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7510..7532)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7618..7758
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7673..7692
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7773..7790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7944..8532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8024..8043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8619..9413)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9838..9862
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9940..10114
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10154..11179)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11247..11924)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    12115..12136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12151..12156,12157..12174,12175..12181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12189..12205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12194..12216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12213..12229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12213..12229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14199..14215
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14199..14215
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14655..14671)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14656..14672)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgctagccgc ttaggcattt cgaagtaaag cataaattga aatcacatgt ctttatatag
       61 gcccacagcg cactcagtta gccttaatag ctaaccttgt caggcggcat gtgattggtt
      121 agatttcatt taatgcgtgg tcccacgcaa gacaccaaaa aaatcgcggc catccggtaa
      181 tattattgcg gatggccgct tttgcccctg gtgttcccca gaggctttta gtatatattg
      241 gacaccaaga caccaaaaac aataggtttt agagagagct ctcctggcgt cctcccctgg
      301 agtcctaaat attatttaca aaaatgccac gaaaccctaa atcgtttcgt ttagcagccc
      361 gaaatatatt cttaactcac ctaaggtgcg acatacccaa agatgaagcg ggggatccac
      421 tagagcggcc gccagtcgac aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
      481 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      541 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      601 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc
      661 atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag
      721 ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
      781 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      841 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      901 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      961 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
     1021 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     1081 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1141 caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt
     1201 gaccatggta gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac
     1261 ccttttctct ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat
     1321 tgattggtta tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg
     1381 tgtgtctatg atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc
     1441 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact
     1501 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc
     1561 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct
     1621 ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt
     1681 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc
     1741 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg
     1801 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg
     1861 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg
     1921 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
     1981 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg
     2041 ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac
     2101 aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt
     2161 atctctatga actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc
     2221 gcgtcggcat ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt
     2281 tctactttac tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata
     2341 acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta
     2401 cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg
     2461 tgattgatga aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg
     2521 gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc
     2581 acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt
     2641 ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac
     2701 tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt
     2761 tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt
     2821 attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag
     2881 aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg
     2941 atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg
     3001 catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg
     3061 tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga
     3121 aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct
     3181 ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc
     3241 accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt
     3301 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3361 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3421 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3481 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3541 gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     3601 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     3661 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     3721 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     3781 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     3841 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     3901 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     3961 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     4021 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     4081 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     4141 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     4201 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     4261 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4321 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4381 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4441 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4501 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4561 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     4621 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     4681 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     4741 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     4801 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     4861 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     4921 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     4981 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     5041 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     5101 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     5161 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     5221 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     5281 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5341 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5401 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5461 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5521 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     5581 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     5641 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     5701 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     5761 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     5821 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     5881 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     5941 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     6001 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     6061 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     6121 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     6181 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     6241 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     6301 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     6361 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     6421 