LOCUS       sequence-38913-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-TGG-F3-GGG
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..102
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atctgccacc atggctccaa gaagaagcgt aaggtagact acaaagacca tgacggtgat
       61 tataaagatc atgacatcga ttacaaggat gacgatgaca agggtaccca gccctataaa
      121 tgtccggagt gtggcaagtc cttctcgacc tctggcaacc tcacccgtca ccaacgtacg
      181 cacaccggcg aaaaacctta tgcttgccct gtcgagtcct gtgaccgccg cttcagtcgc
      241 tctgaccacc tcaccaccca tatccggatt cacacaggcc agaagccctt ccagtgtcgc
      301 atctgcatga ggaacttcag cgacccgggt cacctcaccc gtcacatccg cacccacaca
      361 ggcgagaagc cttttgcatg cgacatctgc ggcaggaagt tcgcgcgctc tgaccatctc
      421 acccgtcaca ccaagatcca cacaggtgga tcccaactag tcaaaagtga actggaggag
      481 aagaaatctg aacttcgtca taaattgaaa tatgtgcctc atgaatatat tgaattaatt
      541 gaaattgcca gaaattccac tcaggataga attcttgaaa tgaaggtaat ggaatttttt
      601 atgaaagttt atggatatag aggtaaacat ttgggtggat caaggaaacc ggacggagca
      661 atttatactg tcggatctcc tattgattac ggtgtgatcg tggatactaa agcttatagc
      721 ggaggttata atctgccaat tggccaagca gatgaaatgg aacgatatgt cgaagaaaat
      781 caaacacgaa acaaacatct caaccctaat gaatggtgga aagtctatcc atcttctgta
      841 acggaattta agtttttatt tgtgagtggt cactttaaag gaaactacaa agctcagctt
      901 acacgattaa atcatatc
//
