LOCUS       sequence-389008-       10082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-based vector for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Baldanta S, Guevara G, Navarro-Llorens JM
  TITLE     SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in 
            cyanobacteria.
  JOURNAL   Microb Cell Fact. 2022 May 28;21(1):103. doi: 
            10.1186/s12934-022-01830-4.
  PUBMED    35643551
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-05-28"; volume: "21(1):103. doi"; pages: " 
            10.1186/s12934-022-01830-4"
COMMENT     Sequence Label: pSEVA451-Cpf1
FEATURES             Location/Qualifiers
     source          1..10082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(105..110,111..128,129..134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        4058..4092
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(4160..4456)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4306..4328
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4320..4337
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4321..4337
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4347..4366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4347..4365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4413..4433)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4413..4431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4452..4468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4452..4468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4476..4492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4500..4506,4507..4524,4525..4530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4545..4566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      4906..5000
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5236..6027
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            6173..6281
                     /note="incP origin of transfer"
     CDS             complement(6292..7143)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(7130..7969)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8480..9451)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      9558..9952
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(9990..10076)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctcgatgta acccactcgt gcactttaca ctttatgctt
      121 ccggctcgta tgttaggagg tctttatcat gtcaatttat caagaatttg ttaataaata
      181 tagtttaagt aaaactctaa gatttgagtt aatcccacag ggtaaaacac ttgaaaacat
      241 aaaagcaaga ggtttgattt tagatgatga gaaaagagct aaagactaca aaaaggctaa
      301 acaaataatt gataaatatc atcagttttt tatagaggag atattaagtt cggtttgtat
      361 tagcgaagat ttattacaaa actattctga tgtttatttt aaacttaaaa agagtgatga
      421 tgataatcta caaaaagatt ttaaaagtgc aaaagatacg ataaagaaac aaatatctga
      481 atatataaag gactcagaga aatttaagaa tttgtttaat caaaacctta tcgatgctaa
      541 aaaagggcaa gagtcagatt taattctatg gctaaagcaa tctaaggata atggtataga
      601 actatttaaa gccaatagtg atatcacaga tatagatgag gcgttagaaa taatcaaatc
      661 ttttaaaggt tggacaactt attttaaggg ttttcatgaa aatagaaaaa atgtttatag
      721 tagcaatgat attcctacat ctattattta taggatagta gatgataatt tgcctaaatt
      781 tctagaaaat aaagctaagt atgagagttt aaaagacaaa gctccagaag ctataaacta
      841 tgaacaaatt aaaaaagatt tggcagaaga gctaaccttt gatattgact acaaaacatc
      901 tgaagttaat caaagagttt tttcacttga tgaagttttt gagatagcaa actttaataa
      961 ttatctaaat caaagtggta ttactaaatt taatactatt attggtggta aatttgtaaa
     1021 tggtgaaaat acaaagagaa aaggtataaa tgaatatata aatctatact cacagcaaat
     1081 aaatgataaa acactcaaaa aatataaaat gagtgtttta tttaagcaaa ttttaagtga
     1141 tacagaatct aaatcttttg taattgataa gttagaagat gatagtgatg tagttacaac
     1201 gatgcaaagt ttttatgagc aaatagcagc ttttaaaaca gtagaagaaa aatctattaa
     1261 agaaacacta tctttattat ttgatgattt aaaagctcaa aaacttgatt tgagtaaaat
     1321 ttattttaaa aatgataaat ctcttactga tctatcacaa caagtttttg atgattatag
     1381 tgttattggt acagcggtac tagaatatat aactcaacaa atagcaccta aaaatcttga
     1441 taaccctagt aagaaagagc aagaattaat agccaaaaaa actgaaaaag caaaatactt
     1501 atctctagaa actataaagc ttgccttaga agaatttaat aagcatagag atatagataa
     1561 acagtgtagg tttgaagaaa tacttgcaaa ctttgcggct attccgatga tatttgatga
     1621 aatagctcaa aacaaagaca atttggcaca gatatctatc aaatatcaaa atcaaggtaa
     1681 aaaagaccta cttcaagcta gtgcggaaga tgatgttaaa gctatcaagg atcttttaga
     1741 tcaaactaat aatctcttac ataaactaaa aatatttcat attagtcagt cagaagataa
     1801 ggcaaatatt ttagacaagg atgagcattt ttatctagta tttgaggagt gctactttga
     1861 gctagcgaat atagtgcctc tttataacaa aattagaaac tatataactc aaaagccata
     1921 tagtgatgag aaatttaagc tcaattttga gaactcgact ttggctaatg gttgggataa
     1981 aaataaagag cctgacaata cggcaatttt atttatcaaa gatgataaat attatctggg
     2041 tgtgatgaat aagaaaaata acaaaatatt tgatgataaa gctatcaaag aaaataaagg
