LOCUS       sequence-389045-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_S36X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_S36X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        508..711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          847..979
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     962..981
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1023..1042
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1023..1041
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6145..6657
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6733..6751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6788..6909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6799..6818)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6853..6872
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7154..7511
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7154..7345
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7177..7197)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7362..7497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7542..8579
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8558..8566
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8603..8651
                     /note="synthetic polyadenylation signal"
     promoter        8807..8911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8912..8980,8981..9772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9130..9149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9930..10518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10010..10029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10634..10917
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg
       61 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
      121 ccatgttggc attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      721 aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat tgctaacgca
      781 gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc gtgaggcact
      841 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
      901 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
      961 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     1021 attaatacga ctcactatag ggctagcaaa gcgatcgcca tgaaactgcg tggagtcagc
     1081 ctggctgccg gcttgttctt actggccctg agtctttggg ggcagcctgc agaggctgcg
     1141 gcttgctatg ggtgttctcc aggatgaaag tgtgactgca gtggcataaa aggggaaaag
     1201 ggagagagag ggtttccagg tttggaagga cacccaggat tgcctggatt tccaggtcca
     1261 gaagggcctc cggggcctcg gggacaaaag ggtgatgatg gaattccagg gccaccagga
     1321 ccaaaaggaa tcagaggtcc tcctggactt cctggatttc cagggacacc aggtcttcct
     1381 ggaatgccag gccacgatgg ggccccagga cctcaaggta ttcccggatg caatggaacc
     1441 aagggagaac gtggatttcc aggcagtccc ggttttcctg gtttacaggg tcctccagga
     1501 ccccctggga tcccaggtat gaagggtgaa ccaggtagta taattatgtc atcactgcca
     1561 ggaccaaagg gtaatccagg atatccaggt cctcctggaa tacaaggcct acctggtccc
     1621 actggtatac cagggccaat tggtccccca ggaccaccag gtttgatggg ccctcctggt
     1681 ccaccaggac ttccaggacc taaggggaat atgggcttaa atttccaggg acccaaaggt
     1741 gaaaaaggtg agcaaggtct tcagggccca cctgggccac ctgggcagat cagtgaacag
     1801 aaaagaccaa ttgatgtaga gtttcagaaa ggagatcagg gacttcctgg tgaccgaggg
     1861 cctcctggac ctccagggat acgtggtcct ccaggtcccc caggtggtga gaaaggtgag
     1921 aagggtgagc aaggagagcc aggcaaaaga ggtaaaccag gcaaagatgg agaaaatggc
     1981 caaccaggaa ttcctggttt gcctggtgat cctggttacc ctggtgaacc cggaagggat
     2041 ggtgaaaagg gccaaaaagg tgacactggc ccacctggac ctcctggact tgtaattcct
     2101 agacctggga ctggtataac tataggagaa aaaggaaaca ttgggttgcc tgggttgcct
     2161 ggagaaaaag gagagcgagg atttcctgga atacagggtc cacctggcct tcctggacct
     2221 ccaggggctg cagttatggg tcctcctggc cctcctggat ttcctggaga aaggggtcag
     2281 aaaggtgatg aaggaccacc tggaatttcc attcctggac ctcctggact tgacggacag
     2341 cctggggctc ctgggcttcc agggcctcct ggccctgctg gccctcacat tcctcctagt
     2401 gatgagatat gtgaaccagg ccctccaggc cccccaggat ctccaggtga taaaggactc
     2461 caaggagaac aaggagtgaa aggtgacaaa ggtgacactt gcttcaactg cattggaact
     2521 ggtatttcag ggcctccagg tcaacctggt ttgccaggtc tcccaggtcc tccaggatct
     2581 cttggtttcc ctggacagaa aggggaaaaa ggacaagctg gtgcaactgg tcccaaagga
