LOCUS       sequence-389050-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_G5X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_G5X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        117..496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        497..700
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     651..671
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          836..968
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     951..970
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1012..1031
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1012..1030
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6134..6646
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6722..6740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6777..6898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6788..6807)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6842..6861
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7143..7500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7143..7334
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7166..7186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7351..7486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7531..8568
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8547..8555
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8592..8640
                     /note="synthetic polyadenylation signal"
     promoter        8796..8900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8901..8969,8970..9761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9119..9138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9919..10507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9999..10018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10623..10906
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
       61 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca
      121 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
      541 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      601 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
      661 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      721 cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgcttc
      781 tgacacaaca gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag
      841 tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag
      901 aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct
      961 ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta ttaatacgac
     1021 tcactatagg gctagcaaag cgatcgccat gaaactgcgt tgagtcagcc tggctgccgg
     1081 cttgttctta ctggccctga gtctttgggg gcagcctgca gaggctgcgg cttgctatgg
     1141 gtgttctcca ggatcaaagt gtgactgcag tggcataaaa ggggaaaagg gagagagagg
     1201 gtttccaggt ttggaaggac acccaggatt gcctggattt ccaggtccag aagggcctcc
     1261 ggggcctcgg ggacaaaagg gtgatgatgg aattccaggg ccaccaggac caaaaggaat
     1321 cagaggtcct cctggacttc ctggatttcc agggacacca ggtcttcctg gaatgccagg
     1381 ccacgatggg gccccaggac ctcaaggtat tcccggatgc aatggaacca agggagaacg
     1441 tggatttcca ggcagtcccg gttttcctgg tttacagggt cctccaggac cccctgggat
     1501 cccaggtatg aagggtgaac caggtagtat aattatgtca tcactgccag gaccaaaggg
     1561 taatccagga tatccaggtc ctcctggaat acaaggccta cctggtccca ctggtatacc
     1621 agggccaatt ggtcccccag gaccaccagg tttgatgggc cctcctggtc caccaggact
     1681 tccaggacct aaggggaata tgggcttaaa tttccaggga cccaaaggtg aaaaaggtga
     1741 gcaaggtctt cagggcccac ctgggccacc tgggcagatc agtgaacaga aaagaccaat
     1801 tgatgtagag tttcagaaag gagatcaggg acttcctggt gaccgagggc ctcctggacc
     1861 tccagggata cgtggtcctc caggtccccc aggtggtgag aaaggtgaga agggtgagca
     1921 aggagagcca ggcaaaagag gtaaaccagg caaagatgga gaaaatggcc aaccaggaat
     1981 tcctggtttg cctggtgatc ctggttaccc tggtgaaccc ggaagggatg gtgaaaaggg
     2041 ccaaaaaggt gacactggcc cacctggacc tcctggactt gtaattccta gacctgggac
     2101 tggtataact ataggagaaa aaggaaacat tgggttgcct gggttgcctg gagaaaaagg
     2161 agagcgagga tttcctggaa tacagggtcc acctggcctt cctggacctc caggggctgc
     2221 agttatgggt cctcctggcc ctcctggatt tcctggagaa aggggtcaga aaggtgatga
     2281 aggaccacct ggaatttcca ttcctggacc tcctggactt gacggacagc ctggggctcc
     2341 tgggcttcca gggcctcctg gccctgctgg ccctcacatt cctcctagtg atgagatatg
     2401 tgaaccaggc cctccaggcc ccccaggatc tccaggtgat aaaggactcc aaggagaaca
     2461 aggagtgaaa ggtgacaaag gtgacacttg cttcaactgc attggaactg gtatttcagg
     2521 gcctccaggt caacctggtt tgccaggtct cccaggtcct ccaggatctc ttggtttccc
     2581 tggacagaaa ggggaaaaag gacaagctgg tgcaactggt cccaaaggat taccaggcat
