LOCUS       sequence-389398-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCI-Puro_hCOL4A4-Myc-His
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6228..6248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             6273..6302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6312..6341
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6351..6380
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6390..6419
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6429..6458
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6486..6503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6540..7128
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6593..6613)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7011..7022)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7216..7234)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7271..7392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7282..7301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7336..7355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7637..7994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7637..7828
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7660..7680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7845..7980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8025..8624
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8025..8044)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8521..8541
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8649..8697
                     /note="synthetic polyadenylation signal"
     promoter        8853..8957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8958..9026,9027..9818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9176..9195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9976..10564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10056..10075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10680..10963
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgccg aactcaccta
     1081 tctccccaac acctaataac attcaatcac tctttccact aaccacctat ctacatcacc
     1141 acccaccagc cgccaccatg tggtctctgc acatagtact aatgaggtgc tccttcagat
     1201 tgaccaagtc cttggccaca ggtccctggt cacttatact cattctcttt tctgtacaat
     1261 atgtatatgg gagtggaaag aaatacattg gtccttgtgg aggaagagat tgctctgttt
     1321 gccactgtgt tcctgaaaag gggtctcggg gtccaccagg accaccaggg ccacagggtc
     1381 caattggacc cctgggagcc ccaggaccca ttgggctttc aggagagaaa ggaatgagag
     1441 gggaccgcgg ccctcctgga gcagcagggg acaaaggaga taagggtcca actggtgttc
     1501 ctggatttcc aggtttagat ggcatacctg ggcacccagg gcctcctgga cccagaggca
     1561 aacctggtat gagtggccac aatggctcaa gaggtgaccc agggtttcca ggaggaagag
     1621 gagctcttgg cccaggaggc cccctaggcc atcctgggga aaagggagaa aaaggaaatt
     1681 cagtgttcat tttaggtgcc gttaaaggta ttcagggaga cagaggggac ccaggactgc
     1741 ctggcttacc aggatcttgg ggtgcaggag gaccggcagg tcccacagga tatcctggag
     1801 agccagggtt agtgggacct ccgggccaac cagggcgtcc aggtttgaag ggaaatcccg
     1861 gtgtgggagt aaaggggcaa atgggagacc cgggtgaggt tggtcagcaa ggttctcctg
     1921 gacccaccct gttggtagag ccacctgact tttgtctcta taaaggagaa aagggtataa
     1981 aaggaattcc tggaatggtt ggactgccag gaccaccagg acgcaaggga gaatctggta
     2041 ttggggcaaa aggagaaaaa ggtattcctg gatttccagg gcctcggggg gatcctggtt
     2101 cctatggatc tccaggtttt ccaggattaa agggagaact aggactggtt ggagatcctg
     2161 ggctatttgg attaattggc ccaaaggggg atcctggaaa tcgagggcac ccaggaccac
     2221 caggtgtttt ggtgactcca cctcttccac tcaaaggccc accaggggac ccagggttcc
     2281 ctggccgcta tggagaaaca ggggatgttg gaccacctgg tcccccaggt ctcttgggca
     2341 gaccagggga agcctgtgca ggcatgatag gaccccctgg gccacaagga tttcctggtc
     2401 ttcctgggct tccaggagaa gctggtattc ctgggagacc tgattctgct ccaggaaaac
     2461 cagggaagcc aggatcacct ggcttgcctg gagcaccagg cctgcagggc ctcccaggat
     2521 caagtgtgat atactgtagt gttgggaacc ccggaccaca aggaataaaa ggcaaagttg
     2581 gtcccccagg aggaagaggc ccaaaaggag aaaaaggaaa tgaaggactc tgtgcctgtg
     2641 agcctggacc catgggcccc cctggccctc caggacttcc tgggaggcag gggagtaagg
     2701 gagacttggg gctccctggc tggcttggaa caaaaggtga cccaggacct cctggtgctg
     2761 aaggacctcc agggctacca ggaaagcatg gtgcctctgg accacctggc aacaaagggg
     2821 cgaagggtga catggttgta tcaagagtta aagggcacaa aggagaaaga ggtcctgatg
