LOCUS       sequence-389094-       19446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Hunkeler M, Jin CY, Fischer ES
  TITLE     Structures of BIRC6-client complexes provide a mechanism of 
            SMAC-mediated release of caspases.
  JOURNAL   Science. 2023 Mar 17;379(6637):1105-1111. doi: 
            10.1126/science.ade5750. Epub 2023 Feb 9.
  PUBMED    36758104
REFERENCE   2  (bases 1 to 19446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade5750"; journalName: "Science"; date: 
            "2023-03-17- 17"; volume: "379"; issue: "6637"; pages: "1105-1111"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)
FEATURES             Location/Qualifiers
     source          1..19446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(17873..17893)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18291..18302)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(18369..18386)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    18375..18582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(18630..18635,18636..18652,18653..18658)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             18847..19380
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc ctcgcgaacc atggtaacgg gtgggggtgc
     3361 ggcaccaccc ggcacggtca ctgagccgct gccatccgtg atagtgcttt ctgccggtcg
     3421 aaagatggcc gcagctgctg ccgccgcctc cggacctggt tgttcctctg ccgcaggagc
     3481 tggggctgcg ggggttagcg aatggctggt actgcgagat ggctgcatgc attgcgatgc
     3541 agacgggctg catagtctct cctaccaccc agcgcttaat gctattctgg cggttacctc
     3601 ccgcggcacg atcaaggtca ttgatggaac aagtggggca acgctccaag cctccgcgct
     3661 gtcagccaaa ccaggcggac aggttaaatg ccagtacatt tcagcagtag ataaggttat
     3721 tttcgtcgat gactatgccg tggggtgtcg gaaagatttg aatgggatac tcctgctgga
     3781 tacggcgctc cagaccccgg tcagtaagca ggacgacgtt gtccagctgg aactccccgt
     3841 gaccgaagca cagcaattgc tctctgcctg cctcgagaaa gtagatatca gcagcaccga
     3901 aggttacgac ctcttcatta cgcagcttaa ggatggcctg aagaatacca gccacgagac
     3961 agcagcgaat cacaaggtag ctaaatgggc gaccgtaaca ttccaccttc ctcaccacgt
     4021 gcttaaatct atagcttctg ctatagtcaa cgagctgaaa aaaataaatc agaacgtcgc
     4081 tgcccttcca gtggctagtt cagttatgga tcgcttgagc tacctcttgc ctagcgcgcg
     4141 cccagagttg ggagttggcc ccgggcggtc cgtcgatcga tcacttatgt actctgaagc
     4201 taatcggaga gaaactttta cctcctggcc ccatgtcggc tatcgatggg ctcagccaga
     4261 cccgatggcc caggctggat tttaccatca acctgcgtct tcaggtgatg acagagcgat
     4321 gtgctttacc tgctcagtat gcctggtatg ttgggagccg accgatgagc cttggagcga
     4381 gcatgaacgg cattcaccga attgcccgtt tgtcaaggga gaacacacgc agaatgtacc
     4441 actctctgtt actctggcta cgtcccccgc acaatttccg tgtaccgacg gaacagatcg
     4501 aatatcatgc tttgggagcg ggtcctgtcc acacttcctt gcagcggcta ccaaacgcgg
     4561 aaaaatatgc atctgggatg tctcaaagct tatgaaagtc catttgaaat tcgaaatcaa
     4621 tgcatatgac cctgccatcg tacagcaact tatcttgagt ggcgatccct cctccggtgt
     4681 agatagccga agacctacgc ttgcgtggct cgaggattca tcatcttgct ctgacatccc
     4741 gaaactcgaa ggagatagtg atgacctgct cgaggattca gactccgaag aacacagcag