     2101 cgagggttat aaaaaaattg tttataaact tttacctggc gcaaataaaa tgttacctaa
     2161 ggttttcttt tctgctaaat ctataaaatt ttataatcct agtgaagata tacttagaat
     2221 aagaaatcat tccacacata caaaaaatgg tagtcctcaa aaaggatatg aaaaatttga
     2281 gtttaatatt gaagattgcc gaaaatttat agatttttat aaacagtcta taagtaagca
     2341 tccggagtgg aaagattttg gatttagatt ttctgatact caaagatata attctataga
     2401 tgaattttat agagaagttg aaaatcaagg ctacaaacta acttttgaaa atatatcaga
     2461 gagctatatt gatagcgtag ttaatcaggg taaattgtac ctattccaaa tctataataa
     2521 agatttttca gcttatagca aagggcgacc aaatctacat actttatatt ggaaagcgct
     2581 gtttgatgag agaaatcttc aagatgtggt ttataagcta aatggtgagg cagagctttt
     2641 ttatcgtaaa caatcaatac ctaaaaaaat cactcaccca gctaaagagg caatagctaa
     2701 taaaaacaaa gataatccta aaaaagagag tgtttttgaa tatgatttaa tcaaagataa
     2761 acgctttact gaagataagt ttttctttca ctgtcctatt acaatcaatt ttaaatctag
     2821 tggagctaat aagtttaatg atgaaatcaa tttattgcta aaagaaaaag caaatgatgt
     2881 tcatatatta agtatagata gaggtgaaag acatttagct tactatactt tggtagatgg
     2941 taaaggcaat atcatcaaac aagatacttt caacatcatt ggtaatgata gaatgaaaac
     3001 aaactaccat gataagcttg ctgcaataga gaaagatagg gattcagcta ggaaagactg
     3061 gaaaaagata aataacatca aagagatgaa agagggctat ctatctcagg tagttcatga
     3121 aatagctaag ctagttatag agtataatgc tattgtggtt tttgaggatt taaattttgg
     3181 atttaaaaga gggcgtttca aggtagagaa gcaggtctat caaaagttag aaaaaatgct
     3241 aattgagaaa ctaaactatc tagttttcaa agataatgag tttgataaaa ctgggggagt
     3301 gcttagagct tatcagctaa cagcaccttt tgagactttt aaaaagatgg gtaaacaaac
     3361 aggtattatc tactatgtac cagctggttt tacttcaaaa atttgtcctg taactggttt
     3421 tgtaaatcag ttatatccta agtatgaaag tgtcagcaaa tctcaagagt tctttagtaa
     3481 gtttgacaag atttgttata accttgataa gggctatttt gagtttagtt ttgattataa
     3541 aaactttggt gacaaggctg ccaaaggcaa gtggactata gctagctttg ggagtagatt
     3601 gattaacttt agaaattcag ataaaaatca taattgggat actcgagaag tttatccaac
     3661 taaagagttg gagaaattgc taaaagatta ttctatcgaa tatgggcatg gcgaatgtat
     3721 caaagcagct atttgcggtg agagcgacaa aaagtttttt gctaagctaa ctagtgtcct
     3781 aaatactatc ttacaaatgc gtaactcaaa aacaggtact gagttagatt atctaatttc
     3841 accagtagca gatgtaaatg gcaatttctt tgattcgcga caggcgccaa aaaatatgcc
     3901 tcaagatgct gatgccaatg gtgcttatca tattgggcta aaaggtctga tgctactagg
     3961 taggatcaaa aataatcaag agggcaaaaa actcaatttg gttatcaaaa atgaagagta
     4021 ttttgagttc gtgcagaata ggaataacta agaattcttg acagctagct cagtcctagg
     4081 tataatgcta gcgctgattt aggcaaaaac gggtctaaga actttaaata atttctactg
     4141 ttgtagatga gagcaggtgt cagccgctac agggcgcgtc ccattcgcca ttcaggctgc
     4201 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4261 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     4321 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
     4381 ggccccccct cgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     4441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4561 acattaattg cgttgcgctc actgcccgct ttccacccac ctgcaaaagt ctaagaactt
     4621 taaataattt ctactgttgt agatgctact attcctgtgc cttcagataa ttcagtctaa
     4681 gaactttaaa taatttctac tgttgtagat gtctagagcc ttttgtatta gtagccggtc
     4741 taagaacttt aaataatttc tactgttgta gattagcgat ttatgaaggt catttttttg
     4801 tctagcttta atgcggtagt tggtaccgtc gactctagaa gggacctgca ggcatgcaag
     4861 cttgcggccg cgtcgtgact gggaaaaccc tggcgactag tcttggactc ctgttgatag
     4921 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     4981 cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttacgt atttaaatga
     5041 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     5101 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     5161 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     5221 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     5281 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     5341 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     5401 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     5461 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     5521 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     5581 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     5641 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     5701 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     5761 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     5821 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     5881 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     5941 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6001 gagatcacca aggtagtcgg caaataagac aattgtcctt ttccgctgca taaccctgct
     6061 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     6121 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     6181 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     6241 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc ctcagcctgc
