LOCUS       sequence-389132-        6624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle and expression vector. Replicates in E.coli and 
            Mycobacterium. Gives acetamide-inducible gene expression in 
            Mycobacterium. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6624)
  TITLE     Coleman Lab plasmids
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUS116
FEATURES             Location/Qualifiers
     source          1..6624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3689..3708)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3700..4515
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3773..3792)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4846..5434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5335..5354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac
       61 aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg
      121 ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg
      181 gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta cagcgacggc
      241 tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga ccgccgccga aggcgctcga
      301 atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg cgcaggaacg cagcgagtgg
      361 ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct atcacgacga cgagggccac
      421 tcttggccgc aaacggccaa acatttcggg ctgcatctgg acaccgttaa gcgactcggc
      481 tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg ctcaaaaggc ccacaacgaa
      541 gccgacaatc caccgctgtt ctaacgcaat tggggagcgg gtgtcgcggg ggttccgtgg
      601 ggggttccgt tgcaacgggt cggacaggta aaagtcctgg tagacgctag ttttctggtt
      661 tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc agccagacga
      721 gacggggttc tacgaatctt ggtcgatacc aagccatttc cgctgaatat cggggagctc
      781 accgccagaa tcggtggttg tggtgatgta cgtggcgaac tccgttgtag tgcctgtggt
      841 ggcatccgtg gcgcggccgc ggtaccagat ctttaaatct agataaagaa gtgacgcggt
      901 ctcaagcgtc gagcgtcgcc agcgtgtcga ggatgtcgaa gtcgtagccg tcggcgctgg
      961 cgatgtagac ctgctggtcg aattgactgt cgcgcataca catcgggccc cggggcccgt
     1021 cgaacccgac atcgtgcgcg gatgccatca ggtccggtat ctcgggggag tgggcccgct
     1081 ggaagatggc ctcgagcgca agcagaccct cgtaacagga ttcggccatc gcgttgagcg
     1141 gtggcgcgtc ggcgccgtag cgggcgacgt agctgcccat caggtccatg gcacccgcgg
     1201 tggccagtga actgaagtac gccgcggcga catagaggtt ttcggtggag ccggcgccgc
     1261 tggccagcag catgttctcc tccatcagcg ggctgaaccg cgccatgcgg tcgtgcccgc
     1321 cggcgcgcgc gaactcgcgg ttgaacaaca cggcgtcctg gccgacgagc agcatcaaca
     1381 cggcctgcgc ccccgacgcg atggccttgc ggacaggtgc gcggaaatcg tcggtgccgt
     1441 acgggacgta gatctcccgt ctgagctcga ggtccagatc tcggcagtac gcgcgggcgg
     1501 ccgcggcgga acggcgcggc cagatgtagt catcgccgac caggcaccag gaccggatgc
     1561 cgaagtggtc gcgcagccag gcgagcgcgg gcgcgatctg gatctgcggt gtctcgcctg
     1621 tgcagaacac gcccggtgtg cgttcaccgc cctcgtacaa cgaggtgtag acgtacggga
     1681 tgcggtcgcg gaccaccggg gagatgcggt tgcgcacggc cgagatgtgc cagccggtca
     1741 cggcgtcgag accgtgacct cgcaaccggt cggcgacggt ccgggcgacg tcgtcgccgg
     1801 gcgctccgcc gtcgagcacc tcgatggtga ccttgcggcc ctgcaggccg cctcggtcgt
     1861 tgacctcctt ggccgcgagc tcggccacgg cctcgcacga aggcgcgaag attcccgctg
     1921 gcccttgaag cggaatcacc agcccgacgc ggaactcaac ctcgccgtcc tgcactccag
     1981 atcaccgtcg atcccgtgta gtctgcgctt caaagctttc tagcagaaat aattcattct
     2041 gaacagaccc cgccgtcgac acgaggagac acccaccatg gccgccggac agcagcgccg
     2101 ccccaacctc ctgctgccgt tggtgcgtct gacccacctc gcggagtcgg cgatcgaacg
     2161 cgtgctcgcg gactcgtcgc tcaagatcga ggactggcgc gtgctcgacg agttggccgg
     2221 acggcgcacc gtgcccatga gcgatctcgc gcaggccacg ctgatcacgg gtccgactct
     2281 caccagaacc gtcgatcgcc ttgtgtcgca agggatcatc taccggactg ccgatctgca
     2341 tgaccgccgg cgggtgctcg tggcgttgac cccgcggggg cggacgctgc gcaaccgcct
     2401 ggtggacgcg gtagccgagg ccgagtgtgc ggcttttgaa tcgtgcgggc tggacgtcga
     2461 ccagttgcgc gaactcgtcg acaccacctc gaatttgact tcgtaaccac ccgcgcccgg
     2521 cgcgggcgtt cacccttgac ttttattttc atctggatat atttcgggtg aatggaaagg