     4801 gtccgatagc gttactgggc atacctcaca aaaagaggcc atggaagtct ccctcgacat
     4861 taccgcgctt tctatcctcc aacagcctga aaaactccaa tgggaaatag tggccaatgt
     4921 gcttgaagac actgtaaaag atcttgagga actgggagcg aatccctgcc tgactaactc
     4981 caaatctgaa aaaacgaagg agaagcacca ggagcagcat aacattcctt tcccctgtct
     5041 gcttgcgggc ggacttctga catacaaatc cccagctact tcacctatta gttcaaattc
     5101 tcataggtca ctcgatggcc tttctcgaac gcagggtgaa tccattagtg agcagggttc
     5161 aacggataat gaatcctgta ctaatagcga gctcaattct cccttggtca gacgcacttt
     5221 gcctgtattg cttctgtatt ctatcaagga gagcgatgaa aaagcaggga aaatatttag
     5281 ccaaatgaac aatattatgt ccaaaagtct tcatgatgat ggcttcacgg tcccgcaaat
     5341 tatcgagatg gaactggatt cacaagaaca attgctcctt caagacccac ctgttacata
     5401 tattcaacaa ttcgcagacg ccgcagcaaa tctcacgagc cctgattccg agaaatggaa
     5461 ttcagtattc cccaagcctg gtacgctggt tcagtgtttg cgactgccta aattcgcgga
     5521 agaagagaat ctttgcatag actcaataac gccttgtgca gatggtattc atttgctggt
     5581 gggactgcgc acgtgcccag tcgagagcct ctcagcaata aaccaagttg aagctttgaa
     5641 caacctgaac aagctgaata gcgcactgtg taatagacgg aaaggcgaac tcgaatccaa
     5701 tttggcggtt gtcaacggtg caaatatctc cgttatccaa catgaatccc ccgctgatgt
     5761 tcaaactccc ctcataattc agcctgaaca aaggaacgta agtgggggat atttggtgct
     5821 ctacaaaatg aattacgcta cacgcatagt aactcttgag gaagagccca taaagattca
     5881 acatatcaaa gacccgcaag acacgatcac atcactcatt ctcctccccc ccgacatcct
     5941 cgacaatcgg gaagacgact gtgaggaacc aatagaggac atgcaactta cgtccaaaaa
     6001 cggcttcgaa cgggagaaga cttcagacat cagtacactg ggacacctgg taattacgac
     6061 tcaaggcggc tacgttaaga tccttgattt gagcaatttc gaaatcctgg cgaaggttga
     6121 accgcccaaa aaagaaggaa cagaagagca agacacattc gtctcagtga tctactgtag
     6181 tggcactgac cgcctttgcg cgtgcaccaa gggcggggaa cttcattttc tgcagatagg
     6241 aggcacatgt gacgacatag acgaggccga catattggtc gatgggagcc ttagtaaggg
     6301 gatagaaccg tcttctgagg gctccaagcc ccttagcaac ccttccagtc cgggtatctc
     6361 tggcgttgac ctgttggtag atcaaccgtt cacgctcgaa atcttgactt cactggtgga
     6421 gctgactcga tttgagaccc tgactccaag attctccgca actgttcctc cctgttgggt
     6481 agaagtccag caagagcagc agcagcgacg gcacccacaa cacctccacc aacaacatca
     6541 cggggatgcc gcgcaacata cgagaacgtg gaaactgcag accgattcaa acagttggga
     6601 cgaacacgta ttcgaattgg tcctccctaa ggcatgcatg gttggacatg tcgacttcaa
     6661 atttgtcctc aatagtaaca ttactaatat tccgcaaatt caagttactc tgctgaaaaa
     6721 caaggcaccg gggttgggaa aagtcaatgc gttgaatata gaagtcgagc aaaacggaaa
     6781 accctcattg gttgacctta atgaagagat gcaacacatg gatgttgagg aaagtcagtg
     6841 cctcagactt tgtcccttcc ttgaagacca caaagaagat attctctgcg gtcccgtttg
     6901 gttggcctcc ggcctggact tgtccggcca cgcagggatg cttacactta cgagtccaaa
     6961 gctggtgaaa gggatggcgg gaggcaagta caggtctttc ctcatacacg ttaaagcagt
     7021 aaacgaacgg gggactgagg aaatatgcaa cgggggtatg aggccagtcg ttcgcctccc
     7081 ttcactcaaa catcagagca acaaaggcta ctccctcgct agtttgctcg caaaagtggc
     7141 tgcggggaag gagaaatctt ccaatgttaa gaatgagaac accagtggca ctcgcaaatc
