LOCUS       sequence-389281-       10741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible LDLR coding sequence expression construct cloned into 
            a lentiviral backbone containing a blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Hamilton MC, Fife JD, Akinci E, Yu T, Khowpinitchai B, Cha M, Barkal
            S, Thi TT, Yeo GHT, Barroso JPR, Francoeur MJ, Velimirovic M, 
            Gifford DK, Lettre G, Yu H, Cassa CA, Sherwood RI
  TITLE     Systematic elucidation of genetic mechanisms underlying cholesterol 
            uptake.
  JOURNAL   bioRxiv. 2023 Jan 10:2023.01.09.500804. doi: 
            10.1101/2023.01.09.500804. Preprint.
  PUBMED    36711952
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jan 10:2023.01.09.500804. doi: 10.1101/2023.01.09.500804. 
            Preprint."
COMMENT     Sequence Label: lentiTet-LDLR-mCherry BlastR
FEATURES             Location/Qualifiers
     source          1..10741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2978..2989)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3163..3870
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3308..3326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3576..3595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3884..4394
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4313..4331
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4404..4799
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4800..4853
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4854..5471,5472..5477,5478..5513,5514..5516,5517..5552,
                     5553..5555,5556..5591,5592..5600)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5640..5660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6058..6069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6274..6507
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6579..6700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6616..6635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6670..6689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6740..6875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6875..6946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6947..7015,7016..7807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7165..7184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7978..8566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8467..8486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8720..8737
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8854..8875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8890..8895,8896..8913,8914..8920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8928..8944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8933..8955
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8952..8968
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8952..8968
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8987..9007
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8989..9007
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9035..9261
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9262..9442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9489..9614
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10107..10340
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10525..10569
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gccaccatgg atatcggatc catggggccc tggggctgga aattgcgctg gaccgtcgcc
      601 ttgctcctcg ccgcggcggg gactgcagtg ggcgacagat gtgaaagaaa cgagttccag
      661 tgccaagacg ggaaatgcat ctcctacaag tgggtctgcg atggcagcgc tgagtgccag
      721 gatggctctg atgagtccca ggagacgtgc ttgtctgtca cctgcaaatc cggggacttc
      781 agctgtgggg gccgtgtcaa ccgctgcatt cctcagttct ggaggtgcga tggccaagtg
      841 gactgcgaca acggctcaga cgagcaaggc tgtcccccca agacgtgctc ccaggacgag
      901 tttcgctgcc acgatgggaa gtgcatctct cggcagttcg tctgtgactc agaccgggac
      961 tgcttggacg gctcagacga ggcctcctgc ccggtgctca cctgtggtcc cgccagcttc
     1021 cagtgcaaca gctccacctg catcccccag ctgtgggcct gcgacaacga ccccgactgc
     1081 gaagatggct cggatgagtg gccgcagcgc tgtaggggtc tttacgtgtt ccaaggggac
     1141 agtagcccct gctcggcctt cgagttccac tgcctaagtg gcgagtgcat ccactccagc
     1201 tggcgctgtg atggtggccc cgactgcaag gacaaatctg acgaggaaaa ctgcgctgtg
     1261 gccacctgtc gccctgacga attccagtgc tctgatggaa actgcatcca tggcagccgg
     1321 cagtgtgacc gggaatatga ctgcaaggac atgagcgatg aagttggctg cgttaatgtg
     1381 acactctgcg agggacccaa caagttcaag tgtcacagcg gcgaatgcat caccctggac
     1441 aaagtctgca acatggctag agactgccgg gactggtcag atgaacccat caaagagtgc
     1501 gggaccaacg aatgcttgga caacaacggc ggctgttccc acgtctgcaa tgaccttaag
     1561 atcggctacg agtgcctgtg ccccgacggc ttccagctgg tggcccagcg aagatgcgaa
     1621 gatatcgatg agtgtcagga tcccgacacc tgcagccagc tctgcgtgaa cctggagggt
     1681 ggctacaagt gccagtgtga ggaaggcttc cagctggacc cccacacgaa ggcctgcaag
     1741 gctgtgggct ccatcgccta cctcttcttc accaaccggc acgaggtcag gaagatgacg
     1801 ctggaccgga gcgagtacac cagcctcatc cccaacctga ggaacgtggt cgctctggac
     1861 acggaggtgg ccagcaatag aatctactgg tctgacctgt cccagagaat gatctgcagc
     1921 acccagcttg acagagccca cggcgtctct tcctatgaca ccgtcatcag cagggacatc
     1981 caggcccccg acgggctggc tgtggactgg atccacagca acatctactg gaccgactct
     2041 gtcctgggca ctgtctctgt tgcggatacc aagggcgtga agaggaaaac gttattcagg
     2101 gagaacggct ccaagccaag ggccatcgtg gtggatcctg ttcatggctt catgtactgg
     2161 actgactggg gaactcccgc caagatcaag aaagggggcc tgaatggtgt ggacatctac
     2221 tcgctggtga ctgaaaacat tcagtggccc aatggcatca ccctagatct cctcagtggc
     2281 cgcctctact gggttgactc caaacttcac tccatctcaa gcatcgatgt caatgggggc
     2341 aaccggaaga ccatcttgga ggatgaaaag aggctggccc accccttctc cttggccgtc
     2401 tttgaggaca aagtattttg gacagatatc atcaacgaag ccattttcag tgccaaccgc
     2461 ctcacaggtt ccgatgtcaa cttgttggct gaaaacctac tgtccccaga ggatatggtc
     2521 ctcttccaca acctcaccca gccaagagga gtgaactggt gtgagaggac caccctgagc
     2581 aatggcggct gccagtatct gtgcctccct gccccgcaga tcaaccccca ctcgcccaag
     2641 tttacctgcg cctgcccgga cggcatgctg ctggccaggg acatgaggag ctgcctcaca
     2701 gaggctgagg ctgcagtggc cacccaggag acatccaccg tcaggctaaa ggtcagctcc
     2761 acagccgtaa ggacacagca cacaaccacc cggcctgttc ccgacacctc ccggctgcct
     2821 ggggccaccc ctgggctcac cacggtggag atagtgacaa tgtctcacca agctctgggc
     2881 gacgttgctg gcagaggaaa tgagaagaag cccagtagcg tgagggctct gtccattgtc
     2941 ctccccatcg tgctcctcgt cttcctttgc ctgggggtct tccttctatg gaagaactgg
     3001 cggcttaaga acatcaacag catcaacttt gacaaccccg tctatcagaa gaccacagag
     3061 gatgaggtcc acatttgcca caaccaggac ggctacagct acccctcgag acagatggtc
     3121 agtctggagg atgacgtggc gaccggtgga ccaggtacgc gtatggtgag caagggcgag
