LOCUS       sequence-389069-       19446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Hunkeler M, Jin CY, Fischer ES
  TITLE     Structures of BIRC6-client complexes provide a mechanism of 
            SMAC-mediated release of caspases.
  JOURNAL   Science. 2023 Mar 17;379(6637):1105-1111. doi: 
            10.1126/science.ade5750. Epub 2023 Feb 9.
  PUBMED    36758104
REFERENCE   2  (bases 1 to 19446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade5750"; journalName: "Science"; date: 
            "2023-03-17- 17"; volume: "379"; issue: "6637"; pages: "1105-1111"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut
FEATURES             Location/Qualifiers
     source          1..19446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(67..600)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(789..794,795..811,812..817))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    865..1072
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1061..1078
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             1145..1156
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1554..1574
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16116..16139)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(16158..16223)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      16226..16235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(16288..16307)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(16809..17012)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(16838..16858)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        17013..17316
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     mobile_element  17410..17575
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        17600..17671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17672..17740,17741..18532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17890..17909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18571..19159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     19060..19079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  19223..19446
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
       61 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
      121 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
      181 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
      241 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
      301 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
      361 agcaagcgca ataaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
      421 atgttgggag taggtggcta catcaccgaa ctcacgaccg aaaagatcaa gagcagcccg
      481 catggatttg acttggtcag ggccgagcct acatgtacga atgatgccca tacttgagcc
      541 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat
      601 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
      661 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
      721 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
      781 tgcattacag tttacgaacc gaacaggctt atgtcaacga gtagccaccg ttcgggcgtc
      841 ccagcgattc gcccttaact cgagtcccca gcatgcctgc tattgtctgc ccaatcctcc
      901 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaattt
