LOCUS       sequence-389005-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-based vector for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Baldanta S, Guevara G, Navarro-Llorens JM
  TITLE     SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in 
            cyanobacteria.
  JOURNAL   Microb Cell Fact. 2022 May 28;21(1):103. doi: 
            10.1186/s12934-022-01830-4.
  PUBMED    35643551
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-05-28"; volume: "21(1):103. doi"; pages: " 
            10.1186/s12934-022-01830-4"
COMMENT     Sequence Label: pSEVA351-Cpf1
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(647..1618)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      1725..2119
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(2157..2243)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(2354..2359,2360..2377,2378..2383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        6307..6341
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(6409..6705)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     6555..6577
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6569..6586
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6570..6586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6596..6615
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6596..6614
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6662..6682)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6662..6680)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6701..6717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6701..6717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6725..6741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6749..6755,6756..6773,6774..6779))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6794..6815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      7155..7249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        7297..7399
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7400..8059
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7466..7485)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     oriT            8208..8316
                     /note="incP origin of transfer"
     CDS             complement(8327..9178)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(join(9165..9868,1..136))
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
ORIGIN
        1 gcatggattt accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt
       61 tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag
      121 gcttatgggt agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg
      181 gtcactaccc ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta
      241 ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc
      301 atatcgcgct tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt
      361 ttggtctttc atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga
      421 acaaggacaa ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc
      481 cagcctcggc cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac
      541 ttttgatcag gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg
      601 tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat
      661 ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc
      721 gcgcaagctg gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg
      781 cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga
      841 tttcctcggc actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc
      901 tgaggtcatc accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg
      961 ccgtgcgccg gtggcggcta agctgccgct cgggcagttc gaggctggcc agcctgcggg
     1021 ccttctcctg ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg
     1081 ggccagcggt ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg
     1141 cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc
     1201 tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc
     1261 gaagttcacc gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg
     1321 tttccactac cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc
     1381 tgaggtcgtc caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt
     1441 cattgccctg ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg
     1501 accattcccg gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct
     1561 cacagcccat ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg
     1621 ggccaccaag cgattcccac acattatacg agccggaagc ataaagtgta gctagatccg
     1681 aaggatgagc cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc
     1741 ccacccttcg ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct
     1801 gcggggtttg gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt
     1861 ggggcggtgt gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata
