LOCUS       sequence-389114-       19590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of wt 3xFLAG-BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19590)
  AUTHORS   Hunkeler M, Jin CY, Fischer ES
  TITLE     Structures of BIRC6-client complexes provide a mechanism of 
            SMAC-mediated release of caspases.
  JOURNAL   Science. 2023 Mar 17;379(6637):1105-1111. doi: 
            10.1126/science.ade5750. Epub 2023 Feb 9.
  PUBMED    36758104
REFERENCE   2  (bases 1 to 19590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade5750"; journalName: "Science"; date: 
            "2023-03-17- 17"; volume: "379"; issue: "6637"; pages: "1105-1111"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG_BIRC6
FEATURES             Location/Qualifiers
     source          1..19590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(18017..18037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18435..18446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(18513..18530)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    18519..18726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(18774..18779,18780..18796,18797..18802)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             18991..19524
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc ctcgcgaacc atggtaacgg gtgggggtgc
     3361 ggcaccaccc ggcacggtca ctgagccgct gccatccgtg atagtgcttt ctgccggtcg
     3421 aaagatggcc gcagctgctg ccgccgcctc cggacctggt tgttcctctg ccgcaggagc
     3481 tggggctgcg ggggttagcg aatggctggt actgcgagat ggctgcatgc attgcgatgc
     3541 agacgggctg catagtctct cctaccaccc agcgcttaat gctattctgg cggttacctc
     3601 ccgcggcacg atcaaggtca ttgatggaac aagtggggca acgctccaag cctccgcgct
     3661 gtcagccaaa ccaggcggac aggttaaatg ccagtacatt tcagcagtag ataaggttat
     3721 tttcgtcgat gactatgccg tggggtgtcg gaaagatttg aatgggatac tcctgctgga
     3781 tacggcgctc cagaccccgg tcagtaagca ggacgacgtt gtccagctgg aactccccgt
     3841 gaccgaagca cagcaattgc tctctgcctg cctcgagaaa gtagatatca gcagcaccga
     3901 aggttacgac ctcttcatta cgcagcttaa ggatggcctg aagaatacca gccacgagac
     3961 agcagcgaat cacaaggtag ctaaatgggc gaccgtaaca ttccaccttc ctcaccacgt
     4021 gcttaaatct atagcttctg ctatagtcaa cgagctgaaa aaaataaatc agaacgtcgc
     4081 tgcccttcca gtggctagtt cagttatgga tcgcttgagc tacctcttgc ctagcgcgcg
     4141 cccagagttg ggagttggcc ccgggcggtc cgtcgatcga tcacttatgt actctgaagc
     4201 taatcggaga gaaactttta cctcctggcc ccatgtcggc tatcgatggg ctcagccaga
     4261 cccgatggcc caggctggat tttaccatca acctgcgtct tcaggtgatg acagagcgat
     4321 gtgctttacc tgctcagtat gcctggtatg ttgggagccg accgatgagc cttggagcga
     4381 gcatgaacgg cattcaccga attgcccgtt tgtcaaggga gaacacacgc agaatgtacc
     4441 actctctgtt actctggcta cgtcccccgc acaatttccg tgtaccgacg gaacagatcg
     4501 aatatcatgc tttgggagcg ggtcctgtcc acacttcctt gcagcggcta ccaaacgcgg
     4561 aaaaatatgc atctgggatg tctcaaagct tatgaaagtc catttgaaat tcgaaatcaa
     4621 tgcatatgac cctgccatcg tacagcaact tatcttgagt ggcgatccct cctccggtgt
     4681 agatagccga agacctacgc ttgcgtggct cgaggattca tcatcttgct ctgacatccc
