LOCUS       sequence-388972-        9959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by 
            CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, 
            and mCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Kong X, Zhang H, Li G, Wang Z, Kong X, Wang L, Xue M, Zhang W, Wang 
            Y, Lin J, Zhou J, Shen X, Wei Y, Zhong N, Bai W, Yuan Y, Shi L, Zhou
            Y, Yang H
  TITLE     Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and
            expanded target range for genome editing.
  JOURNAL   Nat Commun. 2023 Apr 11;14(1):2046. doi: 10.1038/s41467-023-37829-7.
  PUBMED    37041195
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-11"; volume: "14(1):2046. doi"; pages: " 
            10.1038/s41467-023-37829-7"
COMMENT     Sequence Label: miniCRISPRoff-v1
FEATURES             Location/Qualifiers
     source          1..9959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          961..1188
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1200..1209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3108..3128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4428..4475
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4479..4505
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4485..4511
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4524..4544
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4551..4571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4611..5306
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             5352..5537
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     primer_bind     complement(5565..5582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5571..5778
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5779..6027
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5779..5799
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5950..5969
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     repeat_region   6556..6590
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        6648..6951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6952..7155
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7106..7126
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7184..7894
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7329..7347)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    7911..8135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8162..8266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8267..8335,8336..9127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8485..8504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9298..9886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9787..9806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccrggggggg sggggsgggg sggggggsgg
      841 ggsggggsgr ggsggrgrgg kgsggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1081 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1141 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1201 ccaccatgga catgaaccat gaccaggaat ttgacccccc aaaggtttac ccacctgtgc
     1261 cagctgagaa gaggaagccc atccgcgtgc tgtctctctt tgatgggatt gctacagggc
     1321 tcctggtgct gaaggacctg ggcatccaag tggaccgcta cattgcctcc gaggtgtgtg
     1381 aggactccat cacggtgggc atggtgcggc accagggaaa gatcatgtac gtcggggacg
     1441 tccgcagcgt cacacagaag catatccagg agtggggccc attcgacctg gtgattggag
     1501 gcagtccctg caatgacctc tccattgtca accctgcccg caagggactt tatgagggta
     1561 ctggccgcct cttctttgag ttctaccgcc tcctgcatga tgcgcggccc aaggagggag
     1621 atgatcgccc cttcttctgg ctctttgaga atgtggtggc catgggcgtt agtgacaaga
     1681 gggacatctc gcgatttctt gagtctaacc ccgtgatgat tgacgccaaa gaagtgtctg
     1741 ctgcacacag ggcccgttac ttctggggta accttcctgg catgaacagg cctttggcat
     1801 ccactgtgaa tgataagctg gagctgcaag agtgtctgga gcacggcaga atagccaagt
     1861 tcagcaaagt gaggaccatt accaccaggt caaactctat aaagcagggc aaagaccagc
     1921 atttccccgt tttcatgaac gagaaggagg acatcctgtg gtgcactgaa atggaaaggg
     1981 tgtttggctt ccccgtccac tacacagacg tctccaacat gagccgcttg gcgaggcaga
     2041 gactgctggg ccgatcgtgg agcgtgccgg tcatccgcca cctcttcgct ccgctgaagg
     2101 aatattttgc ttgtgtgtct agcggcaata gtaacgctaa cagccgcggg ccgagcttca
     2161 gcagcggcct ggtgccgtta agcttgcgcg gcagccatat gggccctatg gagatataca
     2221 agacagtgtc tgcatggaag agacagccag tgcgggtact gagcctcttc agaaacatcg
     2281 acaaggtact aaagagtttg ggcttcttgg aaagcggttc tggttctggg ggaggaacgc
     2341 tgaagtacgt ggaagatgtc acaaatgtcg tgaggagaga cgtggagaaa tggggcccct
     2401 ttgacctggt gtacggctcg acgcagcccc taggcagctc ttgtgatcgc tgtcccggct
     2461 ggtacatgtt ccagttccac cggatcctgc agtatgcgct gcctcgccag gagagtcagc
     2521 ggcccttctt ctggatattc atggacaatc tgctgctgac tgaggatgac caagagacaa
     2581 ctacccgctt ccttcagaca gaggctgtga ccctccagga tgtccgtggc agagactacc
     2641 agaatgctat gcgggtgtgg agcaacattc cagggctgaa gagcaagcat gcgcccctga
     2701 ccccaaagga agaagagtat ctgcaagccc aagtcagaag caggagcaag ctggacgccc
     2761 cgaaagttga cctcctggtg aagaactgcc ttctcccgct gagagagtac ttcaagtatt
     2821 tttctcaaaa ctcacttcct cttggagggc cgagctctgg cgcaccccca ccaagtggag
     2881 gatctcctgc cgggtcccca acatctactg aagaaggcac cagcgaatcc gcaacgcccg
     2941 agtcaggccc tggtacctcc acagaaccat ctgaaggtag tgcgcctggt tccccagctg
     3001 gaagccctac ttccaccgaa gaaggcacgt caaccgaacc aagtgaagga tctgcccctg
     3061 ggaccagcac tgaaccatct gagcgaggaa gcggaagcgg atccatgcct aagaagaaga
     3121 gaaaggtgat gggaaagggc gtgctggcca aggtgatgaa atacgagctg agatacctgg
     3181 atggttgtgg cgacttcagc aatatgcagg agcaggtgtg ggccctgcag cggcagacac
     3241 gggaaatcct gaacagatcc atccaaatcg ccttccaatg gcgctgcgcc aacagcgagc
     3301 accacagaaa gaccggcgag tacctggacc tgaaaacgga aaccggctac aagagactgg
     3361 atggccacat ctacaactgc ctgaagggcc agtacgagga catggccaca tctaacctga
     3421 acgccaccat ccagaaggct tggaagaagt ataactccag caagaaggaa atcctgaggg
     3481 gcagcatgag catccccagc tacaagatga accagcctct gcgactggac aagaataccg
     3541 tgaaactgtc tgagggcgag cggaacccaa tcgtgaccct gacactgttt agcgacaagt
     3601 tcaagcgggc ccagggcgtg tccaacgtga agtttagcat gcctctgcac gacggcaccc
     3661 agagagccat cttcgccaac ctgatgaacg gcacctacca gctgggagag tgccagctgg