      961 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     1021 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggca
     1081 gatctcgaag ccgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     1141 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     1201 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
     1261 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcactg agttccgccg
     1321 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     1381 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     1441 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     1501 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     1561 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     1621 gttgatttta cggccacagg ccattacggc cacaggccat tatcatcagc ggccgctcaa
     1681 agctgaaagt cggacggaag ttcctttgaa cttgagggtt ttacctgatc gtgcataagg
     1741 gtctcttccg cacctgtagt ggctctacat acttctgggg cgtcgtccgt gtcaggatcc
     1801 aaaccctcag gacacggcaa tttcaagagt tcctcgcgga gttgggccgt atgtcgtttg
     1861 agagccgctg cgtgatgaga catagtccgt ccgacccgtt tgtctgagga atattgttgg
     1921 atgtctgcta tccactcttc acactgcgcc ataatttcta ctcttttcaa atagaaatgt
     1981 ttatgtatca cctccttgaa gcaaggagag ggattcctaa tctgttcgag catagcccac
     2041 ttcactgtag cttggcggat attgccatcg tactccctgc tggattgagt accagatggt
     2101 gtgcctcgag acctctcgta accaggctcg ttaaagtaag gttccgccac gaggatcaat
     2161 gattgaacgg acacgagtac ctggagaaat gatgatgttt gcgggttcca tttttcttcg
     2221 ggtcttccat gccatgtatt cagaattgag aggcaaactt tgccgtcgtt atacaggttg
     2281 gggttgaagc gtacggagtg acccccggta gtttccaaat tgacaagggg cgggctggaa
     2341 gggtaatcct gtgggaagta aacgtcaaac tcgaagcatc cgtttgcata aggagtgtct
     2401 gcggggccgg tgatcagaac tttcataata tccaatctct cttcatcaca tctcacgaag
     2461 actgaactac tggatgacaa ggggagacta gtactgaggg taaccgcttc ttgagcaagg
     2521 cgccgagccc tagctgcact atttgcatcg ttagcgtttt tgacctggct catataatga
     2581 tagttcactt tgaatcccag cttgccgtcc tcgtcttcag acaccatttc aaaagtgtcg
     2641 aattggagct ttttcatcac tgcgacatac ttttcctcaa gggatttcag gacggacagc
     2701 ggcttaggct tcatggccgc tttctttgaa tactccccaa gtttcttctc ctggttggcc
     2761 tgtcggagtg atgttgttgc agcgtaaaca atttcagctg ttttctgtat atctggtacg
     2821 agcaaagtaa gcccttcggg ttcctggtca gatgcgtctg gcttaactcc ggttttaaca
     2881 ttctctctct tagaccgcag gcggttggta taagtatcga cacaagtttt cattttggca
     2941 aggagagtgc caacactggt ctggcactcg ctctgctcct cctcctcctc gccattctcg
     3001 gtgctcaggg ggagcaggag aggcaccatg gcagcgcaac tggctatagc tctcagcagt
     3061 tccagaagag ctctatacaa gggaacatgt ctcgccatgt ccagcacaga gtcgtttcga
     3121 aggtatgaac tcatggcagg tatcaaacaa ctctggctaa gaagctccag gagcacactg
     3181 gggagagcat tggaattctg accgcgcgtt tcgtgactgg attgtgcctc gccattcacc
     3241 gctgaagaca cagggtttat atagctcgca agcacctgga gcagacaagt tacatgttcc
     3301 tcctccaacc tttgcttcgt aagagcttgt tccacatccc atccggaggc ggttgagcca
     3361 gtcccaaatc cggtcccttt tgcccagtag agctgctgct cctcggtact cgtggggttg
     3421 tgggagctgc tcacctgtgg ttccgtctgg ttcacgctgc tgtttgggac ccggggactg
     3481 tggtggctca acgcactgag gcacacgaga atgaggtgca aggccccgat ctcaagggcc
     3541 attcgtcgca gaaggacgcc gtcatcagtt gtgagggggg tagttgaaaa gagggatcgg
     3601 agcacatgaa aaggaagtgt gggcaatacg ttcgcgctgg ggctctgaag gatatgggaa
