LOCUS       sequence-389341-       10507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector containing GFP tagged PKCB1. Kinase inactive 
            mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W, Peng X, 
            Eastburn DJ, Ewald AJ, Werb Z, Mostov KE
  TITLE     A molecular switch for the orientation of epithelial cell 
            polarization.
  JOURNAL   Dev Cell. 2014 Oct 27;31(2):171-87. doi: 
            10.1016/j.devcel.2014.08.027. Epub 2014 Oct 9.
  PUBMED    25307480
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.08.027"; journalName: "Dev Cell"; date: 
            "2014-10-27- 27"; volume: "31"; issue: "2"; pages: "171-87"
COMMENT     Sequence Label: mVenus-PKCB1 K371R
FEATURES             Location/Qualifiers
     source          1..10507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2257..2266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2263..2265,2266..2268,2269..2979)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2308..2329)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2569..2588)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2916..2937
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5035..5608
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5202..5219)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5429..5448
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5647..6666
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5647..6666
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             7213..7638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7741..7763)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7899..7919)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7904..8233
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8084..8219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8255..8274
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8355..8372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8526..9114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8606..8625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9285..10076,10077..10145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9908..9927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10146..10250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10318..10336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10436..10507,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtcgcca ccatggtgag caagggcgag
     2281 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2341 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2401 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac cactctgggc
     2461 tacggcctac agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     2521 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2581 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2641 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2701 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     2761 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     2821 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     2881 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2941 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctatggct
     3001 gacccggctg cggggccgcc gccgagcgag ggcgaggaga gcaccgtgcg cttcgcccgc
     3061 aaaggcgccc tccggcagaa gaacgtgcat gaggtcaaga accacaaatt caccgcccgc
     3121 ttcttcaagc agcccacctt ctgcagccac tgcaccgact tcatctgggg cttcgggaag
     3181 cagggattcc agtgccaagt ttgctgcttt gtggtgcaca agcggtgcca tgaatttgtc
     3241 acattctcct gccctggcgc tgacaagggt ccagcctccg atgacccccg cagcaaacac
     3301 aagtttaaga tccacacgta ctccagcccc acgttttgtg accactgtgg gtcactgctg
     3361 tatggactca tccaccaggg gatgaaatgc gacacttgta tgatgaatgt gcacaagcgc
     3421 tgcgtgatga atgttcccag cctgtgtggc acggaccaca cggagcgccg cggccgcatc
     3481 tacatccagg cccacatcga cagggacgtc ctcattgtcc tcgtaagaga tgctaaaaac
     3541 cttgtaccta tggaccccaa tggcctgtca gatccctacg taaaactgaa actgattccc
     3601 gatcccaaaa gtgagagcaa acagaagacc aaaaccatca aatgctccct caaccctgag
     3661 tggaatgaga catttagatt tcagctgaaa gaatcggaca aagacagaag actgtcagta
     3721 gagatttggg attgggattt gaccagcagg aatgacttca tgggatcttt gtcctttggg
     3781 atttctgaac ttcagaaagc cagtgttgat ggctggttta agttactgag ccaggaggaa
     3841 ggcgagtact tcaatgtgcc tgtgccacca gaaggaagtg aggccaatga agaactgcgg
     3901 cagaaatttg agagggccaa gatcagtcag ggaaccaagg tcccggaaga aaagacgacc
     3961 aacactgtct ccaaatttga caacaatggc aacagagacc ggatgaaact gaccgatttt
     4021 aacttcctaa tggtgctggg gaaaggcagc tttggcaagg tcatgctttc agaacgaaaa
     4081 ggcacagatg agctctatgc tgtgagaatc ctgaagaagg acgttgtgat ccaagatgat
     4141 gacgtggagt gcactatggt ggagaagcgg gtgttggccc tgcctgggaa gccgcccttc
     4201 ctgacccagc tccactcctg cttccagacc atggaccgcc tgtactttgt gatggagtac
     4261 gtgaatgggg gcgacctcat gtatcacatc cagcaagtcg gccggttcaa ggagccccat
     4321 gctgtatttt acgctgcaga aattgccatc ggtctgttct tcttacagag taagggcatc
     4381 atttaccgtg acctaaaact tgacaacgtg atgctcgatt ctgagggaca catcaagatt
     4441 gccgattttg gcatgtgtaa ggaaaacatc tgggatgggg tgacaaccaa gacattctgt
     4501 ggcactccag actacatcgc ccccgagata attgcttatc agccctatgg gaagtccgtg
     4561 gattggtggg catttggagt cctgctgtat gaaatgttgg ctgggcaggc accctttgaa
     4621 ggggaggatg aagatgaact cttccaatcc atcatggaac acaacgtagc ctatcccaag
     4681 tctatgtcca aggaagctgt ggccatctgc aaagggctga tgaccaaaca cccaggcaaa
     4741 cgtctgggtt gtggacctga aggcgaacgt gatatcaaag agcatgcatt tttccggtat
     4801 attgattggg agaaacttga acgcaaagag atccagcccc cttataagcc aaaagctaga
     4861 gacaagagag acacctccaa cttcgacaaa gagttcacca gacagcctgt ggaactgacc
     4921 cccactgata aactcttcat catgaacttg gaccaaaatg aatttgctgg cttctcttat
     4981 actaacccag agtttgtcat taatgtgtag ggatccggaa ttccgccccc ccccccccyc
     5041 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     5101 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     5161 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     5221 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     5281 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     5341 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     5401 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     5461 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     5521 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     5581 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccgtaccaaa gatggataga
     5641 tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     5701 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     5761 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     5821 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     5881 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     5941 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6001 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6061 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6121 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6181 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6241 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6301 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6361 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6421 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6481 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6541 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     6601 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     6661 gaatagagta gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc
     6721 gcgcgagcct agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg
     6781 tccacagttc gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg
     6841 cccttctgga ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc
     6901 tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcta
     6961 tcgagggctg cagcgctgca gaggccgagt gcagaactgc tccaaaggga cctcaaggct
     7021 ttccgaggga cactaggctg actccatcga gccagtgtag agataagctt atcgattagt
     7081 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca
     7141 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca
     7201 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     7261 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     7321 aacagatgga acagggtcga ccctagagaa ccatcagatg tttccagggt gccccaagga
     7381 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     7441 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     7501 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     7561 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     7621 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     7681 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     7741 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7801 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     7861 ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg
     7921 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7981 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8041 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8101 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8161 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8221 aggcttttgc aaaaagctta ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8281 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8941 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9601 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9901 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10261 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10321 aggcgtatca cgaggccctt tcgtcttcaa gaattagctt ggccattgca tacgttgtat
    10381 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat
    10441 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    10501 atggagt
//
