LOCUS       sequence-389049-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_C29X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_C29X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        482..685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     636..656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          821..953
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     936..955
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     997..1016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        997..1015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6119..6631
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6707..6725)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6762..6883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6773..6792)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6827..6846
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7128..7485
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7128..7319
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7151..7171)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7336..7471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7516..8553
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8532..8540
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8577..8625
                     /note="synthetic polyadenylation signal"
     promoter        8781..8885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8886..8954,8955..9746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9104..9123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9904..10492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9984..10003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10608..10891
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
       61 gtacatttat attggctcat gtccaatatg accgccatgt tggcattgat tattgactag
      121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      361 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      421 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      481 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      601 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      661 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcacta gaagctttat
      721 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt gcttctgaca caacagtctc
      781 gaacttaagc tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag
      841 acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt
      901 ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca
      961 ctcccagttc aattacagct cttaaggcta gagtattaat acgactcact atagggctag
     1021 caaagcgatc gccatgaaac tgcgtggagt cagcctggct gccggcttgt tcttactggc
     1081 cctgagtctt tgggggcagc ctgcagaggc tgcggcttga tatgggtgtt ctccaggatc
     1141 aaagtgtgac tgcagtggca taaaagggga aaagggagag agagggtttc caggtttgga
     1201 aggacaccca ggattgcctg gatttccagg tccagaaggg cctccggggc ctcggggaca
     1261 aaagggtgat gatggaattc cagggccacc aggaccaaaa ggaatcagag gtcctcctgg
     1321 acttcctgga tttccaggga caccaggtct tcctggaatg ccaggccacg atggggcccc
     1381 aggacctcaa ggtattcccg gatgcaatgg aaccaaggga gaacgtggat ttccaggcag
     1441 tcccggtttt cctggtttac agggtcctcc aggaccccct gggatcccag gtatgaaggg
     1501 tgaaccaggt agtataatta tgtcatcact gccaggacca aagggtaatc caggatatcc
     1561 aggtcctcct ggaatacaag gcctacctgg tcccactggt ataccagggc caattggtcc
     1621 cccaggacca ccaggtttga tgggccctcc tggtccacca ggacttccag gacctaaggg
     1681 gaatatgggc ttaaatttcc agggacccaa aggtgaaaaa ggtgagcaag gtcttcaggg
     1741 cccacctggg ccacctgggc agatcagtga acagaaaaga ccaattgatg tagagtttca
     1801 gaaaggagat cagggacttc ctggtgaccg agggcctcct ggacctccag ggatacgtgg
     1861 tcctccaggt cccccaggtg gtgagaaagg tgagaagggt gagcaaggag agccaggcaa
     1921 aagaggtaaa ccaggcaaag atggagaaaa tggccaacca ggaattcctg gtttgcctgg
     1981 tgatcctggt taccctggtg aacccggaag ggatggtgaa aagggccaaa aaggtgacac
     2041 tggcccacct ggacctcctg gacttgtaat tcctagacct gggactggta taactatagg
     2101 agaaaaagga aacattgggt tgcctgggtt gcctggagaa aaaggagagc gaggatttcc
     2161 tggaatacag ggtccacctg gccttcctgg acctccaggg gctgcagtta tgggtcctcc
     2221 tggccctcct ggatttcctg gagaaagggg tcagaaaggt gatgaaggac cacctggaat
     2281 ttccattcct ggacctcctg gacttgacgg acagcctggg gctcctgggc ttccagggcc
