LOCUS       sequence-389093-        8978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ER-targeted streptavidin-KDEL hook (RUSH system)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW KDEL Hook
FEATURES             Location/Qualifiers
     source          1..8978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     411..430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(649..753)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(828..847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1019..1398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1400..1598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1553..1573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1616..1796
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1843..1968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2461..2694
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2879..2923
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3221..3338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3383..3829
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4389..4412
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4451..5039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4504..4524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4922..4933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5042..5058)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5043..5059)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5564..5744
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5770..5787)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5776..6000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6046..6474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6133..6152)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6343..6364
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6483..6503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6488..6817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6668..6803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6730..6749
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6865..6912
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6931..7305
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7435..7556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7472..7491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7526..7545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7605..7621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7605..7621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7618..7640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7629..7645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7653..7659,7660..7677,7678..7683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7698..7719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7836..7853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8007..8595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8087..8106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8766..8978,1..579,580..648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
       61 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
      121 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
      181 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
      241 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
      301 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
      361 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
      421 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
      481 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
      541 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
      601 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      661 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
      721 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
      781 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
      841 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      901 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      961 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     1021 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1081 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1141 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1261 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1321 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1381 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1441 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1501 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1561 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     1621 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1681 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1741 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1801 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1861 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1921 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1981 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     2041 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     2101 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     2161 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     2221 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     2281 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     2341 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     2401 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     2461 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2521 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     2581 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     2641 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2701 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2761 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2821 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2881 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2941 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     3001 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     3061 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     3121 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     3181 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     3241 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     3301 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     3361 gagatccagt ttggttaatt aaagtgcaag tggttttagg accaggatga ggcggggtgg
     3421 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
     3481 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
     3541 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
     3601 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgtaaa ctccccttcc
     3661 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
     3721 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
     3781 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagg tacggctagg
     3841 ccaccatgaa actgtctctg gtagctgcaa tgcttcttct cctgagcgcc gcccgcgcaa
     3901 tggaccctag caaagactca aaagctcagg tgtccgctgc cgaggctggc attactggaa
     3961 catggtacaa tcagctcggg agcaccttta ttgtgactgc tggagccgat ggagccctca
     4021 ccggaacata cgaatctgct gtgggaaacg ccgaatcacg gtacgtcctc actggccgat
     4081 acgatagtgc ccctgccacc gacggatctg ggactgccct gggatggact gtcgcttgga
     4141 aaaacaacta ccggaatgct cattctgcca caacatggag tggacagtac gtgggaggcg
     4201 ctgaggctag aatcaataca cagtggctgc tcacatctgg cacaaccgag gcaaatgctt
     4261 ggaaatccac cctggtggga catgacacat tcaccaaagt gaaaccctcc gccgcttcaa
     4321 tcgatgccgc caaaaaagcc ggagtcaaca acggcaatcc tctggatgcc gtccagcagg
     4381 gttcttcaga ctacaaagac gatgacgaca agaaggacga actctaaaat tcgatatcaa
     4441 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4501 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4561 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4621 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4681 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4741 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4801 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4861 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4921 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4981 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5041 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     5101 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5161 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5221 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5281 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5341 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5401 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5461 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5521 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     5761 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5821 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5881 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5941 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6001 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6061 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6121 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6181 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6241 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6301 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6361 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6421 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6481 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6541 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6601 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6661 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6721 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6781 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6841 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     6901 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     6961 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7021 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7081 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7141 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7201 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7261 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7321 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7381 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7561 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8941 ataaaccagc cagccggaag ggccgagcgc agaagtgg
//
