LOCUS       sequence-389097-       10052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 
            a.a.) to synchronize its transport from the ER (RUSH system); 
            visualized with HaloTag. Reporter only (no hook)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW SYT1deltaC2AB reporter
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2000..2019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2191..2570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2572..2770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2725..2745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2788..2968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3015..3140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3633..3866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4051..4095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4393..4510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4555..5001
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      5018..5027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5138..5251
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             5294..6178
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             6647..6670
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6697..7285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6750..6770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7168..7179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7288..7304)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7289..7305)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7810..7990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8016..8033)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8022..8246
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8292..8720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8379..8398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8589..8610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8729..8749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8734..9063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8914..9049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8976..8995
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9111..9158
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9177..9551
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9681..9802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9718..9737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9772..9791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9851..9867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9851..9867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9864..9886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9875..9891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9899..9905,9906..9923,9924..9929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9944..9965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     1981 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     2041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     2101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     2161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     2221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2401 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2461 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2521 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2581 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2641 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2701 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2761 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2941 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     3001 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     3061 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     3121 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     3181 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     3241 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     3301 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3361 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3421 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3481 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3541 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3601 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3661 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3721 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3781 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3841 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3901 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3961 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     4021 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     4081 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     4141 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     4201 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     4261 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     4321 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     4381 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4441 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     4501 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaaagtgca
     4561 agtggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac
     4621 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg
     4681 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg
     4741 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg
     4801 tcactcgccg gtcccccgta aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc
     4861 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat
     4921 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc
     4981 gtgtcgtgcc tgagagcgca ggtacggcta ggccactgcc accatggaca gtaagggtag
     5041 ctcccagaaa ggcagcaggc tgcttttgct tctcgttgta tctaatcttt tgctctgcca
     5101 gggcgtagtt tccgacattg gaaccacgag gggttccatg gatgagaaga cgacgggttg
     5161 gcggggtggc catgtggtag agggcttggc aggtgagctt gaacagctca gagctaggtt
     5221 ggaacaccat ccacaaggtc aacgagagcc gggatccgga agttcggggt ccagcggttc
     5281 ttcaggcagt tccgaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     5341 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     5401 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     5461 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     5521 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     5581 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     5641 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     5701 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     5761 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     5821 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     5881 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     5941 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     6001 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     6061 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     6121 cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga tttccggcag
     6181 cggctcaagc gggtcctccg gaagttctgg ttcatctatg gtgagtgcca gtcatcctga
     6241 ggccctggcc gcccctgtca ccactgttgc gacccttgtc ccacacaatg ccactgagcc
     6301 agccagtcct ggggaaggga aggaagatgc cttttccaag ctgaagcaga agtttatgaa
     6361 tgagctgcat aaaattccat tgccaccgtg ggccttaata gccatagcca tagttgcggt
     6421 ccttttagtc gtaacctgct gcttttgtgt ctgtaagaaa tgtttgttca aaaagaaaaa
     6481 caagaagaag gggaaggaaa agggaggaaa gaacgccatt aacatgaaag acgtgaaaga
     6541 cttagggaag accatgaagg atcaggccct taaggatgac gatgctgaaa ccggactgac
     6601 tgatggagaa gaaaaggaag agcccaagga agaggagggt tcttcagact acaaagacga
     6661 tgacgacaag taaaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     6721 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6781 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6841 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6901 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6961 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7021 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7081 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7141 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7201 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7261 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     7321 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7381 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7441 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7501 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7561 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7621 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7681 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7741 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7801 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7861 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7921 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7981 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8221 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8281 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8641 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8701 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8761 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8821 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9001 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9061 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9121 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9181 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9241 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     9301 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     9361 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     9421 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     9481 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     9541 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     9601 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     9661 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     9721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9781 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10021 attaatgaat cggccaacgc gcggggagag gc
//
