LOCUS       sequence-389085-       10895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to 
            synchronize its transport from the ER (RUSH system); visualized with
            HaloTag. Reporter only (no hook)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW SYT1 reporter
FEATURES             Location/Qualifiers
     source          1..10895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     118..137
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        253..300
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             319..693
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    823..944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(860..879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     914..933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(993..1009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(993..1009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1006..1028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1017..1033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1041..1047,1048..1065,1066..1071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1086..1107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1224..1241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1395..1983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1475..1494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2154..2945,2946..3014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2777..2796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3015..3119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3194..3213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3385..3764
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3766..3964
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3919..3939
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3982..4162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4209..4334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4827..5060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5245..5289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5587..5704
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5749..6195
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      6212..6221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6332..6445
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             7793..8677
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             8684..8707
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8734..9322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8787..8807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9205..9216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9325..9341)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9326..9342)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9847..10027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10053..10070)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10059..10283
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10329..10757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10416..10435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10626..10647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10766..10786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10771..10895,1..205)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
       61 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      121 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
      181 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
      241 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
      301 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
      361 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
      421 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
      481 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
      541 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
      601 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
      661 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
      721 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
      781 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
      841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
      961 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1021 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1081 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1141 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1201 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3121 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     3181 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     3241 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     3301 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     3361 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     3421 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3481 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3541 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3601 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3661 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3721 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3781 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3841 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3901 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     3961 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4021 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4081 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4141 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     4201 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     4261 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     4321 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     4381 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     4441 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     4501 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     4561 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     4621 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     4681 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     4741 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     4801 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     4861 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     4921 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     4981 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5041 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5101 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     5161 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5221 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5281 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5341 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     5401 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     5461 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     5521 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     5581 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     5641 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     5701 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaaag tgcaagtggt
     5761 tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg
     5821 gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga
     5881 aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg
     5941 cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc
     6001 gccggtcccc cgtaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc
     6061 ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc
     6121 tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg
     6181 tgcctgagag cgcaggtacg gctaggccac tgccaccatg gacagtaagg gtagctccca
     6241 gaaaggcagc aggctgcttt tgcttctcgt tgtatctaat cttttgctct gccagggcgt
     6301 agtttccgac attggaacca cgaggggttc catggatgag aagacgacgg gttggcgggg
     6361 tggccatgtg gtagagggct tggcaggtga gcttgaacag ctcagagcta ggttggaaca
     6421 ccatccacaa ggtcaacgag agccgggcag cggctcaagc gggtcctccg gaagttctgg
     6481 ttcatctatg gtgagtgcca gtcatcctga ggccctggcc gcccctgtca ccactgttgc
     6541 gacccttgtc ccacacaatg ccactgagcc agccagtcct ggggaaggga aggaagatgc
     6601 cttttccaag ctgaagcaga agtttatgaa tgagctgcat aaaattccat tgccaccgtg
     6661 ggccttaata gccatagcca tagttgcggt ccttttagtc gtaacctgct gcttttgtgt
     6721 ctgtaagaaa tgtttgttca aaaagaaaaa caagaagaag gggaaggaaa agggaggaaa
     6781 gaacgccatt aacatgaaag acgtgaaaga cttagggaag accatgaagg atcaggccct
     6841 taaggatgac gatgctgaaa ccggactgac tgatggagaa gaaaaggaag agcccaagga
     6901 agaggagaaa ctgggaaagc tccaatattc actggactat gacttccaga ataaccagct
     6961 gttggtggga atcatccagg ctgctgaact gcccgccctg gacatggggg gtacatccga
     7021 tccatacgtc aaagtcttcc tgctgcctga caaaaagaag aaatttgaga ctaaagtcca
     7081 ccggaaaacc ctcaatccag tcttcaatga acaatttact ttcaaggtac cctactcgga
     7141 attaggtggc aaaaccctgg tgatggctgt gtatgacttt gatcgcttct ccaagcacga
     7201 catcatcgga gagttcaaag ttcctatgaa caccgtggat tttggccatg tgaccgagga
     7261 gtggcgcgat ctccagagcg ctgagaaaga agagcaagag aaactgggtg acatctgctt
     7321 ctccctccgc tacgtcccta ctgccggcaa actgactgtt gtcattctgg aagccaagaa
     7381 cctgaagaag atggatgtgg gtggcttatc tgatccctac gtgaagattc acctgatgca
     7441 gaacggtaag aggctgaaga agaaaaagac gacgattaag aagaacacac tcaaccccta
     7501 ctacaacgag tccttcagct ttgaagttcc gttcgagcaa atccagaaag tgcaagtggt
     7561 ggtaactgtt ttggactatg acaagattgg caagaacgac gccatcggca aagtcttcgt
     7621 tggttacaac agcactgggg cggagctgcg acactggtca gacatgctgg ccaacccccg
     7681 gcgacccatc gcacagtggc acactctgca ggtagaggag gaggttgatg ccatgctggc
     7741 tgtcaagaag ggatccggaa gttcggggtc cagcggttct tcaggcagtt ccgaaatcgg
     7801 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     7861 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     7921 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     7981 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     8041 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     8101 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     8161 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     8221 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     8281 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     8341 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     8401 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     8461 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     8521 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     8581 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     8641 gatcgcgcgc tggctgtcta ctctggagat ttccggttct tcagactaca aagacgatga
     8701 cgacaagtaa aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     8761 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8821 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8881 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8941 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9001 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9061 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9121 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9181 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9241 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9301 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     9361 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9421 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9481 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9541 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9601 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     9661 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     9721 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     9781 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     9841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10021 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
    10081 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    10141 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    10201 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    10261 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    10321 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10381 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10441 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10501 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10561 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10621 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10681 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10741 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10801 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10861 ggaaagtccc caggctcccc agcaggcaga agtat
//