     3661 ccttctccat cgtccgtcac ccccaaaacg agccgcagaa ggcattgcgc atgcttgcgt
     3721 tctttcagca ggacttcagc gtaaccaggg agcctaagaa aaagcccaaa tgccgccaaa
     3781 gagtgcgctg gtattggaga cattgtctgg acggcggatt ttattggggg cggctcggca
     3841 gcaaccgttt cgggggcctc atacaccaat tcgccgctct gctcaattgt cacccattca
     3901 aattgatctg aatctttagg tttctcttgg gtggttgagg ttacaaccgg agctgatacc
     3961 tgctggatca cttcaggata aagcatgggc aacctctcgg cgatgagagc aagaccaccc
     4021 atcccggcga atacctggag cggggattgt atcggacagg tttccagcag gctttcatcc
     4081 aaaattccgt ccatgcattc gtcgccgggt gtcaaggcct cgtcctctgg gcaggaagca
     4141 aggaggtcac cgtgatcctt ttcagggtgc aagcgcttat aggaatcagt tttggacagt
     4201 gaagggtctg taatcacccg actgcccagc ttaaccgtaa gatctataga tctgtacccc
     4261 tgaggcaact tgcggtcata aagcagggtg agcagttgag ccagagtcat ttccgctggc
     4321 agtggctgac ctgcgagaag cttatggaac agatgaaaca ctggtaccgt gatgatttta
     4381 ttcgctgcct cggcgccgct cgtgcttccg atcttgtccg gtatcgtcct cccgcggcgt
     4441 gatggtggtc gaggaggggt cgcgctgacc gccctcttgc tttgactctt gagcccggag
     4501 gcgactacca cggagtagtt atcctgaaag ccattttcgg ctttgacttt ctctttttcc
     4561 tcgtggtttt ttttttcttt gtcatctttc tgctcggaga taagcttggc ggtgatgctc
     4621 ggagtatcgg agaccctatc aaggacatct gaaagcgtcg tagatactgg caggtggagt
     4681 gttctaaatt tgtgcccggc cccgtacatc gggtgctgaa tgacatggct cgtggcattt
     4741 atccttccct tatagagcgg gcacgggctc tgcaggaaca tagtgacctt ttcgtcctcc
     4801 agcatgagtt gaaggaagag tcgtctgata aaaccgctta tgttgccaga cgtcgggaga
     4861 ttgcccctct gtggactggt ctgaaagagc tcgcaaagca cttgagccat aagcttttga
     4921 ttatttgggt ggcaacttat gcactggaga aaaaacgcca ctgtcgcgtt ttctatagcg
     4981 gttctttgtt gtgttgtaag acccgtagta gcggcagaac tcaagctggc agacctagcg
     5041 ctcgtctgct gggccccgag attgtgtgag ccgctggtac tcccagaatg acacaacaag
     5101 aacagcagtg cagtccagag aggattgact tcggaaccac ccagccagtc tttcatgatg
     5161 tggctgttac cgacctcggt aaggaaccgc aatatcgggg cgacgagatc cgccgtaatc
     5221 ggcattttgt tacattgctg gggaacatga tgccgccgga gtttatcctg tgaaatgtca
     5281 atgctctgtg caatggattc actactactt atgtgggaaa aacagaatga agccaagctc
     5341 cgaacaagga ggctcggcag cccgctgtcc aggagctgct taatagcttc aggagactgt
     5401 gaagaacttg cgagggtagc caagtggctt tcagtcagcg ccaagggtgc ttgggcattt
     5461 ttagaatcat ctgtcagaga tgaagacaag tcttgctttt tgctgtcatc tgtcatggac
     5521 aagcggtgat ggcattgaac cggcgggggg gttatgccca tccaacccat caacagagag
     5581 aagtagttag gatgtacgtg acacatagag caaagcaagc tgtcaacagc ctttttgaga
     5641 accatattac acggcagaga catggaccaa ttaaacagaa gctcaaagag ctcctgatcc
     5701 agcagtgctg gcaagtcgta ttcaacgagc aactcatagg agtgccacag aattgccgca
     5761 acgcaatgca ggtgactcgg tgacggcatc agaagcccgt attctacagt gctggaattc
     5821 gggcacatgt aattcattcg gccagatctt ttcatagctg ctacgcgcgc gctatcgctt
     5881 acccatttga gcaatgcctg aatccgatct ttggttcccg gatcttgggt agttccaagt
     5941 tgatacaaaa tgtctattgt actatcgcta gagaggccat tcaaccgacc aaacaacagt
     6001 ggactgttca gatctgtagg atcagaacca ctagctgcac aattccgcaa caagatgtct
     6061 ataagcttga gacctatccg agttgactgg aggccgattt taaccaagac gacctcaata
     6121 ttgggtgagt gcattccgca atagggggac atgagaagtg cggcgcaagt ctggagaaga
     6181 ttggccgtgg gggcagccgc tgaagccatc attccttcca gatctgagat atgcgtgaga
