LOCUS       sequence-389420-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA
            for the encoding of non-canonical amino acids at TAG codons in 
            HEK293 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Avila-Crump S, Hemshorn ML, Jones CM, Mbengi L, Meyer K, Griffis JA,
            Jana S, Petrina GE, Pagar VV, Karplus PA, Petersson EJ, Perona JJ, 
            Mehl RA, Cooley RB
  TITLE     Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA 
            Synthetases for Structurally Diverse Non-Canonical Amino Acids.
  JOURNAL   ACS Chem Biol. 2022 Dec 16;17(12):3458-3469. doi: 
            10.1021/acschembio.2c00639. Epub 2022 Nov 16.
  PUBMED    36383641
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.2c00639"; journalName: "ACS Chem Biol"; date: 
            "2022-12-16- 16"; volume: "17"; issue: "12"; pages: "3458-3469"
COMMENT     Sequence Label: pAcBac1-Ma-sfGFP WT
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..265
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        342..436
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    418..436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(926..931,932..948,949..954)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1143..1676
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  1743..1967
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2101..2118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2272..2860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2352..2371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3031..3822,3823..3891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3654..3673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3892..3996)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4022..4476)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4159..4180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4370..4389
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  4660..4825
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(4918..5012)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    4918..4936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4946..4964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(5089..5337)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    5370..5504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5394..5413)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5448..5467
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(5650..7137,7138..7185))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     promoter        complement(7244..7335)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(7302..7319)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     enhancer        7430..7809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7810..8013
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7964..7984
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8058..8077
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8058..8076
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(8099..8101,8102..8104,8105..8815)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             8822..8863
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8864..8881
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    8907..9495
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8960..8980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9378..9389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9544..9561)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9550..9774
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agatctcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccggggg acggtccggc gaccagcggg tctctaaaac
      301 ctagcatagc ggggttcgac accccggtct ctcgtttttt aatatttgca tgtcgctatg
      361 tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc
      421 ctatcagtga tagagacacc gggggacggt ccggcgacca gcgggtctct aaaacctagc
      481 atagcggggt tcgacacccc ggtctctcgt tttttcagct ggggctctag ggggtatccc
      541 cacgcgccct gtagcggcgc attaagcgca agccctgcca tagccactac gggtacgtag
      601 ccaaccacta gaactatagc tagagtcctg ggcgaacaaa cgatgctcgc cttccagaaa
      661 accgaggatg cgaaccactt catccggggt cagcaccacc ggcaagcgcc gcgacggccg
      721 aggtctaccg atctcctgaa gcaagggcag atccgtgcac agcaccttgc cgtagaagaa
      781 cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag
      841 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
      901 gaaacggatg aaggcacgaa cccagttgac ataagcctgt tcggttcgta aactgtaatg
      961 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1021 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg
     1081 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1141 cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
     1201 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca
     1261 tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac
     1321 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
     1381 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
     1441 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc
     1501 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
     1561 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
     1621 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat
     1681 tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacgcc
     1741 gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg
     1801 cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca
     1861 gcataactgg actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat
     1921 agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag
     1981 ggcatccatt tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtctatgcg
     2041 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     2101 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2161 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2221 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2701 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2821 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2881 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2941 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3001 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3061 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3121 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3181 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3241 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3301 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3361 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3421 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3481 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3541 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3601 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3661 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3721 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3781 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3841 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3901 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3961 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4021 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4081 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4141 atagggttga gtgttgttcc agtttggaac aagagtccac tattaagaac gtggactcca
