LOCUS       sequence-389107-       11584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of ZFPM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-ZFPM2
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    128..146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    170..188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        305..343
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     340..364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     443..462
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        443..461
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    493..517
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3584..3608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3634..3675
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3685..3702
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3752..4340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3805..3825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4223..4234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4343..4359)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4344..4360)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4865..5045
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5071..5088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5077..5301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5347..5775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5644..5665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5784..5804)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5789..6118
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5969..6104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6031..6050
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6166..6213
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6232..6606
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6736..6857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6773..6792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6827..6846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6906..6922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6906..6922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6919..6941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6930..6946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6954..6960,6961..6978,6979..6984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6999..7020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7137..7154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7308..7896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7388..7407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8067..8858,8859..8927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8690..8709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8928..9032)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9107..9126)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9298..9677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9679..9877
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9895..10075
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10122..10247
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10740..10973
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11158..11202
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(11500..11584,1..33)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
       61 ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
      301 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      421 cagcctccgc ggccccgaat tgtaatacga ctcactatag ggagacccaa gctggctagg
      481 taagcttgat caacaagttt gtacaaaaaa gcaggcttca ccatggactt gaataataat
      541 tctttgaaga caaaggctca ggtcccaatg gtgctgactg ctggtcccaa gtggttgctg
      601 gatgtgactt ggcaaggagt ggaagacaac aaaaacaact gcattgtgta cagcaaaggg
      661 ggtcagcttt ggtgtacaac tacgaaggcc atctctgagg gtgaagagct aattgccttt
      721 gtggtggatt ttgactcaag gctacaagct gccagtcaga tgactctcac agaagggatg
      781 taccctgcac gcctgctgga ctcaattcag ctgcttcctc agcaagctgc catggcttct
      841 attttgccca cagctattgt caataaggat atattccctt gcaagtcctg tggcatctgg
      901 tatcggagtg agcggaatct gcaggcccat ttgatgtact actgcagtgg gaggcaaaga
      961 gaagctgctc cggtgtcaga ggaaaatgaa gacagtgccc atcagatttc cagcctgtgc
     1021 cccttcccac agtgcaccaa gagcttttca aatgctcgag ctctagaaat gcacctgaat
     1081 tcacacagtg gagtgaaaat ggaagaattc ctgccccctg gtgctagtct aaaatgcacc
     1141 gtctgtagct acactgctga ttccgtgatc aactttcacc aacacctgtt ctcccatctc
     1201 actcaagctg ccttccgatg taatcactgc catttcggct tccagactca gagggagtta
     1261 ttgcagcacc aggagctcca tgtccctagc ggcaaacttc ccagagaaag tgacatggaa
     1321 cactctccaa gtgcaactga agacagctta cagccagcca cagacttatt gaccagaagc
     1381 gaacttcccc agagccaaaa ggccatgcag actaaagatg cgagctctga cacagagctg
     1441 gacaagtgtg agaaaaagac tcagctcttt ctcacgaacc agagaccaga gatacagcct
     1501 acaacaaata aacaaagctt ttcttacaca aaaataaagt ctgagccctc tagcccaaga
     1561 cttgcctcat ctccagttca gcctaatatt gggccttctt tccctgtggg ccctttccta
     1621 tctcagtttt ctttccccca agatatcacc atggtccctc aagcttcaga gatcttagct
     1681 aagatgtctg aactggtgca tcggcgactg aggcatggca gtagtagcta ccctcccgtc
     1741 atttacagcc ctttgatgcc caagggggct acttgttttg agtgtaacat aacattcaat
     1801 aatttggata attatctagt gcacaaaaag cattattgca gcagccgatg gcagcagatg
     1861 gctaagtccc cagagttccc tagtgtgtca gaaaagatgc ctgaagcttt gagtcccaac
     1921 actggccaaa cctccataaa ccttctcaac ccagctgctc attctgctga tcctgagaat
     1981 ccacttcttc aaacatcttg catcaattct tccactgtct tagatttaat tgggccaaat
     2041 gggaagggcc atgacaagga cttttccact caaactaaga agctctccac ctccagtaac
     2101 aatgatgaca aaattaatgg aaaacctgtt gatgtgaaaa atcccagtgt ccccttagtg
     2161 gatggggaaa gtgacccaaa taagactacc tgtgaagctt gcaacattac cttcagccgg
     2221 cacgaaacat acatggtcca caaacagtat tactgtgcta cacgccacga ccctccactg
     2281 aagaggtctg cttccaacaa agtgcctgcc atgcagagaa ccatgcgcac acgcaagcgc
     2341 agaaagatgt atgagatgtg cctacctgag caggaacaaa ggcctccact ggttcagcag
     2401 agatttcttg acgtagccaa cctcaataat ccttgtacct ccactcaaga acccacagaa
     2461 gggctaggag agtgctacca cccaagatgt gatatctttc caggaattgt ctctaaacac
     2521 ttggaaactt ctctgacgat caacaagtgt gttccagttt ccaaatgtga tactactcat
     2581 tccagtgttt cctgcctaga gatggacgtg cccatagatc tcagcaaaaa gtgtttatct
     2641 cagtctgagc ggacgaccac gtctcccaaa aggctgctgg actatcacga gtgcactgtg
     2701 tgcaagatca gtttcaataa ggtagaaaac tatctggccc acaagcagaa tttctgcccg