     7201 tgaaaatctt cgaggctgcg acctcttgca ggaagtttca gtgacaatca ggcgattcaa
     7261 aaaaacttcc atatctaagg agagagtaca acgctgtgct atgttgcaat ttagcgaatt
     7321 tcatgaaaag ctcgttaata cactgtgtcg aaaaacggat gatgggcaga taaccgagca
     7381 tgcgcagtct ctggtccttg acacactctg ctggctggcg ggggtacact ccaatggccc
     7441 aggtagctcc aaggaaggta acgaaaatct cctgtcaaaa acccggaaat tcctgagtga
     7501 tattgtgcgc gtctgctttt tcgaagccgg gcgaagtata gcacacaaat gcgctaggtt
     7561 tctcgcgctc tgtatctcaa acggtaagtg cgatccttgc caacccgcgt tcggtccagt
     7621 ccttttgaag gccctcctcg ataacatgtc atttctgccg gctgccacga ctgggggctc
     7681 agtgtactgg tattttgttc ttctgaacta cgtcaaggat gaagatcttg ctggctgcag
     7741 tacggcctgt gcctcacttt tgactgctgt ctcccgccaa ctgcaggatc gcttgacacc
     7801 gatggaagcg cttctccaga cacgatatgg actctactcc tctccgttcg acccagtgct
     7861 gttcgacctt gagatgtctg ggtcttcatg caagaatgta tataacagca gcataggggt
     7921 ccagtctgac gaaatagatt tgtccgacgt actcagtgga aacggtaagg tttcaagttg
     7981 tacggcggct gaaggctcat ttactagcct taccggactt cttgaagtcg agccgctcca
     8041 tttcacatgt gtttcaacat ctgacgggac gcggattgag cgagatgatg ctatgagtag
     8101 cttcggtgtg acaccggcgg tgggagggct ctcctcagga acggtagggg aggcatcaac
     8161 ggctctgagt tccgctgcac aggtgggctc cggctcagct gtcggccccg tacacaatag
     8221 cgtccctagc aaccccgtcg ctgccccggg cttctttata cacccaagcg atgtgatccc
     8281 gcctactcca aaaacgacac ccctgtttat gactccaccc ttgacgccgc caaatgaagc
     8341 tgtttctgtc gtcatcaacg cagagttggc ccagcttttc ccgggttcag ttattgaccc
     8401 gcctgccgtc aatctcgcag cacacaacaa aaactcaaac aaatcccgaa tgaacccttt
     8461 ggggtcagga cttgctcttg ccatctcaca cgctagccac ttccttcagc cccctcccca
     8521 tcaatccatc ataattgaac ggatgcatag tggagctaga cgcttcgtta cgttggattt
     8581 cggaaggcca atcctcctga cagacgttct tatacccacc tgtggcgact tggctagcct
     8641 ttccattgat atatggaccc tgggtgaaga ggttgacggt cgcagactgg ttgtagcaac
     8701 agacattagc actcactccc tgattttgca tgatctcatt ccgccccccg tatgtcgctt
     8761 catgaaaatt acagtcatcg gccggtatgg ttcaacgaat gcaagggcga agataccact
     8821 cggtttttac tatggacaca cgtacattct tccttgggag tccgagctta agttgatgca
     8881 tgacccattg aaaggcgaag gggaatctgc caatcagccc gagattgatc agcaccttgc
     8941 catgatggtg gcattgcaag aagacattca gtgtcgctac aatctggctt gtcaccgctt
     9001 ggaaaccttg ctccagtcca ttgatcttcc accattgaat agcgctaaca acgcgcagta
     9061 cttcctgagg aaacccgaca aagctgtcga ggaagattct agggtcttct ctgcatacca
     9121 ggactgtatt caacttcagc ttcagctcaa ccttgcccac aatgctgttc aacgcctgaa
     9181 ggttgccttg ggggcctcaa gaaagatgct ctctgaaaca agtaatcccg aagacctgat
     9241 acagacaagc tccacagaac aactgcgaac gatcatacgg tacttgctgg acacgcttct
     9301 ctccttgttg cacgcaagca atggacattc cgtgccagca gtcctgcaga gcacattcca
     9361 tgcgcaagct tgtgaggaac tcttcaagca tttgtgtatt tcagggacac caaagattag
     9421 gcttcacaca gggcttttgc tggtgcaatt gtgcggcggg gagaggtggt ggggtcaatt
     9481 tctttccaac gttttgcagg agctttataa ttctgaacaa cttttgatat ttccgcaaga
     9541 ccgggtgttc atgttgctca gctgcattgg acagcgaagc ttgtctaata gcggtgtact