     4741 gaaactcgaa ggagatagtg atgacctgct cgaggattca gactccgaag aacacagcag
     4801 gtccgatagc gttactgggc atacctcaca aaaagaggcc atggaagtct ccctcgacat
     4861 taccgcgctt tctatcctcc aacagcctga aaaactccaa tgggaaatag tggccaatgt
     4921 gcttgaagac actgtaaaag atcttgagga actgggagcg aatccctgcc tgactaactc
     4981 caaatctgaa aaaacgaagg agaagcacca ggagcagcat aacattcctt tcccctgtct
     5041 gcttgcgggc ggacttctga catacaaatc cccagctact tcacctatta gttcaaattc
     5101 tcataggtca ctcgatggcc tttctcgaac gcagggtgaa tccattagtg agcagggttc
     5161 aacggataat gaatcctgta ctaatagcga gctcaattct cccttggtca gacgcacttt
     5221 gcctgtattg cttctgtatt ctatcaagga gagcgatgaa aaagcaggga aaatatttag
     5281 ccaaatgaac aatattatgt ccaaaagtct tcatgatgat ggcttcacgg tcccgcaaat
     5341 tatcgagatg gaactggatt cacaagaaca attgctcctt caagacccac ctgttacata
     5401 tattcaacaa ttcgcagacg ccgcagcaaa tctcacgagc cctgattccg agaaatggaa
     5461 ttcagtattc cccaagcctg gtacgctggt tcagtgtttg cgactgccta aattcgcgga
     5521 agaagagaat ctttgcatag actcaataac gccttgtgca gatggtattc atttgctggt
     5581 gggactgcgc acgtgcccag tcgagagcct ctcagcaata aaccaagttg aagctttgaa
     5641 caacctgaac aagctgaata gcgcactgtg taatagacgg aaaggcgaac tcgaatccaa
     5701 tttggcggtt gtcaacggtg caaatatctc cgttatccaa catgaatccc ccgctgatgt
     5761 tcaaactccc ctcataattc agcctgaaca aaggaacgta agtgggggat atttggtgct
     5821 ctacaaaatg aattacgcta cacgcatagt aactcttgag gaagagccca taaagattca
     5881 acatatcaaa gacccgcaag acacgatcac atcactcatt ctcctccccc ccgacatcct
     5941 cgacaatcgg gaagacgact gtgaggaacc aatagaggac atgcaactta cgtccaaaaa
     6001 cggcttcgaa cgggagaaga cttcagacat cagtacactg ggacacctgg taattacgac
     6061 tcaaggcggc tacgttaaga tccttgattt gagcaatttc gaaatcctgg cgaaggttga
     6121 accgcccaaa aaagaaggaa cagaagagca agacacattc gtctcagtga tctactgtag
     6181 tggcactgac cgcctttgcg cgtgcaccaa gggcggggaa cttcattttc tgcagatagg
     6241 aggcacatgt gacgacatag acgaggccga catattggtc gatgggagcc ttagtaaggg
     6301 gatagaaccg tcttctgagg gctccaagcc ccttagcaac ccttccagtc cgggtatctc
     6361 tggcgttgac ctgttggtag atcaaccgtt cacgctcgaa atcttgactt cactggtgga
     6421 gctgactcga tttgagaccc tgactccaag attctccgca actgttcctc cctgttgggt
     6481 agaagtccag caagagcagc agcagcgacg gcacccacaa cacctccacc aacaacatca
     6541 cggggatgcc gcgcaacata cgagaacgtg gaaactgcag accgattcaa acagttggga
     6601 cgaacacgta ttcgaattgg tcctccctaa ggcatgcatg gttggacatg tcgacttcaa
     6661 atttgtcctc aatagtaaca ttactaatat tccgcaaatt caagttactc tgctgaaaaa
     6721 caaggcaccg gggttgggaa aagtcaatgc gttgaatata gaagtcgagc aaaacggaaa
     6781 accctcattg gttgacctta atgaagagat gcaacacatg gatgttgagg aaagtcagtg
     6841 cctcagactt tgtcccttcc ttgaagacca caaagaagat attctctgcg gtcccgtttg
     6901 gttggcctcc ggcctggact tgtccggcca cgcagggatg cttacactta cgagtccaaa
     6961 gctggtgaaa gggatggcgg gaggcaagta caggtctttc ctcatacacg ttaaagcagt
     7021 aaacgaacgg gggactgagg aaatatgcaa cgggggtatg aggccagtcg ttcgcctccc
     7081 ttcactcaaa catcagagca acaaaggcta ctccctcgct agtttgctcg caaaagtggc
     7141 tgcggggaag gagaaatctt ccaatgttaa gaatgagaac accagtggca ctcgcaaatc
