LOCUS       sequence-389263-       13565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of hCOL4A5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13565)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-Blast_hCOL4A5
FEATURES             Location/Qualifiers
     source          1..13565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    524..649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1146..1379
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1564..1608
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1871..1986
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2041..3223
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2272..3214
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3171..3191
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        8830..9329
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8865..8888)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9262..9281
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      9343..9352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9364..9762
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9776..10364
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9829..9849)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10247..10258)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10571..10804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10933..10949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10933..10949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10946..10968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10957..10973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10981..10987,10988..11005,11006..11011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11026..11047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11164..11181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11335..11923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11415..11434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12094..12885,12886..12954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12717..12736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12955..13059)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13107..13241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13144..13163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13198..13217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(13409..13565,1..477)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
       61 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
      121 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
      181 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
      241 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
      301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      481 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      541 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      601 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      661 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      721 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
      781 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
      841 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
      901 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
      961 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     1021 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1081 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1141 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1201 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1261 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1321 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1381 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1441 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1501 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1561 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1621 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1681 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1741 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1801 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1861 cggtatcgcc tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1921 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1981 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgatatc gaattcgtga
     2041 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2101 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2161 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2221 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2281 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2341 acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2401 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2461 ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2521 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2581 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2641 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2701 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2761 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2821 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2881 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2941 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     3001 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     3061 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     3121 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     3181 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacctgca ggatttaaat
