LOCUS       sequence-38917-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F2-GCA
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..126
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atctgccacc atggctccaa gaagaagcgt aaggtatacc catacgatgt tcctgactat
       61 gcgggctatc cctatgacgt cccggactat gcaggatcgt atccatatga cgttccagat
      121 tacgctgctc atggtacccg cccatatgct tgccctgtcg agtcctgtga ccgccgcttc
      181 agtcagtctg gtgacctgac ccgtcacatc cggattcaca caggccagaa gcccttccag
      241 tgtcgcatct gcatgaggaa cttcagccag tctggtgacc tcacccgcca catccgcacc
      301 cacacaggcg agaagccttt tgcatgcgac atctgcggca ggaagttcgc gcgcagcgac
      361 aacctctctg agcacaccaa gatccacaca ggtggatccc aactagtcaa aagtgaactg
      421 gaggagaaga aatctgaact tcgtcataaa ttgaaatatg tgcctcatga atatattgaa
      481 ttaattgaaa ttgccagaaa ttccactcag gatagaattc ttgaaatgaa ggtaatggaa
      541 ttttttatga aagtttatgg atatagaggt aaacatttgg gtggatcaag gaaaccggac
      601 ggagcaattt atactgtcgg atctcctatt gattacggtg tgatcgtgga tactaaagct
      661 tatagcggag gttataatct gccaattggc caagcagatg aaatgcaacg atatgtcaaa
      721 gaaaatcaaa cacgaaacaa acatatcaac cctaatgaat ggtggaaagt ctatccatct
      781 tctgtaacgg aatttaagtt tttatttgtg agtggtcact ttaaaggaaa ctacaaagct
      841 cagcttacac gattaaa
//