     2581 ggtgaccatg ccgacctaca cattccgttg ttcccactgc ggtcccttcg atctcacctg
     2641 cgcgatctcc gagcgcgatg cggcggcgac ctgtccggag tgccggacgc cggcgcgccg
     2701 ggtcttcggt tcggtagggc tgacgacatt caccgcggga catcaccgcg cattcgacgc
     2761 ggcgtccgcg agcgccgaaa gtcccacggt ggtgaagtcg attcccgcag gcgcggaccg
     2821 cccgcgggcc ccgcgccgca atcccggtct accgagtctg ccgaggtact agcgacatgg
     2881 gtggcgtcgg gctcttctac gtgggtgcgg tgctcatcat cgacgggctg atgctgctgg
     2941 gccgcatcag cccacgaggc gcaacaccgc tgaacttctt cgtcggcgga ctgcaggtgg
     3001 tgacgcctac ggtgctgatc ctgcagtccg gcggagacgc ggccgtgatc ttcgcggcct
     3061 ccgggctcta cctgttcggc ttcacctacc tgtgggtggc catcaacaac gtgaccgact
     3121 gggacggaga aggtctcgga tggttctcgc tgttcgtcgc gatcgccgca ctcggctact
     3181 cgtggcacgc gttcaccgcc gaggccgacc cggcgttcgg ggtgatctgg ctgctgtggg
     3241 cagtgctgtg gttcatgctg ttcctgctgc tcggcctggg gcacgacgca ctggggcccg
     3301 ccgtcgggtt cgtcgcggtg gccgaaggcg tgatcaccgc cgccgtgccg gccttcctga
     3361 tcgtgtcggg caactgggaa accggcccgc tccccgccgc ggtcatcgcc gtgatcggtt
     3421 ttgccgcagt tgttctcgca taccccatcg ggcgccgtct cgcagcgccg tcagtcacca
     3481 accctccacc ggccgcgctc gcggccacca cccgataaga gaaagggagt ccacatatgt
     3541 aacggatcca gctgcagaat tcgctagcca cctgacgtcg ggggggggga aagccacgtt
     3601 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     3661 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     3721 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     3781 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     3841 catgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     3901 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     3961 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
     4021 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     4081 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     4141 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     4201 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa tcttttgcca
     4261 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     4321 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     4381 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     4441 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     4501 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     4561 acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     4621 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     4681 actggtccac ctacaacaaa gctctcacca accgtggctc cctcactttc tggctggatg
     4741 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcact
     4801 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4981 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     5281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5581 cgagcgcaac gcgtgagccc accagctccg taagttcggg cgctgtgtgg ctcgtacccg
     5641 cgcattcagg cggcaggggg tctaacgggt ctaaggcggc gtgtacggcc gccacagcgg
     5701 ctctcagcgg cccggaaacg tcctcgaaac gacgcatgtg ttcctcctgg ttggtacagg
     5761 tggttggggg tgctcggctg tcgctggtgt tccaccacca gggctcgacg ggagagcggg
     5821 ggagtgtgca gttgtggggt ggcccctcag cgaaatatct gacttggagc tcgtgtcgga
     5881 ccatacaccg gtgattaatc gtggtctact accaagcgtg agccacgtcg ccgacgaatt
     5941 tgagcagctc tggctgccgt actggccgct ggcaagcgac gatctgctcg aggggatcta
     6001 ccgccaaagc cgcgcgtcgg ccctaggccg ccggtacatc gaggcgaacc caacagcgct
     6061 ggcaaacctg ctggtcgtgg acgtagacca tccagacgca gcgctccgag cgctcagcgc
     6121 ccgggggtcc catccgctgc ccaacgcgat cgtgggcaat cgcgccaacg gccacgcaca
     6181 cgcagtgtgg gcactcaacg cccctgttcc acgcaccgaa tacgcgcggc gtaagccgct
     6241 cgcatacatg gcggcgtgcg ccgaaggcct tcggcgcgcc gtcgatggcg accgcagtta
     6301 ctcaggcctc atgaccaaaa accccggcca catcgcctgg gaaacggaat ggctccactc
     6361 agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc caccgccgcg
     6421 ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga attgcgcact
     6481 gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct acctgccgac
     6541 ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga
     6601 atttccgtgc aacgacgtgt gtcc
//
