LOCUS       sequence-389092-        9818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to 
            synchronize its transport from the ER (RUSH system); visualized with
            HaloTag. Reporter only (no hook)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 9818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW SYB2 reporter
FEATURES             Location/Qualifiers
     source          1..9818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             48..422
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    552..673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(589..608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     643..662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(722..738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(722..738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(735..757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    746..762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(770..776,777..794,795..800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    815..836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(953..970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1124..1712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1204..1223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1883..2674,2675..2743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2506..2525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2744..2848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2923..2942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3114..3493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3495..3693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3648..3668
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3711..3891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3938..4063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4556..4789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4974..5018
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5316..5433
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5478..5924
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             6310..7191
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     CDS             7213..7326
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             7336..7359
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7386..7974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7439..7459)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7857..7868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7977..7993)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7978..7994)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8499..8679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8705..8722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8711..8935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8981..9409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9068..9087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9278..9299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9418..9438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9423..9752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9603..9738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9665..9684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        join(9800..9818,1..29)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
       61 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
      121 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
      181 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
      241 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
      301 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
      361 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
      421 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
      481 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
      541 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      661 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
      721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     2881 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     2941 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3001 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3061 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     3121 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3181 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3241 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3481 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3541 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3601 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3661 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     3721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     3901 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     3961 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4021 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4081 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4141 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4201 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4261 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4321 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4381 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4441 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4501 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     4561 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     4621 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     4681 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     4741 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     4801 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     4861 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     4921 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     4981 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5041 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5101 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5161 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5221 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5281 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5341 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5401 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5461 ccagtttggt taattaaagt gcaagtggtt ttaggaccag gatgaggcgg ggtgggggtg
     5521 cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg
     5581 cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc
     5641 cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc
     5701 tcagcactga aggcgcgctg acgtcactcg ccggtccccc gtaaactccc cttcccggcc
     5761 accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga
     5821 taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt
     5881 ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcaggtacgg ctaggccacc
     5941 atgtcggcta ccgctgccac cgtcccgcct gccgccccgg ccggcgaggg tggcccccct
     6001 gcacctcctc caaatcttac cagtaacagg agactgcagc agacccaggc ccaggtggat
     6061 gaggtggtgg acatcatgag ggtgaatgtg gacaaggtcc tggagcggga ccagaagcta
     6121 tcggaactgg atgatcgcgc agatgccctc caggcagggg cctcccagtt tgaaacaagt
     6181 gcagccaagc tcaagcgcaa atactggtgg aaaaacctca agatgatgat catcttggga
     6241 gtgatttgcg ccatcatcct catcatcatc atcgtttact tcagcactgg atccggaagt
     6301 tcggggtccg aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
     6361 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     6421 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     6481 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     6541 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     6601 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     6661 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     6721 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     6781 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     6841 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc
     6901 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     6961 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     7021 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     7081 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     7141 gacctgatcg gcagcgagat cgcgcgctgg ctgtctactc tggagatttc cagcggttct
     7201 tcaggcagtt ccatggacga aaaaactaca ggctggcgcg gcggacacgt tgtagaggga
     7261 cttgctggtg agcttgagca actccgggct cggctggagc accatccaca ggggcaacgc
     7321 gagcctggtt cttcagacta caaagacgat gacgacaagt aaaattcgat atcaagctta
     7381 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7441 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7501 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7561 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7621 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7681 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7741 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7801 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7861 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7921 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7981 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8041 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8101 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8161 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8221 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     8281 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     8341 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     8401 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     8461 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     8521 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8581 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8641 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     8701 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8761 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     8821 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     8881 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     8941 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     9001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9301 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9361 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     9421 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9481 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     9541 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9601 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9661 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     9721 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     9781 tcggatctga tcagcacgtg ttgacaatta atcatcgg
//
