LOCUS       sequence-388973-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding a human codon-optimized dead enOsCas12f1 
            (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible 
            with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Kong X, Zhang H, Li G, Wang Z, Kong X, Wang L, Xue M, Zhang W, Wang 
            Y, Lin J, Zhou J, Shen X, Wei Y, Zhong N, Bai W, Yuan Y, Shi L, Zhou
            Y, Yang H
  TITLE     Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and
            expanded target range for genome editing.
  JOURNAL   Nat Commun. 2023 Apr 11;14(1):2046. doi: 10.1038/s41467-023-37829-7.
  PUBMED    37041195
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-11"; volume: "14(1):2046. doi"; pages: " 
            10.1038/s41467-023-37829-7"
COMMENT     Sequence Label: pCBh_3x
            Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mChe...
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          961..1188
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1200..1209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1209..1274
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1278..1298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2598..2645
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2649..2675
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2655..2681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             2727..2876
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3321..3692
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             3711..4280
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             4308..4334
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             4347..5054
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFD
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4489..4507)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5079..5096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5085..5292
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5293..5541
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5293..5313
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5464..5483
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     repeat_region   6070..6104
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        6162..6465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6466..6669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6620..6640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6698..7399
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    7400..7607
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        7634..7738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7739..7807,7808..8599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7957..7976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8770..9358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9259..9278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg sggggggsgg
      841 ggsggggsgr ggsggrgrgg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1081 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1141 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1201 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
     1261 acgatgacga taagatgcct aagaagaaga gaaaggtgat gggaaagggc gtgctggcca
     1321 aggtgatgaa atacgagctg agatacctgg atggttgtgg cgacttcagc aatatgcagg
     1381 agcaggtgtg ggccctgcag cggcagacac gggaaatcct gaacagatcc atccaaatcg
     1441 ccttccaatg gcgctgcgcc aacagcgagc accacagaaa gaccggcgag tacctggacc
     1501 tgaaaacgga aaccggctac aagagactgg atggccacat ctacaactgc ctgaagggcc
     1561 agtacgagga catggccaca tctaacctga acgccaccat ccagaaggct tggaagaagt
     1621 ataactccag caagaaggaa atcctgaggg gcagcatgag catccccagc tacaagatga
     1681 accagcctct gcgactggac aagaataccg tgaaactgtc tgagggcgag cggaacccaa
     1741 tcgtgaccct gacactgttt agcgacaagt tcaagcgggc ccagggcgtg tccaacgtga
     1801 agtttagcat gcctctgcac gacggcaccc agagagccat cttcgccaac ctgatgaacg
     1861 gcacctacca gctgggagag tgccagctgg tgtacaaacg gcctaagtgg ttcctgttcg
     1921 tgacatacaa gttccccccc gtggaacatc ctctcgatcc tgacaagatt ctgggcgtcg
     1981 ccatgggcga ggcctgcgcg ctttatgcct ctacattcgg cgagcacggc tacctgaaga
     2041 tcgatggagg cgagattaca aagtacgcca agaagatgga agctagaatc cggagcatgc
     2101 agaagcaggc tgctcactgt ggcgaaggca gaatcgggca cggcaccaaa acaagagtgt
     2161 ctgtggtgta ccaggccaag gacaaggtgg ccagattcag agataccatc aaccacagat
     2221 actctaaggc cctgatcgac tacgccctga agaaccagtg tggcaccatc cagatggaag
     2281 atctgaccgg catcaaggaa gatacaggat ttccaaagtt cctgagacat tggacctact
     2341 acgacctgca gagcaagatc gaggctaagg ccgccgagca cggcatccaa gttgtcaaga
     2401 tcaaccctag acacaccagc cagcgctgca gcagatgtgg acacatcgac aaagccaata
     2461 gaaccagcca agctgatttc tgctgcacca agtgcggctt cagcgccaat gccgccttta
     2521 atgccagcca gaacatcagc atcagaaaca tcgacaagat tatcgccaag gctatcggcg
     2581 ccaaccggaa gcagaccaaa aggccggcgg ccacgaagaa ggccggccag gcaaagaaga
     2641 agaaggccta tccctatgac gtgcccgatt atgccagcct gggcagcggc gacggcattg
     2701 gtagtgggag caacggcagc agcctcgatg ctttagacga ttttgactta gatatgcttg
     2761 gttcagacgc gttagacgac ttcgacctag acatgttagg ctcagatgca ttggacgact
     2821 tcgatttaga tatgttgggc tccgatgccc tagatgactt tgatctagat atgctaggta
     2881 gtggcggcag cggatcccag tatctgcccg acacagatga tagacaccga atcgaagaga
     2941 aacgcaagcg aacgtatgaa accttcaaat cgatcatgaa gaaatcgccc ttctcgggtc
     3001 cgaccgatcc caggccccca ccgagaagga ttgcggtccc gtcccgctcg tcggccagcg
     3061 tgccgaagcc tgcgccgcag ccctacccct tcacgtcgag cctgagcaca atcaattatg
     3121 acgagttccc gacgatggtg ttcccctcgg gacaaatctc acaagcctcg gcgctcgcac
     3181 cagcgcctcc ccaagtcctt ccgcaagcgc ctgccccagc gcctgcaccg gcaatggtgt
