LOCUS       sequence-389166-       10410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for the neuronal expression of 
            eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal 
            activity).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  TITLE     Tools for analysing neuronal cultures in vitro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-hSyn1-eNpHR3.0-iRFP713
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        492..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             708..888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    935..1060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1553..1786
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1971..2015
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2312..2429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2484..2931
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3917..4864
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    4979..5567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5032..5052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5450..5461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5570..5586)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5571..5587)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6088..6268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6294..6311)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6300..6524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6570..6998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6657..6676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6867..6888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7007..7027)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7012..7341
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7192..7327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7254..7273
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7389..7436
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7455..7829
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7959..8080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7996..8015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8050..8069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8129..8145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8129..8145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8142..8164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8153..8169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8177..8183,8184..8201,8202..8207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8222..8243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8360..8377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8531..9119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8611..8630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9290..10081,10082..10150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9913..9932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10151..10255)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10330..10349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
       61 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      901 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      961 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1021 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1081 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1141 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1201 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1261 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1321 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1381 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1441 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1501 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1561 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1621 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1681 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1741 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1801 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1861 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1921 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1981 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2041 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2101 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2161 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2221 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2281 ttctgcagac aaatggcagt ttcatccaca attttaaaag aaaagggggg attggggggt
     2341 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2401 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2461 tttggttaat ccgagtgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt
     2521 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     2581 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     2641 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     2701 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt
     2761 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     2821 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     2881 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgctgcag
     2941 ggaactagtc ctaggggtac ccaccatgac agagaccctg cctcccgtga ccgagagtgc
     3001 cgtggccctt caagccgagg ttacccaaag ggagttgttc gagttcgtgc tgaacgaccc
     3061 tttgcttgca agcagtctct atatcaacat cgcacttgca ggactgagta tactgctgtt
     3121 cgtttttatg acccgaggac tcgatgatcc acgggcaaaa cttattgctg tgtcaaccat
     3181 ccttgtgcct gtcgtcagca ttgcctccta cactggattg gcgagcggcc tgacaatttc
     3241 cgttcttgaa atgccagcgg gccattttgc agaaggcagc tcagtgatgc tgggaggaga
     3301 agaggtagat ggtgtagtca ccatgtgggg acggtatctc acctgggcac tttccacgcc
     3361 catgattctc ctcgctctgg gtctcctggc cggaagcaat gctacaaagc tcttcacagc
     3421 tatcactttc gatatcgcta tgtgcgtgac tggccttgcc gcggccctga ctacctcctc
     3481 ccacctcatg agatggttct ggtacgctat cagttgtgca tgctttctgg tggtcttgta
     3541 tatcctgctg gtggagtggg cacaggacgc caaagccgcg ggaaccgctg acatgttcaa
     3601 taccctgaag ctgttgacag tagtgatgtg gctggggtat ccaattgtgt gggctcttgg
     3661 agtcgagggt atcgcggtgt tgcccgttgg ggtgacgagc tggggatatt ctttcctgga
     3721 tatcgtggca aagtacattt tcgcattctt gctcctgaac tatctgacgt caaacgaatc
     3781 tgtcgtgtcc ggcagcattt tggatgttcc atctgcttct gggaccccgg ctgatgatgc
     3841 ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat
     3901 caacgtggtg actagtatgg ctgaaggatc cgtcgccagg cagcctgacc tcttgacctg
     3961 cgacgatgag ccgatccata tccccggtgc catccaaccg catggactgc tgctcgccct
     4021 cgccgccgac atgacgatcg ttgccggcag cgacaacctt cccgaactca ccggactggc
     4081 gatcggcgcc ctgatcggcc gctctgcggc cgatgtcttc gactcggaga cgcacaaccg
     4141 tctgacgatc gccttggccg agcccggggc ggccgtcgga gcaccgatca ctgtcggctt
     4201 cacgatgcga aaggacgcag gcttcatcgg ctcctggcat cgccatgatc agctcatctt
     4261 cctcgagctc gagcctcccc agcgggacgt cgccgagccg caggcgttct tccgccgcac
     4321 caacagcgcc atccgccgcc tgcaggccgc cgaaaccttg gaaagcgcct gcgccgccgc
     4381 ggcgcaagag gtgcggaaga ttaccggctt cgatcgggtg atgatctatc gcttcgcctc
     4441 cgacttcagc ggcgaagtga tcgcagagga tcggtgcgcc gaggtcgagt caaaactagg
     4501 cctgcactat cctgcctcaa ccgtgccggc gcaggcccgt cggctctata ccatcaaccc
     4561 ggtacggatc attcccgata tcaattatcg gccggtgccg gtcaccccag acctcaatcc
     4621 ggtcaccggg cggccgattg atcttagctt cgccatcctg cgcagcgtct cgcccgtcca
     4681 tctggaattc atgcgcaaca taggcatgca cggcacgatg tcgatctcga ttttgcgcgg
     4741 cgagcgactg tggggattga tcgtttgcca tcaccgaacg ccgtactacg tcgatctcga
     4801 tggccgccaa gcctgcgagc tagtcgccca ggttctggcc tggcagatcg gcgtgatgga
     4861 agagctgtac aagttctgct acgagaacga ggtgtaatga gactgcagtc gacaaccctc
     4921 ctgatgagag tggccccggc tgcatgagct gcaagtgtgt gctctcctga ttatcgataa
     4981 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5041 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5101 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5161 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5221 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5281 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5341 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5401 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5461 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5521 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     5581 acctcgacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5641 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcgta
     5701 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5761 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     5821 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     5881 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     5941 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6001 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6061 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6121 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6181 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6241 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     6301 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6361 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6421 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6481 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6541 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7021 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7081 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7141 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7201 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7261 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7321 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     7381 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     7441 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     7501 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     7561 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     7621 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     7681 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     7741 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     7801 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     7861 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7921 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7981 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8041 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8101 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8161 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8221 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8281 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8341 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8941 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9241 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9301 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9361 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9421 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9481 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9541 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9601 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9661 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9721 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9781 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9841 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9901 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9961 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10021 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10081 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10141 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10201 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10261 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    10321 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    10381 tgtgttggag gtcgctgagt agtgcgcgag
//