     3181 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3241 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3301 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3361 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3421 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3481 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3541 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3601 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     3661 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3721 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     3781 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     3841 tccaccggcg gcatggacga gctgtacaag atcgtttaat taaggggttg gggttgcgcc
     3901 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     3961 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     4021 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     4081 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     4141 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     4201 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     4261 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     4321 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     4381 acctctctcc ccagcaattc accatggcca agcctttgtc tcaagaagaa tccaccctca
     4441 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4501 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     4561 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     4621 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     4681 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     4741 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg
     4801 aaggtagagg ttctctcctc acttgtggtg atgttgaaga aaaccctggt ccaatgtcta
     4861 gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     4921 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     4981 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     5041 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     5101 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     5161 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     5221 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     5281 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     5341 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     5401 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     5461 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     5521 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     5581 ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat tcttaactat
     5641 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5701 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5761 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5821 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5881 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5941 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6001 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6061 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6121 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtt
     6181 tcgcctcggc gtccggacta gaggtacctt taagaccaat gacttacaag gcagctgtag
     6241 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6301 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6361 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6421 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6481 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6541 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6601 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6661 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6721 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6841 attccagaag tagtgaggag gcttttttgg aggcctttca aatatgtatc cgctcatgag
     6901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6961 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7021 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7081 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7141 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7201 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7261 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7321 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7381 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7441 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7501 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7561 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7621 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7681 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7741 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7801 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8101 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8581 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8641 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8701 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8761 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8821 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8881 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8941 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9001 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     9061 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     9121 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     9181 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     9241 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     9301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9421 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9481 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9541 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9601 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9661 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9721 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9781 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9841 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9901 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9961 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    10021 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    10081 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    10141 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    10201 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    10261 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    10321 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    10381 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10441 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10501 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10561 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10621 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10681 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10741 c
//