     6241 cagtgatgga ggagccggag ccatccgata gatgttttgc taacttgatc ttcggaaggc
     6301 aaaaacggat tatttaccgt agcagaagat gtggtcccaa acgctgtaag ccccaggagt
     6361 ttaatctggg aaagcccgag cgtggaagcg tcgcgggggc ggtgcaagcg caagcagacc
     6421 gcggaagcta cctcagcttt tacaagttgg atctttatgt aggtaagtcc tgaagttacg
     6481 accggagtac tgagcgggag catatttact ccatccgcac taacctcgac tgatacagag
     6541 gatggacacg tagccaaaga agccaggtga ggctgaatat ggatttcctt aaggagcacc
     6601 gcggcaggga gatgaatagt gagatcgcac catgcttcct ctggcaagaa tatatatgac
     6661 caagctgcgg atctggccct ccggtgagga ggagtggctt gaagcaggac ttccgccggc
     6721 tgtgccgttg gagaagagct cgtaattgta cccaaaggtg caaaattgtg tattccttca
     6781 gcattatctg gctcagaggc aaggatcctc gtcttcaagg tagaatcgac tgccttgttc
     6841 ggcccagagt caaggaactt cataatggtg gagaccatag atcttgctgt gttgacaatc
     6901 gctctagtgc ttgtaaccat gttattgcag atgagctgca ctcctcccat gtcatggaag
     6961 gctttaagcg cgtcagaagt atcgaggact cggagtatga accacagcag aggctcagac
     7021 aactggcatg tggcaagtga cccctgccgg cccatgtatc cacatgccat atatgtcaag
     7081 atcggctgca gcatcatgtg aaccgtagac gctttcttgc tcaaagtagt cagaatcgtg
     7141 aaaagtccat ctcccacaga gatcgcatcc aaaccgccat gaaagttttc atgcttagtc
     7201 aaatgcaacc cagaagctcc tataatcatg gccattgcag aggaattgca caagccgagt
     7261 gcggacagaa tttgactcat tatctgttga ttggcgagca cgagatccgc cacgtaggct
     7321 ggggatcctt gtacgctggt gctggaacca tttccatctt tacccccgga cactttttct
     7381 tcgtcggaaa cggaatccgt cgtattagtt gtgacgctta ctctagcact gtattcccta
     7441 ttgccactca aaggcagttg cacaaggatg ttgacgagct tcagaagatc gaacatcaac
     7501 tgatctctgg tcatctctcc gcacactgct tttgcgtctc caggtcggat cagattagcg
     7561 aagagaccac caaaacaagc ccgagcgccc acactactgt ctgagccact tcttgacatg
     7621 accttatctg agcatgtgat ataatggtgt acaaggaacg taacgctttc gatgacagca
     7681 gatatagtag agacacttac aacctcgaag ttttgttcaa gggtgaactc caacagcttg
     7741 acttgagcat ccaaaggacc ttgccccgtc tggaacgccc ctctttcggt actgaggata
     7801 tgaataagct tgaggaggaa ttctgagaat atctgagggc aggttgaaga aaggtgtacc
     7861 tggagtctgg tcaaaaactc ctgcatcgcc tgggtggctg taggtccaac ttgtgggctg
     7921 tgttggttcg taccatgcgg ggaagtacca gagaggaatt taaccagtac gtggatcagg
     7981 tttggatctg acaccaacag gtgtgccgta gattcctgag ttcctatagc gctggaacta
     8041 cctgaattca attgcatatt tgtcatcagg gtcagactat gcaaaaccaa gcaccaggtg
     8101 cgcaaaagtg tgttgggata ccacgccagc acggtgagga agctggtgat agaggcttga
     8161 ttaagactaa tcaccggtat tctattggca ttgtacagaa agatatctgc accattttcc
     8221 ttattgcctg tagaggaact attcagggaa agtgtaagcc acagttggag caaggactca
     8281 agctgcacgt ggtgaacctg gagcatggag aaaagtttgt tgaaacaaac gttcagcata
     8341 ggtacagatg agagctgtat aatcagctgg ctggtttgat tcgcagcttg aagcccacca
     8401 aggagtgaat catcggtccc agttgggctc tgagacaagg taggtgacgt attggtcaga
     8461 gcctgcaata tactgtccgc ttcaagagtg ctcatcattt tcaacatcaa ctctgcctgc
     8521 tgctccaaac ccacttccag gcgagcatcc agggcttgag aaacggacac ggatacagtc
     8581 tgactaccgt tcttctcagt attcgctgga ggtttcgcaa ctgggattcc aagtccacct
     8641 atgtaaggat tatagttata tgtaccgtag tccgcgcccc aataatcgta ccaagtagat
     8701 gagatgggtt tgaatacctt tgccgccaat gggtccttct ccaggtctat gtcgagtgga
     8761 tcgatgtcta cttccataag gtcctggaga agctcgagat ctatctcttt gtctttctca
