LOCUS       sequence-389006-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacteria reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Schulte M, Olschewski K, Hensel M
  TITLE     The protected physiological state of intracellular Salmonella 
            enterica persisters reduces host cell-imposed stress.
  JOURNAL   Commun Biol. 2021 May 4;4(1):520. doi: 10.1038/s42003-021-02049-6.
  PUBMED    33947954
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-05-4"; volume: "4(1):520. doi"; pages: " 
            10.1038/s42003-021-02049-6"
COMMENT     Sequence Label: p5419
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             341..352
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1142..1169
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1199..1218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1200..1218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1228..1244)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1237..1259)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1386..1841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1473..1492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1683..1704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1867..1971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1972..2040,2041..2832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2190..2209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3853..4803)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4851..5073
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(6215..6235)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(6310..7023)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        7045..7100
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    7046..7064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7076..7094
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             7116..7742
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVISSALELLNEVGIEGLTTRKLAQRLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7192..7211)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(7978..8856)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcaggag gaattcacca tggtcgatat cggtaccggg aaagaggaga aaagtatggt
      361 gagcaaaggc gaagaactga ttaaagaaaa catgcatatg aaactgtata tggaaggcac
      421 cgtgaacaac catcatttta aatgcaccag cgaaggcgaa ggcaaaccgt atgaaggcac
      481 ccagaccatg cgcattaaag tggtggaagg cggtccgctg ccgtttgcgt ttgatattct
      541 ggcgaccagc tttatgtatg gcagccgcac ctttattaac catacccagg gcattccgga
      601 tttttttaaa cagagctttc cggaaggctt tacctgggaa cgcgtgacca cctatgaaga
      661 tggcggcgtg ctgaccgcga cccaggatac cagcctgcag gatggctgcc tgatttataa
      721 cgtgaaaatt cgtggcgtga actttccgag caatggcccg gtgatgcaga aaaaaaccct
      781 gggctgggaa gcgaacaccg aaatgctgta tccggcggat ggcggcctgg aaggccgtac
      841 cgatatggcg ctgaaactgg tgggcggtgg ccatctgatt tgcaacttta aaaccaccta
      901 tcgcagcaaa aaaccggcga aaaacctgaa aatgccgggc gtgtattatg tggatcatcg
      961 tctggaacgc attaaagaag cggataaaga aacctatgtg gaacagcatg aagtggcggt
     1021 ggcgcgttat tgcgatctgc cgagcaaact gggccataaa ctgaacggca tggatgaact
     1081 gtataaataa taaccatgga tcgatagctg gtgttaacga attccaggca tcaaattaag
     1141 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactgagct ccaattcgcc
     1201 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1261 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1321 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1381 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1441 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1501 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1561 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1621 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1681 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1741 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1801 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     1861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgctgg
     2461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2941 ccttaacgtg agttttcgtt ccactgagcg tcagatctct gttcgggggt tcactgacgc
     3001 tgcgtgacta cgatggtcag gttgcggagg ctatggccct ggtacgagcg ctgaacaaaa
     3061 tgacgaaagc aggtatgcct gaaagcgtgc gtattgcctg aaaacacaac ccgctacggg
     3121 ggagacttac ccgaaatctg atttattcaa caaagccact ttcagtatgg atttgggtgt
     3181 tacggggggg ggttggtgct taaaatcgcg ctgaggattt ctgatggtgt taagcgggcg
     3241 gttttgagat gtaaactcgc ccgtttaaca taatggatct tgcgcgcacc gcccgaacac
     3301 cactcgccac aaaaaaccgc cggaacgtcc aaaagtacgg gttttgctgc ccgcaaacgg
     3361 gctgttctgg tgttgctagt ttgttatcag aatcgcagat ccggcttcag ccggtttgcc
     3421 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
     3481 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
     3541 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
     3601 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
     3661 tcgatctgtt catggtgaac agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta
     3721 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatgtaa
     3781 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     3841 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     3901 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa
     3961 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     4021 tcttagtccg ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     4081 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     4141 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     4201 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     4261 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     4321 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     4381 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     4441 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     4501 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     4561 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     4621 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     4681 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     4741 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     4801 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     4861 atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     4921 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     4981 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     5041 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     5101 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     5161 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     5221 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     5281 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     5341 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgcgga
     5401 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     5461 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     5521 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa
     5581 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     5641 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     5701 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     5761 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     5821 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     5881 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     5941 ccggcaccct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     6001 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggc
     6061 attcgccatt caggctgcct cctaccaaga cgacttcaaa tccgacccgt agagatcgta
     6121 ccgggccccc cctcgagaaa aaaaagcccg ctcattaggc gggctagctc attatttgta
     6181 gagctcatcc atgccatgtg taatcccagc agcagttaca aactcaagaa ggaccatgtg
     6241 gtcacgcttt tcgttgggat ctttcgaaag ggcagattgt gtcgaccagc tatcgatcca
     6301 tggttattat ttatacagtt catccatgcc atgcgtaata cccgccgcgg tcacaaattc
     6361 cagcagcacc atatggtcgc gtttttcgtt cggatctttg ctcaggacgc tctgggtgct
     6421 cagataatgg ttatccggca gcagcaccgg gccatcgcca atcggggtgt tctgctgata
     6481 atgatccgcc agctgcacgc tgccatcttc cacgttgtgg cggattttaa agttcgcttt
     6541 gatgccgttt ttctgtttat ccgcggtaat atacacgtta tggctgttaa agttgtattc
     6601 cagtttatgg cccaggatgt tgccatcttc tttaaaatcg atgcctttca gttcaatgcg
     6661 gttcaccagg gtatcgcctt caaatttcac ttccgcacgg gttttatagg tgccatcatc
     6721 tttaaagcta atggtgcgtt cctgcacata gccttccggc atcgcgcttt taaaaaaatc
     6781 atgctgtttc atatgatccg gataacgcgc aaagcactgc acgccatagg tcagggtggt
     6841 caccagggtc ggccacggca ccggcagttt gcccgtggtg caaatgaatt tcagggtcag
     6901 tttgccgttg gtcgcatcgc cttcgccttc gccacgcacg ctaaatttat ggccgttcac
     6961 atcgccatcc agttccacca gaatcggcac cacaccggta aacagttctt cgcctttgcg
     7021 catgatatcc tcctcttgcc atctttctct atcactgata gggagtggta aaataactct
     7081 atcaatgata gagtgtcaac aaaaattagg aattaatgat gtctagatta gataaaagta
     7141 aagtgattag cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     7201 gtaaactcgc ccagaggcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     7261 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     7321 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     7381 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     7441 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     7501 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     7561 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     7621 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     7681 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctt
     7741 aaaagcagca taaccttttt ccgtgatggt aacttcacgg taaccaagat gtcgagttaa
     7801 ccacccatcg aagccaccgc ggtggagaga aaataataaa aaagccggat taataatctg
     7861 gctttttata ttctctgcgg ccatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg
     7921 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
     7981 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
     8041 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
     8101 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
     8161 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
     8221 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
     8281 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
     8341 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
     8401 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
     8461 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat
     8521 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
     8581 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
     8641 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
     8701 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
     8761 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
     8821 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
     8881 ag
//