     4201 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4261 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4321 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4381 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4441 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcaaa
     4501 taagcgttga tattcagtca attacaaaca ttaataacga agagatgaca gaaaaatttt
     4561 cattctgtga cagagaaaaa gtagccgaag atgacggttt gtcacatgga gttggcagga
     4621 tgtttgatta aaaacataac aggaagaaaa atgccccgct gtgggcggac aaaatagttg
     4681 ggaactggga ggggtggaaa tggagttttt aaggattatt tagggaagag tgacaaaata
     4741 gatgggaact gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt
     4801 ggaactagat ttcacttatc tggttcggat ctcctagcaa aaaacgagag accggggtgt
     4861 cgaaccccgc tatgctaggt tttagagacc cgctggtcgc cggaccgtcc cccggtgtct
     4921 ctatcactga tagggaactt ataagtctct atcactgata gggatttcac gtttatggtg
     4981 atttcccaga acacatagcg acatgcaaat attaaaaaac gagagaccgg ggtgtcgaac
     5041 cccgctatgc taggttttag agacccgctg gtcgccggac cgtcccccgg tgtttcgtcc
     5101 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
     5161 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
     5221 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
     5281 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcttc
     5341 ctgcccgacc taggctcaag cagtgatcag atccagacat gataagatac attgatgagt
     5401 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     5461 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     5521 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     5581 tctacaaatg tggtatggct gattatgatc ctctagtact tctcgacaag cttggccggc
     5641 ctggccttat cactttccaa gtcggttcat ctctatgtct gtataaatct gtcttttctt
     5701 ggtgtgcttt aatttaatgc aaagatggat accaactcgg agaaccaaga atagtccaat
     5761 gattaaccct atgataaaga aaaaagaggc aatagagctt ttccaactac tgaaccaacc
     5821 ttctacaagc tcgattggat ttttggatag cccagtatca ccaaaaaata aactctcatc
     5881 atcaggaagt tgcgaagcag cgtcttgaat gtgaggatgt tcgaacacct gagcctttga
     5941 gctaagatga agatcggagt ccaacatacc atgtccaatc atgtataaag gaaacttata
     6001 tcctgaactg gtcctcagaa ctccattggg tccaatttcc acgtcttcat atggtgccca
     6061 gtcatcccac agttcccttt ctgtggtagt tccactgatc attccgacca ttcttgagag
     6121 gattggagca gcaatatcga ctctgatgta tctggtctca aagtatttta gggtaccatt
     6181 gattatggtg aaagcaggac cggttcctgg gtttttagga gcaagatagc tgagatccac
     6241 tggagagatt ggaagacccg ctctgatttt gctccaggtt tcttggcaga gggaataatc
     6301 caagatcctc tcaacgtcct gaattagact tacatccact gaggtctgag atggagcaga
     6361 gatacttgac ccttctgggc attcagggaa tctggctgca gcaaagagat ccttatcagc
     6421 catctcgaac cagacacctg atgggagtct gactccccaa tgcttgcagt attgcatttt
     6481 gcaggccttg cctccagttt cataagcaaa gtagttactt ctgaaccctg tgccctcctt
     6541 tcccagggat gatagctctc cgtcctctga gaagaaggtg atgtccatgg aaatgaggtt
     6601 agaatcacat agccctttga ccttatagtc agaatgccag gttgtagagt tatggacagt
     6661 ggggcatatg taattgctgc attttccgtt gatgaactgt gaatcaaccc attctcctgt
     6721 gtattcatca accagcacat ggtgaggagt cacctggaca atcactgctt cggcatccgt
     6781 cacagttgca tatccacaac tttgaggagg gaagcctgga ttcagccaag ttccttgttt
     6841 cgtttgttca atgctttcct tgcattgttc tacagatgga gtgaaggatc ggatggaatg
     6901 tgttatatac ttcggtccat accagcggaa atcacaagta gtgacccatt tggaagcatg
     6961 acacatccaa ccgtctgctt gaatagcctt gtgactcttg ggcattttga cttgtaaggc
     7021 tgtgcctatt aagtcattat gccaatttaa atctgagctt gacgggcaat aatggtaatt
     7081 agaaggaaca tttttccagt ttcctttttg gttgtgtgga aaaactatgg tgaacttgca
     7141 attcacccca atgaataaaa aggctaagta caaaaggcac ttcatggtgg cggtttttta
     7201 ggagttcgcg cccgatggtg ggacggtatg aataatccgg aatatttata ggttttttta
     7261 ttacaaaact gttacgaaaa cagtaaaata cttatttatt tgcgagatgg ttatcatttt
     7321 aattatctcc atgatctatt aatattccgg agtatacgga ccgcggccgc aaatacctgc
     7381 aggatccgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     7441 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7501 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7561 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7621 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7681 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7741 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7801 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7861 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7921 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7981 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     8041 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcaccat
     8101 ggtttctaaa ggtgaagaac tttttactgg cgttgtgccg attctggtgg aactggatgg
     8161 tgatgtgaat ggccataaat ttagcgttcg tggcgaaggc gaaggtgatg cgaccaacgg
     8221 taaactgacc ctgaaattta tttgcaccac cggtaaactg ccggttccgt ggccgaccct
     8281 ggtgaccacc ctgacctatg gcgttcagtg ctttagccgc tatccggatc atatgaaacg
     8341 ccatgatttc tttaaaagcg cgatgccgga aggctatgtg caggaacgta ccattagctt
     8401 caaagatgat ggcacctata aaacccgtgc ggaagttaaa tttgaaggcg ataccctggt
     8461 gaaccgcatt gaactgaaag gtattgattt taaagaagat ggcaacattc tgggtcataa
     8521 actggaatat aatttcaaca gccataatgt gtatattacc gccgataaac agaaaaatgg
     8581 catcaaagcg aactttaaaa tccgtcacaa cgtggaagat ggtagcgtgc agctggcgga
     8641 tcattatcag cagaataccc cgattggtga tggcccggtg ctgctgccgg ataatcatta
     8701 tctgagcacc cagagcgttc tgagcaaaga tccgaatgaa aaacgtgatc atatggtgct
     8761 gctggaattt gttaccgccg cgggcattac ccacggtatg gatgaacttt ataaaggttc
     8821 tggaaagccg attccaaatc ccctgttggg actggattct actcatcacc accatcacca
     8881 ctaagaattc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     8941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     9001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9301 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9481 gggccgcctc cccgcgtcga ctttaactcg agtctagagg gcccgtttaa acccgctgat
     9541 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9601 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9661 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9721 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     9781 aggcggaaag aacc
//