     7201 tgaaaatctt cgaggctgcg acctcttgca ggaagtttca gtgacaatca ggcgattcaa
     7261 aaaaacttcc atatctaagg agagagtaca acgctgtgct atgttgcaat ttagcgaatt
     7321 tcatgaaaag ctcgttaata cactgtgtcg aaaaacggat gatgggcaga taaccgagca
     7381 tgcgcagtct ctggtccttg acacactctg ctggctggcg ggggtacact ccaatggccc
     7441 aggtagctcc aaggaaggta acgaaaatct cctgtcaaaa acccggaaat tcctgagtga
     7501 tattgtgcgc gtctgctttt tcgaagccgg gcgaagtata gcacacaaat gcgctaggtt
     7561 tctcgcgctc tgtatctcaa acggtaagtg cgatccttgc caacccgcgt tcggtccagt
     7621 ccttttgaag gccctcctcg ataacatgtc atttctgccg gctgccacga ctgggggctc
     7681 agtgtactgg tattttgttc ttctgaacta cgtcaaggat gaagatcttg ctggctgcag
     7741 tacggcctgt gcctcacttt tgactgctgt ctcccgccaa ctgcaggatc gcttgacacc
     7801 gatggaagcg cttctccaga cacgatatgg actctactcc tctccgttcg acccagtgct
     7861 gttcgacctt gagatgtctg ggtcttcatg caagaatgta tataacagca gcataggggt
     7921 ccagtctgac gaaatagatt tgtccgacgt actcagtgga aacggtaagg tttcaagttg
     7981 tacggcggct gaaggctcat ttactagcct taccggactt cttgaagtcg agccgctcca
     8041 tttcacatgt gtttcaacat ctgacgggac gcggattgag cgagatgatg ctatgagtag
     8101 cttcggtgtg acaccggcgg tgggagggct ctcctcagga acggtagggg aggcatcaac
     8161 ggctctgagt tccgctgcac aggtggctct gcagtctctg tcccatgcca tggccagtgc
     8221 ggagcagcaa ctccaggttc tgcaggaaaa gcagcaacaa cttcttaagc tgcaacaaca
     8281 gaaggcgaag ttggaggcga aactgcacca aacaaccgca gcggcagccg cagctgcgtc
     8341 agctgtcggc cccgtacaca atagcgtccc tagcaacccc gtcgctgccc cgggcttctt
     8401 tatacaccca agcgatgtga tcccgcctac tccaaaaacg acacccctgt ttatgactcc
     8461 acccttgacg ccgccaaatg aagctgtttc tgtcgtcatc aacgcagagt tggcccagct
     8521 tttcccgggt tcagttattg acccgcctgc cgtcaatctc gcagcacaca acaaaaactc
     8581 aaacaaatcc cgaatgaacc ctttggggtc aggacttgct cttgccatct cacacgctag
     8641 ccacttcctt cagccccctc cccatcaatc catcataatt gaacggatgc atagtggagc
     8701 tagacgcttc gttacgttgg atttcggaag gccaatcctc ctgacagacg ttcttatacc
     8761 cacctgtggc gacttggcta gcctttccat tgatatatgg accctgggtg aagaggttga
     8821 cggtcgcaga ctggttgtag caacagacat tagcactcac tccctgattt tgcatgatct
     8881 cattccgccc cccgtatgtc gcttcatgaa aattacagtc atcggccggt atggttcaac
     8941 gaatgcaagg gcgaagatac cactcggttt ttactatgga cacacgtaca ttcttccttg
     9001 ggagtccgag cttaagttga tgcatgaccc attgaaaggc gaaggggaat ctgccaatca
     9061 gcccgagatt gatcagcacc ttgccatgat ggtggcattg caagaagaca ttcagtgtcg
     9121 ctacaatctg gcttgtcacc gcttggaaac cttgctccag tccattgatc ttccaccatt
     9181 gaatagcgct aacaacgcgc agtacttcct gaggaaaccc gacaaagctg tcgaggaaga
     9241 ttctagggtc ttctctgcat accaggactg tattcaactt cagcttcagc tcaaccttgc
     9301 ccacaatgct gttcaacgcc tgaaggttgc cttgggggcc tcaagaaaga tgctctctga
     9361 aacaagtaat cccgaagacc tgatacagac aagctccaca gaacaactgc gaacgatcat
     9421 acggtacttg ctggacacgc ttctctcctt gttgcacgca agcaatggac attccgtgcc
     9481 agcagtcctg cagagcacat tccatgcgca agcttgtgag gaactcttca agcatttgtg
     9541 tatttcaggg acaccaaaga ttaggcttca cacagggctt ttgctggtgc aattgtgcgg
     9601 cggggagagg tggtggggtc aatttctttc caacgttttg caggagcttt ataattctga
