LOCUS       sequence-389081-        4692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen 
            landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Banse SA, Patel DS, Lu H, 
            Phillips PC
  TITLE     High-throughput library transgenesis in Caenorhabditis elegans via 
            Transgenic Arrays Resulting in Diversity of Integrated Sequences 
            (TARDIS).
  JOURNAL   Elife. 2023 Jul 4;12:RP84831. doi: 10.7554/eLife.84831.
  PUBMED    37401921
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-4"; volume: "12:RP84831. doi"; pages: " 
            10.7554/eLife.84831"
COMMENT     Sequence Label: pDSP16 (5'Delta Cbr-unc-119(+) + loxN + MCS + 
            5'Delta
            ...
FEATURES             Location/Qualifiers
     source          1..4692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1519..1530
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1676..1709)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     1710..1726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1922..1938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1984..2004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2813..2829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2920..3508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3000..3019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3679..4494)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4402..4421
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4495..4597)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4665..4683
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagtaaaacg
       61 acggccagtt tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt
      121 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcgttgc
      181 tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt atcttgccga
      241 ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct cgaaaagcac
      301 ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat agatgttcgc
      361 agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa gggagcaaga
      421 taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga gctttcttcg
      481 caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt ggcatctgga
      541 aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata tcaagagtta
      601 gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg ccgagccggg
      661 tggaatcgat cgttgttgct cggctttcat atcggtttgg ttggaagcgg ctgaaaacgg
      721 aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt agagggtgcc
      781 aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa aatacgataa
      841 tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt tgagtacatg
      901 taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt tttctattta
      961 taacgctaat aatttgaaaa agaaacctat tgcgaaccgc ggggtgaatc ccaaaaacga
     1021 atgcgttttg gtggagtgat tgattcgaat cgaagaagaa aaagaagaag acgtggaata
     1081 gagagctcac tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa atgaatgagg
     1141 gtcttcttct tcttcttctt cgaatgattg acagaaatgg gaaaaagagg aagattgaga
     1201 agggaaaaag gaaggagaaa agaagcagaa gaagacgtca gagaggagag gaacgagcgg
     1261 aaaagcagcg ggcgcaagtc atagaagtag cagagctggg gagaagaaga cactatccaa
     1321 gaaaggaatg acgagagagt atgcaaaggg gtatagggtg cagacagaat aggaacagaa
     1381 taacagatga tgagccaaga agagttgaaa agggcgatga atttgtcatg taacttaatt
     1441 tgggtcaatt tgagcatgat gaattgaaat catcccttgt tgggagttaa taaccggttt
     1501 gttatcagaa accctgtaat agaagggcgc cctaactttg agccaattca tcccggtttc
     1561 tgtcaaatat atcaaaaagt ggtcaactga caaattgttt ttgatattat aataaacatt
     1621 ttatccgtta acaattttcg aatacttttt acaaggactt ggataaattg gctcaataac
     1681 ttcgtatagt ataccttata cgaagttatg taaaacgacg gccagtccgt aatacgactc
     1741 acttaaggcc ttgactagag ggtaccagag ctcacctagg tatctagaac cggtgacgtc
     1801 accatgggaa gcttcgtgga tccagatatc ctgcaggaat tcctcgagac gtacggtgcg
     1861 cgcgatgcat tcgaagatct gcccactagt gagtcgtatt acgtagcttg gcgtaatcat
     1921 ggtcatagct gtttcctgat ggtttccaag ggagaggctg ttatcaagga attcatgact
     1981 tacccaaaga agaagcgtaa ggtcggagaa gaggacaaca tggcttctct tccagctact
     2041 cacgaacttc atattttcgg atctatcaac ggagttgatt tcgatatggt tggacaagga
     2101 actggaaacc caaacgacgg atacgaagag cttaacctta agtctaccaa ggtaagttta
     2161 tctaaaaagt ttttcattca aaatgtgtaa aaattcattt aaaataaccc aaaaatcatt
     2221 aatcctcgat attttcaggg agatcttcaa ttctctccat ggatccttgt tccacacatt
     2281 ggatacggat tccaccaata ccttccatac ccagatggaa tgtctccatt ccaagctgct
     2341 atggttgacg gatctggata ccaagttcac cgtactatgc aattcgagga cggagcttcc
     2401 ctcaccgtca actaccgtta cacttacgaa ggatctcaca tcaaggtatt ttcctgcatt
     2461 tttcaactgg gaaaatgaaa gaaaatcgat aatttcaggg agaagctcaa gttaagggaa
     2521 ccggattccc agccgatgga ccagttatga ccaactctct taccgccgct gattggtgcc
     2581 gctctaagaa gacctaccca aacgataagg taagttttct agagaccttt actaactaac
     2641 taggtctcat agattttcag actatcatct ctactttcaa gtggtcttac actaccggaa
     2701 acggaaagcg ttaccgctct accgctcgca ctacttacac tttcgctaag ccaatggctg
     2761 ccaactacgt atgatcggtc cacgatcagc tagattatct agtcagcttg atgtcatagc
     2821 tgtttcctga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccagca
     2881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgtc
     3061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3661 gtaaacttgg tctgacagtt agaaaaactc atcgagcatc aagtgaaact gcaatttatt
     3721 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     3781 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     3841 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     3901 atcaccatga gtgacgactg aatccggtga gaatggcaag agtttatgca tttctttcca
     3961 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcac caaccaaacc
     4021 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgccgt taaaaggaca
     4081 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     4141 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt
     4201 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     4261 aaattccgtc agccagttta gcctgaccat ctcatctgta acatcattgg caacgctacc
     4321 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     4381 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     4441 gttggaattt aatcgcggct tcaagcaaga cgtttcccgt tgaatatggc tcattttagc
     4501 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     4561 attatggtga aagttggaac ctcttacgtg ccgatcacac atttccccga aaagtgccac
     4621 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4681 ggccctttcg tc
//
