LOCUS       sequence-389408-       10553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing
            cDNA for a codon-optimized puromycin resistance gene, followed by an
            FMDV 2A domain and then EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10553)
  TITLE     Transposon vectors for proximity biotin labeling with split TurboID
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHR_SFFV*/Puro2a-GFP
FEATURES             Location/Qualifiers
     source          1..10553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(907..926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1026..1048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1086..1104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1277..1345,1346..2137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1495..1514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2308..2896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2797..2816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3050..3067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3137..3157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3142..3473
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3322..3459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4607..4672
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4802..4822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5247..5381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5284..5303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5338..5357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5549..6182
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6229..6354
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6851..7084
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7269..7313
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7598..7715
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7868..8275
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8352..8361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9066..9131
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(9132..9134,9135..9848)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9174..9195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9435..9454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9782..9803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9963..10551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10016..10036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10434..10445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggtaccgcc tcggcctctg
     3421 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     3481 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     3541 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     3601 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     3661 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     3721 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     3781 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     3841 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     3901 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     3961 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     4021 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     4081 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     4141 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     4201 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     4261 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     4321 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     4381 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     4441 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     4501 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     4561 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     4621 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     4681 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     4741 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     4801 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     4861 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     4921 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     4981 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     5041 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     5101 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     5161 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     5221 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5281 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5341 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     5401 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     6721 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6781 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6841 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6901 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6961 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7021 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7081 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7141 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7201 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7261 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7321 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7381 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7441 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     7501 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     7561 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     7621 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     7681 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7741 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
     7801 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     7861 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
     7921 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
     7981 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     8041 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     8101 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     8161 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     8221 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     8281 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     8341 aggcctctga ggccaccatg gcgacggagt ataagccgac tgttaggttg gccacgcgcg
     8401 acgacgttcc gagagcagtg cggactttgg cggcagcctt cgcagactat ccggctacac
     8461 gccacaccgt cgacccggat cgccatattg agcgagttac ggaacttcag gaactttttc
     8521 ttactagagt gggcctggac attggtaaag tctgggtagc tgatgacggt gccgcagtgg
     8581 ccgtctggac cactcccgaa agcgttgaag ccggagccgt ctttgctgaa atcggcagcc
     8641 gcatggcgga gctttccggt tccagactcg ccgcgcaaca acaaatggaa ggtctcctgg
     8701 ctccccatag accaaaggaa ccagcgtggt tccttgcgac tgtcggagtc tctcccgacc
     8761 accaaggcaa ggggctgggg tccgcagtag tccttccagg cgtagaagct gcggaacgag
     8821 caggagtccc tgcgttcctg gagacatcag cccctcgcaa tcttccgttt tatgagaggt
     8881 tgggattcac ggtgacagcc gatgtagaag taccagaagg accgcgaacc tggtgcatga
     8941 ccaggaagcc gggtgccgtg accgagcttc tgtaccgaat gaagcgggcg gagacatact
     9001 gcccaaggcc acttctggct attcatccga ccgaagcccg ccacaaacaa aagatcgtag
     9061 ctcccgttaa acagacgctg aattttgacc ttctgaaact ggctggtgac gtggaatcca
     9121 accctggacc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     9181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     9241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     9301 ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc
     9361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     9421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9541 tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc
     9601 agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc
     9661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     9721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     9781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9841 acaagtaagg cctgtcaggc caagcgcggc cgcaactccc acctgcaaca tgcgtgactg
     9901 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     9961 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10021 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10081 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10141 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10201 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10261 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10321 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    10381 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10441 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10501 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cat
//