     1921 ttgggcaagg gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct
     1981 tagataatca tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc
     2041 aggtttgggg gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga
     2101 cagttattgc aggggttcgt gacagttagg gcgcgcccag ctgtctaggg cggcggattt
     2161 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     2221 gactgagcct ttcgttttat ttgatgcctt taattaaagc ggataacaat ttcacacagg
     2281 aggccgccta ggccgcggcc gcgcgaattc gagctcggta cccggggatc ctcgatgtaa
     2341 cccactcgtg cactttacac tttatgcttc cggctcgtat gttaggaggt ctttatcatg
     2401 tcaatttatc aagaatttgt taataaatat agtttaagta aaactctaag atttgagtta
     2461 atcccacagg gtaaaacact tgaaaacata aaagcaagag gtttgatttt agatgatgag
     2521 aaaagagcta aagactacaa aaaggctaaa caaataattg ataaatatca tcagtttttt
     2581 atagaggaga tattaagttc ggtttgtatt agcgaagatt tattacaaaa ctattctgat
     2641 gtttatttta aacttaaaaa gagtgatgat gataatctac aaaaagattt taaaagtgca
     2701 aaagatacga taaagaaaca aatatctgaa tatataaagg actcagagaa atttaagaat
     2761 ttgtttaatc aaaaccttat cgatgctaaa aaagggcaag agtcagattt aattctatgg
     2821 ctaaagcaat ctaaggataa tggtatagaa ctatttaaag ccaatagtga tatcacagat
     2881 atagatgagg cgttagaaat aatcaaatct tttaaaggtt ggacaactta ttttaagggt
     2941 tttcatgaaa atagaaaaaa tgtttatagt agcaatgata ttcctacatc tattatttat
     3001 aggatagtag atgataattt gcctaaattt ctagaaaata aagctaagta tgagagttta
     3061 aaagacaaag ctccagaagc tataaactat gaacaaatta aaaaagattt ggcagaagag
     3121 ctaacctttg atattgacta caaaacatct gaagttaatc aaagagtttt ttcacttgat
     3181 gaagtttttg agatagcaaa ctttaataat tatctaaatc aaagtggtat tactaaattt
     3241 aatactatta ttggtggtaa atttgtaaat ggtgaaaata caaagagaaa aggtataaat
     3301 gaatatataa atctatactc acagcaaata aatgataaaa cactcaaaaa atataaaatg
     3361 agtgttttat ttaagcaaat tttaagtgat acagaatcta aatcttttgt aattgataag
     3421 ttagaagatg atagtgatgt agttacaacg atgcaaagtt tttatgagca aatagcagct
     3481 tttaaaacag tagaagaaaa atctattaaa gaaacactat ctttattatt tgatgattta
     3541 aaagctcaaa aacttgattt gagtaaaatt tattttaaaa atgataaatc tcttactgat
     3601 ctatcacaac aagtttttga tgattatagt gttattggta cagcggtact agaatatata
     3661 actcaacaaa tagcacctaa aaatcttgat aaccctagta agaaagagca agaattaata
     3721 gccaaaaaaa ctgaaaaagc aaaatactta tctctagaaa ctataaagct tgccttagaa
     3781 gaatttaata agcatagaga tatagataaa cagtgtaggt ttgaagaaat acttgcaaac
     3841 tttgcggcta ttccgatgat atttgatgaa atagctcaaa acaaagacaa tttggcacag
     3901 atatctatca aatatcaaaa tcaaggtaaa aaagacctac ttcaagctag tgcggaagat
     3961 gatgttaaag ctatcaagga tcttttagat caaactaata atctcttaca taaactaaaa
     4021 atatttcata ttagtcagtc agaagataag gcaaatattt tagacaagga tgagcatttt
     4081 tatctagtat ttgaggagtg ctactttgag ctagcgaata tagtgcctct ttataacaaa
     4141 attagaaact atataactca aaagccatat agtgatgaga aatttaagct caattttgag
     4201 aactcgactt tggctaatgg ttgggataaa aataaagagc ctgacaatac ggcaatttta
     4261 tttatcaaag atgataaata ttatctgggt gtgatgaata agaaaaataa caaaatattt
     4321 gatgataaag ctatcaaaga aaataaaggc gagggttata aaaaaattgt ttataaactt
     4381 ttacctggcg caaataaaat gttacctaag gttttctttt ctgctaaatc tataaaattt
     4441 tataatccta gtgaagatat acttagaata agaaatcatt ccacacatac aaaaaatggt
     4501 agtcctcaaa aaggatatga aaaatttgag tttaatattg aagattgccg aaaatttata
     4561 gatttttata aacagtctat aagtaagcat ccggagtgga aagattttgg atttagattt
     4621 tctgatactc aaagatataa ttctatagat gaattttata gagaagttga aaatcaaggc
     4681 tacaaactaa cttttgaaaa tatatcagag agctatattg atagcgtagt taatcagggt
     4741 aaattgtacc tattccaaat ctataataaa gatttttcag cttatagcaa agggcgacca
     4801 aatctacata ctttatattg gaaagcgctg tttgatgaga gaaatcttca agatgtggtt
     4861 tataagctaa atggtgaggc agagcttttt tatcgtaaac aatcaatacc taaaaaaatc
     4921 actcacccag ctaaagaggc aatagctaat aaaaacaaag ataatcctaa aaaagagagt
     4981 gtttttgaat atgatttaat caaagataaa cgctttactg aagataagtt tttctttcac
     5041 tgtcctatta caatcaattt taaatctagt ggagctaata agtttaatga tgaaatcaat
     5101 ttattgctaa aagaaaaagc aaatgatgtt catatattaa gtatagatag aggtgaaaga
     5161 catttagctt actatacttt ggtagatggt aaaggcaata tcatcaaaca agatactttc
     5221 aacatcattg gtaatgatag aatgaaaaca aactaccatg ataagcttgc tgcaatagag
     5281 aaagataggg attcagctag gaaagactgg aaaaagataa ataacatcaa agagatgaaa
     5341 gagggctatc tatctcaggt agttcatgaa atagctaagc tagttataga gtataatgct
     5401 attgtggttt ttgaggattt aaattttgga tttaaaagag ggcgtttcaa ggtagagaag
     5461 caggtctatc aaaagttaga aaaaatgcta attgagaaac taaactatct agttttcaaa
     5521 gataatgagt ttgataaaac tgggggagtg cttagagctt atcagctaac agcacctttt
     5581 gagactttta aaaagatggg taaacaaaca ggtattatct actatgtacc agctggtttt