     2641 ttaccaggca ttccaggagc tccaggtgct ccaggctttc ctggatctaa aggtgaacct
     2701 ggtgatatcc tcacttttcc aggaatgaag ggtgacaaag gagagttggg ttcccctgga
     2761 gctccagggc ttcctggttt acctggcact cctggacagg atggattgcc agggcttcct
     2821 ggcccgaaag gagagcctgg tggaattact tttaagggtg aaagaggtcc ccctgggaac
     2881 ccaggtttac caggcctccc agggaatata gggcctatgg gtccccctgg tttcggccct
     2941 ccaggcccag taggtgaaaa aggcatacaa ggtgtggcag gaaatccagg ccagccagga
     3001 ataccaggtc ctaaagggga tccaggtcag actataaccc agccggggaa gcctggcttg
     3061 cctggtaacc caggcagaga tggtgatgta ggtcttccag gtgaccctgg acttccaggg
     3121 caaccaggct tgccagggat acctggtagc aaaggagaac caggtatccc tggaattggg
     3181 cttcctggac cacctggtcc caaaggcttt cctggaattc caggacctcc aggagcacct
     3241 gggacacctg gaagaattgg tctagaaggc cctcctgggc cacccggctt tccaggacca
     3301 aagggtgaac caggatttgc attacctggg ccacctgggc caccaggact tccaggtttc
     3361 aaaggagcac ttggtccaaa aggtgatcgt ggtttcccag gacctccggg tcctccagga
     3421 cgcactggct tagatgggct ccctggacca aaaggtgatg ttggaccaaa tggacaacct
     3481 ggaccaatgg gacctcctgg gctgccagga ataggtgttc agggaccacc aggaccacca
     3541 gggattcctg ggccaatagg tcaacctggt ttacatggaa taccaggaga gaagggggat
     3601 ccaggacctc ctggacttga tgttccagga cccccaggtg aaagaggcag tccagggatc
     3661 cccggagcac ctggtcctat aggacctcca ggatcaccag ggcttccagg aaaagcaggt
     3721 gcctctggat ttccaggtac caaaggtgaa atgggtatga tgggacctcc aggcccacca
     3781 ggacctttgg gaattcctgg caggagtggt gtacctggtc ttaaaggtga tgatggcttg
     3841 cagggtcagc caggacttcc tggccctaca ggagaaaaag gtagtaaagg agagcctggc
     3901 cttccaggcc ctcctggacc aatggatcca aatcttctgg gctcaaaagg agagaagggg
     3961 gaacctggct taccaggtat acctggagtt tcagggccaa aaggttatca gggtttgcct
     4021 ggagacccag ggcaacctgg actgagtgga caacctggat taccaggacc accaggtccc
     4081 aaaggtaacc ctggtctccc tggacagcca ggtcttatag gacctcctgg acttaaagga
     4141 accatcggtg atatgggttt tccagggcct cagggtgtgg aagggcctcc tggaccttct
     4201 ggagttcctg gacaacctgg ctccccagga ttacctggac agaaaggcga caaaggtgat
     4261 cctggtattt caagcattgg tcttccaggt cttcctggtc caaagggtga gcctggtctg
     4321 cctggatacc cagggaaccc tggtatcaaa ggttctgtgg gagatcctgg tttgcccgga
     4381 ttaccaggaa cccctggagc aaaaggacaa ccaggccttc ctggattccc aggaacccca
     4441 ggccctcctg gaccaaaagg tattagtggc cctcctggga accccggcct tccaggagaa
     4501 cctggtcctg taggtggtgg aggtcatcct gggcaaccag ggcctccagg cgaaaaaggc
     4561 aaacccggtc aagatggtat tcctggacca gctggacaga agggtgaacc aggtcaacca
     4621 ggctttggaa acccaggacc gcctggactt ccaggacttt ctggccaaaa gggtgatgga
     4681 ggattacctg ggattccagg aaatcctggc cttccaggtc caaagggcga accaggcttt
     4741 cacggtttcc ctggtgtgca gggtccccca ggccctcctg gttctccggg tccagctctg
     4801 gaaggaccta aaggcaaccc tgggccccaa ggtcctcctg ggagaccagg tcctacaggt
     4861 tttcaaggtc taccaggtcc agaaggtcct ccaggtctcc ctggaaatgg aggtattaaa
     4921 ggagagaagg gaaatccagg ccaacctggg ctacctggct tgcctggttt gaaaggagat
     4981 caaggaccac caggactcca gggtaatcct ggccggccgg gtctcaatgg aatgaaagga
     5041 gatcctggtc tccctggtgt tccaggattc ccaggcatga aaggacccag tggagtacct
     5101 ggatcagctg gccctgaggg ggaaccggga cttattggtc ctccaggtcc tcctggatta
     5161 cctggtcctt caggacagag tatcataatt aaaggagatg ctggtcctcc aggaatccct
     5221 ggccagcctg ggctaaaggg tctaccagga ccccaaggac ctcaaggctt accaggtcca
     5281 actggccctc caggagatcc tggacgcaat ggactccctg gctttgatgg tgcaggaggg
     5341 cgcaaaggag acccaggtct gccaggacag ccaggtaccc gtggtttgga tggtccccct
     5401 ggtccagatg gattgcaagg tcccccaggt ccccctggaa cctcctctgt tgcacatgga
     5461 tttcttatta cacgccacag ccagacaacg gatgcaccac aatgcccaca gggaacactt
     5521 caggtctatg aaggcttttc tctcctgtat gtacaaggaa ataaaagagc ccacggtcaa
     5581 gacttgggga cggctggcag ctgccttcgt cgctttagta ccatgccttt catgttctgc
     5641 aacatcaata atgtttgcaa ctttgcttca agaaatgact attcttactg gctctctacc
     5701 ccagagccca tgccaatgag catgcaaccc ctaaagggcc agagcatcca gccattcatt
     5761 agtcgatgtg cagtatgtga agctccagct gtggtgatcg cagttcacag tcagacgatc
     5821 cagattcccc attgtcctca gggatgggat tctctgtgga ttggttattc cttcatgatg
     5881 catacaagtg caggggcaga aggctcaggt caagccctag cctcccctgg ttcctgcttg
     5941 gaagagtttc gttcagctcc cttcatcgaa tgtcatggga ggggtacctg taactactat
     6001 gccaactcct acagcttttg gctggcaact gtagatgtgt cagacatgtt cagtaaacct