     3241 ccgccctcgc acaggcccct gcgcccgtcc ccgtgctcgc gcctggaccg ccccaggcgg
     3301 tcgctccacc ggctccgaag ccgacgcagg ccggagaggg aacactctcc gaagcacttc
     3361 ttcaactcca gtttgatgac gaggatcttg gagcactcct tggaaactcg acagaccctg
     3421 cggtgtttac cgacctcgcg tcagtagata actccgaatt tcagcagctt ttgaaccagg
     3481 gtatcccggt cgcgccacat acaacggagc ccatgttgat ggaatacccc gaagcaatca
     3541 cgagacttgt gacgggagcg cagcggcctc ccgatcccgc acccgcacct ttgggggcac
     3601 ctggcctccc taacggactt ttgagcggcg acgaggattt ctcctccatc gccgatatgg
     3661 atttctcagc cttgctgtca cagatttcca gcggctctgg cagcggcagc cgggattcca
     3721 gggaagggat gtttttgccg aagcctgagg ccggctccgc tattagtgac gtgtttgagg
     3781 gccgcgaggt gtgccagcca aaacgaatcc ggccatttca tcctccagga agtccatggg
     3841 ccaaccgccc actccccgcc agcctcgcac caacaccaac cggtccagta catgagccag
     3901 tcgggtcact gaccccggca ccagtccctc agccactgga tccagcgccc gcagtgactc
     3961 ccgaggccag tcacctgttg gaggatcccg atgaagagac gagccaggct gtcaaagccc
     4021 ttcgggagat ggccgatact gtgattcccc agaaggaaga ggctgcaatc tgtggccaaa
     4081 tggacctttc ccatccgccc ccaaggggcc atctggatga gctgacaacc acacttgagt
     4141 ccatgaccga ggatctgaac ctggactcac ccctgacccc ggaattgaac gagattctgg
     4201 ataccttcct gaacgacgag tgcctcttgc atgccatgca tatcagcaca ggactgtcca
     4261 tcttcgacac atctctgttt catatgggtg gaggctccgg ggaagatcct gctgccaaac
     4321 gcgttaaact agacatggga agcggagtga gcaagggcga ggaggataac atggccatca
     4381 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     4441 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     4501 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     4561 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     4621 ccgagggctt caagtgggag cgcgtgatga acttcgatga cggcggcgtg gtgaccgtga
     4681 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     4741 acttcccctc cgacggcccc gtaatgcaga agaaaaccat gggctgggag gcctcctccg
     4801 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     4861 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     4921 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     4981 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     5041 agctgtacaa gtaagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     5101 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5161 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5221 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag
     5281 gcatgctggg gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     5341 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     5401 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     5461 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     5521 ttgtggaaag gacgaaacac cgagggccga cttcccggcc caaaatcgag acagtagccg
     5581 taaaacgttg agtttcagcg tgggcgacac actcgaaaag gttaagatat gcacatagta
     5641 atccgtgcat gagccgcgaa agcggcttga aggtctatca ctgataggga gtattttatt
     5701 tttttgcggc cgcgtcgacg gcgcgcccta gacgaagcag ctccagccta cacaatcgct
     5761 caagacgtgt aatgctttta ttatatatta gtcacgatat ctataacaag aaaatatata
     5821 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     5881 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     5941 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     6001 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     6061 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaagg caacgcggcc
     6121 tttttacggt tcctggcctt ttgctggcct tttgctctag acgttacata acttacggta
     6181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6661 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg agcgagctga ttaaggagaa
     6721 catgcacatg aagctgtata tggagggcac cgtggacaac catcacttca agtgcacatc
     6781 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     6841 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     6901 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     6961 cacatgggag agagtcacca catacgagga cgggggcgtg ctgaccgcta cccaggacac
     7021 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     7081 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacactgta
     7141 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     7201 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     7261 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     7321 gacatacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     7381 ggggcacaag ctgaattaac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7441 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7501 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     7561 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagga tccgcacttt
     7621 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7681 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7741 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7801 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7861 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7921 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7981 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8041 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8101 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8161 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8221 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8281 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8341 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8401 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8461 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8521 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8581 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8881 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9421 ctttcctgcg t
//