     2881 ggcccccagg atttccaggg cagccaggat cacatggtcg ggatggacat gctggagaaa
     2941 aaggggatcc aggacctcca ggggatcatg aagatgcgac cccaggtggt aaaggatttc
     3001 ctggacctct gggcccccca ggcaaagcag gacctgtggg gcccccagga ctgggatttc
     3061 ctggtccacc aggagagcga ggccacccag gagttccagg ccacccaggt gtgaggggcc
     3121 ctgatggctt gaagggtcag aaaggtgaca caatttcttg caacgtaacc taccctggga
     3181 ggcatggccc tccaggtttt gatggacctc caggtccgaa gggatttcca ggtccccaag
     3241 gtgcccctgg gctgagtggt tcagatgggc ataaaggcag acctggcaca ccaggaacag
     3301 cggaaatacc aggtccacct ggttttcgtg gtgacatggg agatccgggt tttggaggtg
     3361 aaaaggggtc ctcccctgtt gggcccccag gccctcccgg ctcaccagga gtgaatggtc
     3421 agaaaggaat cccgggagac cctgcatttg gtcacctggg acccccggga aagaggggtc
     3481 tttcaggagt gccagggata aaaggaccca ggggtgatcc gggatgtcca ggggctgaag
     3541 ggccagctgg cattcctgga ttcctaggtc tcaaaggtcc caaaggcaga gagggacatg
     3601 ctgggtttcc aggtgtccca ggtccacctg gccattcctg tgaaagaggt gctccaggga
     3661 taccagggca accgggactc cctgggtatc caggtagccc aggtgctcca ggtgggaaag
     3721 gacagccggg agatgtgggg cctcccgggc cagctggaat gaaaggcctc cccggactcc
     3781 caggacggcc tggggcacat ggtcccccag gcctcccagg aatcccaggt ccctttggag
     3841 atgatgggct acctggtcct ccaggtccaa agggaccccg ggggctgcct ggtttcccag
     3901 gttttcccgg agaaagagga aagcctggtg cagagggatg tcctggcgca aagggagaac
     3961 ctggagagaa gggcatgtct ggccttcctg gagaccgggg actgagaggg gccaaaggag
     4021 ccataggacc tcccggagat gaaggagaaa tggctatcat ttcacaaaag ggaacacctg
     4081 gggaacctgg acctcctgga gatgatggat tcccaggaga aagaggtgat aaaggaactc
     4141 ccgggatgca agggagaaga ggagagccgg gaagatacgg accacctgga tttcacagag
     4201 gggaacctgg tgagaaaggt cagccagggc ctcctggacc cccaggccct ccaggctcaa
     4261 ctggtctaag agggttcatt ggttttccag gacttccagg tgaccagggt gagccaggtt
     4321 ctccaggtcc ccctggattt tcaggaattg atggagcaag aggacctaaa ggaaacaaag
     4381 gtgaccctgc cagtcacttt ggtccacctg gtccaaaggg tgagccaggt agccctggat
     4441 gtccagggca ttttggagca tccggagagc agggcttgcc tggtattcaa gggcccagag
     4501 gatcacctgg aaggccaggg ccacctggct cctctggacc accagggtgc ccaggtgatc
     4561 acgggatgcc tgggctgagg ggacagccag gagaaatggg agaccctggg ccaagaggcc
     4621 tccaggggga tccagggata ccaggtcctc cgggaataaa aggtccctcc ggatcacctg
     4681 gcctgaacgg cttgcatgga ttgaaaggtc agaaaggaac taaaggtgct tcaggtttgc
     4741 atgatgtggg gccacctggt ccagtgggaa tacctgggct aaaaggggag agaggagacc
     4801 ctgggagccc aggaatctct cctccaggtc ctcgtggaaa gaaaggtccc ccaggacccc
     4861 cagggagttc aggaccacct ggtcctgcag gtgccacagg aagagctcct aaggacattc
     4921 ctgacccggg tccacctgga gatcagggac ctcctggtcc tgatggccca agaggagcac
     4981 ctgggcctcc aggcctccct gggagtgttg accttctgag aggggagcca ggtgactgtg
     5041 gtctaccagg gccaccaggt ccccctggcc caccaggccc tccaggatac aaaggctttc
     5101 caggatgtga tggaaaagat ggccagaaag gaccagtggg attcccggga ccgcagggac
     5161 cacatggatt tcctgggcca cctggagaga agggtttacc tggacctcca gggagaaaag
     5221 ggcccactgg tcttccgggt cccagaggtg aaccggggcc acctgcagat gtggatgact
     5281 gtccccgaat cccaggcctt cctggggcgc caggcatgag aggaccagaa ggagccatgg
     5341 ggctccctgg aatgagaggc ccctcaggac cagggtgtaa aggagagcct gggctggatg
     5401 gcaggagggg tgtggatggc gtccctgggt ctcctgggcc tcccggacgt aaaggtgaca
     5461 caggagaaga cggctaccct ggaggaccag ggcctcctgg tcccattggg gatcctgggc
     5521 ccaaagggtt tggccctgga tacctcggtg gcttcctcct ggttctccac agtcagacgg
     5581 accaggagcc cacctgcccc ctgggcatgc ccaggctctg gactgggtat agtctgttat
     5641 acctggaagg gcaagagaaa gctcacaatc aagaccttgg tctggcaggg tcttgccttc
     5701 ccgtatttag cacgctgccc tttgcctact gcaacatcca ccaggtgtgc cactatgccc
     5761 agagaaacga cagatcctac tggctggcca gcgctgcgcc cctccccatg atgccactct
     5821 ctgaagaggc gatccgcccc tatgtcagcc gctgtgcggt atgcgaggcc ccggcccagg
     5881 cggtggcggt gcacagccag gaccagtcca tccccccatg tccgcagacc tggaggagcc
     5941 tctggatcgg gtattcattc ctgatgcaca caggagctgg ggaccaagga ggagggcagg
     6001 cccttatgtc acctggcagc tgcctggaag atttcagagc agcaccattc cttgaatgcc
     6061 agggccggca gggaacttgc cactttttcg caaataagta tagcttctgg ctcacaacgg
     6121 tgaaagcaga cttgcagttt tcctctgctc cagcaccaga caccttaaaa gaaagccagg
     6181 cccaacgcca gaaaatcagc cggtgccagg tctgcgtgaa gtatagcgag gatctgtact
