LOCUS       sequence-389168-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for the neuronal expression of 
            membrane-bound TagBFP2 (good visualization of neuronal processes).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  TITLE     Tools for analysing neuronal cultures in vitro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-hSyn1-TagBFP2/CAAX
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        492..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             708..888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    935..1060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1553..1786
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1971..2015
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2312..2429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2484..2931
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2962..2971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2968..3669
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3780..4368
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3833..3853)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4251..4262)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4371..4387)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4372..4388)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4889..5069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5095..5112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5101..5325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5371..5799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5458..5477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5668..5689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5808..5828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5813..6142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5993..6128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6055..6074
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6190..6237
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6256..6630
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6760..6881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6797..6816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6851..6870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6930..6946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6930..6946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6943..6965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6954..6970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6978..6984,6985..7002,7003..7008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7023..7044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7161..7178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7332..7920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7412..7431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8091..8882,8883..8951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8714..8733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8952..9056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9131..9150)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
       61 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      901 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      961 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1021 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1081 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1141 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1201 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1261 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1321 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1381 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1441 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1501 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1561 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1621 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1681 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1741 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1801 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1861 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1921 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1981 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2041 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2101 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2161 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2221 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2281 ttctgcagac aaatggcagt ttcatccaca attttaaaag aaaagggggg attggggggt
     2341 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2401 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2461 tttggttaat ccgagtgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt
     2521 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     2581 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     2641 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     2701 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt
     2761 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     2821 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     2881 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgctgcag
     2941 ggaactagtc ctaggggtac cgccaccatg gtgagcgagc tgattaagga gaacatgcac
     3001 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     3061 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     3121 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     3181 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     3241 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     3301 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     3361 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     3421 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     3481 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     3541 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
     3601 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
     3661 aagcttaatt ccggactcag atctcgagct caagcttcga attctgcagt cgacaaccct
     3721 cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg attatcgata
     3781 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3841 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3901 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3961 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4021 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4141 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     4201 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4261 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4321 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     4381 gacctcgacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4441 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcgt
     4501 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4561 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     4621 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     4681 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     4741 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     4801 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     4861 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     4921 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4981 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5041 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     5101 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5161 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5221 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5281 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5341 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5521 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5581 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5641 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5701 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5761 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5821 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5881 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5941 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6001 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6061 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6121 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6181 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     6241 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     6301 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     6361 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     6421 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     6481 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     6541 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     6601 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     6661 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6721 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6781 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6841 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6901 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7021 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7141 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7741 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9061 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9121 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9181 gtgtgttgga ggtcgctgag tagtgcgcga g
//
