LOCUS       sequence-389276-       10966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB1 mutant tagged with mScarlet..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pKUNOC-ErbB1(M721)-mScarlet
FEATURES             Location/Qualifiers
     source          1..10966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1681..1914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2099..2143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2635..2938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2939..3142
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(3194..3265,3266..5128,5129..5197,5198..6823)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIMELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             6824..7519
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6957..6975)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7225..7244
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7572..8160
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7625..7645)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8043..8054)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8679..8859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8885..8902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8891..9115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9211..9799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9291..9310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9970..10761,10762..10830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10831..10935)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaaa ttcactttga attaattcga
     2581 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2641 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2701 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2761 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2821 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2881 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2941 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3001 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3061 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3121 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
     3181 tcgagaattc accatgcgac cctccgggac ggccggggca gcgctcctgg cgctgctggc
     3241 tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag gcacgagtaa
     3301 caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga ggatgttcaa
     3361 taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga attatgatct
     3421 ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc tcaacacagt
     3481 ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact acgaaaattc
     3541 ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga aggagctgcc
     3601 catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca accctgccct
     3661 gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc tcagcaacat
     3721 gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa gctgtcccaa
     3781 tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa tcatctgtgc
     3841 ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc acaaccagtg
     3901 tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca aattccgaga
     3961 cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca ccacgtacca
     4021 gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga agaagtgtcc
     4081 ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg ccgacagcta
     4141 tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt gccgcaaagt
     4201 gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg ctacgaatat
     4261 taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc cggtggcatt
     4321 taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg atattctgaa
     4381 aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa acaggacgga
     4441 cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac atggtcagtt
     4501 ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc tcaaggagat
     4561 aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa atacaataaa
     4621 ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca acagaggtga
     4681 aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg agggctgctg
     4741 gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca gggaatgcgt
     4801 ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact ctgagtgcat
     4861 acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag gacggggacc
     4921 agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca agacctgccc
     4981 ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg ccggccatgt
     5041 gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc ttgaaggctg
     5101 tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg ccctcctctt
     5161 gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca tcgttcggaa
     5221 gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg
     5281 agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa
     5341 agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga
     5401 gaaagttaaa attcccgtcg cgatcatgga attaagagaa gcaacatctc cgaaagccaa
     5461 caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg
     5521 cctgctgggc atctgcctca cctccaccgt gcagctcatc acgcagctca tgcccttcgg
     5581 ctgcctcctg gactatgtcc gggaacacaa agacaatatt ggctcccagt acctgctcaa
     5641 ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag gaccgtcgct tggtgcaccg
     5701 cgacctggca gccaggaacg tactggtgaa aacaccgcag catgtcaaga tcacagattt
     5761 tgggctggcc aaactgctgg gtgcggaaga gaaagaatac catgcagaag gaggcaaagt
     5821 gcctatcaag tggatggcat tggaatcaat tttacacaga atctataccc accagagtga
     5881 tgtctggagc tacggggtga ccgtttggga gttgatgacc tttggatcca agccatatga
     5941 cggaatccct gccagcgaga tctcctccat cctggagaaa ggagaacgcc tccctcagcc
     6001 acccatatgt accatcgatg tctacatgat catggtcaag tgctggatga tagacgcaga
     6061 tagtcgccca aagttccgtg agttgatcat cgaattctcc aaaatggccc gagaccccca
     6121 gcgctacctt gtcattcagg gggatgaaag aatgcatttg ccaagtccta cagattccaa
     6181 cttctaccgt gccctgatgg atgaagaaga catggacgac gtggtggatg ccgacgagta
     6241 cctcatccca cagcagggct tcttcagcag cccctccacg tcacggactc ccctcctgag
     6301 ctctctgagt gcaaccagca acaattccac cgtggcttgc attgatagaa atgggctgca
     6361 aagctgtccc atcaaggaag acagcttctt gcagcgatac agctcagacc ccacaggcgc
     6421 cttgactgag gacagcatag acgacacctt cctcccagtg cctgaataca taaaccagtc
     6481 cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc tatcacaatc agcctctgaa
     6541 ccccgcgccc agcagagacc cacactacca ggacccccac agcactgcag tgggcaaccc
     6601 cgagtatctc aacactgtcc agcccacctg tgtcaacagc acattcgaca gccctgccca
     6661 ctgggcccag aaaggcagcc accaaattag cctggacaac cctgactacc agcaggactt
     6721 ctttcccaag gaagccaagc caaatggcat ctttaagggc tccacagctg aaaatgcaga
     6781 atacctaagg gtcgcgccac aaagcagtga atttattgga gcaatggtga gcaagggcga
     6841 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     6901 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     6961 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     7021 catgtacggc tccagggcct tcaccaagca ccccgccgac atccccgact actataagca
     7081 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     7141 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     7201 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     7261 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     7321 gcgcctgaag gacggaggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     7381 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     7441 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     7501 catggacgag ctgtacaagt aagcggccgc ggatcctcta gagttaacat cgagggatca
     7561 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7981 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8161 atcgataccg tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8221 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8281 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8401 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     8461 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     8521 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     8581 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     8641 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     8701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     8881 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8941 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9001 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9061 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9121 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggatat
     9181 cccgggacgt caggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