     8821 agtgatgaca gggggggtgc accagtgatg tcgtgggtaa ggggttcgtc aatggtctcc
     8881 agaagctgaa ctgatgattg ttgcagacta tcatcggaat cccccgccgt agctccaaca
     8941 tcatcaccaa cggtcccttc ttccatcgct tcggcggcca ccggagcaag caactctcct
     9001 gcgagtatat cttggagcat acaagtcaat gagtattggg cccaggcgcc gccccagctg
     9061 atgtcgcctc ggttgaagtc cgtcccgacc aacaagagca gcaatctttc cagttggggt
     9121 tcatttacaa tttcgcacaa gtgtattgtt ggcctggtcg cattagcaat tcgtgccaag
     9181 accttacaaa cgaagagcaa gaggtcggca tgacatgtga aatccataga caacaagaaa
     9241 agaacaagct tttgaaccac gctaatgcac ctttcatggg ccaaagtgaa aggcagtggt
     9301 tcaatttccg gagactcctt ggccgtggta acttctgtat caagattact tctactccac
     9361 tcggccgtcc gtctcagcct aatgagatct ttaaaatgct tactcgttgc ttgcttaagt
     9421 ttggcctgct caacgagcaa cttatagcta gatcccttat ttgactgaat tttttctttc
     9481 tccatctgct caaccagagc cttttgtttc gccttgagga ggttaagctg ctgcttatga
     9541 tgaacgagtt gtttccggat ttttcttgag tacaatctat ccaggctact tgaccctttg
     9601 ctagagcggt tcaaggattg tgtatgaaga ttattgttta tgaaagacca ctgattgcca
     9661 ttcaaaggtt tttttgctgc ggctgcttca tcctctacgg ttgccacata gtcaagcaac
     9721 cgactgacca acattacgac ccatgatatc attgggatgt caagaacccc ttcagtccgt
     9781 cggtgcatgg ggagctgtgg ctgcaacggg gaaagcaaat tatccaagag gttcagcagg
     9841 gattcgagta caccgctatt agacaagctt cgctgtccaa tgcagctgag caacatgaac
     9901 acccggtctt gcggaaatat caaaagttgt tcagaattat aaagctcctg caaaacgttg
     9961 gaaagaaatt gaccccacca cctctccccg ccgcacaatt gcaccagcaa aagccctgtg
    10021 tgaagcctaa tctttggtgt ccctgaaata cacaaatgct tgaagagttc ctcacaagct
    10081 tgcgcatgga atgtgctctg caggactgct ggcacggaat gtccattgct tgcgtgcaac
    10141 aaggagagaa gcgtgtccag caagtaccgt atgatcgttc gcagttgttc tgtggagctt
    10201 gtctgtatca ggtcttcggg attacttgtt tcagagagca tctttcttga ggcccccaag
    10261 gcaaccttca ggcgttgaac agcattgtgg gcaaggttga gctgaagctg aagttgaata
    10321 cagtcctggt atgcagagaa gaccctagaa tcttcctcga cagctttgtc gggtttcctc
    10381 aggaagtact gcgcgttgtt agcgctattc aatggtggaa gatcaatgga ctggagcaag
    10441 gtttccaagc ggtgacaagc cagattgtag cgacactgaa tgtcttcttg caatgccacc
    10501 atcatggcaa ggtgctgatc aatctcgggc tgattggcag attccccttc gcctttcaat
    10561 gggtcatgca tcaacttaag ctcggactcc caaggaagaa tgtacgtgtg tccatagtaa
    10621 aaaccgagtg gtatcttcgc ccttgcattc gttgaaccat accggccgat gactgtaatt
    10681 ttcatgaagc gacatacggg gggcggaatg agatcatgca aaatcaggga gtgagtgcta
    10741 atgtctgttg ctacaaccag tctgcgaccg tcaacctctt cacccagggt ccatatatca
    10801 atggaaaggc tagccaagtc gccacaggtg ggtataagaa cgtctgtcag gaggattggc
    10861 cttccgaaat ccaacgtaac gaagcgtcta gctccactat gcatccgttc aattatgatg
    10921 gattgatggg gagggggctg aaggaagtgg ctagcgtgtg agatggcaag agcaagtcct
    10981 gaccccaaag ggttcattcg ggatttgttt gagtttttgt tgtgtgctgc gagattgacg
    11041 gcaggcgggt caataactga acccgggaaa agctgggcca actctgcgtt gatgacgaca
    11101 gaaacagctt catttggcgg cgtcaagggt ggagtcataa acaggggtgt cgtttttgga
    11161 gtaggcggga tcacatcgct tgggtgtata aagaagcccg gggcagcgac ggggttgcta
    11221 gggacgctat tgtgtacggg gccgacagct gagccggagc ccacctgtgc agcggaactc
    11281 agagccgttg atgcctcccc taccgttcct gaggagagcc ctcccaccgc cggtgtcaca
    11341 ccgaagctac tcatagcatc atctcgctca atccgcgtcc cgtcagatgt tgaaacacat
