LOCUS       sequence-389384-       11876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing
            cDNA for a codon-optimized puromycin resistance gene, followed by an
            FMDV 2A domain and then EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11876)
  TITLE     Transposon vectors for proximity biotin labeling with split TurboID
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHR_SFFV*/Puro2a-PAG1-GFP
FEATURES             Location/Qualifiers
     source          1..11876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1057..1122
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1252..1272
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1697..1831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1734..1753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1788..1807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1999..2632
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2679..2804
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3301..3534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3719..3763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4048..4165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4318..4725
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      4802..4811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5516..5581
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(6905..6907,6908..7621)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6947..6968)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7208..7227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7555..7576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7736..8324
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7789..7809)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8207..8218)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8327..8343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8328..8344)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8434..8667
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8692..8710)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8693..8710)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9000..9019)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9233..9252)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9352..9374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9412..9430)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9498..9602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9603..9671,9672..10463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9821..9840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10634..11222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11123..11142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11376..11393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11463..11483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11468..11799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11648..11785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
       61 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
      121 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
      181 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
      241 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
      301 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
      361 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
      421 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
      481 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
      541 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
      601 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
      661 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
      721 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
      781 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
      841 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
      901 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
      961 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     1021 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     1081 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     1141 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     1201 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     1261 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     1321 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     1381 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     1441 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     1501 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     1561 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     1621 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1681 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1801 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     1861 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     1921 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     1981 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     2041 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     2101 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     2161 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     2221 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     2281 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     2341 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     2401 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     2461 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2521 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2581 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2641 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2701 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2761 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2821 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2881 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2941 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     3001 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     3061 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     3121 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     3181 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     3241 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     3301 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     3361 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     3421 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     3481 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     3541 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     3601 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     3661 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     3721 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     3781 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     3841 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     3901 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     3961 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     4021 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4081 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4141 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     4201 gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat tttatttagt
     4261 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     4321 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     4381 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     4441 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     4501 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     4561 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     4621 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     4681 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga tctggagctc
     4741 tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc aggcctctga
     4801 ggccaccatg gcgacggagt ataagccgac tgttaggttg gccacgcgcg acgacgttcc
     4861 gagagcagtg cggactttgg cggcagcctt cgcagactat ccggctacac gccacaccgt
     4921 cgacccggat cgccatattg agcgagttac ggaacttcag gaactttttc ttactagagt
     4981 gggcctggac attggtaaag tctgggtagc tgatgacggt gccgcagtgg ccgtctggac
     5041 cactcccgaa agcgttgaag ccggagccgt ctttgctgaa atcggcagcc gcatggcgga
     5101 gctttccggt tccagactcg ccgcgcaaca acaaatggaa ggtctcctgg ctccccatag
     5161 accaaaggaa ccagcgtggt tccttgcgac tgtcggagtc tctcccgacc accaaggcaa
     5221 ggggctgggg tccgcagtag tccttccagg cgtagaagct gcggaacgag caggagtccc
     5281 tgcgttcctg gagacatcag cccctcgcaa tcttccgttt tatgagaggt tgggattcac
     5341 ggtgacagcc gatgtagaag taccagaagg accgcgaacc tggtgcatga ccaggaagcc
     5401 gggtgccgtg accgagcttc tgtaccgaat gaagcgggcg gagacatact gcccaaggcc
     5461 acttctggct attcatccga ccgaagcccg ccacaaacaa aagatcgtag ctcccgttaa
     5521 acagacgctg aattttgacc ttctgaaact ggctggtgac gtggaatcca accctggacc
     5581 cggtcctgcg ggaagcctgc tgggcagcgg acagatgcag atcaccctgt ggggaagtct
     5641 ggctgctgtc gccattttct tcgtcatcac cttcctcatc ttcctgtgct ctagttgtga
     5701 cagggaaaag aagccgcgac agcatagtgg ggaccatgag aacctgatga acgtgccttc
     5761 agacaaggag atgttcagcc gttcagttac tagcctggca acagatgctc ctgccagcag
     5821 tgagcagaat ggggcactca ccaatgggga cattctttca gaggacagta ctctgacctg
     5881 catgcagcat tacgaggaag tccagacatc ggcctcggat ctgctggatt cccaggacag
     5941 cacagggaaa ccaaaatgtc atcagagtcg ggagctgccc agaatccctc ccgagagcgc
     6001 agtggatacc atgctcacgg cgagaagtgt ggacggggac caggggctgg ggatggaagg
     6061 gccctatgaa gtgctcaagg acagctcctc ccaagaaaac atggtggagg actgcttgta
     6121 tgaaactgtg aaagagatca aggaggtggc tgcagctgca cacctggaga aaggccacag
     6181 tggcaaggca aaatctactt ctgcctcgaa agagctccca gggccccaga ctgaaggcaa
     6241 agctgagttt gctgaatatg cctcggtgga cagaaacaaa aaatgtcgtc aaagtgttaa
     6301 tgtagagagt atccttggaa attcatgtga tccagaagag gaggccccac cacctgtccc
     6361 tgttaagctt ctggacgaga atgaaaacct tcaggagaag gaagggggag aggcggaaga
     6421 gagtgccaca gacacgacca gtgaaactaa caagagattt agctcattgt catacaagtc
     6481 tcgggaagaa gaccccactc tcacagaaga agagatctca gctatgtact catcagtaaa
     6541 taaacctgga cagttagtga ataaatcggg gcagtcgctt acagttccgg agtccaccta
     6601 cacctccatt caaggggacc cacagaggtc accctcctcc tgtaatgatc tctatgctac
     6661 tgttaaagac ttcgaaaaaa ctccaaacag cacacttcca ccagcaggga ggcccagcga
     6721 ggagccagag cctgattatg aagcgataca gactctcaac agagaggaag aaaaggccac
     6781 cctggggacc aatggccacc acggtctcgt cccaaaggag aacgactacg agagcataag
     6841 tgacttgcag caaggcagag atattaccag gctcggagga ggcagcggtg gtggaagtgg
     6901 acccgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     6961 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7021 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     7081 cctcgtgacc accttcacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     7141 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     7201 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     7261 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     7321 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     7381 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     7441 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     7501 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     7561 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     7621 aggcctgtca ggccaagcgc ggccgcaact cccacctgca acatgcgtga ctgactgagg
     7681 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     7741 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7801 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7861 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7921 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7981 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8041 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8101 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8161 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8221 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8281 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     8341 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     8401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8461 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8641 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
     8701 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     8761 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     8821 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     8881 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     8941 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     9001 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     9061 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     9121 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     9181 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     9241 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     9301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     9421 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11401 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    11521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    11581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    11641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    11701 aatttttttt atttatgcag aggccgaggt accgcctcgg cctctgagct attccagaag
    11761 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    11821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgt
//