     9661 acaacttttg atatttccgc aagaccgggt gttcatgttg ctcagctgca ttggacagcg
     9721 aagcttgtct aatagcggtg tactcgaatc cctgctgaac ctcttggata atttgctttc
     9781 cccgttgcag ccacagctcc ccatgcaccg acggactgaa ggggttcttg acatcccaat
     9841 gatatcatgg gtcgtaatgt tggtcagtcg gttgcttgac tatgtggcaa ccgtagagga
     9901 tgaagcagcc gcagcaaaaa aacctttgaa tggcaatcag tggtctttca taaacaataa
     9961 tcttcataca caatccttga accgctctag caaagggtca agtagcctgg atagattgta
    10021 ctcaagaaaa atccggaaac aactcgttca tcataagcag cagcttaacc tcctcaaggc
    10081 gaaacaaaag gctctggttg agcagatgga gaaagaaaaa attcagtcaa ataagggatc
    10141 tagctataag ttgctcgttg agcaggccaa acttaagcaa gcaacgagta agcattttaa
    10201 agatctcatt aggctgagac ggacggccga gtggagtaga agtaatcttg atacagaagt
    10261 taccacggcc aaggagtctc cggaaattga accactgcct ttcactttgg cccatgaaag
    10321 gtgcattagc gtggttcaaa agcttgttct tttcttgttg tctatggatt tcacatgtca
    10381 tgccgacctc ttgctcttcg tttgtaaggt cttggcacga attgctaatg cgaccaggcc
    10441 aacaatacac ttgtgcgaaa ttgtaaatga accccaactg gaaagattgc tgctcttgtt
    10501 ggtcgggacg gacttcaacc gaggcgacat cagctggggc ggcgcctggg cccaatactc
    10561 attgacttgt atgctccaag atatactcgc aggagagttg cttgctccgg tggccgccga
    10621 agcgatggaa gaagggaccg ttggtgatga tgttggagct acggcggggg attccgatga
    10681 tagtctgcaa caatcatcag ttcagcttct ggagaccatt gacgaacccc ttacccacga
    10741 catcactggt gcaccccccc tgtcatcact tgagaaagac aaagagatag atctcgagct
    10801 tctccaggac cttatggaag tagacatcga tccactcgac atagacctgg agaaggaccc
    10861 attggcggca aaggtattca aacccatctc atctacttgg tacgattatt ggggcgcgga
    10921 ctacggtaca tataactata atccttacat aggtggactt ggaatcccag ttgcgaaacc
    10981 tccagcgaat actgagaaga acggtagtca gactgtatcc gtgtccgttt ctcaagccct
    11041 ggatgctcgc ctggaagtgg gtttggagca gcaggcagag ttgatgttga aaatgatgag
    11101 cactcttgaa gcggacagta tattgcaggc tctgaccaat acgtcaccta ccttgtctca
    11161 gagcccaact gggaccgatg attcactcct tggtgggctt caagctgcga atcaaaccag
    11221 ccagctgatt atacagctct catctgtacc tatgctgaac gtttgtttca acaaactttt
    11281 ctccatgctc caggttcacc acgtgcagct tgagtccttg ctccaactgt ggcttacact
    11341 ttccctgaat agttcctcta caggcaataa ggaaaatggt gcagatatct ttctgtacaa
    11401 tgccaataga ataccggtga ttagtcttaa tcaagcctct atcaccagct tcctcaccgt
    11461 gctggcgtgg tatcccaaca cacttttgcg cacctggtgc ttggttttgc atagtctgac
    11521 cctgatgaca aatatgcaat tgaattcagg tagttccagc gctataggaa ctcaggaatc
    11581 tacggcacac ctgttggtgt cagatccaaa cctgatccac gtactggtta aattcctctc
    11641 tggtacttcc ccgcatggta cgaaccaaca cagcccacaa gttggaccta cagccaccca
    11701 ggcgatgcag gagtttttga ccagactcca ggtacacctt tcttcaacct gccctcagat
    11761 attctcagaa ttcctcctca agcttattca tatcctcagt accgaaagag gggcgttcca
    11821 gacggggcaa ggtcctttgg atgctcaagt caagctgttg gagttcaccc ttgaacaaaa
    11881 cttcgaggtt gtaagtgtct ctactatatc tgctgtcatc gaaagcgtta cgttccttgt
    11941 acaccattat atcacatgct cagataaggt catgtcaaga agtggctcag acagtagtgt
    12001 gggcgctcgg gcttgttttg gtggtctctt cgctaatctg atccgacctg gagacgcaaa
    12061 agcagtgtgc ggagagatga ccagagatca gttgatgttc gatcttctga agctcgtcaa