     6301 cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg tccagcccag
     6361 cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc
     6421 actggcctct gacggccaga catagccgca caaggtatct atggaagcct tgccggtttt
     6481 gccggggtcg atccagccac acagccgctg gtgcagcagg cgggcggttt cgctgtccag
     6541 cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg ccacccatga cggcctgcgc
     6601 gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga
     6661 cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata ccttccaaag
     6721 gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct ctgccccgat
     6781 ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg tgacggcctc
     6841 ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg gttccgggcc
     6901 aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc gcagatcatc
     6961 agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt
     7021 cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc
     7081 cagacagtgc gccgggtcgt gccggacgtg gctgaggctg tgcttgttct taggcttcac
     7141 cacggggcac ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc
     7201 cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc atagttggcc ttgctcacac
     7261 cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc
     7321 tggtcatgct cgacaggtag gactgccagc ggatgttatc gaccagtacc gagctgcccc
     7381 ggctggcctg ctgctggtcg cctgcgccca tcatggccgc gcccttgctg gcatggtgca
     7441 ggaacacgat agagcacccg gtatcggcgg cgatggcctc catgcgaccg atgacctggg
     7501 ccatggggcc gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca
     7561 tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt
     7621 tgggcaggct gccgatcagc ggctggatca gcaggccgtc agccacggct tgccgttcct
     7681 cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt
     7741 cggcgggcag gtagatcacc gggccggtgg gcagttcgcc cacctccagc agatccggcc
     7801 cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga tttaccggca ccaccgggcg
     7861 acaccagcgc cccgaccgta ccggccacca tgttgggcaa aacgtagtcc agcggtggcg
     7921 gcgctgctgc gaacgcctcc agaatattga taggcttatg ggtagccatt gattgcctcc
     7981 tttgcaggca gttggtggtt aggcgctggc ggggtcacta cccccgccct gcgccgctct
     8041 gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg
     8101 ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg
     8161 gctggccagc aggtcgccgg tctgcttgtc cttttggtct ttcatatcag tcaccgagaa
     8221 acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt
     8281 taaggctggc catatcagcg actgaaaagc ggccagcctc ggccttgttt gacgtataac
     8341 caaagccacc gggcaaccaa tagcccttgt cacttttgat caggtagacc gaccctgaag
     8401 cgcttttttc gtattccata aaaccccctt ctgtgcgtga gtactcatag tataacaggc
     8461 gtgagtacca acgcaagcac tacatgctga aatctggccc gcccctgtcc atgcctcgct
     8521 ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg ggcagaccca
     8581 tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca
     8641 gcgctgggct ggcctcggcc atggccttgc cgatttcctc ggcactgcgg ccccggctgg
     8701 ccagcttctg cgcggcgata aagtcgcact tgctgaggtc atcaccgaag cgcttgacca
     8761 gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc
     8821 gctcgggcag ttcgaggctg gccagcctgc gggccttctc ctgctgccgc tgggcctgct
     8881 cgatctgctg gccagcctgc tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg
     8941 attcacgcag cagcacccac ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt
     9001 tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt
     9061 actcgctggc cagcgtccgg gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca
     9121 ccttgaccca tgcctgatag ttcttcgggc tggtttccac taccagggca ggctcccggc
     9181 cctcggcttt catgtcatcc aggtcaaact cgctgaggtc gtccaccagc accagaccat
     9241 gccgctcctg ctcggcgggc ctgatataca cgtcattgcc ctgggcattc atccgcttga
     9301 gccatggcgt gttctggagc acttcggcgg ctgaccattc ccggttcatc atctggccgg
     9361 tggtggcgtc cctgacgccg atatcgaagc gctcacagcc catggccttg agctgtcggc
     9421 ctatggcctg caaagtcctg tcgttcttca tcgggccacc aagcgattcc cacacattat
     9481 acgagccgga agcataaagt gtagctagat ccgaaggatg agccgggctg aatgatcgac
     9541 cgagacaggc cctgcggggc tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg
     9601 ctaaagcggc taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg
     9661 ctttgcccgc ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga
     9721 atagaccagc tatccggcct ctggccgggc atattgggca agggcagcag cgccccacaa
     9781 gggcgctgat aaccgcgcct agtggattat tcttagataa tcatggatgg atttttccaa
     9841 caccccgcca gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac agttattgca
     9901 ggggttcgtg acagttattg caggggggcg tgacagttat tgcaggggtt cgtgacagtt
     9961 agggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
    10021 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
    10081 ct
//