    11401 gtgaaatgga gcggctcgac ttcaagaagt ccggtaaggc tagtaaatga gccttcagcc
    11461 gccgtacaac ttgaaacctt accgtttcca ctgagtacgt cggacaaatc tatttcgtca
    11521 gactggaccc ctatgctgct gttatataca ttcttgcatg aagacccaga catctcaagg
    11581 tcgaacagca ctgggtcgaa cggagaggag tagagtccat atcgtgtctg gagaagcgct
    11641 tccatcggtg tcaagcgatc ctgcagttgg cgggagacag cagtcaaaag tgaggcacag
    11701 gccgtactgc agccagcaag atcttcatcc ttgacgtagt tcagaagaac aaaataccag
    11761 tacactgagc ccccagtcgt ggcagccggc agaaatgaca tgttatcgag gagggccttc
    11821 aaaaggactg gaccgaacgc gggttggcaa ggatcgcact taccgtttga gatacagagc
    11881 gcgagaaacc tagcgcattt gtgtgctata cttcgcccgg cttcgaaaaa gcagacgcgc
    11941 acaatatcac tcaggaattt ccgggttttt gacaggagat tttcgttacc ttccttggag
    12001 ctacctgggc cattggagtg tacccccgcc agccagcaga gtgtgtcaag gaccagagac
    12061 tgcgcatgct cggttatctg cccatcatcc gtttttcgac acagtgtatt aacgagcttt
    12121 tcatgaaatt cgctaaattg caacatagca cagcgttgta ctctctcctt agatatggaa
    12181 gtttttttga atcgcctgat tgtcactgaa acttcctgca agaggtcgca gcctcgaaga
    12241 ttttcagatt tgcgagtgcc actggtgttc tcattcttaa cattggaaga tttctccttc
    12301 cccgcagcca cttttgcgag caaactagcg agggagtagc ctttgttgct ctgatgtttg
    12361 agtgaaggga ggcgaacgac tggcctcata cccccgttgc atatttcctc agtcccccgt
    12421 tcgtttactg ctttaacgtg tatgaggaaa gacctgtact tgcctcccgc catccctttc
    12481 accagctttg gactcgtaag tgtaagcatc cctgcgtggc cggacaagtc caggccggag
    12541 gccaaccaaa cgggaccgca gagaatatct tctttgtggt cttcaaggaa gggacaaagt
    12601 ctgaggcact gactttcctc aacatccatg tgttgcatct cttcattaag gtcaaccaat
    12661 gagggttttc cgttttgctc gacttctata ttcaacgcat tgacttttcc caaccccggt
    12721 gccttgtttt tcagcagagt aacttgaatt tgcggaatat tagtaatgtt actattgagg
    12781 acaaatttga agtcgacatg tccaaccatg catgccttag ggaggaccaa ttcgaatacg
    12841 tgttcgtccc aactgtttga atcggtctgc agtttccacg ttctcgtatg ttgcgcggca
    12901 tccccgtgat gttgttggtg gaggtgttgt gggtgccgtc gctgctgctg ctcttgctgg
    12961 acttctaccc aacagggagg aacagttgcg gagaatcttg gagtcagggt ctcaaatcga
    13021 gtcagctcca ccagtgaagt caagatttcg agcgtgaacg gttgatctac caacaggtca
    13081 acgccagaga tacccggact ggaagggttg ctaaggggct tggagccctc agaagacggt
    13141 tctatcccct tactaaggct cccatcgacc aatatgtcgg cctcgtctat gtcgtcacat
    13201 gtgcctccta tctgcagaaa atgaagttcc ccgcccttgg tgcacgcgca aaggcggtca
    13261 gtgccactac agtagatcac tgagacgaat gtgtcttgct cttctgttcc ttcttttttg
    13321 ggcggttcaa ccttcgccag gatttcgaaa ttgctcaaat caaggatctt aacgtagccg
    13381 ccttgagtcg taattaccag gtgtcccagt gtactgatgt ctgaagtctt ctcccgttcg
    13441 aagccgtttt tggacgtaag ttgcatgtcc tctattggtt cctcacagtc gtcttcccga
    13501 ttgtcgagga tgtcgggggg gaggagaatg agtgatgtga tcgtgtcttg cgggtctttg
    13561 atatgttgaa tctttatggg ctcttcctca agagttacta tgcgtgtagc gtaattcatt
    13621 ttgtagagca ccaaatatcc cccacttacg ttcctttgtt caggctgaat tatgagggga
    13681 gtttgaacat cagcggggga ttcatgttgg ataacggaga tatttgcacc gttgacaacc
    13741 gccaaattgg attcgagttc gcctttccgt ctattacaca gtgcgctatt cagcttgttc
    13801 aggttgttca aagcttcaac ttggtttatt gctgagaggc tctcgactgg gcacgtgcgc
    13861 agtcccacca gcaaatgaat accatctgca caaggcgtta ttgagtctat gcaaagattc
