LOCUS       sequence-389346-       10166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for the neuronal expression of 
            ChR2(H134R)-EYFP (Channelrhodopsin for the inducing neuronal 
            activity).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  TITLE     Tools for analysing neuronal cultures in vitro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-hSyn1-ChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        509..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             725..905
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    952..1077
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1570..1803
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1988..2032
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2329..2446
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2501..2948
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3003..3012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3009..3935
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      3945..3954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3951..3953,3954..3956,3957..4670)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3996..4017)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4257..4276)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4604..4625
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4752..5340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4805..4825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5223..5234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5343..5359)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5344..5360)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5861..6041
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6067..6084)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6073..6297
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6343..6771
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6430..6449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6640..6661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6780..6800)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6785..7114
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6965..7100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7027..7046
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7162..7209
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7228..7602
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7732..7853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7769..7788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7823..7842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7902..7918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7902..7918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7915..7937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7926..7942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7950..7956,7957..7974,7975..7980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7995..8016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8133..8150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8304..8892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8384..8403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9063..9854,9855..9923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9686..9705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9924..10028)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10103..10122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      721 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      781 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      841 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      901 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      961 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1021 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1081 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1141 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1201 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1261 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1321 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1381 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1441 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1501 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1561 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1621 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1681 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1741 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1801 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1861 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1921 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1981 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2041 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2101 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2161 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2221 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2281 ggcactgcgt gcgccaattc tgcagacaaa tggcagtttc atccacaatt ttaaaagaaa
     2341 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2401 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2461 ggacagcaga gatccagttt ggttaatccg agtgcgtatg agtgcaagtg ggttttagga
     2521 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
     2581 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
     2641 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
     2701 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
     2761 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
     2821 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
     2881 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
     2941 gagcgcagtc gctgcaggga actagtccta ggggtaccgg atcctctaga gtcgactccg
     3001 gagccaccat ggactatggc ggcgctttgt ctgccgtcgg acgcgaactt ttgttcgtta
     3061 ctaatcctgt ggtggtgaac gggtccgtcc tggtccctga ggatcaatgt tactgtgccg
     3121 gatggattga atctcgcggc acgaacggcg ctcagaccgc gtcaaatgtc ctgcagtggc
     3181 ttgcagcagg attcagcatt ttgctgctga tgttctatgc ctaccaaacc tggaaatcta
     3241 catgcggctg ggaggagatc tatgtgtgcg ccattgaaat ggttaaggtg attctcgagt
     3301 tcttttttga gtttaagaat ccctctatgc tctaccttgc cacaggacac cgggtgcagt
     3361 ggctgcgcta tgcagagtgg ctgctcactt gtcctgtcat ccttatccgc ctgagcaacc
     3421 tcaccggcct gagcaacgac tacagcagga gaaccatggg actccttgtc tcagacatcg
     3481 ggactatcgt gtggggggct accagcgcca tggcaaccgg ctatgttaaa gtcatcttct
     3541 tttgtcttgg attgtgctat ggcgcgaaca cattttttca cgccgccaaa gcatatatcg
     3601 agggttatca tactgtgcca aagggtcggt gccgccaggt cgtgaccggc atggcatggc
     3661 tgtttttcgt gagctggggt atgttcccaa ttctcttcat tttggggccc gaaggttttg
     3721 gcgtcctgag cgtctatggc tccaccgtag gtcacacgat tattgatctg atgagtaaaa
     3781 attgttgggg gttgttggga cactacctgc gcgtcctgat ccacgagcac atattgattc
     3841 acggagatat ccgcaaaacc accaaactga acatcggcgg aacggagatc gaggtcgaga
     3901 ctctcgtcga agacgaagcc gaggccggag ccgtgccagc ggccgccacc atggtgagca
     3961 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4021 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4081 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4141 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     4201 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4261 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4321 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4381 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4441 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4501 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     4561 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4621 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gaattctgca
     4681 gtcgacaacc ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc
     4741 tgattatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4801 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4861 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4921 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4981 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5041 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5101 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5161 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5221 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5281 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5341 atcgataccg tcgacctcga cctagaaaaa catggagcaa tcacaagtag caatacagca
     5401 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5461 gtcacacctc gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5521 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     5581 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     5641 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     5701 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     5761 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     5821 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     5881 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5941 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6001 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     6061 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6121 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6181 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6241 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6301 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     6361 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6421 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6481 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6541 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6601 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6661 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6721 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     6781 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6841 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7021 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7081 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7141 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7201 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     7261 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     7321 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     7381 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     7441 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     7501 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     7561 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     7621 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7681 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7861 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7921 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7981 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8041 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8101 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10021 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    10081 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    10141 tgctccctgc ttgtgtgttg gaggtc
//