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     6481 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     6541 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     6601 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     6661 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     6721 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     6781 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     6841 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     6901 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     6961 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     7021 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     7081 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     7141 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     7201 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     7261 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     7321 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     7381 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     7441 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     7501 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     7561 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     7621 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     7681 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     7741 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8341 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8581 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     8641 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     8701 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     8761 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     8821 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     8881 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     8941 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     9001 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     9061 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     9121 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     9181 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     9241 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     9301 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     9361 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     9421 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     9481 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     9541 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     9601 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     9661 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     9721 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     9781 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     9841 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     9901 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
     9961 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
    10021 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
    10081 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg
    10141 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
    10201 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
    10261 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
    10321 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
    10381 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
    10441 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
    10501 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
    10561 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
    10621 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
    10681 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
    10741 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
    10801 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
    10861 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
    10921 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
    10981 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
    11041 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
    11101 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
    11161 tgagttcagg ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat
    11221 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
    11281 tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
    11341 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    11401 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
    11461 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    11521 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    11581 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
    11641 agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg
    11701 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    11761 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    11821 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    11881 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    11941 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg
    12001 caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    12061 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    12121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    12181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga
    12241 attcgaggtc gacatagaac gccacggggc atcccgctta ggcatttcga agtaaagcat
    12301 aaattgaaat cacatgtctt tatataggcc cacagcgcac tcagttagcc ttaatagcta
    12361 accttgtcag gcggcatgtg attggttaga tttcatttaa tgcgtggtcc cacgcaagac
    12421 accaaaaaaa tcgcggccat ccggtaatat tattgcggat ggccgctttt gcccctggtg
    12481 ttccccagag gcttttagta tatattggac accaagacac caaaaacaat aggttttaga
    12541 gagagctctc ctggcgtcct cccctggagt cctaaatatt atttacaaaa atgccacgaa
    12601 accctaaatc gtttcgttta gcagcccgaa atatattctt aactcaccta aggtgcgaca
    12661 tacccaaaga tgaagctctt cagatgcttc aaaccctgtc gtggtcagtc gtcaaaccca
    12721 catacatcag agtcgcaaga gaggaacatt cagacggttc cccatttaca cactgtctca
    12781 tccaactatc aggaaagtcg aacatcaagg atgctagatt tttcgacatc actcacccca
    12841 gaaggtctgc caattttcac ccaaacattc aggcagccaa agacaccaat gccgtcaaga
    12901 attacatcac caaagatggt gattattgtg aatccgggca gtacaaggtg tctgggggta
    12961 caaaggcaaa taaagacgac gtctaccaca acgccgtcaa tgcgggatgt gtggaagagc
    13021 gtctcgcaat tataagggct ggagttccaa agacgttcat tgttagttat cataatgtta
    13081 gagctaacat agagcgactc tttactaagg ctccggaacc atgggctcct ccgtttcaac
    13141 tctcctcctt tactaacgtc ccggacgaga tgagttcatg ggcagatgac tattttggtc
    13201 ggagtgccgc tgcgcgggcg gaaagaccta ttagtatcat agttgaaggt gattcacgaa
    13261 ccggcaagac catgtgggcg cgtgctttag gaccacataa ttatttgagt gggcacctcg
    13321 actttaattc aaaggtcttt tcaaataatg cggagtataa cgtcattgat gacatagctc
    13381 cgcattatct aaagctaaag cactggaaag agcttattgg ggcccaaagg gactggcaat
    13441 caaactgtaa gtacggcaag ccagttcaaa ttaaaggtgg cataccctca atcgtgctgt
    13501 gcaatccagg agaggggagc agttatataa gtttcctcaa caaagaggaa aatgcatcac
    13561 taagagcgtg gactaccaaa aatgcaaaat tcatcactct tgaagccccc ctctatcaaa
    13621 gcacagcaca agattgctaa aaggagagct gtacgccgaa gacgcattga tttgaactgc
    13681 gggtgttcta tcttcctcca catcaattgc gcagataatg gattcacgca ccggggagag
    13741 catcactgta gctcaggcag agaattccgt ttttatctgg gaggttccaa atcccctata
    13801 tttcaggata caacgcgtag aggacccgtt gtacaccaga accaggatct accacatcca
    13861 agtccggttc aaccacaacc tacggaaagc attggatctc cgcaaagcct acttcaactt
    13921 ccaagtctgg acgacttcga tgagagcttc tgggccgaca tatttaagta gattcaaatg
    13981 ccttgtaatg tctcatttag ataacctagg tgttattggt attaatcatg taattagagc
    14041 tgttcgtttc gcaacggaca gatcctatgt aactcatgtt cacgagaatc atgtaataaa
    14101 tttcaaaatt tattaatttg ttatcgaatc gtaaaaatag atccgaattt ttaatgtcgc
    14161 atacacagga ttaggctagc gagctcagat ctggatcctc tagaactagt ggatcccccc
    14221 atggcgatgc cttaaataaa gataaaccca aaatgttaat tttaccagaa ctatatatac
    14281 gaaggcagca tatatgtcac ttagtggatc aagcatgttt ttgtgcagga aagattaatc
    14341 aagaaaattg gaatacaaaa gagagagccc caatcagtct atgttgatca aagagaatca
    14401 atgatccaat ttgtctaccg catcattcat tcatttaacg agtctagttt gaattttggc
    14461 gactcggtat ttggatgaat gagtcggaag ctaattgaat catatcacga cctgtgaaca
    14521 acattgactg attggtgctc tacatatata ttcctaaaac atcaattcaa aacagcgagt
    14581 attaagtgta tgaacatgtg taatatgcgt ccgagcgtgt gtttggggct gcaggaattc
    14641 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtac
//
