LOCUS       sequence-389102-        9776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for viral expression of the light chain of tetanus toxin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW TeTx-LC
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     583..602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(821..925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1000..1019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1191..1570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1572..1770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1725..1745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1788..1968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2015..2140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2633..2866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3051..3095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3393..3510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3555..4001
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    5421..6009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5474..5494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5892..5903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6012..6028)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6013..6029)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6534..6714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6740..6757)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6746..6970
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7016..7444
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7103..7122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7313..7334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7453..7473)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7458..7787
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7638..7773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7700..7719
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7835..7882
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7901..8275
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8405..8526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8442..8461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8496..8515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8575..8591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8575..8591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8588..8610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8599..8615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8623..8629,8630..8647,8648..8653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8668..8689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8806..8823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8977..9565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9057..9076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9736..9776,1..751,752..820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
       61 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
      181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
      241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
      301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
      481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
      961 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     1021 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     1081 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     1141 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     1201 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1261 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1321 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1381 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1441 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1501 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1561 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     1621 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     1681 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     1741 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     1801 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1861 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1921 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1981 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2041 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2101 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2161 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2221 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2281 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2341 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2401 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2461 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2521 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2581 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2641 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2701 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2761 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2821 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2881 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2941 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3001 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3061 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3121 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3181 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3241 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3301 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     3361 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3421 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3481 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3541 gtttggttaa ttaaagtgca agtggtttta ggaccaggat gaggcggggt gggggtgcct
     3601 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
     3661 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
     3721 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
     3781 gcactgaagg cgcgctgacg tcactcgccg gtcccccgta aactcccctt cccggccacc
     3841 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
     3901 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
     3961 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca ggtacggcta gcggatcgcc
     4021 accatgccga tcaccatcaa caacttccgt tactccgacc cggttaacaa cgacaccatc
     4081 atcatgatgg aaccgccgta ctgcaaaggt ctagacatct actacaaagc cttcaaaatc
     4141 accgaccgta tctggatcgt tccggaacgt tacgaatttg gtaccaaacc ggaagacttc
     4201 aacccgccgt cctccctgat cgaaggtgct tccgaatact acgacccgaa ctacctgcgt
     4261 accgactccg acaaagaccg tttcctgcag accatggtta aactgttcaa ccgtatcaaa
     4321 aacaacgttg ctggtgaagc tctgctggac aaaatcatca acgctatccc gtacctgggt
     4381 aactcctact ccctgctgga caaattcgac accaactcca actccgtttc cttcaacctg
     4441 ctggaacagg atccgtccgg tgctaccacc aaatccgcta tgctgaccaa cctgatcatc
     4501 ttcggtccgg gtccggttct gaacaaaaac gaagttcgtg gtatcgttct gcgtgttgac
     4561 aacaaaaact acttcccgtg ccgtgacggt ttcggttcca tcatgcagat ggctttctgc
     4621 ccggaatacg taccgacctt cgacaacgtt atcgaaaaca tcacctccct gaccatcggt
     4681 aaatccaaat acttccagga cccggctctg ctgctgatgc acgaactgat ccacgttctg
     4741 cacggtctgt acggcatgca ggtttcctcc cacgaaatca tcccgtccaa acaggaaatc
     4801 tacatgcagc acacctaccc gatctccgct gaagagctct tcaccttcgg tggtcaggac
     4861 gctaacctga tctccatcga tatcaaaaac gacctgtacg aaaaaaccct gaacgactac
     4921 aaagctatcg ctaacaaact gtcccaggtt acctcctgca acgacccgaa catcgacatc
     4981 gactcctaca aacagatcta tcagcagaag tatcagtttg ataaggattc caacggccag
     5041 tatatagtga acgaggataa gtttcagata ctgtataact ccataatgta tggctttact
     5101 gagatagagc tgggcaagaa gtttaacata aagactcgct taagctattt ttccatgaac
     5161 cacgatcctg tgaagatacc taacctgctg gatgatacta tatataacga tactgagggc
     5221 tttaacatag agtccaagga tctgaagtcc gagtataagg gccagaacat gagggtgaac
     5281 actaacgcct ttaggaacgt cgacggttcc ggtctggttt ccaaactgat cggtctgtgc
     5341 aaaaaaatca tcccgccgac caacatccgt gaaaacctgt acaaccgtac cgcttaaaat
     5401 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5461 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5521 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5581 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5641 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5701 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5761 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5821 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5881 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5941 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6001 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     6061 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6121 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6181 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6241 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6301 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6361 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6421 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6481 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6721 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6781 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6841 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6901 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6961 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7141 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7201 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7261 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7321 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7441 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7501 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7561 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7621 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7681 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7741 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7801 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7861 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7921 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7981 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8041 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8101 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8161 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8221 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8281 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8341 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8401 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8521 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8581 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8641 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8701 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8761 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8821 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8881 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatct
//