     9601 cgaatccctg ctgaacctct tggataattt gctttccccg ttgcagccac agctccccat
     9661 gcaccgacgg actgaagggg ttcttgacat cccaatgata tcatgggtcg taatgttggt
     9721 cagtcggttg cttgactatg tggcaaccgt agaggatgaa gcagccgcag caaaaaaacc
     9781 tttgaatggc aatcagtggt ctttcataaa caataatctt catacacaat ccttgaaccg
     9841 ctctagcaaa gggtcaagta gcctggatag attgtactca agaaaaatcc ggaaacaact
     9901 cgttcatcat aagcagcagc ttaacctcct caaggcgaaa caaaaggctc tggttgagca
     9961 gatggagaaa gaaaaaattc agtcaaataa gggatctagc tataagttgc tcgttgagca
    10021 ggccaaactt aagcaagcaa cgagtaagca ttttaaagat ctcattaggc tgagacggac
    10081 ggccgagtgg agtagaagta atcttgatac agaagttacc acggccaagg agtctccgga
    10141 aattgaacca ctgcctttca ctttggccca tgaaaggtgc attagcgtgg ttcaaaagct
    10201 tgttcttttc ttgttgtcta tggatttcac atgtcatgcc gacctcttgc tcttcgtttg
    10261 taaggtcttg gcacgaattg ctaatgcgac caggccaaca atacacttgt gcgaaattgt
    10321 aaatgaaccc caactggaaa gattgctgct cttgttggtc gggacggact tcaaccgagg
    10381 cgacatcagc tggggcggcg cctgggccca atactcattg acttgtatgc tccaagatat
    10441 actcgcagga gagttgcttg ctccggtggc cgccgaagcg atggaagaag ggaccgttgg
    10501 tgatgatgtt ggagctacgg cgggggattc cgatgatagt ctgcaacaat catcagttca
    10561 gcttctggag accattgacg aaccccttac ccacgacatc actggtgcac cccccctgtc
    10621 atcacttgag aaagacaaag agatagatct cgagcttctc caggacctta tggaagtaga
    10681 catcgatcca ctcgacatag acctggagaa ggacccattg gcggcaaagg tattcaaacc
    10741 catctcatct acttggtacg attattgggg cgcggactac ggtacatata actataatcc
    10801 ttacataggt ggacttggaa tcccagttgc gaaacctcca gcgaatactg agaagaacgg
    10861 tagtcagact gtatccgtgt ccgtttctca agccctggat gctcgcctgg aagtgggttt
    10921 ggagcagcag gcagagttga tgttgaaaat gatgagcact cttgaagcgg acagtatatt
    10981 gcaggctctg accaatacgt cacctacctt gtctcagagc ccaactggga ccgatgattc
    11041 actccttggt gggcttcaag ctgcgaatca aaccagccag ctgattatac agctctcatc
    11101 tgtacctatg ctgaacgttt gtttcaacaa acttttctcc atgctccagg ttcaccacgt
    11161 gcagcttgag tccttgctcc aactgtggct tacactttcc ctgaatagtt cctctacagg
    11221 caataaggaa aatggtgcag atatctttct gtacaatgcc aatagaatac cggtgattag
    11281 tcttaatcaa gcctctatca ccagcttcct caccgtgctg gcgtggtatc ccaacacact
    11341 tttgcgcacc tggtgcttgg ttttgcatag tctgaccctg atgacaaata tgcaattgaa
    11401 ttcaggtagt tccagcgcta taggaactca ggaatctacg gcacacctgt tggtgtcaga
    11461 tccaaacctg atccacgtac tggttaaatt cctctctggt acttccccgc atggtacgaa
    11521 ccaacacagc ccacaagttg gacctacagc cacccaggcg atgcaggagt ttttgaccag
    11581 actccaggta cacctttctt caacctgccc tcagatattc tcagaattcc tcctcaagct
    11641 tattcatatc ctcagtaccg aaagaggggc gttccagacg gggcaaggtc ctttggatgc
    11701 tcaagtcaag ctgttggagt tcacccttga acaaaacttc gaggttgtaa gtgtctctac
    11761 tatatctgct gtcatcgaaa gcgttacgtt ccttgtacac cattatatca catgctcaga
    11821 taaggtcatg tcaagaagtg gctcagacag tagtgtgggc gctcgggctt gttttggtgg
    11881 tctcttcgct aatctgatcc gacctggaga cgcaaaagca gtgtgcggag agatgaccag
    11941 agatcagttg atgttcgatc ttctgaagct cgtcaacatc cttgtgcaac tgcctttgag
    12001 tggcaatagg gaatacagtg ctagagtaag cgtcacaact aatacgacgg attccgtttc
