LOCUS       sequence-389101-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for viral expression of full-length wild type synaptophysin 
            (SYP). Visualized by a C-terminal GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW SYP-GFP
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(433..1224,1225..1293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1056..1075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1294..1398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1473..1492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1664..2043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2045..2243
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2198..2218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2261..2441
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2488..2613
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3106..3339
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3524..3568
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3866..3983
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4028..4474
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      4538..4547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5510..5530
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(5609..5630)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6217..6238
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6331..6919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6384..6404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6802..6813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6922..6938)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6923..6939)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7444..7624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7650..7667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7656..7880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7926..8354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8013..8032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8223..8244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8363..8383)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8368..8697
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8548..8683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8610..8629
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8745..8792
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8811..9185
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9315..9436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9352..9371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9406..9425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9485..9501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9485..9501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9498..9520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9509..9525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9533..9539,9540..9557,9558..9563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9578..9599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9716..9733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9887..10213,1..262))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9967..9986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
       61 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
      961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     1441 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1501 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1561 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1621 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1681 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1741 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1801 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1861 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1921 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1981 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2041 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2101 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2161 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2221 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     2281 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2341 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2401 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2461 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2521 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2581 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2641 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2701 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2761 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     2821 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     2881 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2941 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     3001 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3061 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3121 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3181 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3241 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     3301 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3361 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3421 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3481 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3541 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3601 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3661 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     3721 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     3781 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     3841 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3901 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3961 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     4021 taattaaagt gcaagtggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
     4081 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
     4141 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
     4201 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
     4261 aggcgcgctg acgtcactcg ccggtccccc gtaaactccc cttcccggcc accttggtcg
     4321 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
     4381 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
     4441 cagcggagga gtcgtgtcgt gcctgagagc gcaggtacgg ctagcggatc ggttcaaagt
     4501 ttttttcttc catttcaggt gtcgtgagga attgcccgcc accatggacg tggtgaatca
     4561 gctggtggct gggggtcagt tccgggtggt caaggagccc cttggcttcg tgaaggtgct
     4621 gcagtgggtc tttgccatct tcgcctttgc tacgtgtggc agctacaccg gggagcttcg
     4681 gctgagcgtg gagtgtgcca acaagacgga gagtgccctc aacatcgaag ttgaattcga
     4741 gtaccccttc aggctgcacc aagtgtactt tgatgcaccc tcctgcgtca aagggggcac
     4801 taccaagatc ttcctggttg gggactactc ctcgtcggct gaattctttg tcaccgtggc
     4861 tgtgtttgcc ttcctctact ccatgggggc cctggccacc tacatcttcc tgcagaacaa
     4921 gtaccgagag aacaacaaag ggcctatgat ggactttctg gctacagccg tgttcgcttt
     4981 catgtggcta gttagttcat cagcctgggc caaaggcctg tccgatgtga agatggccac
     5041 ggacccagag aacattatca aggagatgcc catgtgccgc cagacaggga acacatgcaa
     5101 ggaactgagg gaccctgtga cttcaggact caacacctca gtggtgtttg gcttcctgaa
     5161 cctggtgctc tgggttggca acttatggtt cgtgttcaag gagacaggct gggcagcccc
     5221 attcatgcgc gcacctccag gcgccccgga aaagcaacca gcacctggcg atgcctacgg
     5281 cgatgcgggc tacgggcagg gccccggagg ctatgggccc caggactcct acgggcctca
     5341 gggtggttat caacccgatt acgggcagcc agccagcggt ggcggtggct acgggcctca
     5401 gggcgactat gggcagcaag gctatggcca acagggtgcg cccacctcct tctccaatca
     5461 gatgaaagga tccggaagtt cggggtccag cggttcttca ggcagttccg aggatctgta
     5521 ctttcagagc gataacgatg gatccgggtc aggatcatct ggcagttcgg atagcactga
     5581 gagcctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5641 caagttcagc gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa
     5701 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5761 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5821 gtccgccatg cccgaaggct acgtccagga gcgcaccatc accttcaagg acgacggcac
     5881 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5941 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt
     6001 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     6061 caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6121 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6181 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6241 cgccgccggg atcactctcg gcggtacctc aggcagtacg gaaacctctc aggtggcgcc
     6301 ggcatgaaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     6361 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6421 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6481 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6541 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6601 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6661 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6721 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6781 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6841 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6901 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     6961 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7021 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7081 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7141 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7201 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7261 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     7321 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     7381 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     7441 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7501 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7561 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7621 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7681 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7741 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7801 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7861 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7921 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7981 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8041 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8101 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8161 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8221 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8281 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8341 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8521 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8581 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     8641 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8701 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     8761 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     8821 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     8881 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     8941 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9001 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9061 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9121 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9181 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9241 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9301 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9361 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9421 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10201 ccaacccggt aag
//