    12121 catccttgtg caactgcctt tgagtggcaa tagggaatac agtgctagag taagcgtcac
    12181 aactaatacg acggattccg tttccgacga agaaaaagtg tccgggggta aagatggaaa
    12241 tggttccagc accagcgtac aaggatcccc agcctacgtg gcggatctcg tgctcgccaa
    12301 tcaacagata atgagtcaaa ttctgtccgc actcggcttg tgcaattcct ctgcaatggc
    12361 catgattata ggagcttctg ggttgcattt gactaagcat gaaaactttc atggcggttt
    12421 ggatgcgatc tctgtgggag atggactttt cacgattctg actactttga gcaagaaagc
    12481 gtctacggtt cacatgatgc tgcagccgat cttgacatat atggcatgtg gatacatggg
    12541 ccggcagggg tcacttgcca catgccagtt gtctgagcct ctgctgtggt tcatactccg
    12601 agtcctcgat acttctgacg cgcttaaagc cttccatgac atgggaggag tgcagctcat
    12661 ctgcaataac atggttacaa gcactagagc gattgtcaac acagcaagat ctatggtctc
    12721 caccattatg aagttccttg actctgggcc gaacaaggca gtcgattcta ccttgaagac
    12781 gaggatcctt gcctctgagc cagataatgc tgaaggaata cacaattttg cacctttggg
    12841 tacaattacg agctcttctc caacggcaca gccggcggaa gtcctgcttc aagccactcc
    12901 tcctcaccgg agggccagat ccgcagcttg gtcatatata ttcttgccag aggaagcatg
    12961 gtgcgatctc actattcatc tccctgccgc ggtgctcctt aaggaaatcc atattcagcc
    13021 tcacctggct tctttggcta cgtgtccatc ctctgtatca gtcgaggtta gtgcggatgg
    13081 agtaaatatg ctcccgctca gtactccggt cgtaacttca ggacttacct acataaagat
    13141 ccaacttgta aaagctgagg tagcttccgc ggtctgcttg cgcttgcacc gcccccgcga
    13201 cgcttccacg ctcgggcttt cccagattaa actcctgggg cttacagcgt ttgggaccac
    13261 atcttctgct acggtaaata atccgttttt gccttccgaa gatcaagtta gcaaaacatc
    13321 tatcggatgg ctccggctcc tccatcactg tctcacgcat atctcagatc tggaaggaat
    13381 gatggcttca gcggctgccc ccacggccaa tcttctccag acttgcgccg cacttctcat
    13441 gtccccctat tgcggaatgc actcacccaa tattgaggtc gtcttggtta aaatcggcct
    13501 ccagtcaact cggataggtc tcaagcttat agacatcttg ttgcggaatt gtgcagctag
    13561 tggttctgat cctacagatc tgaacagtcc actgttgttt ggtcggttga atggcctctc
    13621 tagcgatagt acaatagaca ttttgtatca acttggaact acccaagatc cgggaaccaa
    13681 agatcggatt caggcattgc tcaaatgggt aagcgatagc gcgcgcgtag cagctatgaa
    13741 aagatctggc cgaatgaatt acatgtgccc gaattccagc actgtagaat acgggcttct
    13801 gatgccgtca ccgagtcacc tgcattgcgt tgcggcaatt ctgtggcact cctatgagtt
    13861 gctcgttgaa tacgacttgc cagcactgct ggatcaggag ctctttgagc ttctgtttaa
    13921 ttggtccatg tctctgccgt gtaatatggt tctcaaaaag gctgttgaca gcttgctttg
    13981 ctctatgtgt cacgtacatc ctaactactt ctctctgttg atgggttgga tgggcataac
    14041 ccccccgccg gttcaatgcc atcaccgctt gtccatgaca gatgacagca aaaagcaaga
    14101 cttgtcttca tctctgacag atgattctaa aaatgcccaa gcacccttgg cgctgactga
    14161 aagccacttg gctaccctcg caagttcttc acagtctcct gaagctatta agcagctcct
    14221 ggacagcggg ctgccgagcc tccttgttcg gagcttggct tcattctgtt tttcccacat
    14281 aagtagtagt gaatccattg cacagagcat tgacatttca caggataaac tccggcggca
    14341 tcatgttccc cagcaatgta acaaaatgcc gattacggcg gatctcgtcg ccccgatatt
    14401 gcggttcctt accgaggtcg gtaacagcca catcatgaaa gactggctgg gtggttccga
    14461 agtcaatcct ctctggactg cactgctgtt cttgttgtgt cattctggga gtaccagcgg
    14521 ctcacacaat ctcggggccc agcagacgag cgctaggtct gccagcttga gttctgccgc
