LOCUS       sequence-389424-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive selection plasmid for selecting ncAA-encoding 
            Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon 
            interrupted chloramphenicol resistance cassette and M. alvus 
            Pyl-tRNA(6). p15a ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Avila-Crump S, Hemshorn ML, Jones CM, Mbengi L, Meyer K, Griffis JA,
            Jana S, Petrina GE, Pagar VV, Karplus PA, Petersson EJ, Perona JJ, 
            Mehl RA, Cooley RB
  TITLE     Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA 
            Synthetases for Structurally Diverse Non-Canonical Amino Acids.
  JOURNAL   ACS Chem Biol. 2022 Dec 16;17(12):3458-3469. doi: 
            10.1021/acschembio.2c00639. Epub 2022 Nov 16.
  PUBMED    36383641
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.2c00639"; journalName: "ACS Chem Biol"; date: 
            "2022-12-16- 16"; volume: "17"; issue: "12"; pages: "3458-3469"
COMMENT     Sequence Label: pPos-Ma-CmR-TAG111
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      363..908
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     809..828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1025..1042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1434..1536
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1537..2196
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYH*DFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1603..1622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3042..3058)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3051..3073)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(3380..4570)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     4199..4218
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(4618..4623,4624..4640,4641..4646))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(5042..5128)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5281..5298
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(8031..8315)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(8089..8108)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             8342..9220
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(9376..9398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     terminator      complement(9418..9465)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     9461..9479
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(9515..10231)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(9558..9578)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     10173..10201
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             10239..10261
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(10292..10311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10293..10311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
       61 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
      361 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
      421 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
      481 ggaggagcga agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
      541 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
      601 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
      661 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
      721 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      781 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
      841 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
      901 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
      961 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     1021 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     1081 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     1141 ccaacatagt aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt
     1201 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
     1261 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca
     1321 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt
     1381 gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg
     1441 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
     1501 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1561 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1621 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1681 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1741 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1801 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1861 taccactagg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1921 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1981 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2041 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2101 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2161 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     2221 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     2281 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
     2341 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
     2401 atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca
     2461 tttattctgc ctcccagagc atgataaaaa cggttagcgc ttcgttaata cagatgtagg
     2521 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2581 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgctgc
     2641 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     2701 catgccccgc gcccaccgga aggagctacc ggcagcggtg cggactgttg taactcagaa
     2761 taagaaatga ggccgctcat ggcgttctgt tgcccgtctc actggtgaaa agaaaaacaa
     2821 ccctggcgcc gcttctttga gcgaacgatc aaaaataagt ggcgccccat caaaaaaata
     2881 ttctcaacat aaaaaacttt gtgtaatact tgtaacgcta gatctggggg acggtccggc
     2941 gaccagcggg tctctaaaac ctagcatagc ggggttcgac accccggtct ctcgccaact
     3001 agtatcctta gcgaaagcta aggatttttt ttaagcttgg cactggccgt cgttttacaa
     3061 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3121 ttcgccagac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     3181 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagcccga gatgcgccgc
     3241 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     3301 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
     3361 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg
     3421 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc
     3481 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg
     3541 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
     3601 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
     3661 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
     3721 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     3781 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     3841 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     3901 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     3961 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg
     4021 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     4081 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     4141 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     4201 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     4261 tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     4321 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     4381 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     4441 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     4501 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     4561 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     4621 aaagcttatc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     4681 acttcaggtg ctacattgct caaagatgca ggggtaaaag ctaaccgcat ctttaccgac