     2641 tccaggagct ccaggtgctc caggctttcc tggatctaaa ggtgaacctg gtgatatcct
     2701 cacttttcca ggaatgaagg gtgacaaagg agagttgggt tcccctggag ctccagggct
     2761 tcctggttta cctggcactc ctggacagga tggattgcca gggcttcctg gcccgaaagg
     2821 agagcctggt ggaattactt ttaagggtga aagaggtccc cctgggaacc caggtttacc
     2881 aggcctccca gggaatatag ggcctatggg tccccctggt ttcggccctc caggcccagt
     2941 aggtgaaaaa ggcatacaag gtgtggcagg aaatccaggc cagccaggaa taccaggtcc
     3001 taaaggggat ccaggtcaga ctataaccca gccggggaag cctggcttgc ctggtaaccc
     3061 aggcagagat ggtgatgtag gtcttccagg tgaccctgga cttccagggc aaccaggctt
     3121 gccagggata cctggtagca aaggagaacc aggtatccct ggaattgggc ttcctggacc
     3181 acctggtccc aaaggctttc ctggaattcc aggacctcca ggagcacctg ggacacctgg
     3241 aagaattggt ctagaaggcc ctcctgggcc acccggcttt ccaggaccaa agggtgaacc
     3301 aggatttgca ttacctgggc cacctgggcc accaggactt ccaggtttca aaggagcact
     3361 tggtccaaaa ggtgatcgtg gtttcccagg acctccgggt cctccaggac gcactggctt
     3421 agatgggctc cctggaccaa aaggtgatgt tggaccaaat ggacaacctg gaccaatggg
     3481 acctcctggg ctgccaggaa taggtgttca gggaccacca ggaccaccag ggattcctgg
     3541 gccaataggt caacctggtt tacatggaat accaggagag aagggggatc caggacctcc
     3601 tggacttgat gttccaggac ccccaggtga aagaggcagt ccagggatcc ccggagcacc
     3661 tggtcctata ggacctccag gatcaccagg gcttccagga aaagcaggtg cctctggatt
     3721 tccaggtacc aaaggtgaaa tgggtatgat gggacctcca ggcccaccag gacctttggg
     3781 aattcctggc aggagtggtg tacctggtct taaaggtgat gatggcttgc agggtcagcc
     3841 aggacttcct ggccctacag gagaaaaagg tagtaaagga gagcctggcc ttccaggccc
     3901 tcctggacca atggatccaa atcttctggg ctcaaaagga gagaaggggg aacctggctt
     3961 accaggtata cctggagttt cagggccaaa aggttatcag ggtttgcctg gagacccagg
     4021 gcaacctgga ctgagtggac aacctggatt accaggacca ccaggtccca aaggtaaccc
     4081 tggtctccct ggacagccag gtcttatagg acctcctgga cttaaaggaa ccatcggtga
     4141 tatgggtttt ccagggcctc agggtgtgga agggcctcct ggaccttctg gagttcctgg
     4201 acaacctggc tccccaggat tacctggaca gaaaggcgac aaaggtgatc ctggtatttc
     4261 aagcattggt cttccaggtc ttcctggtcc aaagggtgag cctggtctgc ctggataccc
     4321 agggaaccct ggtatcaaag gttctgtggg agatcctggt ttgcccggat taccaggaac
     4381 ccctggagca aaaggacaac caggccttcc tggattccca ggaaccccag gccctcctgg
     4441 accaaaaggt attagtggcc ctcctgggaa ccccggcctt ccaggagaac ctggtcctgt
     4501 aggtggtgga ggtcatcctg ggcaaccagg gcctccaggc gaaaaaggca aacccggtca
     4561 agatggtatt cctggaccag ctggacagaa gggtgaacca ggtcaaccag gctttggaaa
     4621 cccaggaccg cctggacttc caggactttc tggccaaaag ggtgatggag gattacctgg
     4681 gattccagga aatcctggcc ttccaggtcc aaagggcgaa ccaggctttc acggtttccc
     4741 tggtgtgcag ggtcccccag gccctcctgg ttctccgggt ccagctctgg aaggacctaa
     4801 aggcaaccct gggccccaag gtcctcctgg gagaccaggt cctacaggtt ttcaaggtct
     4861 accaggtcca gaaggtcctc caggtctccc tggaaatgga ggtattaaag gagagaaggg
     4921 aaatccaggc caacctgggc tacctggctt gcctggtttg aaaggagatc aaggaccacc
     4981 aggactccag ggtaatcctg gccggccggg tctcaatgga atgaaaggag atcctggtct
     5041 ccctggtgtt ccaggattcc caggcatgaa aggacccagt ggagtacctg gatcagctgg
     5101 ccctgagggg gaaccgggac ttattggtcc tccaggtcct cctggattac ctggtccttc
     5161 aggacagagt atcataatta aaggagatgc tggtcctcca ggaatccctg gccagcctgg
     5221 gctaaagggt ctaccaggac cccaaggacc tcaaggctta ccaggtccaa ctggccctcc
     5281 aggagatcct ggacgcaatg gactccctgg ctttgatggt gcaggagggc gcaaaggaga
     5341 cccaggtctg ccaggacagc caggtacccg tggtttggat ggtccccctg gtccagatgg
     5401 attgcaaggt cccccaggtc cccctggaac ctcctctgtt gcacatggat ttcttattac
     5461 acgccacagc cagacaacgg atgcaccaca atgcccacag ggaacacttc aggtctatga
     5521 aggcttttct ctcctgtatg tacaaggaaa taaaagagcc cacggtcaag acttggggac
     5581 ggctggcagc tgccttcgtc gctttagtac catgcctttc atgttctgca acatcaataa
     5641 tgtttgcaac tttgcttcaa gaaatgacta ttcttactgg ctctctaccc cagagcccat
     5701 gccaatgagc atgcaacccc taaagggcca gagcatccag ccattcatta gtcgatgtgc
     5761 agtatgtgaa gctccagctg tggtgatcgc agttcacagt cagacgatcc agattcccca
     5821 ttgtcctcag ggatgggatt ctctgtggat tggttattcc ttcatgatgc atacaagtgc
     5881 aggggcagaa ggctcaggtc aagccctagc ctcccctggt tcctgcttgg aagagtttcg
     5941 ttcagctccc ttcatcgaat gtcatgggag gggtacctgt aactactatg ccaactccta
     6001 cagcttttgg ctggcaactg tagatgtgtc agacatgttc agtaaacctc agtcagaaac
     6061 gctgaaagca ggagacttga ggacacgaat tagccgatgt caagtgtgca tgaagaggac
