LOCUS       sequence-389032-       11461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0508
FEATURES             Location/Qualifiers
     source          1..11461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7318..7906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7371..7391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7789..7800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7978..8211
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8289..8410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8326..8345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8380..8399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8450..8585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8605..8624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8606..8624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8634..8651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8634..8650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8643..8665)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8792..9247
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8879..8898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9089..9110
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9273..9377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9378..9446,9447..10238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9596..9615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10409..10997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10898..10917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11151..11168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11285..11306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11321..11326,11327..11344,11345..11351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11359..11375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11364..11386
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11383..11399
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11383..11399
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11418..11438
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11420..11438
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatg gctgctgcgt tgctccatta
     4141 tacaggctac gcccaggagg aacagctgaa agaagaggag gaaataaaag aagaggagga
     4201 ggaggaggac agcggttcag tagctcaact gcagggtggc aatgacacag ggacggacga
     4261 ggagctagaa acgggcccag agcaaaaagg ctgcttcagc taccagaact ctccaggaag
     4321 tcatttgtcc aatcaggatg ccgagaacga gtctctgctg agtgacgcca gtgatcaggt
     4381 gtcggacatc aagagtgtct gcggcagaga tgcctcagac aagaaagcac acactcacgt
     4441 caggcttcca aacgaagcac acaattgcat ggataaaatg accgctgtct acgccaacat
     4501 cctgtcggat tcctactggt caggcctggg ccttggcttc aagctgtcca atagtgagag
     4561 gaggaactgt gacacccgaa acggcagcaa caagagtgat tttgattggc accaagacgc
     4621 tctgtccaaa agcctgcagc agaacttgcc ttctcggtcc gtctcgaaac ccagcctgtt
     4681 cagctcggtg cagttgtacc gacagagcag caagatgtgc gggactgtgt tcacaggggc
     4741 cagcagattc cgatgccgac agtgcagcgc ggcctatgac accctagtcg agctgactgt
     4801 gcacatgaat gaaacgggcc actatcaaga tgacaaccgc aaaaaggaca agctcagacc
     4861 cacgagctat tcaaagccca ggaaaagggc tttccaggat atggacaaag aggatgctca
     4921 aaaggttctg aaatgtatgt tttgtggcga ctcctttgat tccctccaag atttgagcgt
     4981 ccacatgatt aaaacaaaac attaccaaaa agtgcctttg aaggagccag tcccaaccat
     5041 ttcctcgaaa atggtcaccc cggctaagaa acgcgttttt gatgtcaatc ggccgtgttc
     5101 ccccgattca accacaggat cttttgcaga ttctttttct tctcagaaga acgccaactt
     5161 gcagttgtcc tccaacaacc gctatggcta ccaaaatgga gccagctaca cctggcagtt
     5221 tgaggcctgc aagtcccaga tcttaaagtg catggagtgt gggagctccc atgacacctt
     5281 gcagcagctc accacccaca tgatggtcac aggtcacttt ctcaaggtca ccagctctgc
     5341 ctccaagaaa gggaagcagc tggtattaga cccgttagca gtggagaaaa tgcagtcgtt
     5401 gtctgaggcc ccaaacagtg attctctggc tcccaagcca tccagtaact cagcatcaga
     5461 ttgtacagcc tctacaactg agttaaagaa agagagtaaa aaagaaaggc cagaggaaac
     5521 cagcaaggat gagaaagtcg tgaaaagcga ggactatgaa gatcctctac aaaaaccttt
     5581 agaccctaca atcaaatatc aatacctaag ggaggaagac ttggaagatg gctcaaaggg
     5641 tggaggggac attttgaaat ctttggaaaa tactgtcacc acagccatca acaaagccca
     5701 aaacggggcc cccagctgga gtgcctaccc cagcatccac gcagcctacc agctgtctga
     5761 gggcaccaag ccgcctttgc ctatgggatc ccaggtactg cagatccggc ctaatctcac
     5821 caacaagctg aggcccattg caccaaagtg gaaagtgatg ccactggttt ctatgcccac
     5881 acacctggcc ccttacactc aagtcaagaa agagtcagaa gacaaagatg aagcggtgaa
     5941 ggagtgtggg aaagaaagtc cccacgaaga ggcctcatct ttcagccaca gtgagggcga
     6001 ttctttccgc aaaagtgaaa cacctccaga agccaaaaag accgagctgg gtcccctgaa
     6061 ggaggaggag aagctgatga aagagggcag cgagaaggag aaaccccagc ccctggagcc
     6121 cacatctgct ctgagcaatg ggtgcgccct cgccaaccac gccccggccc tgccatgcat
     6181 caacccactc agcgccctgc agtccgtcct gaacaatcac ttgggcaaag ccacggagcc
     6241 cttgcgctca ccttcctgct ccagcccaag ttcaagcaca atttccatgt tccacaagtc
     6301 gaatctcaat gtcatggaca agccggtctt gagtcctgcc tccacaaggt cagccagcgt
     6361 gtccaggcgc tacctgtttg agaacagcga tcagcccatt gacctgacca agtccaaaag
     6421 caagaaagcc gagtcctcgc aagcacaatc ttgtatgtcc ccacctcaga agcacgctct
     6481 gtctgacatc gccgacatgg tcaaagtcct ccccaaagcc accaccccaa agccagcctc
     6541 ctcctccagg gtccccccca tgaagctgga aatggatgtc aggcgctttg aggatgtctc
     6601 cagtgaagtc tcaactttgc ataaaagaaa aggccggcag tccaactgga atcctcagca
     6661 tcttctgatt ctacaagccc agtttgcctc gagcctcttc cagacatcag agggcaaata
     6721 cctgctgtct gatctgggcc cacaagagcg tatgcaaatc tctaagttta cgggactctc
     6781 aatgaccact atcagtcact ggctggccaa cgtcaagtac cagcttagga aaacgggcgg
     6841 gacaaaattt ctgaaaaaca tggacaaagg ccaccccatc ttttattgca gtgactgtgc
     6901 ctcccagttc agaacccctt ctacctacat cagtcactta gaatctcacc tgggtttcca
     6961 aatgaaggac atgacccgct tgtcagtgga ccagcaaagc aaggtggagc aagagatctc
     7021 ccgggtatcg tcggctcaga ggtctccaga aacaatagct gccgaagagg acacagactc
     7081 taaattcaag tgtaagttgt gctgtcggac atttgtgagc aaacatgcgg taaaactcca
     7141 cctaagcaaa acgcacagca agtcacccga acaccattca cagtttgtaa cagacgtgga
     7201 tgaagaatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     7261 atcttgtgga aaggacgaca atttccaaca gtcgtcctgc gaacgcgtta agtcgacaat
     7321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7681 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     7921 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7981 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8041 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8101 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8161 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     8221 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8281 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8401 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8581 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8641 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8701 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8761 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8821 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8881 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8941 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9001 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9061 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9121 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9181 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9241 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9601 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11161 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11221 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11281 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    11341 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    11401 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    11461 c
//