     5641 acttcaaaaa tttgtcctgt aactggtttt gtaaatcagt tatatcctaa gtatgaaagt
     5701 gtcagcaaat ctcaagagtt ctttagtaag tttgacaaga tttgttataa ccttgataag
     5761 ggctattttg agtttagttt tgattataaa aactttggtg acaaggctgc caaaggcaag
     5821 tggactatag ctagctttgg gagtagattg attaacttta gaaattcaga taaaaatcat
     5881 aattgggata ctcgagaagt ttatccaact aaagagttgg agaaattgct aaaagattat
     5941 tctatcgaat atgggcatgg cgaatgtatc aaagcagcta tttgcggtga gagcgacaaa
     6001 aagttttttg ctaagctaac tagtgtccta aatactatct tacaaatgcg taactcaaaa
     6061 acaggtactg agttagatta tctaatttca ccagtagcag atgtaaatgg caatttcttt
     6121 gattcgcgac aggcgccaaa aaatatgcct caagatgctg atgccaatgg tgcttatcat
     6181 attgggctaa aaggtctgat gctactaggt aggatcaaaa ataatcaaga gggcaaaaaa
     6241 ctcaatttgg ttatcaaaaa tgaagagtat tttgagttcg tgcagaatag gaataactaa
     6301 gaattcttga cagctagctc agtcctaggt ataatgctag cgctgattta ggcaaaaacg
     6361 ggtctaagaa ctttaaataa tttctactgt tgtagatgag agcaggtgtc agccgctaca
     6421 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6481 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6541 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     6601 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc agcttttgtt
     6661 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6721 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6781 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6841 tccacccacc tgcaaaagtc taagaacttt aaataatttc tactgttgta gatgctacta
     6901 ttcctgtgcc ttcagataat tcagtctaag aactttaaat aatttctact gttgtagatg
     6961 tctagagcct tttgtattag tagccggtct aagaacttta aataatttct actgttgtag
     7021 attagcgatt tatgaaggtc atttttttgt ctagctttaa tgcggtagtt ggtaccgtcg
     7081 actctagaag ggacctgcag gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct
     7141 ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     7201 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
     7261 caataattac gatttaaatt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
     7321 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     7381 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     7441 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     7501 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     7561 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
     7621 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     7681 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     7741 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     7801 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     7861 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     7921 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     7981 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     8041 gtggcagggc ggggcgtaat ttgacttttg tccttttccg ctgcataacc ctgcttcggg
     8101 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     8161 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     8221 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     8281 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggcctcag cctgccgcct
     8341 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
     8401 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
     8461 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
     8521 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
     8581 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
     8641 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
     8701 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
     8761 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     8821 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
     8881 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     8941 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     9001 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     9061 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     9121 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     9181 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     9241 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     9301 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     9361 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     9421 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     9481 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     9541 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     9601 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     9661 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     9721 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     9781 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     9841 gcaatctgtg cggccagttg cagggcca
//
