LOCUS       sequence-389279-       10510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBbleo-ErbB2
FEATURES             Location/Qualifiers
     source          1..10510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6628..7177
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6772..6789)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6999..7018
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7180..7554
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(7594..7611)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7600..7824
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7871..7887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7895..7911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7919..7925,7926..7943,7944..7949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7964..7985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8219..8281
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8322..8342)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8322..8340)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8361..8377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8385..8401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8409..8415,8416..8433,8434..8439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8454..8475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8592..8609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8763..9351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8843..8862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9522..10313,10314..10382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10145..10164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10383..10487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagacca
     2761 tggagctggc ggccttgtgc cgctgggggc tcctcctcgc cctcttgccc cccggagccg
     2821 cgagcaccca agtgtgcacc ggcacagaca tgaagctgcg gctccctgcc agtcccgaga
     2881 cccacctgga catgctccgc cacctctacc agggctgcca ggtggtgcag ggaaacctgg
     2941 aactcaccta cctgcccacc aatgccagcc tgtccttcct gcaggatatc caggaggtgc
     3001 agggctacgt gctcatcgct cacaaccaag tgaggcaggt cccactgcag aggctgcgga
     3061 ttgtgcgagg cacccagctc tttgaggaca actatgccct ggccgtgcta gacaatggag
     3121 acccgctgaa caataccacc cctgtcacag gggcctcccc aggaggcctg cgggagctgc
     3181 agcttcgaag cctcacagag atcttgaaag gaggggtctt gatccagcgg aacccccagc
     3241 tctgctacca ggacacgatt ttgtggaagg acatcttcca caagaacaac cagctggctc
     3301 tcacactgat agacaccaac cgctctcggg cctgccaccc ctgttctccg atgtgtaagg
     3361 gctcccgctg ctggggagag agttctgagg attgtcagag cctgacgcgc actgtctgtg
     3421 ccggtggctg tgcccgctgc aaggggccac tgcccactga ctgctgccat gagcagtgtg
     3481 ctgccggctg cacgggcccc aagcactctg actgcctggc ctgcctccac ttcaaccaca
     3541 gtggcatctg tgagctgcac tgcccagccc tggtcaccta caacacagac acgtttgagt
     3601 ccatgcccaa tcccgagggc cggtatacat tcggcgccag ctgtgtgact gcctgtccct
     3661 acaactacct ttctacggac gtgggatcct gcaccctcgt ctgccccctg cacaaccaag
     3721 aggtgacagc agaggatgga acacagcggt gtgagaagtg cagcaagccc tgtgcccgag
     3781 tgtgctatgg tctgggcatg gagcacttgc gagaggtgag ggcagttacc agtgccaata
     3841 tccaggagtt tgctggctgc aagaagatct ttgggagcct ggcatttctg ccggagagct
     3901 ttgatgggga cccagcctcc aacactgccc cgctccagcc agagcagctc caagtgtttg
     3961 agactctgga agagatcaca ggttacctat acatctcagc atggccggac agcctgcctg
     4021 acctcagcgt cttccagaac ctgcaagtaa tccggggacg aattctgcac aatggcgcct
     4081 actcgctgac cctgcaaggg ctgggcatca gctggctggg gctgcgctca ctgagggaac
     4141 tgggcagtgg actggccctc atccaccata acacccacct ctgcttcgtg cacacggtgc
     4201 cctgggacca gctctttcgg aacccgcacc aagctctgct ccacactgcc aaccggccag
     4261 aggacgagtg tgtgggcgag ggcctggcct gccaccagct gtgcgcccga gggcactgct
     4321 ggggtccagg gcccacccag tgtgtcaact gcagccagtt ccttcggggc caggagtgcg
     4381 tggaggaatg ccgagtactg caggggctcc ccagggagta tgtgaatgcc aggcactgtt
     4441 tgccgtgcca ccctgagtgt cagccccaga atggctcagt gacctgtttt ggaccggagg
     4501 ctgaccagtg tgtggcctgt gcccactata aggaccctcc cttctgcgtg gcccgctgcc
     4561 ccagcggtgt gaaacctgac ctctcctaca tgcccatctg gaagtttcca gatgaggagg
     4621 gcgcatgcca gccttgcccc atcaactgca cccactcctg tgtggacctg gatgacaagg
     4681 gctgccccgc cgagcagaga gccagccctc tgacgtccat catctctgcg gtggttggca
     4741 ttctgctggt cgtggtcttg ggggtggtct ttgggatcct catcaagcga cggcagcaga
