LOCUS       sequence-38927-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Lindsey-Boltz LA, Sancar A
  TITLE     Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase 
            II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates 
            Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation
            of Checkpoint Kinase 1 (Chk1).
  JOURNAL   J Biol Chem. 2011 Jun 3. 286(22):19229-36.
  PUBMED    21502314
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jun 3. 286(22):19229-36."
COMMENT     Sequence Label: pcDNA3-LacR-TopBP1
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             305..325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             338..361
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
       61 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
      121 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
      181 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      301 ccgacccaag aagaagagga aggtgggatc caccatggat tacaaagacg atgacgacaa
      361 gtccagaaat gacaaagaac cgttttttgt gaagttttta aagtcttcag acaattccaa
      421 atgttttttt aaagctctcg agtccataaa agaattccaa tcagaagaat atcttcagat
      481 tattacagaa gaagaggcat tgaagataaa ggagaatgat agatcacttt atatctgtga
      541 cccttttagt ggcgttgtct ttgatcacct caaaaagctt ggctgcagaa ttgttggtcc
      601 tcaagtagtc atattttgta tgcaccacca gcgatgtgtc ccaagagccg aacatccagt
      661 ttataatatg gttatgtctg atgtaaccat atcttgtaca agtctggaaa aagaaaaaag
      721 ggaagaagtt cataaatatg tacaaatgat gggcggacga gtatacagag accttaatgt
      781 atcagtaact caccttattg caggagaagt tggtagcaaa aaatatttag ttgctgcaaa
      841 cctgaa
//