     2761 gttactgcac atcagcgtaa tgacctgggt caactggacg gcaaagtgtt tccgaatcca
     2821 gaaagcgaac gaaacagccc tgatgtcagc tacgaaagaa gcataataaa atgtgagaaa
     2881 aatgggaatt tgaagcagcc ttcccccaat ggaaacttat tttcatccca cctagcaacc
     2941 ctgcaaggct tgaaggtctt tagtgaagct gctcagctca ttgctacaaa agaagaaaac
     3001 agacatttgt ttcttccaca atgcctttac cctggagcaa taaagaaagc aaaaggagcc
     3061 gaccagcttt ctccatatta tggaatcaag ccaagtgatt atatttctgg ttctcttgtc
     3121 atccataaca ctgacatcga gcaaagcaga aatgcagaaa atgaatctcc taaaggccag
     3181 gcttcctcaa atgggtgtgc tgcgctgaag aaagattctc tgccattgtt gcccaaaaat
     3241 cgaggaatgg taatagtgaa tggtggactg aaacaagatg agagacctgc tgccaaccca
     3301 cagcaagaga acatttccca gaatcctcag cacgaagacg accacaaatc tccctcgtgg
     3361 atctctgaga acccattagc tgccaatgag aatgtctcac caggaattcc ctcagcagag
     3421 gaacagttgt ctagtatagc aaaaggtgtg aatggttcca gccaggctcc aaccagtggg
     3481 aaatattgcc ggctatgtga tatccagttc aacaaccttt caaactttat aactcacaag
     3541 aagttttatt gctcatcaca tgcagcagaa catgtcaaat gaaacccagc tttcttgtac
     3601 aaagtggttg atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     3661 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
     3721 tcagcctcaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3781 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3841 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3901 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3961 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4021 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4141 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4201 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4261 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4321 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     4381 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4441 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     4501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4561 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4621 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     4681 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     4741 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4801 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     4861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5041 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5101 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5161 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5221 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5281 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5341 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5401 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5461 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5521 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5581 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5641 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5701 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5761 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5821 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5881 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5941 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6001 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6061 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6121 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     6181 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     6241 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     6301 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     6361 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     6421 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     6481 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     6541 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     6601 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6661 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6721 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6841 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6961 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7081 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7141 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7981 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8041 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9061 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9121 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9181 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9241 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     9301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9841 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     9901 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9961 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10021 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    10081 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    10141 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    10201 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    10261 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    10321 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    10381 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10441 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10501 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10561 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
    10621 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10681 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10741 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10801 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    10861 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    10921 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    10981 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    11041 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    11101 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    11161 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    11221 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    11281 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    11341 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    11401 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
    11461 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    11521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    11581 agaa
//