    12061 cgacgaagaa aaagtgtccg ggggtaaaga tggaaatggt tccagcacca gcgtacaagg
    12121 atccccagcc tacgtggcgg atctcgtgct cgccaatcaa cagataatga gtcaaattct
    12181 gtccgcactc ggcttgtgca attcctctgc aatggccatg attataggag cttctgggtt
    12241 gcatttgact aagcatgaaa actttcatgg cggtttggat gcgatctctg tgggagatgg
    12301 acttttcacg attctgacta ctttgagcaa gaaagcgtct acggttcaca tgatgctgca
    12361 gccgatcttg acatatatgg catgtggata catgggccgg caggggtcac ttgccacatg
    12421 ccagttgtct gagcctctgc tgtggttcat actccgagtc ctcgatactt ctgacgcgct
    12481 taaagccttc catgacatgg gaggagtgca gctcatctgc aataacatgg ttacaagcac
    12541 tagagcgatt gtcaacacag caagatctat ggtctccacc attatgaagt tccttgactc
    12601 tgggccgaac aaggcagtcg attctacctt gaagacgagg atccttgcct ctgagccaga
    12661 taatgctgaa ggaatacaca attttgcacc tttgggtaca attacgagct cttctccaac
    12721 ggcacagccg gcggaagtcc tgcttcaagc cactcctcct caccggaggg ccagatccgc
    12781 agcttggtca tatatattct tgccagagga agcatggtgc gatctcacta ttcatctccc
    12841 tgccgcggtg ctccttaagg aaatccatat tcagcctcac ctggcttctt tggctacgtg
    12901 tccatcctct gtatcagtcg aggttagtgc ggatggagta aatatgctcc cgctcagtac
    12961 tccggtcgta acttcaggac ttacctacat aaagatccaa cttgtaaaag ctgaggtagc
    13021 ttccgcggtc tgcttgcgct tgcaccgccc ccgcgacgct tccacgctcg ggctttccca
    13081 gattaaactc ctggggctta cagcgtttgg gaccacatct tctgctacgg taaataatcc
    13141 gtttttgcct tccgaagatc aagttagcaa aacatctatc ggatggctcc ggctcctcca
    13201 tcactgtctc acgcatatct cagatctgga aggaatgatg gcttcagcgg ctgcccccac
    13261 ggccaatctt ctccagactt gcgccgcact tctcatgtcc ccctattgcg gaatgcactc
    13321 acccaatatt gaggtcgtct tggttaaaat cggcctccag tcaactcgga taggtctcaa
    13381 gcttatagac atcttgttgc ggaattgtgc agctagtggt tctgatccta cagatctgaa
    13441 cagtccactg ttgtttggtc ggttgaatgg cctctctagc gatagtacaa tagacatttt
    13501 gtatcaactt ggaactaccc aagatccggg aaccaaagat cggattcagg cattgctcaa
    13561 atgggtaagc gatagcgcgc gcgtagcagc tatgaaaaga tctggccgaa tgaattacat
    13621 gtgcccgaat tccagcactg tagaatacgg gcttctgatg ccgtcaccga gtcacctgca
    13681 ttgcgttgcg gcaattctgt ggcactccta tgagttgctc gttgaatacg acttgccagc
    13741 actgctggat caggagctct ttgagcttct gtttaattgg tccatgtctc tgccgtgtaa
    13801 tatggttctc aaaaaggctg ttgacagctt gctttgctct atgtgtcacg tacatcctaa
    13861 ctacttctct ctgttgatgg gttggatggg cataaccccc ccgccggttc aatgccatca
    13921 ccgcttgtcc atgacagatg acagcaaaaa gcaagacttg tcttcatctc tgacagatga
    13981 ttctaaaaat gcccaagcac ccttggcgct gactgaaagc cacttggcta ccctcgcaag
    14041 ttcttcacag tctcctgaag ctattaagca gctcctggac agcgggctgc cgagcctcct
    14101 tgttcggagc ttggcttcat tctgtttttc ccacataagt agtagtgaat ccattgcaca
    14161 gagcattgac atttcacagg ataaactccg gcggcatcat gttccccagc aatgtaacaa
    14221 aatgccgatt acggcggatc tcgtcgcccc gatattgcgg ttccttaccg aggtcggtaa
    14281 cagccacatc atgaaagact ggctgggtgg ttccgaagtc aatcctctct ggactgcact
    14341 gctgttcttg ttgtgtcatt ctgggagtac cagcggctca cacaatctcg gggcccagca
    14401 gacgagcgct aggtctgcca gcttgagttc tgccgctact acgggtctta caacacaaca