     4741 aaggcatccg gcagttcaac agatcgggaa gggctggatt tgctgaggat gaaggtggag
     4801 gaaggtgatg tcattctggt gaagaagctc gaccgtcttg gccgcgacac cgccgacatg
     4861 atccaactga taaaagagtt tgatgctcag ggtgtagcgg ttcggtttat tgacgacggg
     4921 atcagtaccg acggtgatat ggggcaaatg gtggtcacca tcctgtcggc tgtggcacag
     4981 gctgaacgcc ggaggatcct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
     5041 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     5101 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
     5161 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
     5221 gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
     5281 gatttaatct gtatcaggct gaaaatcttc tctcatccga cgtcttaggc gaaggcgaag
     5341 tccgactcta agatgtcacg gaggttcaag ttacctttag ccggaagtgc tggcattttg
     5401 tccaattgag actcgtgcaa ctggtcagcg aactggtcgt agaaatcagc cagtacatca
     5461 caagactcat atgtgtcaac catagtttcg cgcactgctt tgaacaggtt cgcagcgtca
     5521 gccggaatgg taccgaagga gtcgtgaatc agtgcaaaag attcgattcc gtacttctcg
     5581 tgtgcccaca ctacagtctt acgaaggtgg ctaccgtctt ggctgtgtac aaagttagga
     5641 gcgataccag actcctgttt gtgtgcatca atctcgctat ctttgttggt gttaatggta
     5701 ggctgtaagc ggaactgacc gaggaacatc aggttcaagc gcgtctgaat aggcttcttg
     5761 tattcctgcc acacagggaa accatcagga gttacccaat gcacagcgca acgcttgcga
     5821 agaatctctc cagtcttctt atctttgacc tcagcagcca gcagcttagc agcagactta
     5881 agccagttca ttgcttcaac cgcagctacc accgtcacgc tcacagattc ccaaatcagc
     5941 ttagccatgt atccagcagc ctgattcggc tgagtgaaca tcagaccctt gccggaatca
     6001 atagctggct gaatggtatc ttccagcact tgttgacgga agccgaactc tttggacccg
     6061 taagccagcg tcatgactga acgcttagtc acactgcgag taacaccgta agccagccat
     6121 tgaccagcca gtgccttagt gcccagcttg actttctcag agatttcacc agtgttctca
     6181 tcggtcacgg taactacttc gttatcggtc ccattgattg cgtctgcttg tagaatctcg
     6241 ttgactttct tagcaacaat cccgtagatg tcctgaacgg tttcactagg aagcaagtta
     6301 accgcgcgac cacctacctc atctcggagc atcgcggaga agtgctggat gccagagcaa
     6361 gacccgtcaa acgccagcgg aagggagcag ttatagctca ggccgtggtg ctgtacccca
     6421 gcgtactcaa agcagaacgc aaggaagcag aacggagaat cttgctcagc ccaccaagtg
     6481 ttctccagtg gagacttagc gcaagccatg atgttctcgt ggttttcctc aatgaacttg
     6541 atgcgctcag ggaacggaac cttatcgaca cccgcacagt ttgcaccgtg gattttcagc
     6601 cagtagtaac cttccttacc gattggttta cctttcgcca gcgtaagcag tcctttggtc
     6661 atatcgttac cttgcgggtt gaacattgac acagcgtaaa cacgaccgcg ccagtccatg
     6721 ttgtaaggga accagatggc cttatggtta gcaaacttat tggcttgctc aagcatgaac
     6781 tcaaggctga tacggcgaga cttgcgagcc ttgtccttgc ggtacacagc agcggcagca
     6841 cgtttccacg cggtgagagc ctcaggattc atgtcgatgt cttccggttt catcgggagt
     6901 tcttcacgct caatcgcagg gatgtcctcg accggacaat gcttccactt ggtgattacg
     6961 ttggcgaccg ctaggacttt cttgttgatt ttccatgcgg tgttttgcgc aatgttaatc
     7021 gctttgtaca cctcaggcat gtaaacgtct tcgtagcgca tcagtgcttt cttactgtga
     7081 gtacgcacca gcgccagagg acgacgaccg ttagcccaat agccaccacc agtaatgcca
     7141 gtccacggct taggaggaac tacgcaaggt tggaacatcg gagagatgcc agccagcgca
     7201 cctgcacggg ttgcgatagc ctcagcgtat tcaggtgcga gttcgatagt ctcagagtct
     7261 tgacctacta cgccagcatt ttggcggtgt aagctaacca ttccggttga ctcaatgagc
     7321 atctcgatgc agcgtactcc tacatgaata gagtcttcct tatgccacga agaccacgcc
     7381 tcgccaccga gtagaccctt agagagcatg tcagcctcga caacttgcat aaatgctttc
     7441 ttgtagacgt gccctacgcg cttgttgagt tgttcctcaa cgtttttctt gaagtgctta
     7501 gcttcaaggt cacggatacg accgaagcga gcctcgtcct caatggcccg accgattgcg
     7561 cttgctacag cctgaacggt tgtattgtca gcactggtta ggcaagccag agtggtctta
     7621 atggtgatgt acgctacggc ttccggcttg atttcctaca ggaactggaa ggctgtcggg
     7681 cgcttgccgc gcttagcttt cacttcctca aaccagtcgt tgatgcgtgc aatcatctta
     7741 gggagtaggg tagtgatgag aggcttggcg gcagcgttat ccgcaacctc accagcttta
     7801 agttgacgct caaacatctt gcggaagcgt gcttcaccca tctcgtaaga ctcatgctca
     7861 agggccaact gttcgcgagc taaacgctca ccgtaatggt cagccagagt gttgaacggg
     7921 atagcagcca gttcgatgtc agagaagtcg ttcttagcaa tgttaatggt attctagtgc
     7981 acggtaatca tggtcatggt taattcctcc tgttagccca aaaaacgggt atggagaaac
     8041 agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc
     8101 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
     8161 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
     8221 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
     8281 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
     8341 tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg cccatctggt
     8401 ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg accgaccgct
     8461 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca
     8521 gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag gagagattca
     8581 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc
     8641 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cggggttctt
     8701 tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa tcattaacgc
     8761 cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg agcaattgtt
     8821 actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata atcgggtacg
     8881 cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata tcgccagcgt
     8941 cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc agcagttagg
     9001 gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc tgcttttgag
     9061 caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta
     9121 tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc gtgccggttg
     9181 tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga atcagacaat
     9241 tgacggcttg acggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc
     9301 acattatgca tgccgcttcg ccttcgcgcg cgaattgatc tgctgcctcg cgcgtttcgg
     9361 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cctgcagcaa
     9421 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt
     9481 ggcagcagcc aactcagctt cctttcgggc tttgttattt gtagagctca tccatgccat
     9541 gtgtaatccc agcagcagtt acaaactcaa gaaggaccat gtggtcacgc ttttcgttgg
     9601 gatctttcga aagggcagat tgtgtcgaca ggtaatggtt gtctggtaaa aggacagggc
     9661 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacggat ccatcttcaa
     9721 tgttgtggcg aattttgaag ttagctttga ttccattctt ttgtttgtct gccgtgatgt
     9781 atacattgtg tgagttatag ttgtactcga gtttgtgtcc gagaatgttt ccatcttctt
     9841 taaaatcaat accttttaac tcgatacgat taacaagggt atcaccttca aacttgactt
     9901 cagcacgcgt cttgtagttc ccgtcatctt tgaaagatat agtgcgttcc tgtacataac
     9961 cttcgggcat ggcactcttg aaaaagtcat gccgtttcat atgatccgga taacgggaaa
    10021 agcattgaac accataagag aaagtagtga caagtgttgg ccatggaaca ggtagttttc
    10081 cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc
    10141 cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa
    10201 ctccagtgaa aagttcttct cctttactca tatgtatatc tccttcttaa agttaaacaa
    10261 aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg
    10321 gatcg
//