    14581 tactacgggt cttacaacac aacaaagaac cgctatagaa aacgcgacag tggcgttttt
    14641 tctccagtgc ataagttgcc acccaaataa tcaaaagctt atggctcaag tgctttgcga
    14701 gctctttcag accagtccac agaggggcaa tctcccgacg tctggcaaca taagcggttt
    14761 tatcagacga ctcttccttc aactcatgct ggaggacgaa aaggtcacta tgttcctgca
    14821 gagcccgtgc ccgctctata agggaaggat aaatgccacg agccatgtca ttcagcaccc
    14881 gatgtacggg gccgggcaca aatttagaac actccacctg ccagtatcta cgacgctttc
    14941 agatgtcctt gatagggtct ccgatactcc gagcatcacc gccaagctta tctccgagca
    15001 gaaagatgac aaagaaaaaa aaaaccacga ggaaaaagag aaagtcaaag ccgaaaatgg
    15061 ctttcaggat aactactccg tggtagtcgc ctccgggctc aagagtcaaa gcaagagggc
    15121 ggtcagcgcg acccctcctc gaccaccatc acgccgcggg aggacgatac cggacaagat
    15181 cggaagcacg agcggcgccg aggcagcgaa taaaatcatc acggtaccag tgtttcatct
    15241 gttccataag cttctcgcag gtcagccact gccagcggaa atgactctgg ctcaactgct
    15301 caccctgctt tatgaccgca agttgcctca ggggtacaga tctatagatc ttacggttaa
    15361 gctgggcagt cgggtgatta cagacccttc actgtccaaa actgattcct ataagcgctt
    15421 gcaccctgaa aaggatcacg gtgacctcct tgcttcctgc ccagaggacg aggccttgac
    15481 acccggcgac gaatgcatgg acggaatttt ggatgaaagc ctgctggaaa cctgtccgat
    15541 acaatccccg ctccaggtat tcgccgggat gggtggtctt gctctcatcg ccgagaggtt
    15601 gcccatgctt tatcctgaag tgatccagca ggtatcagct ccggttgtaa cctcaaccac
    15661 ccaagagaaa cctaaagatt cagatcaatt tgaatgggtg acaattgagc agagcggcga
    15721 attggtgtat gaggcccccg aaacggttgc tgccgagccg cccccaataa aatccgccgt
    15781 ccagacaatg tctccaatac cagcgcactc tttggcggca tttgggcttt ttcttaggct
    15841 ccctggttac gctgaagtcc tgctgaaaga acgcaagcat gcgcaatgcc ttctgcggct
    15901 cgttttgggg gtgacggacg atggagaagg ttcccatatc cttcagagcc ccagcgcgaa
    15961 cgtattgccc acacttcctt ttcatgtgct ccgatccctc ttttcaacta cccccctcac
    16021 aactgatgac ggcgtccttc tgcgacgaat ggcccttgag atcggggcct tgcacctcat
    16081 tctcgtgtgc ctcagtgcgt tgagccacca cagtccccgg gtcccaaaca gcagcgtgaa
    16141 ccagacggaa ccacaggtga gcagctccca caaccccacg agtaccgagg agcagcagct
    16201 ctactgggca aaagggaccg gatttgggac tggctcaacc gcctccggat gggatgtgga
    16261 acaagctctt acgaagcaaa ggttggagga ggaacatgta acttgtctgc tccaggtgct
    16321 tgcgagctat ataaaccctg tgtcttcagc ggtgaatggc gaggcacaat ccagtcacga
    16381 aacgcgcggt cagaattcca atgctctccc cagtgtgctc ctggagcttc ttagccagag
    16441 ttgtttgata cctgccatga gttcatacct tcgaaacgac tctgtgctgg acatggcgag
    16501 acatgttccc ttgtatagag ctcttctgga actgctgaga gctatagcca gttgcgctgc
    16561 catggtgcct ctcctgctcc ccctgagcac cgagaatggc gaggaggagg aggagcagag
    16621 cgagtgccag accagtgttg gcactctcct tgccaaaatg aaaacttgtg tcgatactta
    16681 taccaaccgc ctgcggtcta agagagagaa tgttaaaacc ggagttaagc cagacgcatc
    16741 tgaccaggaa cccgaagggc ttactttgct cgtaccagat atacagaaaa cagctgaaat
    16801 tgtttacgct gcaacaacat cactccgaca ggccaaccag gagaagaaac ttggggagta
    16861 ttcaaagaaa gcggccatga agcctaagcc gctgtccgtc ctgaaatccc ttgaggaaaa
    16921 gtatgtcgca gtgatgaaaa agctccaatt cgacactttt gaaatggtgt ctgaagacga
    16981 ggacggcaag ctgggattca aagtgaacta tcattatatg agccaggtca aaaacgctaa