     2341 tcctggccct gctggccctc acattcctcc tagtgatgag atatgtgaac caggccctcc
     2401 aggcccccca ggatctccag gtgataaagg actccaagga gaacaaggag tgaaaggtga
     2461 caaaggtgac acttgcttca actgcattgg aactggtatt tcagggcctc caggtcaacc
     2521 tggtttgcca ggtctcccag gtcctccagg atctcttggt ttccctggac agaaagggga
     2581 aaaaggacaa gctggtgcaa ctggtcccaa aggattacca ggcattccag gagctccagg
     2641 tgctccaggc tttcctggat ctaaaggtga acctggtgat atcctcactt ttccaggaat
     2701 gaagggtgac aaaggagagt tgggttcccc tggagctcca gggcttcctg gtttacctgg
     2761 cactcctgga caggatggat tgccagggct tcctggcccg aaaggagagc ctggtggaat
     2821 tacttttaag ggtgaaagag gtccccctgg gaacccaggt ttaccaggcc tcccagggaa
     2881 tatagggcct atgggtcccc ctggtttcgg ccctccaggc ccagtaggtg aaaaaggcat
     2941 acaaggtgtg gcaggaaatc caggccagcc aggaatacca ggtcctaaag gggatccagg
     3001 tcagactata acccagccgg ggaagcctgg cttgcctggt aacccaggca gagatggtga
     3061 tgtaggtctt ccaggtgacc ctggacttcc agggcaacca ggcttgccag ggatacctgg
     3121 tagcaaagga gaaccaggta tccctggaat tgggcttcct ggaccacctg gtcccaaagg
     3181 ctttcctgga attccaggac ctccaggagc acctgggaca cctggaagaa ttggtctaga
     3241 aggccctcct gggccacccg gctttccagg accaaagggt gaaccaggat ttgcattacc
     3301 tgggccacct gggccaccag gacttccagg tttcaaagga gcacttggtc caaaaggtga
     3361 tcgtggtttc ccaggacctc cgggtcctcc aggacgcact ggcttagatg ggctccctgg
     3421 accaaaaggt gatgttggac caaatggaca acctggacca atgggacctc ctgggctgcc
     3481 aggaataggt gttcagggac caccaggacc accagggatt cctgggccaa taggtcaacc
     3541 tggtttacat ggaataccag gagagaaggg ggatccagga cctcctggac ttgatgttcc
     3601 aggaccccca ggtgaaagag gcagtccagg gatccccgga gcacctggtc ctataggacc
     3661 tccaggatca ccagggcttc caggaaaagc aggtgcctct ggatttccag gtaccaaagg
     3721 tgaaatgggt atgatgggac ctccaggccc accaggacct ttgggaattc ctggcaggag
     3781 tggtgtacct ggtcttaaag gtgatgatgg cttgcagggt cagccaggac ttcctggccc
     3841 tacaggagaa aaaggtagta aaggagagcc tggccttcca ggccctcctg gaccaatgga
     3901 tccaaatctt ctgggctcaa aaggagagaa gggggaacct ggcttaccag gtatacctgg
     3961 agtttcaggg ccaaaaggtt atcagggttt gcctggagac ccagggcaac ctggactgag
     4021 tggacaacct ggattaccag gaccaccagg tcccaaaggt aaccctggtc tccctggaca
     4081 gccaggtctt ataggacctc ctggacttaa aggaaccatc ggtgatatgg gttttccagg
     4141 gcctcagggt gtggaagggc ctcctggacc ttctggagtt cctggacaac ctggctcccc
     4201 aggattacct ggacagaaag gcgacaaagg tgatcctggt atttcaagca ttggtcttcc
     4261 aggtcttcct ggtccaaagg gtgagcctgg tctgcctgga tacccaggga accctggtat
     4321 caaaggttct gtgggagatc ctggtttgcc cggattacca ggaacccctg gagcaaaagg
     4381 acaaccaggc cttcctggat tcccaggaac cccaggccct cctggaccaa aaggtattag
     4441 tggccctcct gggaaccccg gccttccagg agaacctggt cctgtaggtg gtggaggtca
     4501 tcctgggcaa ccagggcctc caggcgaaaa aggcaaaccc ggtcaagatg gtattcctgg
     4561 accagctgga cagaagggtg aaccaggtca accaggcttt ggaaacccag gaccgcctgg
     4621 acttccagga ctttctggcc aaaagggtga tggaggatta cctgggattc caggaaatcc
     4681 tggccttcca ggtccaaagg gcgaaccagg ctttcacggt ttccctggtg tgcagggtcc
     4741 cccaggccct cctggttctc cgggtccagc tctggaagga cctaaaggca accctgggcc
     4801 ccaaggtcct cctgggagac caggtcctac aggttttcaa ggtctaccag gtccagaagg
     4861 tcctccaggt ctccctggaa atggaggtat taaaggagag aagggaaatc caggccaacc
     4921 tgggctacct ggcttgcctg gtttgaaagg agatcaagga ccaccaggac tccagggtaa
     4981 tcctggccgg ccgggtctca atggaatgaa aggagatcct ggtctccctg gtgttccagg
     5041 attcccaggc atgaaaggac ccagtggagt acctggatca gctggccctg agggggaacc
     5101 gggacttatt ggtcctccag gtcctcctgg attacctggt ccttcaggac agagtatcat
     5161 aattaaagga gatgctggtc ctccaggaat ccctggccag cctgggctaa agggtctacc
     5221 aggaccccaa ggacctcaag gcttaccagg tccaactggc cctccaggag atcctggacg
     5281 caatggactc cctggctttg atggtgcagg agggcgcaaa ggagacccag gtctgccagg
     5341 acagccaggt acccgtggtt tggatggtcc ccctggtcca gatggattgc aaggtccccc
     5401 aggtccccct ggaacctcct ctgttgcaca tggatttctt attacacgcc acagccagac
     5461 aacggatgca ccacaatgcc cacagggaac acttcaggtc tatgaaggct tttctctcct
     5521 gtatgtacaa ggaaataaaa gagcccacgg tcaagacttg gggacggctg gcagctgcct
     5581 tcgtcgcttt agtaccatgc ctttcatgtt ctgcaacatc aataatgttt gcaactttgc
     5641 ttcaagaaat gactattctt actggctctc taccccagag cccatgccaa tgagcatgca
     5701 acccctaaag ggccagagca tccagccatt cattagtcga tgtgcagtat gtgaagctcc