     3241 tctagagcat gaaactgcgt ggagtcagcc tggctgccgg cttgttctta ctggccctga
     3301 gtctttgggg gcagcctgca gaggctgcgg cttgctatgg gtgttctcca ggatcaaagt
     3361 gtgactgcag tggcataaaa ggggaaaagg gagagagagg gtttccaggt ttggaaggac
     3421 acccaggatt gcctggattt ccaggtccag aagggcctcc ggggcctcgg ggacaaaagg
     3481 gtgatgatgg aattccaggg ccaccaggac caaaaggaat cagaggtcct cctggacttc
     3541 ctggatttcc agggacacca ggtcttcctg gaatgccagg ccacgatggg gccccaggac
     3601 ctcaaggtat tcccggatgc aatggaacca agggagaacg tggatttcca ggcagtcccg
     3661 gttttcctgg tttacagggt cctccaggac cccctgggat cccaggtatg aagggtgaac
     3721 caggtagtat aattatgtca tcactgccag gaccaaaggg taatccagga tatccaggtc
     3781 ctcctggaat acaaggccta cctggtccca ctggtatacc agggccaatt ggtcccccag
     3841 gaccaccagg tttgatgggc cctcctggtc caccaggact tccaggacct aaggggaata
     3901 tgggcttaaa tttccaggga cccaaaggtg aaaaaggtga gcaaggtctt cagggcccac
     3961 ctgggccacc tgggcagatc agtgaacaga aaagaccaat tgatgtagag tttcagaaag
     4021 gagatcaggg acttcctggt gaccgagggc ctcctggacc tccagggata cgtggtcctc
     4081 caggtccccc aggtggtgag aaaggtgaga agggtgagca aggagagcca ggcaaaagag
     4141 gtaaaccagg caaagatgga gaaaatggcc aaccaggaat tcctggtttg cctggtgatc
     4201 ctggttaccc tggtgaaccc ggaagggatg gtgaaaaggg ccaaaaaggt gacactggcc
     4261 cacctggacc tcctggactt gtaattccta gacctgggac tggtataact ataggagaaa
     4321 aaggaaacat tgggttgcct gggttgcctg gagaaaaagg agagcgagga tttcctggaa
     4381 tacagggtcc acctggcctt cctggacctc caggggctgc agttatgggt cctcctggcc
     4441 ctcctggatt tcctggagaa aggggtcaga aaggtgatga aggaccacct ggaatttcca
     4501 ttcctggacc tcctggactt gacggacagc ctggggctcc tgggcttcca gggcctcctg
     4561 gccctgctgg ccctcacatt cctcctagtg atgagatatg tgaaccaggc cctccaggcc
     4621 ccccaggatc tccaggtgat aaaggactcc aaggagaaca aggagtgaaa ggtgacaaag
     4681 gtgacacttg cttcaactgc attggaactg gtatttcagg gcctccaggt caacctggtt
     4741 tgccaggtct cccaggtcct ccaggatctc ttggtttccc tggacagaaa ggggaaaaag
     4801 gacaagctgg tgcaactggt cccaaaggat taccaggcat tccaggagct ccaggtgctc
     4861 caggctttcc tggatctaaa ggtgaacctg gtgatatcct cacttttcca ggaatgaagg
     4921 gtgacaaagg agagttgggt tcccctggag ctccagggct tcctggttta cctggcactc
     4981 ctggacagga tggattgcca gggcttcctg gcccgaaagg agagcctggt ggaattactt
     5041 ttaagggtga aagaggtccc cctgggaacc caggtttacc aggcctccca gggaatatag
     5101 ggcctatggg tccccctggt ttcggccctc caggcccagt aggtgaaaaa ggcatacaag
     5161 gtgtggcagg aaatccaggc cagccaggaa taccaggtcc taaaggggat ccaggtcaga
     5221 ctataaccca gccggggaag cctggcttgc ctggtaaccc aggcagagat ggtgatgtag
     5281 gtcttccagg tgaccctgga cttccagggc aaccaggctt gccagggata cctggtagca
     5341 aaggagaacc aggtatccct ggaattgggc ttcctggacc acctggtccc aaaggctttc
     5401 ctggaattcc aggacctcca ggagcacctg ggacacctgg aagaattggt ctagaaggcc
     5461 ctcctgggcc acccggcttt ccaggaccaa agggtgaacc aggatttgca ttacctgggc
     5521 cacctgggcc accaggactt ccaggtttca aaggagcact tggtccaaaa ggtgatcgtg
     5581 gtttcccagg acctccgggt cctccaggac gcactggctt agatgggctc cctggaccaa
     5641 aaggtgatgt tggaccaaat ggacaacctg gaccaatggg acctcctggg ctgccaggaa
     5701 taggtgttca gggaccacca ggaccaccag ggattcctgg gccaataggt caacctggtt
     5761 tacatggaat accaggagag aagggggatc caggacctcc tggacttgat gttccaggac
     5821 ccccaggtga aagaggcagt ccagggatcc ccggagcacc tggtcctata ggacctccag
     5881 gatcaccagg gcttccagga aaagcaggtg cctctggatt tccaggtacc aaaggtgaaa
     5941 tgggtatgat gggacctcca ggcccaccag gacctttggg aattcctggc aggagtggtg
     6001 tacctggtct taaaggtgat gatggcttgc agggtcagcc aggacttcct ggccctacag
     6061 gagaaaaagg tagtaaagga gagcctggcc ttccaggccc tcctggacca atggatccaa
     6121 atcttctggg ctcaaaagga gagaaggggg aacctggctt accaggtata cctggagttt
     6181 cagggccaaa aggttatcag ggtttgcctg gagacccagg gcaacctgga ctgagtggac
     6241 aacctggatt accaggacca ccaggtccca aaggtaaccc tggtctccct ggacagccag
     6301 gtcttatagg acctcctgga cttaaaggaa ccatcggtga tatgggtttt ccagggcctc
     6361 agggtgtgga agggcctcct ggaccttctg gagttcctgg acaacctggc tccccaggat
     6421 tacctggaca gaaaggcgac aaaggtgatc ctggtatttc aagcattggt cttccaggtc
     6481 ttcctggtcc aaagggtgag cctggtctgc ctggataccc agggaaccct ggtatcaaag
     6541 gttctgtggg agatcctggt ttgcccggat taccaggaac ccctggagca aaaggacaac
     6601 caggccttcc tggattccca ggaaccccag gccctcctgg accaaaaggt attagtggcc
     6661 ctcctgggaa ccccggcctt ccaggagaac ctggtcctgt aggtggtgga ggtcatcctg
     6721 ggcaaccagg gcctccaggc gaaaaaggca aacccggtca agatggtatt cctggaccag
     6781 ctggacagaa gggtgaacca ggtcaaccag gctttggaaa cccaggaccg cctggacttc
     6841 caggactttc tggccaaaag ggtgatggag gattacctgg gattccagga aatcctggcc
     6901 ttccaggtcc aaagggcgaa ccaggctttc acggtttccc tggtgtgcag ggtcccccag
     6961 gccctcctgg ttctccgggt ccagctctgg aaggacctaa aggcaaccct gggccccaag
     7021 gtcctcctgg gagaccaggt cctacaggtt ttcaaggtct accaggtcca gaaggtcctc
     7081 caggtctccc tggaaatgga ggtattaaag gagagaaggg aaatccaggc caacctgggc
     7141 tacctggctt gcctggtttg aaaggagatc aaggaccacc aggactccag ggtaatcctg
     7201 gccggccggg tctcaatgga atgaaaggag atcctggtct ccctggtgtt ccaggattcc
     7261 caggcatgaa aggacccagt ggagtacctg gatcagctgg ccctgagggg gaaccgggac
     7321 ttattggtcc tccaggtcct cctggattac ctggtccttc aggacagagt atcataatta
     7381 aaggagatgc tggtcctcca ggaatccctg gccagcctgg gctaaagggt ctaccaggac
     7441 cccaaggacc tcaaggctta ccaggtccaa ctggccctcc aggagatcct ggacgcaatg
     7501 gactccctgg ctttgatggt gcaggagggc gcaaaggaga cccaggtctg ccaggacagc