    17041 cgatgcaaat agtgcagcta gggctcggcg ccttgctcaa gaagcggtta ccctcagtac
    17101 tagtctcccc ttgtcatcca gtagttcagt cttcgtgaga tgtgatgaag agagattgga
    17161 tattatgaaa gttctgatca ccggccccgc agacactcct tatgcaaacg gatgcttcga
    17221 gtttgacgtt tacttcccac aggattaccc ttccagcccg ccccttgtca atttggaaac
    17281 taccgggggt cactccgtac gcttcaaccc caacctgtat aacgacggca aagtttgcct
    17341 ctcaattctg aatacatggc atggaagacc cgaagaaaaa tggaacccgc aaacatcatc
    17401 atttctccag gtactcgtgt ccgttcaatc attgatcctc gtggcggaac cttactttaa
    17461 cgagcctggt tacgagaggt ctcgaggcac accatctggt actcaatcca gcagggagta
    17521 cgatggcaat atccgccaag ctacagtgaa gtgggctatg ctcgaacaga ttaggaatcc
    17581 ctctccttgc ttcaaggagg tgatacataa acatttctat ttgaaaagag tagaaattat
    17641 ggcgcagtgt gaagagtgga tagcagacat ccaacaatat tcctcagaca aacgggtcgg
    17701 acggactatg tctcatcacg cagcggctct caaacgacat acggcccaac tccgcgagga
    17761 actcttgaaa ttgccgtgtc ctgagggttt ggatcctgac acggacgacg ccccagaagt
    17821 atgtagagcc actacaggtg cggaagagac ccttatgcac gatcaggtaa aaccctcaag
    17881 ttcaaaggaa cttccgtccg actttcagct ttgagcggcc gctgatgata atggcctgtg
    17941 gccgtaatgg cctgtggccg taaaatcaac ctctggatta caaaatttgt gaaagattga
    18001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
    18061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
    18121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    18181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    18241 ggactttcgc tttccccctc cctattgcca cggcggaact cagtgccgcc tgccttgccc
    18301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
    18361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    18421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    18481 ctctgcggcc tcttccgcgg cttcgagatc tgcctcgact gtgccttcta gttgccagcc
    18541 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    18601 cctttcctaa taaaatgagg aaattgcatc aaattgtctg agtaggtgtc attctattct
    18661 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gcagacaata gcaggcatgc
    18721 tggggactcg agttaagggc gaatcgctgg gacgcccgaa cggtggctac tcgttgacat
    18781 aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
    18841 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
    18901 actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
    18961 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagcaacga tgttacgcag
    19021 cagggcagtc gccctaaaac aaagttaggt ggctcaagta tgggcatcat tcgtacatgt
    19081 aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt cggtcgtgag
    19141 ttcggtgatg tagccaccta ctcccaacat cagccggact ccgattacct cgggaacttg
    19201 ctccgtagta agacatttat tgcgcttgct gccttcgacc aagaagcggt tgttggcgct
    19261 ctcgcggctt acgttctgcc caagtttgag cagccgcgta gtgagatcta tatctatgat
    19321 ctcgcagtct ccggcgagca ccggaggcag ggcattgcca ccgcgctcat caatctcctc
    19381 aagcatgagg ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga ttacggtgac
    19441 gatcccgcag tggctctcta tacaaagttg ggcatacggg aagaagtgat gcactttgat
    19501 atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt cccggccgcg
    19561 gagttgttcg gtaaattgtc acaacgccgc
//