    13921 tcttcttccg cgaatttagg cagtcgcaaa cactgaacca gcgtaccagg cttggggaat
    13981 actgaattcc atttctcgga atcagggctc gtgagatttg ctgcggcgtc tgcgaattgt
    14041 tgaatatatg taacaggtgg gtcttgaagg agcaattgtt cttgtgaatc cagttccatc
    14101 tcgataattt gcgggaccgt gaagccatca tcatgaagac ttttggacat aatattgttc
    14161 atttggctaa atattttccc tgctttttca tcgctctcct tgatagaata cagaagcaat
    14221 acaggcaaag tgcgtctgac caagggagaa ttgagctcgc tattagtaca ggattcatta
    14281 tccgttgaac cctgctcact aatggattca ccctgcgttc gagaaaggcc atcgagtgac
    14341 ctatgagaat ttgaactaat aggtgaagta gctggggatt tgtatgtcag aagtccgccc
    14401 gcaagcagac aggggaaagg aatgttatgc tgctcctggt gcttctcctt cgttttttca
    14461 gatttggagt tagtcaggca gggattcgct cccagttcct caagatcttt tacagtgtct
    14521 tcaagcacat tggccactat ttcccattgg agtttttcag gctgttggag gatagaaagc
    14581 gcggtaatgt cgagggagac ttccatggcc tctttttgtg aggtatgccc agtaacgcta
    14641 tcggacctgc tgtgttcttc ggagtctgaa tcctcgagca ggtcatcact atctccttcg
    14701 agtttcggga tgtcagagca agatgatgaa tcctcgagcc acgcaagcgt aggtcttcgg
    14761 ctatctacac cggaggaggg atcgccactc aagataagtt gctgtacgat ggcagggtca
    14821 tatgcattga tttcgaattt caaatggact ttcataagct ttgagacatc ccagatgcat
    14881 atttttccgc gtttggtagc cgctgcaagg aagtgtggac aggacccgct cccaaagcat
    14941 gatattcgat ctgttccgtc ggtacacgga aattgtgcgg gggacgtagc cagagtaaca
    15001 gagagtggta cattctgcgt gtgttctccc ttgacaaacg ggcaattcgg tgaatgccgt
    15061 tcatgctcgc tccaaggctc atcggtcggc tcccaacata ccaggcatac tgaggaggta
    15121 aaggacatcg ctctgtcatc acctgaagac gcaggttgat ggtaaaatcc agcctgggcc
    15181 atcgggtctg gctgagccca tcgatagccg acatggggcc aggaggtaaa agtttctctc
    15241 cgattagctt cagagtacat aagtgatcga tcgacggacc gcccggggcc aactcccaac
    15301 tctgggcgcg cgctaggcaa gaggtagctc aagcgatcca taactgaact agccactgga
    15361 agggcagcga cgttctgatt tatttttttc agctcgttga ctatagcaga agctatagat
    15421 ttaagcacgt ggtgaggaag gtggaatgtt acggtcgccc atttagctac cttgtgattc
    15481 gctgctgtct cgtggctggt attcttcagg ccatccttaa gctgcgtaat gaagaggtcg
    15541 taaccttcgg tgctgctgat atctactttc tcgaggcagg cagagagcaa ttgctgtgct
    15601 tcggtcacgg ggagttccag ctggacaacg tcgtcctgct tactgaccgg ggtctggagc
    15661 gccgtatcca gcaggagtat cccattcaaa tctttccgac accccacggc atagtcatcg
    15721 acgaaaataa ccttatctac tgctgaaatg tactggcatt taacctgtcc gcctggtttg
    15781 gctgacagcg cggaggcttg gagcgttgcc ccacttgttc catcaatgac cttgatcgtg
    15841 ccgcgggagg taaccgccag aatagcatta agcgctgggt ggtaggagag actatgcagc
    15901 ccgtctgcat cgcaatgcat gcagccatct cgcagtacca gccattcgct aacccccgca
    15961 gccccagctc ctgcggcaga ggaacaacca ggtccggagg cggcggcagc agctgcggcc
    16021 atctttcgac cggcagaaag cactatcacg gatggcagcg gctcagtgac cgtgccgggt
    16081 ggtgccgcac ccccacccgt taccatggtt cgcgagggcc cctggaacag aacttccagc
    16141 ccaccgctac caccgccctt gtcatcgtca tccttgtaat caatgtcatg atctttataa
    16201 tcaccgtcat ggtctttgta gtcacccatg gtggcggcac taggcctggc caactaggcc
    16261 tggccaactt tggacctggg agtggacacc tgtggagaga aaggcaaagt ggatgtcatt
    16321 gtcactcaag tgtatggcca gatcgggcca ggtgaatatc aaatcctcct cgtttttgga
    16381 aactgacaat cttagcgcag aagtaatgcc cgcttttgag agggagtact caccccaaca
    16441 gctggatctc aagcctgcca cacctcacct cgaccatccg cggtgtcaac accgcctact