     6121 attctcgagc ggcgtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     6181 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     6241 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     6301 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     6361 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     6421 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     6481 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     6541 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     6601 caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaaggcc gcgactctag
     6661 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     6721 accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag
     6781 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6841 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6901 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     6961 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     7021 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     7081 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     7141 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7201 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7321 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7381 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7441 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7501 aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac cgctaccagc
     7561 gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca gttgtcggag
     7621 ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt actgcgggtg
     7681 aatagctgcg ctgatggctt ctacaaagac cgctacgtgt accgccactt cgccagcgct
     7741 gcactaccca tccccgaagt gttggacatc ggcgagttca gcgagagcct gacatactgc
     7801 atcagtagac gcgcccaagg cgttactctc caagacctcc ccgaaacaga gctgcctgct
     7861 gtgttacagc ctgtcgccga agctatggat gctattgccg ccgccgacct cagtcaaacc
     7921 agcggcttcg gcccattcgg gccccaaggc atcggccagt acacaacctg gcgggatttc
     7981 atttgcgcca ttgctgatcc ccatgtctac cactggcaga ccgtgatgga cgacaccgtg
     8041 tccgccagcg tagctcaagc cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag
     8101 gtgcgccacc tcgtccatgc cgacttcggc agcaacaacg tcctgaccga caacggccgc
     8161 atcaccgccg taatcgactg gtccgaagct atgttcgggg acagtcagta cgaggtggcc
     8221 aacatcttct tctggcggcc ctggctggct tgcatggagc agcagactcg ctacttcgag
     8281 cgccggcatc ccgagctggc cggcagccct cgtctgcgag cctacatgct gcgcatcggc
     8341 ctggatcagc tctaccagag cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa
     8401 ggccgctgcg atgccatcgt ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct
     8461 cgccggagcg cagccgtatg gaccgacggc tgcgtcgagg tgctggccga cagcggcaac
     8521 cgccggccca gtacacgacc gcgcgctaag gaggtaggtc gagtttagac tctagccatc
     8581 acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     8641 aatcgatagc gataaggatc ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     8701 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtaatggttt
     8761 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8821 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8881 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8941 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9001 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9061 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc aaagttctgc
     9121 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9181 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9241 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9301 acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9361 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9421 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9481 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9541 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9601 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9661 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9721 agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg accgaccaag
     9781 cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9901 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10321 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10501 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
    10621 gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa
    10681 aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg gttgcatagg
    10741 tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt ggtttgttgc
    10801 catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta
    10861 agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta
    10921 ctccggccac gatgcgtccg gcgtagagga tcgagatctt caatattggc cattagccat
//
