LOCUS       sequence-389465-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB1 mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBpuro-ErbB1(DEL1011)
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(2760..2831,2832..4694,4695..4763,4764..6395)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLL*SSLSATSNNSTV
                     ACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQ
                     NPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQI
                     SLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     misc_feature    6473..7022
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6617..6634)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6844..6863
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7025..7624
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7025..7044)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7521..7541
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7651..7668)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7657..7881
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7928..7944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7952..7968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7976..7982,7983..8000,8001..8006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8021..8042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8276..8338
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8379..8399)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8379..8397)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8466..8472,8473..8490,8491..8496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8649..8666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8900..8919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9579..10370,10371..10439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10440..10544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagacca
     2761 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg
     2821 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt
     2881 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg
     2941 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga
     3001 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt
     3061 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag
     3121 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac
     3181 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga
     3241 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc
     3301 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg
     3361 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg
     3421 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca
     3481 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca
     3541 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc
     3601 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg
     3661 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag
     3721 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag
     3781 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa
     3841 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct
     3901 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa
     3961 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg
     4021 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg
     4081 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg
     4141 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt
     4201 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg
     4261 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca
     4321 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc
     4381 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag
     4441 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc
     4501 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg
     4561 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc
     4621 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc
     4681 ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg
     4741 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga
     4801 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc
     4861 aagctctctt gaggatcttg aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg
     4921 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc
     4981 ccgtcgctat caaggaatta agagaagcaa catctccgaa agccaacaag gaaatcctcg
     5041 atgaagccta cgtgatggcc agcgtggaca acccacatgt ctgtcggctg ctgggcatct
     5101 gcctcacctc caccgtgcaa ctcatcacgc agctcatgcc cttcggctgc ctcctggact
     5161 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga
     5221 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca
     5281 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac
     5341 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga
     5401 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg
     5461 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca
     5521 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca
     5581 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt
     5641 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca
     5701 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc
     5761 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc
     5821 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgtaaagc tctctgagtg
     5881 caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca
     5941 tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg
     6001 acagcataga cgacaccttc ctcccagtgc ctgaatacat aaaccagtcc gttcccaaaa
     6061 ggcccgctgg ctctgtgcag aatcctgtct atcacaatca gcctctgaac cccgcgccca
     6121 gcagagaccc acactaccag gacccccaca gcactgcagt gggcaacccc gagtatctca
     6181 acactgtcca gcccacctgt gtcaacagca cattcgacag ccctgcccac tgggcccaga
     6241 aaggcagcca ccaaattagc ctggacaacc ctgactacca gcaggacttc tttcccaagg
     6301 aagccaagcc aaatggcatc tttaagggct ccacagctga aaatgcagaa tacctaaggg
     6361 tcgcgccaca aagcagtgaa tttattggag catgagcggc cgctctagag tcaactaact
     6421 taagctagca acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact
     6481 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6541 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6601 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6661 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6721 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6781 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6841 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6901 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6961 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     7021 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     7081 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     7141 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7201 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7261 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7321 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7381 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7441 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7501 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7561 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7621 ctgatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     7681 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7741 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7801 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7861 gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagccca agcttggcgt
     7921 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7981 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8041 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct
     8101 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     8161 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     8221 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     8281 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     8341 aatctagtat acgcgtttaa ttaatccagc ttttgttccc tttagtgagg gttaattgcg
     8401 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10561 gtgccac
//