    16501 ttaattacat catcagcagc acctccgcca gaaacaaccc cgaccgccac ccgctgccgc
    16561 ccgccacggt gctcagccta ccttgcgact gtgactggtt agacgccttt ctcgagaggt
    16621 tttccgatcc ggtcgatgcg gactcgctca ggtccctcgg tggcggagta ccgttcggag
    16681 gccgacgggt ttccgatcca agagtactgg aaagaccgcg aagagtttgt cctcaaccgc
    16741 gagcccaaca gctggccctc gcagacagcg aggcggaaga gagtgaggat ctgacggttc
    16801 actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
    16861 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
    16921 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
    16981 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcaatgac taatacgtag
    17041 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
    17101 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
    17161 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
    17221 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
    17281 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
    17341 actaatgacc ccgtaattga ttactattaa taactagcta gcttaagggc gaatccggac
    17401 cgcaggctga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
    17461 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
    17521 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg
    17581 ttgacttggg tcctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    17641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    17701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    17761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    17821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    17881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    17941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    18001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    18061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    18121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    18181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    18241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    18301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    18361 tgataaatct ggagccggtg agcgtgggtc acgcggtatc attgcagcac tggggccaga
    18421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    18481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aataagcgtc
    18541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    18601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    18661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    18721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    18781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    18841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    18901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    18961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    19021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    19081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    19141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggcttc ctgtggatgc
    19201 gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag
    19261 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
    19321 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
    19381 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact
    19441 atattc
//