     4801 agatccggaa gtacacgatg cggagactgc tgcaggaaac ggagctggtg gagccgctga
     4861 cacctagcgg agcgatgccc aaccaggcgc agatgcggat cctgaaagag acggagctga
     4921 ggaaggtgaa ggtgcttgga tctggcgctt ttggcacagt ctacaagggc atctggatcc
     4981 ctgatgggga gaatgtgaaa attccagtgg ccatcaaagt gttgagggaa aacacatccc
     5041 ccaaagccaa caaagaaatc ttagacgaag catacgtgat ggctggtgtg ggctccccat
     5101 atgtctcccg ccttctgggc atctgcctga catccacggt gcagctggtg acacagctta
     5161 tgccctatgg ctgcctctta gaccatgtcc gggaaaaccg cggacgcctg ggctcccagg
     5221 acctgctgaa ctggtgtatg cagattgcca aggggatgag ctacctggag gatgtgcggc
     5281 tcgtacacag ggacttggcc gctcggaacg tgctggtcaa gagtcccaac catgtcaaaa
     5341 ttacagactt cgggctggct cggctgctgg acattgacga gacagagtac catgcagatg
     5401 ggggcaaggt gcccatcaag tggatggcgc tggagtccat tctccgccgg cggttcaccc
     5461 accagagtga tgtgtggagt tatggtgtga ctgtgtggga gctgatgact tttggggcca
     5521 aaccttacga tgggatccca gcccgggaga tccctgacct gctggaaaag ggggagcggc
     5581 tgccccagcc ccccatctgc accattgatg tctacatgat catggtcaaa tgttggatga
     5641 ttgactctga atgtcggcca agattccggg agttggtgtc tgaattctcc cgcatggcca
     5701 gggaccccca gcgctttgtg gtcatccaga atgaggactt gggcccagcc agtcccttgg
     5761 acagcacctt ctaccgctca ctgctggagg acgatgacat gggggacctg gtggatgctg
     5821 aggagtatct ggtaccccag cagggcttct tctgtccaga ccctgccccg ggcgctgggg
     5881 gcatggtcca ccacaggcac cgcagctcat ctaccaggag tggcggtggg gacctgacac
     5941 tagggctgga gccctctgaa gaggaggccc ccaggtctcc actggcaccc tccgaagggg
     6001 ctggctccga tgtatttgat ggtgacctgg gaatgggggc agccaagggg ctgcaaagcc
     6061 tccccacaca tgaccccagc cctctacagc ggtacagtga ggaccccaca gtacccctgc
     6121 cctctgagac tgatggctac gttgcccccc tgacctgcag cccccagcct gaatatgtga
     6181 accagccaga tgttcggccc cagccccctt cgccccgaga gggccctctg cctgctgccc
     6241 gacctgctgg tgccactctg gaaaggccca agactctctc cccagggaag aatggggtcg
     6301 tcaaagacgt ttttgccttt gggggtgccg tggagaaccc cgagtacttg acaccccagg
     6361 gaggagctgc ccctcagccc caccctcctc ctgccttcag cccagccttc gacaacctct
     6421 attactggga ccaggaccca ccagagcggg gggctccacc cagcaccttc aaagggacac
     6481 ctacggcaga gaacccagag tacctgggtc tggacgtgcc agtgtgagcg gccgctctag
     6541 agtcgacggg ccgcggtaac aattgttaac taacttaagc tagcaacggt ttccctctag
     6601 cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     6661 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     6721 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     6781 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     6841 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     6901 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6961 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     7021 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     7081 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     7141 acggggacgt ggttttcctt tgaaaaacac gataatacca tggccaagtt gaccagtgcc
     7201 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     7261 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     7321 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     7381 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     7441 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     7501 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaaagtcg
     7561 agatgcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     7621 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7681 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7741 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7801 ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg
     7861 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     8041 tcttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
     8101 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
     8161 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
     8221 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
     8281 gttaatctag tatacgcgtt taattaatcc agcttttgtt ccctttagtg agggttaatt
     8341 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8401 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8461 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8521 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10501 aaagtgccac
//