     6241 ttcagagcat cgatggaagt ggtaaagcta tggagcaaaa gctcatttct gaagaggact
     6301 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
     6361 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga
     6421 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag agcttgggcg
     6481 acctccatca tcaccatcac cactagggga tatcagctga attctagaaa ttcatcgaca
     6541 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6601 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6661 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6721 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6781 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6841 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6901 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6961 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7021 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7081 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgccc aggttccttg
     7141 ggccgcgact ctagggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg
     7201 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga
     7261 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7321 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7381 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     7441 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa
     7501 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7561 tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa
     7621 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     7681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7921 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7981 taggcttttg caaaaagctc gattcttctg acactagcgc caccatgacc gagtacaagc
     8041 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     8101 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     8161 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8221 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8281 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8341 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8401 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8461 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8521 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8581 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctagaactct agccatcacg
     8641 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     8701 cgatagcgat aaggatcctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     8761 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta atggtttctt
     8821 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8881 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8941 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9001 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9061 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9121 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa gttctgctat
     9181 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9241 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9301 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9361 tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9421 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9481 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9541 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9601 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9661 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9721 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9781 tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc gaccaagcga
     9841 cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10381 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt
    10561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttatagtc
    10681 cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac catgaaaaat
    10741 ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt gcataggtat
    10801 tcatacggtt aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat
    10861 ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg
    10921 gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac cgcagtactc
    10981 cggccacgat gcgtccggcg tagaggatcg agatct
//
