LOCUS       sequence-389366-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible CSK-P2A-mCherry expression construct cloned into a 
            lentiviral backbone containing a blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Hamilton MC, Fife JD, Akinci E, Yu T, Khowpinitchai B, Cha M, Barkal
            S, Thi TT, Yeo GHT, Barroso JPR, Francoeur MJ, Velimirovic M, 
            Gifford DK, Lettre G, Yu H, Cassa CA, Sherwood RI
  TITLE     Systematic elucidation of genetic mechanisms underlying cholesterol 
            uptake.
  JOURNAL   bioRxiv. 2023 Jan 10:2023.01.09.500804. doi: 
            10.1101/2023.01.09.500804. Preprint.
  PUBMED    36711952
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jan 10:2023.01.09.500804. doi: 10.1101/2023.01.09.500804. 
            Preprint."
COMMENT     Sequence Label: lentiTet-CSK-P2A-mCherry BlastR
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        357..395
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     392..416
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1802..1858
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1865..2572
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2010..2028)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2278..2297
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2586..3096
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3015..3033
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3106..3501
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3502..3555
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3556..4173,4174..4179,4180..4215,4216..4218,4219..4254,
                     4255..4257,4258..4293,4294..4302)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4342..4362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4760..4771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4976..5209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5281..5402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5318..5337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5372..5391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5442..5577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5577..5648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5649..5717,5718..6509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5867..5886)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6680..7268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7169..7188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7422..7439
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7556..7577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7592..7597,7598..7615,7616..7622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7630..7646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7635..7657
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7654..7670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7654..7670
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7689..7709
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7691..7709
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7737..7963
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7964..8144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8191..8316
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8809..9042
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9227..9271
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
      361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      421 ggagaattgg ctagccacca tgtcagcaat acaggccgcc tggccatccg gtacagaatg
      481 tattgccaag tacaacttcc acggcactgc cgagcaggac ctgcccttct gcaaaggaga
      541 cgtgctcacc attgtggccg tcaccaagga ccccaactgg tacaaagcca aaaacaaggt
      601 gggccgtgag ggcatcatcc cagccaacta cgtccagaag cgggagggcg tgaaggcggg
      661 taccaaactc agcctcatgc cttggttcca cggcaagatc acacgggagc aggctgagcg
      721 gcttctgtac ccgccggaga caggcctgtt cctggtgcgg gagagcacca actaccccgg
      781 agactacacg ctgtgcgtga gctgcgacgg caaggtggag cactaccgca tcatgtacca
      841 tgccagcaag ctcagcatcg acgaggaggt gtactttgag aacctcatgc agctggtgga
      901 gcactacacc tcagacgcag atggactctg tacgcgcctc attaaaccaa aggtcatgga
      961 gggcacagtg gcggcccagg atgagttcta ccgcagcggc tgggccctga acatgaagga
     1021 gctgaagctg ctgcagacca tcgggaaggg ggagttcgga gacgtgatgc tgggcgatta
     1081 ccgagggaac aaagtcgccg tcaagtgcat taagaacgac gccactgccc aggccttcct
     1141 ggctgaagcc tcagtcatga cgcaactgcg gcatagcaac ctggtgcagc tcctgggcgt
     1201 gatcgtggag gagaagggcg ggctctacat cgtcactgag tacatggcca aggggagcct
     1261 tgtggactac ctgcggtcta ggggtcggtc agtgctgggc ggagactgtc tcctcaagtt
     1321 ctcgctagat gtctgcgagg ccatggaata cctggagggc aacaatttcg tgcatcgaga
     1381 cctggctgcc cgcaatgtgc tggtgtctga ggacaacgtg gccaaggtca gcgactttgg
     1441 tctcaccaag gaggcgtcca gcacccagga cacgggcaag ctgccagtca agtggacagc
     1501 ccctgaggcc ctgagagaga agaaattctc cactaagtct gacgtgtgga gtttcggaat
     1561 ccttctctgg gaaatctact cctttgggcg agtgccttat ccaagaattc ccctgaagga
     1621 cgtcgtccct cgggtggaga agggctacaa gatggatgcc cccgacggct gcccgcccgc
     1681 agtctatgaa gtcatgaaga actgctggca cctggacgcc gccatgcggc cctccttcct
     1741 acagctccga gagcagcttg agcacatcaa aacccacgag ctgcacctga ccggtagcgg
     1801 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctac
     1861 gcgtatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     1921 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     1981 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2041 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2101 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2161 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2221 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2281 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2341 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2401 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2461 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2521 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agatcgttta
     2581 attaaggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2641 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2701 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     2761 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     2821 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     2881 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     2941 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     3001 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     3061 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatggc caagcctttg
     3121 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     3181 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     3241 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     3301 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     3361 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     3421 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     3481 tctggttatg tgtgggaggg cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     3541 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     3601 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     3661 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     3721 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     3781 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     3841 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     3901 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     3961 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     4021 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     4081 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     4141 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     4201 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     4261 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     4321 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4381 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4441 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4501 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4561 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4621 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4681 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4741 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4801 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4861 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     4921 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4981 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5221 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     5581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7441 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7561 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7621 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7681 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7741 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7801 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7861 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7921 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8161 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8221 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8281 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8341 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8401 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8461 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8521 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8581 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8641 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8701 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8761 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8821 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8881 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8941 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9001 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9061 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9121 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9181 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9241 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9301 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9361 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9421 ccacctccca accccgaggg gac
//
