LOCUS       sequence-389274-        8940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding tFucci(CA)5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: tFucci(CA)5pPBbsr2
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2751..2760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3804..3860
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4602..4931
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    5004..5553
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5148..5165)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5375..5394
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6024..6041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6030..6254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6301..6317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6325..6341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6349..6355,6356..6373,6374..6379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6394..6415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6649..6711
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6752..6772)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6752..6770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6791..6807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6815..6831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6839..6845,6846..6863,6864..6869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6884..6905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7022..7039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7193..7781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7273..7292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7952..8743,8744..8812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8575..8594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8813..8917)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggt cgacggatcc gccaccatgg
     2761 agaacgtcag gagaaagaca ggcattcaga cggaaatgaa aacgaagctc cacatggatg
     2821 gtatggtgaa tggacacagc tttgagatca aaggggaggg gaagggaagc ccgtacgagg
     2881 gggtgcagac aatgaagctg aaggttacta agggcgcccc acttcccttc tctatagaca
     2941 ttcttttgcc tcagtgcatg tatggcagca aacctttcat taaatatcct gagaacatcc
     3001 ccgattattt gaaactctca tttccagagg gtatcacttg ggagaggaca atgacattcg
     3061 aggacggcgc agtatgcgat gtatctaatg attccagact ggtcggcaat tgctttatct
     3121 acactgtgaa gtttcaggga gtcaattttc cacttgatgg ccccgttatg caaaagaaga
     3181 cccgaggctg ggaaccctcc actgaagtgc tgtacgaatg tgatgggtgg atgcgcgggt
     3241 tggtggacat agctctgaaa ctggaaaacg gtggacatta tatgtgtaat tttaagacca
     3301 cctacaaatc caagaaaggt cttgaggtgc caccttacca tttcgtggat cataaactcg
     3361 acctgctcag tcataacacc gacggagcca cctttgagga gttcgaacaa ggtgaaatcg
     3421 ctcacgcgca cttgagtaaa ctggccggat atccatcaca ctggcggccg ctcgagatgg
     3481 agcagcgccg cgtcaccgac ttcttcgcgc gccgccgccc cgggcccccc cgcatcgcgc
     3541 cgcccaagct ggcctgccgc acccccagcc ccgccaggcc cgcactccgc gccccggcct
     3601 ccgctaccag tggcagccgc aagcgcgccc gcccgcccgc cgcccccgga cgcgaccagg
     3661 ccaggccacc ggcccgcgcg gccgcgcggc tgtcggtgga cgaggtttcc agccccagta
     3721 cccccgaggc cccagacatc ccagcctgcc cttctccggg ccagaagata aagaaaaccg
     3781 gtcgcgccaa gcgcggcagc ggcgccacca acttcagcct gctgaagcag gccggcgacg
     3841 tggaggagaa ccccggcccc cgcgaattca tgagtaaact ccagaaaggt gtggagaaag
     3901 agatgaagat taagctcact atgaactgca ccgtgaacga acggaacttt gtgatcacag
     3961 ggcagggggc tggggaacct tatgatggaa ctcagacact ttacctgaca gtggagggcg
     4021 gaaagacact gaacttcagc ttcgatgtac tgaccccagc ctttcagtac ggcaataggg
     4081 cgtttaccaa gtaccccggt aacataccag acttcttcaa acagaccttc agtggcgggg
     4141 gatacacatg gaaaagaaca atgacctatg aagacggcgg cgtctcaacc gttgagtccg
     4201 atatcagcgt tcaaggggac tgcttccatt ataaaataca attcaacgga aagttccccc
     4261 cccacggccc agtcatgcaa aaggaaacag tcaaatggga accttccacc gaggtgatgt
     4321 acaaggacga taaaaacgat ggagtgctga agggcgacgt gaatatggcc ctgctgttga
     4381 aggatggtgg tcacctcaga gtcgatttca acacttctta tattcccaaa aataaggtgg
     4441 agaagatgcc agattaccac ttcgtggacc accggatcga gatcctggag aagcccgaag
     4501 gacgccctgt caagctctac gcaggggctg tggccaggta cagcctgctg cctgaaaaga
     4561 acctgaataa gggatatcca tcacactggc ggccgctcga gatgaatccc agtatgaagc
     4621 agaaacaaga agaaatcaaa gagaatataa agaatagttc tgtcccaaga agaactctga
     4681 agatgattca gccttctgca tctggatctc ttgttggaag agaaaatgag ctgtccgcag
     4741 gcttgtccaa aaggaaacat cggaatgacc acttaacatc tacaacttcc agccctgggg
     4801 ttattgtccc agaatctagt gaaaataaaa atcttggagg agtcacccag gagtcatttg
     4861 atcttatgat taaagaaaat ccatcctctc agtattggaa ggaagtggca gaaaaacgga
     4921 gaaaggcgct gtaatctaga gttaactaac ttaagctagc aacggtttcc ctctagcggg
     4981 atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5041 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5101 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5161 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5221 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5281 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5341 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5401 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     5461 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     5521 ggacgtggtt ttcctttgaa aaacacgata ataccatggt catgaaaaca tttaacattt
     5581 ctcaacaaga tctagaatta gtagaagtag cgacagagaa gattacaatg ctttatgagg
     5641 ataataaaca tcatgtggga gcggcaattc gtacgaaaac aggagaaatc atttcggcag
     5701 tacatattga agcgtatata ggacgagtaa ctgtttgtgc agaagccatt gcgattggta
     5761 gtgcagtttc gaatggacaa aaggattttg acacgattgt agctgttaga cacccttatt
     5821 ctgacgaagt agatagaagt attcgagtgg taagtccttg tggtatgtgt agggagttga
     5881 tttcagacta tgcaccagat tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa
     5941 ctacgattga agaactcatt ccactcaaat atacccgaaa ttaaaagtcg agatgcatcg
     6001 atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6061 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6121 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6181 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6241 ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg
     6301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcttaaaagt
     6481 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
     6541 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
     6601 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
     6661 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaatctag
     6721 tatacgcgtt taattaatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     6781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6841 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6901 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6961 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7621 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