     5761 agctgtggtg atcgcagttc acagtcagac gatccagatt ccccattgtc ctcagggatg
     5821 ggattctctg tggattggtt attccttcat gatgcataca agtgcagggg cagaaggctc
     5881 aggtcaagcc ctagcctccc ctggttcctg cttggaagag tttcgttcag ctcccttcat
     5941 cgaatgtcat gggaggggta cctgtaacta ctatgccaac tcctacagct tttggctggc
     6001 aactgtagat gtgtcagaca tgttcagtaa acctcagtca gaaacgctga aagcaggaga
     6061 cttgaggaca cgaattagcc gatgtcaagt gtgcatgaag aggacattct cgagcggcgt
     6121 cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca acctggacca
     6181 agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc
     6241 gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat
     6301 cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt
     6361 gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac tggtaatcga
     6421 cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca tcgccgtgtt
     6481 cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa ttatcgacga
     6541 gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg gagtgaccgg
     6601 ctggcggctg tgcgaacgca ttctggcgta aggccgcgac tctagggcat gcaagctgat
     6661 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     6721 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     6781 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6841 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6901 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
     6961 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     7021 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     7081 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     7141 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7201 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7441 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cgattcttct
     7501 gacactagcg ccaccatgaa gaagcccgaa ctcaccgcta ccagcgttga aaaatttctc
     7561 atcgagaagt tcgacagtgt gagcgacctg atgcagttgt cggagggcga agagagccga
     7621 gccttcagct tcgatgtcgg cggacgcggc tatgtactgc gggtgaatag ctgcgctgat
     7681 ggcttctaca aagaccgcta cgtgtaccgc cacttcgcca gcgctgcact acccatcccc
     7741 gaagtgttgg acatcggcga gttcagcgag agcctgacat actgcatcag tagacgcgcc
     7801 caaggcgtta ctctccaaga cctccccgaa acagagctgc ctgctgtgtt acagcctgtc
     7861 gccgaagcta tggatgctat tgccgccgcc gacctcagtc aaaccagcgg cttcggccca
     7921 ttcgggcccc aaggcatcgg ccagtacaca acctggcggg atttcatttg cgccattgct
     7981 gatccccatg tctaccactg gcagaccgtg atggacgaca ccgtgtccgc cagcgtagct
     8041 caagccctgg acgaactgat gctgtgggcc gaagactgtc ccgaggtgcg ccacctcgtc
     8101 catgccgact tcggcagcaa caacgtcctg accgacaacg gccgcatcac cgccgtaatc
     8161 gactggtccg aagctatgtt cggggacagt cagtacgagg tggccaacat cttcttctgg
     8221 cggccctggc tggcttgcat ggagcagcag actcgctact tcgagcgccg gcatcccgag
     8281 ctggccggca gccctcgtct gcgagcctac atgctgcgca tcggcctgga tcagctctac
     8341 cagagcctcg tggacggcaa cttcgacgat gctgcctggg ctcaaggccg ctgcgatgcc
     8401 atcgtccgca gcggggccgg caccgtcggt cgcacacaaa tcgctcgccg gagcgcagcc
     8461 gtatggaccg acggctgcgt cgaggtgctg gccgacagcg gcaaccgccg gcccagtaca
     8521 cgaccgcgcg ctaaggaggt aggtcgagtt tagactctag ccatcacgat ggccgcaata
     8581 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa
     8641 ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     8701 ggggtcagca ccgtttctgc ggactggctt tctacgtaat ggtttcttag acgtcaggtg
     8761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9061 gccccgaaga acgttttcca atgatgagca ctttcaaagt tctgctatgt ggcgcggtat
     9121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9301 ctatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9721 gtgcctcact gattaagcat tggtaattcg aaatgaccga ccaagcgacg cccaaccggt
     9781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10321 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    10381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc
    10501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg
    10621 aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt
    10681 ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa
    10741 aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct
    10801 gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa
    10861 tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc
    10921 gtccggcgta gaggatcgag atcttcaata ttggccatta gccatattat tcattggtta
//