     3721 tgtacaaacg gcctaagtgg ttcctgttcg tgacatacaa gttccccccc gtggaacatc
     3781 ctctcgatcc tgacaagatt ctgggcgtcg ccatgggcga ggcctgcgcg ctttatgcct
     3841 ctacattcgg cgagcacggc tacctgaaga tcgatggagg cgagattaca aagtacgcca
     3901 agaagatgga agctagaatc cggagcatgc agaagcaggc tgctcactgt ggcgaaggca
     3961 gaatcgggca cggcaccaaa acaagagtgt ctgtggtgta ccaggccaag gacaaggtgg
     4021 ccagattcag agataccatc aaccacagat actctaaggc cctgatcgac tacgccctga
     4081 agaaccagtg tggcaccatc cagatggaag atctgaccgg catcaaggaa gatacaggat
     4141 ttccaaagtt cctgagacat tggacctact acgacctgca gagcaagatc gaggctaagg
     4201 ccgccgagca cggcatccaa gttgtcaaga tcaaccctag acacaccagc cagcgctgca
     4261 gcagatgtgg acacatcgac aaagccaata gaaccagcca agctgatttc tgctgcacca
     4321 agtgcggctt cagcgccaat gccgccttta atgccagcca gaacatcagc atcagaaaca
     4381 tcgacaagat tatcgccaag gctatcggcg ccaaccggaa gcagaccaaa aggccggcgg
     4441 ccacgaagaa ggccggccag gcaaagaaga agaaggccta tccctatgac gtgcccgatt
     4501 atgccagcct gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa
     4561 agcggaaagt ggacggcatt ggtagtggga gcaacggcag cagcggatcc agcgagctga
     4621 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca
     4681 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
     4741 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg
     4801 gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc
     4861 ctgagggctt cacatgggag agagtcacca catacgagga cgggggcgtg ctgaccgcta
     4921 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
     4981 acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg
     5041 agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg
     5101 tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta
     5161 agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg
     5221 ccaacaacga aacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc
     5281 ctagcaaact ggggcacaag cttaatggcg gtggcggagg gatggatgct aagtcactaa
     5341 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg
     5401 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact
     5461 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg
     5521 agaagggaga agagccctag gaattcctag agctcgctga tcagcctcga ctgtgccttc
     5581 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5641 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5701 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
     5761 tagcaggcat gctggggaga gggcctattt cccatgattc cttcatattt gcatatacga
     5821 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     5881 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     5941 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     6001 tatatcttgt ggaaaggacg aaacaccgag ggccgacttc ccggcccaaa atcgagacag
     6061 tagccgtaaa acgttgagtt tcagcgtggg cgacacactc gaaaaggtta agatatgcac
     6121 atagtaatcc gtgcatgagc cgcgaaagcg gcttgaaggt tgtgcagtgc caggtgaaat
     6181 tttatttttt tgcggccgcg tcgacggcgc gccctagacg aagcagctcc agcctacaca
     6241 atcgctcaag acgtgtaatg cttttattat atattagtca cgatatctat aacaagaaaa
     6301 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     6361 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     6421 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     6481 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     6541 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaggcaac
     6601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctctagacgt tacataactt
     6661 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     6721 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     6781 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     6841 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     6901 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     6961 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     7021 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     7081 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     7141 tatataagca gagctggttt agtgaaccgt cagatccgct agcatggtga gcaagggcga
     7201 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     7261 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     7321 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     7381 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     7441 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgatga
     7501 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     7561 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
     7621 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     7681 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     7741 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     7801 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     7861 ctccaccggc ggcatggacg agctgtacaa gtaactcttg gcccggatcc ctgtgccttc
     7921 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7981 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     8041 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaaaacaa
     8101 tagcaggcat gctggggatg cggtgggctc tatggggatc cgcacttttc ggggaaatgt
     8161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8281 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8341 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8401 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8461 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8521 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8581 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8641 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8701 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8761 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8821 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8881 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8941 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9001 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9061 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9121 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9421 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9721 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9901 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgt
//