     7561 caggtacccg tggtttggat ggtccccctg gtccagatgg attgcaaggt cccccaggtc
     7621 cccctggaac ctcctctgtt gcacatggat ttcttattac acgccacagc cagacaacgg
     7681 atgcaccaca atgcccacag ggaacacttc aggtctatga aggcttttct ctcctgtatg
     7741 tacaaggaaa taaaagagcc cacggtcaag acttggggac ggctggcagc tgccttcgtc
     7801 gctttagtac catgcctttc atgttctgca acatcaataa tgtttgcaac tttgcttcaa
     7861 gaaatgacta ttcttactgg ctctctaccc cagagcccat gccaatgagc atgcaacccc
     7921 taaagggcca gagcatccag ccattcatta gtcgatgtgc agtatgtgaa gctccagctg
     7981 tggtgatcgc agttcacagt cagacgatcc agattcccca ttgtcctcag ggatgggatt
     8041 ctctgtggat tggttattcc ttcatgatgc atacaagtgc aggggcagaa ggctcaggtc
     8101 aagccctagc ctcccctggt tcctgcttgg aagagtttcg ttcagctccc ttcatcgaat
     8161 gtcatgggag gggtacctgt aactactatg ccaactccta cagcttttgg ctggcaactg
     8221 tagatgtgtc agacatgttc agtaaacctc agtcagaaac gctgaaagca ggagacttga
     8281 ggacacgaat tagccgatgt caagtgtgca tgaagaggac ataatcgagc ggtgtcttca
     8341 cactcgaaga tttcgttggg gactgggaac agacagccgc ctacaacctg gaccaagtcc
     8401 ttgaacaggg aggtgtgtcc agtttgctgc agaatctcgc cgtgtccgta actccgatcc
     8461 aaaggattgt ccggagcggt gaaaatgccc tgaagatcga catccatgtc atcatcccgt
     8521 atgaaggtct gagcgccgac caaatggccc agatcgaaga ggtgtttaag gtggtgtacc
     8581 ctgtggatga tcatcacttt aaggtgatcc tgccctatgg cacactggta atcgacgggg
     8641 ttacgccgaa catgctgaac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg
     8701 gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc
     8761 tgatcacccc cgacggctcc atgctgttcc gagtaaccat caacagctaa ggccgcgacc
     8821 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     8881 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     8941 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     9001 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     9061 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     9121 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     9181 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     9241 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     9301 tctcctcttc ctcatctccg ggcctttcga cctgcagtta acgccgccat ggatagatcc
     9361 ggaatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     9421 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     9481 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     9541 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     9601 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     9661 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     9721 attcgtgaat tgctgccctc tggttatgtg tgggagggct aaacgcgtct ggaacaatca
     9781 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     9841 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     9901 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     9961 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    10021 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    10081 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    10141 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
    10201 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    10261 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    10321 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
    10381 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
    10441 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
    10501 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    10561 aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
    10621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10801 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    10861 tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt
    10921 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10981 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11041 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11881 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11941 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12001 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12061 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12121 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12181 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12241 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12301 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12361 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12421 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12481 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12541 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12601 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12661 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12721 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12781 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12841 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12901 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12961 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13021 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13081 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
    13141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13201 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    13261 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    13321 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    13381 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    13441 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    13501 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    13561 gttga
//
