LOCUS       sequence-389365-       10557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for the neuronal expression of 
            mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  TITLE     Tools for analysing neuronal cultures in vitro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-hSyn1-mRuby2-T2A-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        117..496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     651..671
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             714..894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    941..1066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1559..1792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1977..2021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2318..2435
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2490..2937
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2973..2982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2979..3689
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             3699..3755
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3756..3761,3762..3779,3780..3782,3783..3815,3816..3818,
                     3819..3842,3843..3869,3870..3926,3927..3932,3933..4202,
                     4203..4220,4221..4655,4656..4661,4662..5105)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKT
                     GHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      3849..3858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4139..4160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4266..4287)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4527..4546)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5130..5718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5183..5203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5601..5612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5721..5737)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5722..5738)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6241..6421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6447..6464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6453..6677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6723..7151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6810..6829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7020..7041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7160..7180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7165..7494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7345..7480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7407..7426
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7542..7589
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7608..7982
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8112..8233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8149..8168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8203..8222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8282..8298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8282..8298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8295..8317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8306..8322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8330..8336,8337..8354,8355..8360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8375..8396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8513..8530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8684..9272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8764..8783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9443..10234,10235..10303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10066..10085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10304..10408)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10483..10502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
       61 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      121 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      541 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      601 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      661 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      901 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      961 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1021 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1081 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1141 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1201 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1261 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1321 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1381 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1441 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1501 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1561 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1621 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1681 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1741 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1801 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1861 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1921 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1981 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2041 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2101 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2161 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2221 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2281 cgccaattct gcagacaaat ggcagtttca tccacaattt taaaagaaaa ggggggattg
     2341 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2401 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2461 atccagtttg gttaatccga gtgcgtatga gtgcaagtgg gttttaggac caggatgagg
     2521 cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc
     2581 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
     2641 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
     2701 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
     2761 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
     2821 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
     2881 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
     2941 aattcaagct gctagctagt accggtccta gggccaccat ggtgtctaag ggcgaagagc
     3001 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat
     3061 tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca
     3121 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt
     3181 atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct
     3241 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg
     3301 tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg
     3361 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata
     3421 cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag
     3481 ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg
     3541 tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg
     3601 aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc
     3661 ttggtggtgg gatggacgag ctgtacaagg gatccggagc cacgaacttc tctctgttaa
     3721 agcaagcagg agacgtggaa gaaaaccccg gtcctggttc tcatcatcat catcatcatg
     3781 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
     3841 aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag
     3901 tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga
     3961 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     4021 tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4081 accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca
     4141 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4201 agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4261 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4321 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4381 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4441 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca
     4501 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4561 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4621 acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc
     4681 agatcgcaga atttaaagag gaattctccc tatttgacaa ggacggggat gggacaataa
     4741 caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc
     4801 tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt
     4861 tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa attagagaag
     4921 cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg
     4981 tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag
     5041 cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga
     5101 agtaagcggc cgcactagag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     5161 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5221 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5281 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5341 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5401 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5461 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5521 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5581 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5641 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5701 tttgggccgc ctccccgcat cgataccgtc gacctcgacc tagaaaaaca tggagcaatc
     5761 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5821 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     5881 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5941 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6001 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     6061 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     6121 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     6181 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     6241 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6301 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6361 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6421 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6481 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6541 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6601 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6661 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6721 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7021 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7081 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7141 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7501 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     7561 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     7621 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     7681 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     7741 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     7801 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     7861 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     7921 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     7981 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8041 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8101 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8221 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8281 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8341 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8401 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10441 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    10501 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtg
//