    14461 aagaaccgct atagaaaacg cgacagtggc gttttttctc cagtgcataa gttgccaccc
    14521 aaataatcaa aagcttatgg ctcaagtgct ttgcgagctc tttcagacca gtccacagag
    14581 gggcaatctc ccgacgtctg gcaacataag cggttttatc agacgactct tccttcaact
    14641 catgctggag gacgaaaagg tcactatgtt cctgcagagc ccgtgcccgc tctataaggg
    14701 aaggataaat gccacgagcc atgtcattca gcacccgatg tacggggccg ggcacaaatt
    14761 tagaacactc cacctgccag tatctacgac gctttcagat gtccttgata gggtctccga
    14821 tactccgagc atcaccgcca agcttatctc cgagcagaaa gatgacaaag aaaaaaaaaa
    14881 ccacgaggaa aaagagaaag tcaaagccga aaatggcttt caggataact actccgtggt
    14941 agtcgcctcc gggctcaaga gtcaaagcaa gagggcggtc agcgcgaccc ctcctcgacc
    15001 accatcacgc cgcgggagga cgataccgga caagatcgga agcacgagcg gcgccgaggc
    15061 agcgaataaa atcatcacgg taccagtgtt tcatctgttc cataagcttc tcgcaggtca
    15121 gccactgcca gcggaaatga ctctggctca actgctcacc ctgctttatg accgcaagtt
    15181 gcctcagggg tacagatcta tagatcttac ggttaagctg ggcagtcggg tgattacaga
    15241 cccttcactg tccaaaactg attcctataa gcgcttgcac cctgaaaagg atcacggtga
    15301 cctccttgct tcctgcccag aggacgaggc cttgacaccc ggcgacgaat gcatggacgg
    15361 aattttggat gaaagcctgc tggaaacctg tccgatacaa tccccgctcc aggtattcgc
    15421 cgggatgggt ggtcttgctc tcatcgccga gaggttgccc atgctttatc ctgaagtgat
    15481 ccagcaggta tcagctccgg ttgtaacctc aaccacccaa gagaaaccta aagattcaga
    15541 tcaatttgaa tgggtgacaa ttgagcagag cggcgaattg gtgtatgagg cccccgaaac
    15601 ggttgctgcc gagccgcccc caataaaatc cgccgtccag acaatgtctc caataccagc
    15661 gcactctttg gcggcatttg ggctttttct taggctccct ggttacgctg aagtcctgct
    15721 gaaagaacgc aagcatgcgc aatgccttct gcggctcgtt ttgggggtga cggacgatgg
    15781 agaaggttcc catatccttc agagccccag cgcgaacgta ttgcccacac ttccttttca
    15841 tgtgctccga tccctctttt caactacccc cctcacaact gatgacggcg tccttctgcg
    15901 acgaatggcc cttgagatcg gggccttgca cctcattctc gtgtgcctca gtgcgttgag
    15961 ccaccacagt ccccgggtcc caaacagcag cgtgaaccag acggaaccac aggtgagcag
    16021 ctcccacaac cccacgagta ccgaggagca gcagctctac tgggcaaaag ggaccggatt
    16081 tgggactggc tcaaccgcct ccggatggga tgtggaacaa gctcttacga agcaaaggtt
    16141 ggaggaggaa catgtaactt gtctgctcca ggtgcttgcg agctatataa accctgtgtc
    16201 ttcagcggtg aatggcgagg cacaatccag tcacgaaacg cgcggtcaga attccaatgc
    16261 tctccccagt gtgctcctgg agcttcttag ccagagttgt ttgatacctg ccatgagttc
    16321 ataccttcga aacgactctg tgctggacat ggcgagacat gttcccttgt atagagctct
    16381 tctggaactg ctgagagcta tagccagttg cgctgccatg gtgcctctcc tgctccccct
    16441 gagcaccgag aatggcgagg aggaggagga gcagagcgag tgccagacca gtgttggcac
    16501 tctccttgcc aaaatgaaaa cttgtgtcga tacttatacc aaccgcctgc ggtctaagag
    16561 agagaatgtt aaaaccggag ttaagccaga cgcatctgac caggaacccg aagggcttac
    16621 tttgctcgta ccagatatac agaaaacagc tgaaattgtt tacgctgcaa caacatcact
    16681 ccgacaggcc aaccaggaga agaaacttgg ggagtattca aagaaagcgg ccatgaagcc
    16741 taagccgctg tccgtcctga aatcccttga ggaaaagtat gtcgcagtga tgaaaaagct
    16801 ccaattcgac acttttgaaa tggtgtctga agacgaggac ggcaagctgg gattcaaagt