     6061 cagtcagaaa cgctgaaagc aggagacttg aggacacgaa ttagccgatg tcaagtgtgc
     6121 atgaagagga cattctcgag cggcgtcttc acactcgaag atttcgttgg ggactggcga
     6181 cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt
     6241 cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg
     6301 ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc
     6361 cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc
     6421 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga
     6481 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg
     6541 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc
     6601 cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgtaaggc
     6661 cgcgactcta gggcatgcaa gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
     6721 tggctgctgc caccgctgag caataactag cataacccct tggggcggcc gcttcgagca
     6781 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     6841 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6901 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     6961 gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcgaatt ttaacaaaat
     7021 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7081 caccgcatac gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     7141 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     7201 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7261 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7321 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7381 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7441 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7501 gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgaagaag cccgaactca
     7561 ccgctaccag cgttgaaaaa tttctcatcg agaagttcga cagtgtgagc gacctgatgc
     7621 agttgtcgga gggcgaagag agccgagcct tcagcttcga tgtcggcgga cgcggctatg
     7681 tactgcgggt gaatagctgc gctgatggct tctacaaaga ccgctacgtg taccgccact
     7741 tcgccagcgc tgcactaccc atccccgaag tgttggacat cggcgagttc agcgagagcc
     7801 tgacatactg catcagtaga cgcgcccaag gcgttactct ccaagacctc cccgaaacag
     7861 agctgcctgc tgtgttacag cctgtcgccg aagctatgga tgctattgcc gccgccgacc
     7921 tcagtcaaac cagcggcttc ggcccattcg ggccccaagg catcggccag tacacaacct
     7981 ggcgggattt catttgcgcc attgctgatc cccatgtcta ccactggcag accgtgatgg
     8041 acgacaccgt gtccgccagc gtagctcaag ccctggacga actgatgctg tgggccgaag
     8101 actgtcccga ggtgcgccac ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg
     8161 acaacggccg catcaccgcc gtaatcgact ggtccgaagc tatgttcggg gacagtcagt
     8221 acgaggtggc caacatcttc ttctggcggc cctggctggc ttgcatggag cagcagactc
     8281 gctacttcga gcgccggcat cccgagctgg ccggcagccc tcgtctgcga gcctacatgc
     8341 tgcgcatcgg cctggatcag ctctaccaga gcctcgtgga cggcaacttc gacgatgctg
     8401 cctgggctca aggccgctgc gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca
     8461 cacaaatcgc tcgccggagc gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg
     8521 acagcggcaa ccgccggccc agtacacgac cgcgcgctaa ggaggtaggt cgagtttaga
     8581 ctctagccat cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     8641 ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt cccttgtcca
     8701 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     8761 cgtaatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     9061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     9121 caaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9301 cactgcggcc aacttacttc tgacaactat cggaggaccg aaggagctaa ccgctttttt
     9361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aattcgaaat
     9781 gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc
     9841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    10381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10501 agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10621 atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc tgggatggtg
    10681 aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc tgtaccctct
    10741 ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca gtttttcggg
    10801 tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt
    10861 gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag ccatcgagat
    10921 aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg atcgagatct tcaatattgg
//
