LOCUS       sequence-389080-        4197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoterless insertion vector for split HygR/mScarlet-I landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Banse SA, Patel DS, Lu H, 
            Phillips PC
  TITLE     High-throughput library transgenesis in Caenorhabditis elegans via 
            Transgenic Arrays Resulting in Diversity of Integrated Sequences 
            (TARDIS).
  JOURNAL   Elife. 2023 Jul 4;12:RP84831. doi: 10.7554/eLife.84831.
  PUBMED    37401921
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-4"; volume: "12:RP84831. doi"; pages: " 
            10.7554/eLife.84831"
COMMENT     Sequence Label: pDSP15 (5'Delta HygR + loxP + MCS + 5'Delta  
            mScarlet-I)
FEATURES             Location/Qualifiers
     source          1..4197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(805..829)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1208..1241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1242..1258
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1454..1470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1516..1536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2318..2334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2425..3013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2505..2524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3184..3999)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3907..3926
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4000..4102)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4170..4188
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagtaaaacg
       61 acggccagtt tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt
      121 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcgccat
      181 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
      241 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
      301 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
      361 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
      421 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
      481 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
      541 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
      601 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
      661 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
      721 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
      781 cggtgagttc aggctttttc atggagcctg cttttttgta caaacttgcg gcaccgcctg
      841 acatattacc ttaaaattca aaaattaatt tcagatcagt cataaccaag aaaacaaaat
      901 aaacgttagg acagaaaatt caaattaatt aaaaattatg ctgaataaga aaattacatg
      961 caaagcctta ttttcctcat agaaacagtt gaataacata ttttttaatt gaaaacatag
     1021 caaaattcgt ggtaaaattt gtcaaaacct tggaaaaaat cgaaaaaaga acatttcccg
     1081 atgcattttc ctatttctcg ttgccgacaa cgtataaacg ttgaatactc agtcaacgaa
     1141 tttggctgct ggcttggatt ctcgcacgga agagacgcgt gtgtagattt acgacgaaag
     1201 caaaaatata acttcgtata gcatacatta tacgaagtta tgtaaaacga cggccagtcc
     1261 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggtatctaga
     1321 accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga attcctcgag
     1381 acgtacggtg cgcgcgatgc attcgaagat ctgcccacta gtgagtcgta ttacgtagct
     1441 tggcgtaatc atggtcatag ctgtttcctg atggtttcca agggagaggc tgttatcaag
     1501 gaattcatga cttacccaaa gaagaagcgt aaggtcgaac ttatcaagga gtttatgcgc
     1561 tttaaggttc acatggaagg atctatgaac ggacacgaat tcgaaatcga aggagaagga
     1621 gaaggacgtc catacgaggg aactcaaact gctaagctta aggtaagttt atctaaaaag
     1681 tttttcattc aaaatgtgta aaaattcatt taaaataacc caaaaatcat taatcctcga
     1741 tattttcagg ttactaaagg aggaccactt ccattctctt gggatatcct ttctccacaa
     1801 ttcatgtacg gatctcgcgc tttcatcaag cacccagctg atatcccaga ttactacaag
     1861 caatctttcc cagaaggatt caaatgggag cgtgttatga acttcgaaga tggaggagct
     1921 gttaccgtta cccaagatac ctcccttgag gatggaaccc ttatctacaa ggtattttcc
     1981 tgcatttttc aactgggaaa atgaaagaaa atcgataatt tcaggttaag cttcgtggaa
     2041 ctaatttccc accagatgga ccagttatgc aaaagaagac tatgggatgg gaggcttcta
     2101 ccgagcgcct ttacccagag gatggagtcc ttaagggaga tatcaagatg gctcttcgtc
     2161 ttaaggatgg aggacgttac cttgctgatt tcaaggtaag ttttctagag acctttacta
     2221 actaactagg tctcatagat tttcagacta cttacaaggc taagaagcca gttgtatgat
     2281 cggtccacga tcagctagat tatctagtca gcttgatgtc atagctgttt cctgacatgt
     2341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cagcaaaagg ccaggaaccg
     2401 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgtcgctta ccggatacct
     2581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3181 cagttagaaa aactcatcga gcatcaagtg aaactgcaat ttattcatat caggattatc
     3241 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     3301 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     3361 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     3421 gactgaatcc ggtgagaatg gcaagagttt atgcatttct ttccagactt gttcaacagg
     3481 ccagccatta cgctcgtcat caaaatcact cgcaccaacc aaaccgttat tcattcgtga
     3541 ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa ggacaattac aaacaggaat
     3601 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     3661 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     3721 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3781 gtttagcctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3841 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     3901 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     3961 cggcttcaag caagacgttt cccgttgaat atggctcatt ttagcttcct tagctcctga
     4021 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     4081 ggaacctctt acgtgccgat cacacatttc cccgaaaagt gccacctgac gtctaagaaa
     4141 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