    16861 gaactatcat tatatgagcc aggtcaaaaa cgctaacgat gcaaatagtg cagctagggc
    16921 tcggcgcctt gctcaagaag cggttaccct cagtactagt ctccccttgt catccagtag
    16981 ttcagtcttc gtgagatgtg atgaagagag attggatatt atgaaagttc tgatcaccgg
    17041 ccccgcagac actccttatg caaacggatg cttcgagttt gacgtttact tcccacagga
    17101 ttacccttcc agcccgcccc ttgtcaattt ggaaactacc gggggtcact ccgtacgctt
    17161 caaccccaac ctgtataacg acggcaaagt ttgcctctca attctgaata catggcatgg
    17221 aagacccgaa gaaaaatgga acccgcaaac atcatcattt ctccaggtac tcgtgtccgt
    17281 tcaatcattg atcctcgtgg cggaacctta ctttaacgag cctggttacg agaggtctcg
    17341 aggcacacca tctggtactc aatccagcag ggagtacgat ggcaatatcc gccaagctac
    17401 agtgaagtgg gctatgctcg aacagattag gaatccctct ccttgcttca aggaggtgat
    17461 acataaacat ttctatttga aaagagtaga aattatggcg cagtgtgaag agtggatagc
    17521 agacatccaa caatattcct cagacaaacg ggtcggacgg actatgtctc atcacgcagc
    17581 ggctctcaaa cgacatacgg cccaactccg cgaggaactc ttgaaattgc cgtgtcctga
    17641 gggtttggat cctgacacgg acgacgcccc agaagtatgt agagccacta caggtgcgga
    17701 agagaccctt atgcacgatc aggtaaaacc ctcaagttca aaggaacttc cgtccgactt
    17761 tcagctttga gcggccgctg atgataatgg cctgtggccg taatggcctg tggccgtaaa
    17821 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
    17881 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    17941 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    18001 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    18061 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    18121 ttgccacggc ggaactcagt gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    18181 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
    18241 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    18301 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcggcttc
    18361 gagatctgcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    18421 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    18481 tgcatcaaat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    18541 caagggggag gattgggcag acaatagcag gcatgctggg gactcgagtt aagggcgaat
    18601 cgctgggacg cccgaacggt ggctactcgt tgacataagc ctgttcggtt cgtaaactgt
    18661 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    18721 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
    18781 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
    18841 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
    18901 ttaggtggct caagtatggg catcattcgt acatgtaggc tcggccctga ccaagtcaaa
    18961 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gtgatgtagc cacctactcc
    19021 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac atttattgcg
    19081 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
    19141 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
    19201 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
    19261 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
    19321 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
    19381 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
    19441 cgccgc
//
