LOCUS       sequence-389321-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pol II expression of U6 Lsm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lipinski KA, Chi J, Chen X, Hoskins AA, Brow DA
  TITLE     Yeast U6 snRNA made by RNA polymerase II is less stable but 
            functional.
  JOURNAL   RNA. 2022 Dec;28(12):1606-1620. doi: 10.1261/rna.079328.122. Epub 
            2022 Oct 4.
  PUBMED    36195346
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079328.122"; journalName: "RNA"; date: "2022-12"; 
            volume: "28"; issue: "12"; pages: "1606-1620"
COMMENT     Sequence Label: RS314-U6(PolII-Lsm)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..43)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..44)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    201..318
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagatcat attacatggc
       61 attaccacca tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag
      121 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
      181 ctcattgcta tattgaagta cggattagaa gccgccgagc gggcgacagc cctccgacgg
      241 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
      301 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
      361 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa ttaacgaatc aaattaacaa
      421 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
      481 gcgatgattt ttgatctatt aacagatata aaaggaacag aatattagtt agcttcattg
      541 tccaattact attgttactt acgtttaatg cattttcttt tctttcactt ccttccttaa
      601 tactccgttc gcgaagtaac ccttcgtgga catttggtca atttgaaaca atacagagat
      661 gatcagcagt tcccctgcat aaggatgaac cgttttacaa agagatttat ttcgttttaa
      721 ttctctaatg tgagttcacg ttatagaagc tggaattgta agtaatacag aaatggcaac
      781 taaaatggta ggcatgagtg ttctccttct tgtgtgtctt tcttttcacc cgatacagta
      841 aactttgtat aggagagttg accaattacc tgacagtgcc
//
